Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,829,039	G→A	L232L (CTC→CTT)	astD ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,829,039	0	G	A	100.0%	179.3 / NA	50	L232L (CTC→CTT)	astD	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/22);  total (28/22)

CACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGC  >  NC_000913/1828745‑1829333                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       caccTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCACTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCGTCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTACGGCTGACCGGAAAGCTg                                                                                                                                                                                                                                                                                                   >  2:112384/1‑300 (MQ=255)      gctgTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGCCCGGCTGTGACAAACGCCGACTGAATGGTCGGATGGACGGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGAATTATTACCGACCATCTCAAGGGCGAGAATTTTCTCCGGCTGATAGGAAAGCTGGCGAt                                                                                                                                                                                                                                                                                              >  2:13565/1‑300 (MQ=255)                  gTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGc                                                                                                                                                                                                                                                                                  >  1:182382/1‑300 (MQ=255)                  gTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTAAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGc                                                                                                                                                                                                                                                                                  >  1:159573/1‑300 (MQ=255)                  gTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAANAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATNGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGc                                                                                                                                                                                                                                                                                  >  2:639868/1‑300 (MQ=255)                   ttCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCTGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGcc                                                                                                                                                                                                                                                                                 <  2:466029/300‑1 (MQ=255)                    tCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTgtctcttatacacatctcgc                                                                                                                                                                                                                                                                                <  1:464272/300‑20 (MQ=255)                       gAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTa                                                                                                                                                                                                                                                                             >  1:651111/1‑300 (MQ=255)                             aGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGgcg                                                                                                                                                                                                                                                                       <  2:490960/300‑1 (MQ=255)                                         aaCGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGTTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTTGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAAc                                                                                                                                                                                                                                                           <  2:158803/300‑1 (MQ=255)                                             gCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTAAGATGGACAGCAGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTAAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCa                                                                                                                                                                                                                                                       >  2:72158/1‑300 (MQ=255)                                                     tCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCg                                                                                                                                                                                                                                               >  1:280667/1‑300 (MQ=255)                                                              cAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGAtcctcc                                                                                                                                                                                                                                      >  1:283805/1‑300 (MQ=255)                                                                  tGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAgcg                                                                                                                                                                                                                                  <  1:28908/300‑1 (MQ=255)                                                                     ccGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACACATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCGGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTCCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGTTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCAc                                                                                                                                                                                                                               <  1:78444/300‑1 (MQ=255)                                                                        gCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCa                                                                                                                                                                                                                            >  1:605717/1‑300 (MQ=255)                                                                             aaTCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGNCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGcc                                                                                                                                                                                                                       <  1:597113/300‑1 (MQ=255)                                                                             aaTCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGTCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGcc                                                                                                                                                                                                                       <  2:278863/300‑1 (MQ=255)                                                                             aaTCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGcc                                                                                                                                                                                                                       <  1:688930/300‑1 (MQ=255)                                                                                 gctggctgACGGCAACCAGACGAACAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACNTCATCGATAATTAGCGGATTATTACCNCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTTTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCACCGCACTCAGCGCCTGAc                                                                                                                                                                                                                   <  2:547870/300‑1 (MQ=255)                                                                                          cGGCAACCAGACGAGCAAGAAACGCCTCGCCCTGCGCCCAGCTTTTCAGCAATAGATGGCGGACGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGAGGGAACTTTCTCCGGCTGACCGAAACGTTGGCGATGTAACTGGTAGCCTGTATTGGCGCTACCGGTAACCAGCAAACCGTCGAGATCCTCCAGCGCCCCCGGCGCCTGACACATTTCAc                                                                                                                                                                                                          >  2:312948/1‑300 (MQ=255)                                                                                          cGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTAAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAc                                                                                                                                                                                                          >  1:199469/1‑300 (MQ=255)                                                                                             cAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCAcgcc                                                                                                                                                                                                       <  2:525152/300‑1 (MQ=255)                                                                                              aaCCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGTATGACCCGTTTCAcgccc                                                                                                                                                                                                      >  2:22224/1‑300 (MQ=255)                                                                                                       aGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCAc                                                                                                                                                                                             >  2:323506/1‑300 (MQ=255)                                                                                                              aaCGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTc                                                                                                                                                                                      <  1:64421/300‑1 (MQ=255)                                                                                                                      gCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGcc                                                                                                                                                                              >  1:535973/1‑300 (MQ=255)                                                                                                                      gCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTAAAGGGCGAGAATTTTCTACGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACAGTCGAGATCCTACAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGcc                                                                                                                                                                              >  2:85448/1‑300 (MQ=255)                                                                                                                                                   tgcgggcgCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAAc                                                                                                                                                 <  1:772533/299‑1 (MQ=255)                                                                                                                                                          gcAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAAGGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTAc                                                                                                                                          <  1:605933/300‑1 (MQ=255)                                                                                                                                                                      ttGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACt                                                                                                                              >  1:699816/1‑300 (MQ=255)                                                                                                                                                                             gCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGgt                                                                                                                       >  1:447770/1‑300 (MQ=255)                                                                                                                                                                             gCTGGGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACNTTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCNTAAACGCATTACCGCTTCGCCACTCCACGgt                                                                                                                       <  2:570801/300‑1 (MQ=255)                                                                                                                                                                                    cAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATCACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTAACGCTTCGACACTCCACGGTGTCAGtt                                                                                                                >  2:73400/1‑300 (MQ=255)                                                                                                                                                                                          ccGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTgg                                                                                                          >  2:624696/1‑300 (MQ=255)                                                                                                                                                                                                         gATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATgg                                                                                           <  1:166945/300‑1 (MQ=255)                                                                                                                                                                                                                    gcgTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGcc                                                                                >  1:204872/1‑300 (MQ=255)                                                                                                                                                                                                                                  cACCTCATCGATAATTATCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTNTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCANGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAAc                                                                  <  2:743033/300‑1 (MQ=255)                                                                                                                                                                                                                                       cATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAatat                                                             <  2:304898/300‑1 (MQ=255)                                                                                                                                                                                                                                       cATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAatat                                                             >  1:260409/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAAt                                              >  1:535481/1‑300 (MQ=255)                                                                                                                                                                                                                                                         tattaCCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGAc                                           <  1:38073/300‑1 (MQ=255)                                                                                                                                                                                                                                                          attaCCGCCCATCTAAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGaac                                          >  1:410676/1‑298 (MQ=255)                                                                                                                                                                                                                                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTTCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGACCAGGGAAATtat                               >  1:250265/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGACCAGGGAAATtat                               >  1:148427/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     tctcAAGGGCGAGAATTTTCTCCGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGAACCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGNCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCCGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGAACAGGGAAATtat                               >  2:185546/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          ccGGCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCa          <  2:41112/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gCTGACCGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGc       <  1:396054/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ccGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGc  >  1:313924/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  ccGGAAAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACAATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGc  <  2:484603/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       CACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTGCGCCCCGCTTTTCAGCAATAAACGGCGGGCGCAGGTGCAGCGTTGACCGGCTGTGACAAACGCCGACTGAATGGTCAGATGGACAGCCGCGTCGATATCCGCCACCTCATCGATAATTAGCGGATTATTACCGCCCATCTCAAGGGCGAGAATTTTCTCCGGCTGACCGGAGAGCTGGCGATGCAACTGGTAGCCTGTATTGGCGCTACCGGTAAACAGCAAACCGTCGAGATCCTCCAGCGCACTCAGCGCCTGACCCGTTTCACGCCCGCCCTGCACCAGGTTCAGCACGCCTGGCGGCAAGCCAGCCTGCTGCCATAAACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGC  >  NC_000913/1828745‑1829333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑