Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,546,804	T→A	D44E (GAT→GAA)	murP →	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,546,804	0	T	A	100.0%	307.6 / NA	87	D44E (GAT→GAA)	murP	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (43/44);  total (43/44)

ATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGT  >  NC_000913/2546513‑2547090                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                               aTGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGGTGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCNGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTNCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGAGGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAatat                                                                                                                                                                                                                                                                                        >  2:644986/1‑300 (MQ=255) aTGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGNACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAatat                                                                                                                                                                                                                                                                                        >  1:598678/1‑300 (MQ=255) aTGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAatat                                                                                                                                                                                                                                                                                        >  2:784865/1‑300 (MQ=255)  tGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATc                                                                                                                                                                                                                                                                                       <  1:779224/300‑1 (MQ=255)      gcgcAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCATAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCaaaa                                                                                                                                                                                                                                                                                   >  1:654955/1‑300 (MQ=255)      gcgcAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGNGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACANGACAGTTCACTGGTTGAACCCAATATCaaaa                                                                                                                                                                                                                                                                                   <  2:587591/300‑1 (MQ=255)           gagCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCGTTGTGATGGTGCTGAAAAATCTCGATGTCGCAGGAGCTAAAAAACGCCTAGATCAACACGGCGGCTTTATTCGTCAAGTTTTAGACAAGGAATAACCCATGGCCAAAGGGATCAGCAGTGAACTTCTGCACACCATTCTTACCCGTGTCGGCGGAATGGGAACTATCGCAAGTTGTGGTACTTGTATGACGCGCATGTGTCTGGGTGTACATGACAGTTCACTGGTTGAAACCAATATCAAACCGCTg                                                                                                                                                                                                                                                                              >  2:116200/1‑300 (MQ=255)           gagCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTg                                                                                                                                                                                                                                                                              >  1:90676/1‑300 (MQ=255)           gagCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGACATTGTGATGGTGCTGAAAAATCTAGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGGGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTg                                                                                                                                                                                                                                                                              >  2:50475/1‑300 (MQ=255)                ggcggaagcggCGGTAATTGCTTGCGAGCGCAACTGTAAAACGTCCATTGTGATGGTGCTGAAAAATCTCGATGCCACAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCGGGAAgg                                                                                                                                                                                                                                                                         <  1:48792/299‑1 (MQ=255)                agcggaagcggCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTTTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAgg                                                                                                                                                                                                                                                                         <  1:337457/300‑1 (MQ=255)                agcggaagcggCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCTGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAgg                                                                                                                                                                                                                                                                         <  1:298850/300‑1 (MQ=255)                    gaagcggCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTAGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTNAGACAAGGAATAACCCATGGCCAAAGNGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCAATGGTTGAACCCAATCTCACAACTCTGGCAGGAGTg                                                                                                                                                                                                                                                                     >  2:553097/1‑300 (MQ=255)                                      tgcGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCGGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTGAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGGGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGAcc                                                                                                                                                                                                                                                   <  2:304114/300‑1 (MQ=255)                                              cAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGTTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCa                                                                                                                                                                                                                                           >  2:19049/1‑300 (MQ=255)                                                 cTGTAAAACGGCCATTGTGATGGTGCTGCAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATTAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGTGTCTGGGTGTACATGACCGTTTACTGGTTGAACCCAATATCAAAACGCTGGCAGGCGTGAAGGGTGTCATTTTGACCcgcggctaggc                                                                                                                                                                                                                                        >  2:20314/1‑293 (MQ=255)                                                 cTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGCCCAGCGACCAGGt                                                                                                                                                                                                                                        >  1:114961/1‑300 (MQ=255)                                                 cTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGt                                                                                                                                                                                                                                        >  2:729291/1‑300 (MQ=255)                                                       aaCGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGt                                                                                                                                                                                                                                  <  1:498858/300‑1 (MQ=255)                                                        aCGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTc                                                                                                                                                                                                                                 <  1:666296/300‑1 (MQ=255)                                                        aCGGCCATTGTGAGGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACTGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGAGCAGCAGTGAACTTNTGAACACCATTCTTACCCGTGTCGGCGGACCGGTAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTc                                                                                                                                                                                                                                 <  2:201443/300‑1 (MQ=255)                                                              aTTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGACAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACAAGGTGCAGGTCGttttt                                                                                                                                                                                                                           >  2:125303/1‑300 (MQ=255)                                                                             aaaagTCTCGATGCCGTAGAAGCTAAAAAACGCCTAGATCAACACGTCGGCGTTCTTCGTCAGGTTTTAGACAAGGAATAACCCAGGGCCAAAGAGAGCAGCAGTGAACTTCTGAACACCACTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGTCTGCGTCTGGGTGTACATGACAGTGCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTGTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAgcg                                                                                                                                                                                                            <  2:151372/300‑1 (MQ=255)                                                                             aaaaaTCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAgcg                                                                                                                                                                                                            <  1:66490/300‑1 (MQ=255)                                                                              aaaaTCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAgcgc                                                                                                                                                                                                           <  2:303358/300‑1 (MQ=255)                                                                                   cttgATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGGCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGAGCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGt                                                                                                                                                                                                      <  2:355679/297‑1 (MQ=255)                                                                                         gCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGC‑TGGTAAAGCGCATCGTGCCGCaa                                                                                                                                                                                               <  1:789648/300‑1 (MQ=255)                                                                                            gCAGAAGCTAAAAAACGCCTGGATCAACACGNCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGNACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTCAAGCGCATCGTGCCGCaaaa                                                                                                                                                                                             >  2:583865/1‑300 (MQ=255)                                                                                                           cGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAgctg                                                                                                                                                                              >  1:250516/1‑300 (MQ=255)                                                                                                                ggATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTNGTGGTAACTGTATGACGCGCCTGCGTNTGGGTGTACATGACAGTTCACTGGTTGAACCCAAGATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTggg                                                                                                                                                                         >  2:537957/1‑300 (MQ=255)                                                                                                                 gATCAACACTGCTGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCTGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGTTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTACTGGga                                                                                                                                                                        <  2:153877/300‑1 (MQ=255)                                                                                                                 gATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCNTCTGGGTGTACATGACAGTTCACTGGNTGAACCCAATATCAAAACGCTGTAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGga                                                                                                                                                                        <  2:542176/300‑1 (MQ=255)                                                                                                                 gATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGTTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGga                                                                                                                                                                        <  2:260369/300‑1 (MQ=255)                                                                                                                   tCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCTGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGgaga                                                                                                                                                                      <  1:400759/300‑1 (MQ=255)                                                                                                                   tCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGgaga                                                                                                                                                                      <  1:211248/300‑1 (MQ=255)                                                                                                                    cAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGaa                                                                                                                                                                     >  1:211934/1‑300 (MQ=255)                                                                                                                        acGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCAc                                                                                                                                                                 <  1:364246/300‑1 (MQ=255)                                                                                                                              gcTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCACAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTAGTTTTTGGGCCTGGTAAAGTGCATCATGCCGCAACAGCGACGGGCGAGCTGATGGGAGAAGCACCGATAc                                                                                                                                                           >  2:164002/1‑300 (MQ=255)                                                                                                                              gcTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTAc                                                                                                                                                           >  2:164093/1‑300 (MQ=255)                                                                                                                                tttATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCATTGAACTTCTGAACACCACTCTTACCCGTGTCGGCGGACCGGGAAACATCGCCAGTTGTGGTAACTGTATTACGCGCCTGCGTCTGGGTGTACATGACGGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGTTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAACTGCTGTGAGAAGCACCGGTACAg                                                                                                                                                         <  2:8501/300‑1 (MQ=255)                                                                                                                                   aTTCGTCATGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGTAGAAGCACCGGTACAGGAt                                                                                                                                                      <  2:297110/300‑1 (MQ=255)                                                                                                                                   aTTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAGATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGAt                                                                                                                                                      <  1:502843/300‑1 (MQ=255)                                                                                                                                   aTTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGAt                                                                                                                                                      <  1:403311/300‑1 (MQ=255)                                                                                                                                   aTTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAAATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCTGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGAt                                                                                                                                                      <  2:126603/300‑1 (MQ=255)                                                                                                                                   aTTCGTCAGGTTTTAGACAAGGAAGAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGTAAATATCGCCAGTTGTGTTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGTAGAAACACCGGTACAgaat                                                                                                                                                      <  2:679100/300‑4 (MQ=255)                                                                                                                                     tCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGc                                                                                                                                                    <  1:293007/300‑1 (MQ=255)                                                                                                                                            gTTTTAGACAAGGAATACCCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGAACAGGTGCAGGTCGTTTTTGGGCATGGTAAAGCGCATCGTGCAGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGaa                                                                                                                                             >  2:147528/1‑300 (MQ=255)                                                                                                                                            gTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCNGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCNTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGaa                                                                                                                                             >  2:647720/1‑300 (MQ=255)                                                                                                                                                      aGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGCAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGAAGAAATCGCCGccc                                                                                                                                   >  2:311688/1‑300 (MQ=255)                                                                                                                                                             aCCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACaa                                                                                                                            >  1:339032/1‑300 (MQ=255)                                                                                                                                                                cATGGCCAAAGAGATCAGCATTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCGGATGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACg                                                                                                                         <  2:123022/300‑1 (MQ=255)                                                                                                                                                                cATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACg                                                                                                                         <  1:351583/300‑1 (MQ=255)                                                                                                                                                                            gaTCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGc                                                                                                             <  1:532505/300‑1 (MQ=255)                                                                                                                                                                            gaTCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCAGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGc                                                                                                             <  2:70324/300‑1 (MQ=255)                                                                                                                                                                            aaTCAGCAGTGAACTTCTGAACACCATTCTTACCCTTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGNTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAANTCGCCGCCCAGAACAAACGTCAGTTAAAAGc                                                                                                             <  2:587558/299‑1 (MQ=255)                                                                                                                                                                                      gAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCTGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCCGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAAcc                                                                                                   <  2:692640/300‑1 (MQ=255)                                                                                                                                                                                           tCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCgg                                                                                              >  1:243248/1‑300 (MQ=255)                                                                                                                                                                                                acacCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGc                                                                                         >  1:726316/1‑300 (MQ=255)                                                                                                                                                                                                   ccATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCNGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCAAAACAAACCTCAGGCGTGcaac                                                                                      >  1:563955/1‑300 (MQ=255)                                                                                                                                                                                                     aTTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGcaacaa                                                                                    >  2:774694/1‑300 (MQ=255)                                                                                                                                                                                                     aTTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGcaacaa                                                                                    >  1:131827/1‑300 (MQ=255)                                                                                                                                                                                                              cGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGcc                                                                           >  1:727086/1‑300 (MQ=255)                                                                                                                                                                                                                       ggACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGcc                                                                  <  1:10089/300‑1 (MQ=255)                                                                                                                                                                                                                             ggAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATc                                                            <  1:702128/300‑1 (MQ=255)                                                                                                                                                                                                                                 atatCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTNCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGNAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCa                                                        >  2:642966/1‑300 (MQ=255)                                                                                                                                                                                                                                    tCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCAcgc                                                     <  1:41080/300‑1 (MQ=255)                                                                                                                                                                                                                                    tCGCCAGTTGTGGGAACTGTATGACACGCCTGCGTCTGGGTGTACATGACAGTGCAATGGTTGAACCCAATATCAAAACGCTGGAGGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCACCTTCAcgc                                                     <  2:467661/300‑1 (MQ=255)                                                                                                                                                                                                                                     cGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCNGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCAcgcc                                                    <  1:550394/300‑1 (MQ=255)                                                                                                                                                                                                                                          gTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGACCAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGa                                               >  1:793592/1‑300 (MQ=255)                                                                                                                                                                                                                                          gTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGa                                               >  1:743018/1‑300 (MQ=255)                                                                                                                                                                                                                                            tgtgGTAACGGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATc                                             <  1:695915/300‑1 (MQ=255)                                                                                                                                                                                                                                                    cTGTCTGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAACAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCAGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGttt                                     >  1:719310/1‑300 (MQ=255)                                                                                                                                                                                                                                                    cTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCNCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGttt                                     >  1:573245/1‑300 (MQ=255)                                                                                                                                                                                                                                                    cTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGttt                                     >  2:527854/1‑300 (MQ=255)                                                                                                                                                                                                                                                    cTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCACAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTAGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGttt                                     >  2:782495/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTACGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTa                                   >  1:382268/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACNAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTctgc                    >  2:715300/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTctgc                    >  2:429355/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gtACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGNGATGAGCGAGCTGCTGGGAGAAGCACNGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTgggg            >  2:554934/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gtACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTgggg            <  2:778945/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aCATGACAGTTCACTGTTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGAt          <  1:132882/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aCATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGAt          >  1:42177/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               aCATGACAGTTCACTGGGTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAACCAAACAAACCTCCGGCGTGCAACAACTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCGGGGGAt          <  2:727244/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    aCAGTTCACTGGTTGAACCCAATATCAAAACGTTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTGGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGGACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGa     <  2:347527/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    aCAGTTCACTGGTTAAACCCAATATCAAAACGCTGGAAGGCGTGAAGTGCGTCGTTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCGGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCATTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGa     <  2:1921/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGt  >  2:782886/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       gTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTCGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTAGCCAAATTCGCCACCATCTTCACGCCACTGATCCACAGTTTTATTGCCGACGGTCTGCTGCTGGGGATaacaacgt  >  2:462088/1‑293 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                               ATGTAGCGCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCGACGT  >  NC_000913/2546513‑2547090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑