Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,399,245 G→A T245I (ACC→ATC)  mreC ← cell shape determining protein MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,399,2450GA98.7% 268.7 / NA 77T245I (ACC→ATC) mreCcell shape determining protein MreC
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (43/33);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCC  >  NC_000913/3398947‑3399537
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    
aTTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATTACAGTATAAGTGCGCTGGGt                                                                                                                                                                                                                                                                                                     >  2:396746/1‑300 (MQ=255)
          ggCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGtttt                                                                                                                                                                                                                                                                                           >  1:153448/1‑300 (MQ=255)
                            ggCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACcgcg                                                                                                                                                                                                                                                                         >  1:720287/1‑300 (MQ=255)
                             gcgcAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGa                                                                                                                                                                                                                                                                        >  2:404879/1‑300 (MQ=255)
                                              ccTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCggg                                                                                                                                                                                                                                                       >  1:705860/1‑300 (MQ=255)
                                                 gTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGaaa                                                                                                                                                                                                                                                    >  2:729511/1‑300 (MQ=255)
                                                 gTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGaaa                                                                                                                                                                                                                                                    <  1:343890/300‑1 (MQ=255)
                                                   cGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACg                                                                                                                                                                                                                                                  >  1:766622/1‑300 (MQ=255)
                                                        gTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGc                                                                                                                                                                                                                                             >  1:421441/1‑300 (MQ=255)
                                                            ctgcGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAg                                                                                                                                                                                                                                         >  1:658320/1‑300 (MQ=255)
                                                               cGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGAcc                                                                                                                                                                                                                                      <  1:14342/300‑1 (MQ=255)
                                                                    tCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAg                                                                                                                                                                                                                                 <  2:453946/300‑1 (MQ=255)
                                                                         tGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTGTTCCTGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAcc                                                                                                                                                                                                                            <  1:144789/300‑1 (MQ=255)
                                                                            gctaTCCCCGTTGCCGGTTCAGTTAACTTTGTCCCCATCGCGTCTGTCGAAGGCAATACCTGCGGCATCATCTGCATCAGCCATTCATTAGCAACACGATACACCTCTTCCGGCGTCATTGGGTTAATGCCTTTACGATCTGCTCCTCACAGCGGCAGCAGATAACGTAAACGTTGCAGCCTTGCAGTGGGACGCGCCTGAATCACGGTATAAGTGCGCTGGAGATCGAGTTTTACGGAAGAGACAGCCGCGACCAGATAGCCTTCCGGGAAACGACCGCCCAGACTGGAGGTCACCAGc                                                                                                                                                                                                                         <  2:436642/297‑1 (MQ=255)
                                                                            gCGATCCCTGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCTTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGc                                                                                                                                                                                                                         <  2:89440/300‑1 (MQ=255)
                                                                            gCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCAACAGc                                                                                                                                                                                                                         >  2:281177/1‑300 (MQ=255)
                                                                              gATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGcac                                                                                                                                                                                                                       <  2:512227/300‑1 (MQ=255)
                                                                              gATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAAATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGcac                                                                                                                                                                                                                       <  2:93674/300‑1 (MQ=255)
                                                                                    cGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAACACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGNGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTNCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCAcc                                                                                                                                                                                                                 <  2:645603/300‑1 (MQ=255)
                                                                                                 ggTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTTGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATcc                                                                                                                                                                                                    >  1:199973/1‑300 (MQ=255)
                                                                                                          gaccccATCGCGTCTGTCGAAGGCAATACCTGCGGCATCATCTGTATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCGTCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGTAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGcc                                                                                                                                                                                           <  2:19793/298‑1 (MQ=255)
                                                                                                                         ggCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCTGCAGATGCTCAAGc                                                                                                                                                                            >  2:100071/1‑300 (MQ=255)
                                                                                                                               ggCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCaaa                                                                                                                                                                      <  1:466080/300‑1 (MQ=255)
                                                                                                                                    tACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAatcatc                                                                                                                                                                 <  1:777903/300‑1 (MQ=255)
                                                                                                                                            gCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAAc                                                                                                                                                         >  1:801062/1‑300 (MQ=255)
                                                                                                                                            gCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTCCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAAc                                                                                                                                                         <  2:390377/300‑1 (MQ=255)
                                                                                                                                            gCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGANAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAAc                                                                                                                                                         >  2:716964/1‑300 (MQ=255)
                                                                                                                                                 atcTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTa                                                                                                                                                    >  1:193339/1‑300 (MQ=255)
                                                                                                                                                   cTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCTGCGTCATCGGGTGAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCAGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc                                                                                                                                                  <  2:298437/300‑1 (MQ=255)
                                                                                                                                                   cTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc                                                                                                                                                  <  2:683694/300‑1 (MQ=255)
                                                                                                                                                   cTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc                                                                                                                                                  <  1:405752/300‑1 (MQ=255)
                                                                                                                                                     gCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCCCCAACACGAATATCCGTATTCGCCGGCAGCTGCTCAAGCTGCAAATCATCAGTCCAACTGTTACCgg                                                                                                                                                >  2:436838/1‑300 (MQ=255)
                                                                                                                                                     gCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCgg                                                                                                                                                >  1:47470/1‑300 (MQ=255)
                                                                                                                                                            aCGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGAACGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAACGGCTGCAAt                                                                                                                                         >  1:707770/1‑300 (MQ=255)
                                                                                                                                                                 cATTAGNAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATNTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTAcgc                                                                                                                                    <  2:665957/300‑1 (MQ=255)
                                                                                                                                                                    tAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCNCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGa                                                                                                                                 >  1:569833/1‑300 (MQ=255)
                                                                                                                                                                         acacGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCAGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCg                                                                                                                            >  1:314031/1‑300 (MQ=255)
                                                                                                                                                                              aTGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTgcg                                                                                                                       >  1:380716/1‑300 (MQ=255)
                                                                                                                                                                               tGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTgcgc                                                                                                                      <  1:100067/300‑1 (MQ=255)
                                                                                                                                                                                 cACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCgcag                                                                                                                    >  1:669223/1‑300 (MQ=255)
                                                                                                                                                                                      ctTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAACACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAAACGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCt                                                                                                               >  1:400702/1‑300 (MQ=255)
                                                                                                                                                                                              gTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGAACGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCCACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGc                                                                                                       >  2:524620/1‑300 (MQ=255)
                                                                                                                                                                                                     ggTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGt                                                                                                >  2:467502/1‑300 (MQ=255)
                                                                                                                                                                                                             acGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCATGAATCACAGTATAAGCGCGTTGGATCTCGAGTTTTACGGAAGAGCCAACTGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTAACCAGCACATCAACAACACGAATATCCGTATTCTCCAGCCGATGCTCACGCTGCAAATCATCCGTAGAACCGTTACCGGCTGCAATTAAGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCa                                                                                        >  2:251967/2‑300 (MQ=255)
                                                                                                                                                                                                                    gATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGTACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACaaa                                                                                 <  2:276044/300‑1 (MQ=255)
                                                                                                                                                                                                                         gCCCCCCACAGCAGCAGCAGATAACGCAAATGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCTGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATcagc                                                                            <  2:432075/300‑1 (MQ=255)
                                                                                                                                                                                                                         gCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATcagc                                                                            >  1:112097/1‑300 (MQ=255)
                                                                                                                                                                                                                         gCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACATGGATTGGCAGCGCGTGGGTCGCATCACCAATcagc                                                                            >  1:227057/1‑300 (MQ=255)
                                                                                                                                                                                                                           cccccACATCAGCAGCAGATAACGCAAACGTGGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACACCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATcagcag                                                                          <  2:422125/300‑1 (MQ=255)
                                                                                                                                                                                                                              ccACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCAc                                                                       >  1:442448/1‑300 (MQ=255)
                                                                                                                                                                                                                              ccACAGCAACAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCACAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCAc                                                                       <  2:216614/300‑1 (MQ=255)
                                                                                                                                                                                                                                             aTAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGttt                                                        <  1:68690/300‑1 (MQ=255)
                                                                                                                                                                                                                                                aCGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGNAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGc                                                     <  2:712374/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCAAACTTTGCAGCCCTGCAGTCGGACGCGCCTTAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                   <  2:145948/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCAAACNTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTNGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                   <  2:656461/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCAAACGTTGCAGCCCTGCATTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGTAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGNATATCCGTATTCGCCGGCAGATGCTCNAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                   <  2:535896/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCTGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                   <  1:270221/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                   <  1:43584/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gCAAACGGTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGGATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGa                                                   <  2:321729/300‑1 (MQ=255)
                                                                                                                                                                                                                                                      aCGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAACGGCTGCAATTACGTGGATATCGTTGCGCAGCACCTGAATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGc                                               >  2:102443/1‑300 (MQ=255)
                                                                                                                                                                                                                                                       cGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGcc                                              >  1:500596/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                ccTGCAGTCGGACGCGCATGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGa                                     >  1:389238/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                   gCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCCGACCGGAAGTCACCAGCACATCAACAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAANTCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGATCGCATCACAAATCAGCAGCACGCGAATGGTCAGTTTAGCGCAGGCCACCACCTGaaca                                  >  2:200028/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                   gCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACca                                  >  1:288265/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                    cAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACcaa                                 >  1:108969/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                     aGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCAGGCAGATGCTCAAGCTGCAAATCATCCGTACAAACGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGAAGGCCAACACCTGACcaac                                >  1:136175/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                              cgcCTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCttt                       >  1:805732/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                ccTGAATCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGt                     <  1:355030/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     aTCACAGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACNCGAATGGTCAGTTTAGCGCCAGCCAACACCTGACCAACAACACCTTTNTCGCtg                >  2:673895/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                         caGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCtgatga            >  2:477934/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                         caGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCtgatga            >  1:98971/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                          aGTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGAc           <  1:487738/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           gTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATTACCAAAACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTCTAGCGACGGCCACCACATGACCAAAAACACCTTTGTCGCTGATGAcc          >  2:136874/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                           gTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGNACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGAcc          >  2:713790/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                           gTATAAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGAcc          >  1:703124/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                            tataAGCGCGCTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCg         >  1:685091/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                   gcgcTGGATATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGcc  <  1:793308/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    
ATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCGGATATCGTTGCGCAGCACCTGGATTGGCAGCGCGTGGGTCGCATCACAAATCAGCAGCACGCGACTGGTCAGTTTAGCGACGGCCACCACCTGACCAACAACACCTTTGTCGCTGATGACCGGCTGGCC  >  NC_000913/3398947‑3399537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑