BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,977	C→T	A2T (GCC→ACC)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,977	0	C	T	100.0%	478.2 / NA	130	A2T (GCC→ACC)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (64/66); total (64/66)

Only 100 of 130 total aligned reads displayed.

TTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCA  >  NC_000913/4310684‑4311270

ttCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCataaa                                                                                                                                                                                                                                                                                                 <  1:146977/300‑1 (MQ=255)

 tCCAGTACGGTTAAGTCATCAATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCataaat                                                                                                                                                                                                                                                                                                <  2:246035/300‑1 (MQ=255)

         ggTTAATTCATCAATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTGTGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACctctc                                                                                                                                                                                                                                                                                        <  1:746776/300‑1 (MQ=255)

              aTTCATCAATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTATGCAc                                                                                                                                                                                                                                                                                   >  1:640687/1‑300 (MQ=255)

                 catcaATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGaa                                                                                                                                                                                                                                                                                >  1:66560/1‑300 (MQ=255)

                 catcaATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGGAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGaa                                                                                                                                                                                                                                                                                <  2:628738/300‑1 (MQ=255)

                      aTAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATCTGGCGTGGTCATACATAACCTCTCTGCACGAAATATc                                                                                                                                                                                                                                                                           >  1:334693/1‑300 (MQ=255)

                         aCGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTATGCACGAAATATTGta                                                                                                                                                                                                                                                                        >  2:251037/1‑300 (MQ=255)

                          cGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTACTGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACCTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCACGCCATCGATATCTATGGCGTGGTCATAAATACACTCGATGCACGCAATATCGtat                                                                                                                                                                                                                                                                       >  2:387536/1‑300 (MQ=255)

                                gTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGc                                                                                                                                                                                                                                                                 >  1:636678/1‑300 (MQ=255)

                                             aatCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTNAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCANGAAATATCGTATTATTGCCTGATGGCTACAt                                                                                                                                                                                                                                                    <  2:586378/300‑1 (MQ=255)

                                             aatCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACAt                                                                                                                                                                                                                                                    <  1:738190/300‑1 (MQ=255)

                                                    aTACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCAACAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAg                                                                                                                                                                                                                                             >  2:494273/1‑300 (MQ=255)

                                                    aTACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAg                                                                                                                                                                                                                                             >  1:340305/1‑300 (MQ=255)

                                                     tACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAgg                                                                                                                                                                                                                                            <  2:63509/300‑1 (MQ=255)

                                                         gAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTa                                                                                                                                                                                                                                        >  2:776584/1‑300 (MQ=255)

                                                            ttGTGTCGCTAATTTATGATCCAGCTTGTTATAGCTAATGGTATTAATATTATGGGTGCCTGTGGTCTGCGCATATATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGGGGTTATAAATAACCTCTCTGCACTAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTGCGt                                                                                                                                                                                                                                     <  2:263182/300‑1 (MQ=255)

                                                            ttGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGTTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                                                                                                                                                                                                     <  2:781124/300‑1 (MQ=255)

                                                            ttGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                                                                                                                                                                                                     <  1:744055/300‑1 (MQ=255)

                                                            ttGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATCGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCANGATAAACCGTTAAATTAACCGACTTTNATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGt                                                                                                                                                                                                                                     <  2:531897/300‑1 (MQ=255)

                                                                   gCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGtt                                                                                                                                                                                                                              <  1:639102/300‑1 (MQ=255)

                                                                         ttATGATCCAGCTTGTTATAGCTAATGTTATGAATGTTAATGGTGCTTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTttg                                                                                                                                                                                                                        <  2:617187/300‑1 (MQ=255)

                                                                         ttATAATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGTTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTttg                                                                                                                                                                                                                        <  2:277848/300‑1 (MQ=255)

                                                                             gATCGAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTgg                                                                                                                                                                                                                    >  1:91132/1‑300 (MQ=255)

                                                                                  aGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGNCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGa                                                                                                                                                                                                               >  1:556688/1‑300 (MQ=255)

                                                                                  aGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGa                                                                                                                                                                                                               >  1:389959/1‑300 (MQ=255)

                                                                                   ggttGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCACTAACGTGAATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCAGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGAt                                                                                                                                                                                                              >  2:502118/3‑300 (MQ=255)

                                                                                       gTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGa                                                                                                                                                                                                          >  1:697614/1‑300 (MQ=255)

                                                                                       gTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGCATTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCNTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGa                                                                                                                                                                                                          >  2:593786/1‑300 (MQ=255)

                                                                                        ttATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                                                                                                                                                                                                         <  2:82495/300‑1 (MQ=255)

                                                                                        ttATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                                                                                                                                                                                                         <  1:503583/300‑1 (MQ=255)

                                                                                        ttATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATGTTGTTGGCCGGATAAGac                                                                                                                                                                                                         <  1:694847/300‑1 (MQ=255)

                                                                                        ttATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTCCATTAGCGTGGATTTACCCGCGCCATTTTCGCCTATTAAGGCATGTATTTCACCAGGGTAAACCGTTNAATTAACCGACTGTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGac                                                                                                                                                                                                         <  2:205096/300‑1 (MQ=255)

                                                                                                  tGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACNGGACCAAAGGACTTGCCGATCCCCGCNATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAg                                                                                                                                                                                               <  2:556022/300‑1 (MQ=255)

                                                                                                    ttattaATGGTAATGGTGCCTTTGGTCGGCGCAGGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGca                                                                                                                                                                                             <  2:432149/300‑1 (MQ=255)

                                                                                                          atggtaatggtGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcatcatca                                                                                                                                                                                       <  1:463821/300‑1 (MQ=255)

                                                                                                                atggtGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTTTCTGCACGAAATATTGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGc                                                                                                                                                                                 <  2:230141/300‑1 (MQ=255)

                                                                                                                  ggtGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcat                                                                                                                                                                               <  2:781743/300‑1 (MQ=255)

                                                                                                                      ccTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCAtt                                                                                                                                                                           >  1:155133/1‑300 (MQ=255)

                                                                                                                            gTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGttt                                                                                                                                                                     <  1:451140/300‑1 (MQ=255)

                                                                                                                            gTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTNTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGcactgtctcttatacacatctcgcag                                                                                                                                                                     <  1:563986/300‑25 (MQ=255)

                                                                                                                              cGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTATACGTTCAGGTTATTTTGTTGGCAGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGttttc                                                                                                                                                                   >  1:86343/1‑299 (MQ=255)

                                                                                                                                gCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGNTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTAtt                                                                                                                                                                 >  2:603510/1‑300 (MQ=255)

                                                                                                                                gCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGACTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCAACAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCNTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTAtt                                                                                                                                                                 >  2:718264/1‑300 (MQ=255)

                                                                                                                                 cTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTg                                                                                                                                                                <  1:427120/300‑1 (MQ=255)

                                                                                                                                 cTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTg                                                                                                                                                                <  2:380579/300‑1 (MQ=255)

                                                                                                                                   cATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAg                                                                                                                                                              >  1:8363/1‑300 (MQ=255)

                                                                                                                                   cATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTAATAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTATGCACGAAATATCGTATTATTGCCTGATGGCTACACTTATCAGGTCTAAGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTAtcgcg                                                                                                                                                              >  2:12916/1‑296 (MQ=255)

                                                                                                                                    aTGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGt                                                                                                                                                             >  1:733750/1‑300 (MQ=255)

                                                                                                                                        aTTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGcatctgtctcttatacacatctcgc                                                                                                                                                         <  1:32083/300‑22 (MQ=255)

                                                                                                                                             ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCTGACCAANGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGANATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGAt                                                                                                                                                    <  2:638979/300‑1 (MQ=255)

                                                                                                                                             ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTGGCCGATCCCCGCCATCGATATATATGGCGTGGTCAGAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGAt                                                                                                                                                    <  1:56877/300‑1 (MQ=255)

                                                                                                                                             ggATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGAt                                                                                                                                                    >  1:681081/1‑300 (MQ=255)

                                                                                                                                             ggATAAAACTTCCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGAt                                                                                                                                                    >  1:379836/1‑300 (MQ=255)

                                                                                                                                             ggATAAAAATTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGAt                                                                                                                                                    <  1:406650/300‑1 (MQ=255)

                                                                                                                                                  aaaCTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAAt                                                                                                                                               <  2:345750/300‑1 (MQ=255)

                                                                                                                                                  aaaCTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAAt                                                                                                                                               <  1:666202/300‑1 (MQ=255)

                                                                                                                                                  aaaCTTTCATGAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAANGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAAt                                                                                                                                               <  1:549372/300‑1 (MQ=255)

                                                                                                                                                    aCTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCg                                                                                                                                             >  2:148609/1‑300 (MQ=255)

                                                                                                                                                       ttCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAAGAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGAcc                                                                                                                                          <  1:41130/300‑1 (MQ=255)

                                                                                                                                                             aGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTa                                                                                                                                    >  2:79997/1‑300 (MQ=255)

                                                                                                                                                             aGCGTGGATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTa                                                                                                                                    >  1:101057/1‑300 (MQ=255)

                                                                                                                                                                 tGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAAtt                                                                                                                                <  1:169673/300‑1 (MQ=255)

                                                                                                                                                                  ggATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCNCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTc                                                                                                                               <  1:582497/300‑1 (MQ=255)

                                                                                                                                                                  ggATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCCGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTc                                                                                                                               >  2:29445/1‑300 (MQ=255)

                                                                                                                                                                  ggATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTc                                                                                                                               >  1:511172/1‑300 (MQ=255)

                                                                                                                                                                  ggATTTACCCGCGCCATTTTCTACTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTc                                                                                                                               >  2:502529/1‑300 (MQ=255)

                                                                                                                                                                  ggATTTACCCGCGCCATTTCCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGACGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTc                                                                                                                               <  1:148487/300‑1 (MQ=255)

                                                                                                                                                                               ccATTTTCTCCTATTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATcc                                                                                                                  <  1:667133/300‑1 (MQ=255)

                                                                                                                                                                               ccATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATcc                                                                                                                  <  1:33578/300‑1 (MQ=255)

                                                                                                                                                                                    ttCTCCTATTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTGAATGCGTGAACCGGACCAAAGGACTTGGCGATCCCCGCCATCGATATATATGGCGTGGTCAGAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCGACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCTGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCgg                                                                                                             <  2:690937/300‑1 (MQ=255)

                                                                                                                                                                                          tAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCTGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAAc                                                                                                       <  2:401465/300‑1 (MQ=255)

                                                                                                                                                                                          tAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTATACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTAATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGAACCGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATacc                                                                                                       >  2:418413/1‑298 (MQ=255)

                                                                                                                                                                                            gtagtGCATGTATTTCACCAGGAGAAACCGGTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGCGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCt                                                                                                     <  2:631769/296‑1 (MQ=255)

                                                                                                                                                                                              aaTGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTg                                                                                                   >  1:403949/1‑300 (MQ=255)

                                                                                                                                                                                                     gTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGAtt                                                                                            >  1:119382/1‑300 (MQ=255)

                                                                                                                                                                                                           cACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAg                                                                                      >  1:249806/1‑300 (MQ=255)

                                                                                                                                                                                                              cAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGt                                                                                   <  1:176067/300‑1 (MQ=255)

                                                                                                                                                                                                                ggATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCa                                                                                 >  1:436916/1‑300 (MQ=255)

                                                                                                                                                                                                                ggATAAACCGTTAAATTAAACGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTATACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATCCAGTTTTATTGAGTGAACAGGATTGAATCGAACAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCa                                                                                 >  2:241194/1‑300 (MQ=255)

                                                                                                                                                                                                                   tAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAAcc                                                                              <  1:696702/300‑1 (MQ=255)

                                                                                                                                                                                                                       ccGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCa                                                                          >  2:498816/1‑300 (MQ=255)

                                                                                                                                                                                                                       ccGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCa                                                                          >  1:258702/1‑300 (MQ=255)

                                                                                                                                                                                                                                tAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGAc                                                                 <  1:531916/300‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaCCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATTGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATccgc                                              <  1:73981/300‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaCCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCCACCATCAGCTTCAGACCCGTTGCGCCGATATccgc                                              >  1:387933/1‑300 (MQ=255)

                                                                                                                                                                                                                                                        gACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCAGGGt                                         >  1:473036/1‑300 (MQ=255)

                                                                                                                                                                                                                                                          ccAAAGGACTTGCCGATCCCCGCCATCGATATATCTGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTACATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCCGACCCGTTGCGCCGATATCCGCCGAGTTc                                       >  2:520883/1‑300 (MQ=255)

                                                                                                                                                                                                                                                          ccAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTc                                       >  2:519594/1‑300 (MQ=255)

                                                                                                                                                                                                                                                               ggACTTGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGc                                  <  1:484568/300‑1 (MQ=255)

                                                                                                                                                                                                                                                               ggACTTGCCGAGCCCCGCCATTGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCGTCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCGCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGc                                  <  1:256801/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                    tGCCGATCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCTGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCg                             <  2:10873/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                          tCCCCGCCATCGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGGATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTNGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCgg                       <  2:708537/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                    cGATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTcc             <  1:775256/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                     gATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGCTTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCg            >  2:689462/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                     gATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCg            >  2:796001/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                     gATATATATGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGACGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCg            >  2:330781/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                     gATATATATGGCGTGGTCATAAATAACCTCTATGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGtacg            >  2:428603/1‑297 (MQ=255)

                                                                                                                                                                                                                                                                                        atatatGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATNATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTNCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCACCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCt         >  2:648024/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                          atatGGCGTGGTCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTc       >  1:85415/1‑300 (MQ=255)

TTCCAGTACGGTTAATTCATCAATAACGCTGAGTTCCTGATAAATAATCCCGATACCGAGTTGTGCCGCTAATTTATGATCCAGCTTGTTATAGCTAATGTTATTAATGGTAATGGTGCCTTTGGTCGGCTCATGTATTCCGGATAAAACTTTCATTAGCGTGGATTTACCCGCGCCATTTTCTCCTAGTAATGCATGTATTTCACCAGGATAAACCGTTAAATTAACCGACTTTAATGCGTGAACCGGACCAAAGGACTTGCCGATCCCCGCCATCGATATATATGGCGTGGCCATAAATAACCTCTCTGCACGAAATATCGTATTATTGCCTGATGGCTACATTTATCAGGTCTACGTTCAGGTTATTTTGTTGGCCGGATAAGACACTTCATTAGCATCATCATCCGGCATCATTCAGTTTTATTGAGTGACCAGGATTGAATCGACCAGTTTAAATTCCGGTGCTTTATCCAGCGGGATAACCTTGCCGGATTTCTCAGCGTCAACCATCAGCTTCAGACCCGTTGCGCCGATATCCGCCGGGTTCTGGGCAACCGTCGCGGTCATTTGTCCGGCTTCCACCA  >  NC_000913/4310684‑4311270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`