Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,758,605	C→T	W419* (TGG→TGA)	selB ←	selenocysteyl‑tRNA‑specific translation elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,758,605	0	C	T	100.0%	185.4 / NA	53	W419* (TGG→TGA)	selB	selenocysteyl‑tRNA‑specific translation elongation factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

CCCGTCTCTTTTGCCAGGTCACGCACCCACCACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATAC  >  NC_000913/3758312‑3758898                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      cccGTCTCTTTTGCCAGGTCACGCACCCACCACGGTTCGTCGCCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCCATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAgcgg                                                                                                                                                                                                                                                                                                 >  2:77325/1‑300 (MQ=255)         ttttGCCAGGTCACGCACCCACCACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCNGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAAc                                                                                                                                                                                                                                                                                         <  1:544738/300‑1 (MQ=255)          tttGCCAGGTCACGCACCCACCACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAAcc                                                                                                                                                                                                                                                                                        >  1:24787/1‑300 (MQ=255)               catgttaCGCACCCATCACGGTTCGTCACCAAACATTTGCTCTACTTTTGGCCAGATGGCCTGCTGCTCTCCGCTGAAGCCCGCTTTGTGAGCTGGCAGATGCAGCCAGCCTTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCACTTCATCTTCCATTTGCAACGCCATGCGTCGCAGATGTTCGCGCCCAGGGCCAGGGTCATCGAGGTGTTGCTCATGACAAGTCACTAATGTGTCGAGAATTTTCCGCGGTCAGCGGGCGGCAACCGgcgc                                                                                                                                                                                                                                                                                   <  2:261134/294‑1 (MQ=255)                  gTCACGCACCCACCACGGTTCGTCAACAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCAtt                                                                                                                                                                                                                                                                                >  2:324306/1‑300 (MQ=255)                      cGCACCCACCACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTcaac                                                                                                                                                                                                                                                                            >  1:612124/1‑300 (MQ=255)                        cacccaccACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTcaacaa                                                                                                                                                                                                                                                                          <  1:363965/300‑1 (MQ=255)                          cccaccACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGc                                                                                                                                                                                                                                                                        >  1:485882/1‑300 (MQ=255)                          cccaccACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTCCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGc                                                                                                                                                                                                                                                                        >  2:433688/1‑300 (MQ=255)                            caccacGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGTCGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGCTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTAGAGAATTTTCCGCTGTCAGCGGGCGGCAAACGGCGCATTCAACAAGCta                                                                                                                                                                                                                                                                      >  2:226739/1‑300 (MQ=255)                              ccacGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCtata                                                                                                                                                                                                                                                                    >  1:779138/1‑300 (MQ=255)                                acGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATNTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAAc                                                                                                                                                                                                                                                                  <  1:564977/300‑1 (MQ=255)                                 cGGTTCGTCACCAAACATTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAAcc                                                                                                                                                                                                                                                                 <  2:341217/300‑1 (MQ=255)                                 cGGTTCGTCACCAAACATTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAAcc                                                                                                                                                                                                                                                                 <  1:774568/300‑1 (MQ=255)                                        tCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATNTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCNGCCTGa                                                                                                                                                                                                                                                          >  2:671268/1‑300 (MQ=255)                                        tCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTCTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGTAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCGGCGGGCGGCAACCGGCGCATTCAACAAGCTATCACCAGCCTGa                                                                                                                                                                                                                                                          >  2:328662/1‑300 (MQ=255)                                               atagttgCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCTCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTTTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCTCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCGGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAAc                                                                                                                                                                                                                                                   <  2:93452/294‑1 (MQ=255)                                                cAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAAcc                                                                                                                                                                                                                                                  <  1:30358/300‑1 (MQ=255)                                                          gCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTAAATAAGCAACAGTACCANCGCTTCATCTTCCATTGGCAACGCCANACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTAGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCGATAACCAGCCTGAATATAACCAGGCTGTTgc                                                                                                                                                                                                                                        >  2:558435/1‑300 (MQ=255)                                                              tttGCCAGATGGCCTTCTGCCCGTCGCTGAAGCCCGTTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTTTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCTGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTgcagca                                                                                                                                                                                                                                    <  2:110976/300‑1 (MQ=255)                                                                                gctCTTCGCTTAGGCCCACTTTTTGGTCTTGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCAGTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCTGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGc                                                                                                                                                                                                                  <  2:267779/300‑1 (MQ=255)                                                                                gctCTTCGCTGAAGCCCGCGTTGTGGTCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTATCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGGCGTTCGCGCCCGGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGc                                                                                                                                                                                                                  <  2:86190/300‑1 (MQ=255)                                                                                     tCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTg                                                                                                                                                                                                             >  1:292283/1‑300 (MQ=255)                                                                                                        gATCTGGCAGGTGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAAAAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGa                                                                                                                                                                                          >  2:465260/1‑300 (MQ=255)                                                                                                                 gATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGAGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCaa                                                                                                                                                                                 >  1:329378/1‑300 (MQ=255)                                                                                                                     cagccagcCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGtt                                                                                                                                                                             >  1:304433/1‑300 (MQ=255)                                                                                                                         cagccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTGTGCAATCCGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGCCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGNTAATGTGTCGAGAATTTTCCGCTGTCNGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAAcc                                                                                                                                                                         <  2:537687/300‑1 (MQ=255)                                                                                                                          agccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAAccg                                                                                                                                                                        <  2:618234/300‑1 (MQ=255)                                                                                                                           gccGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCNCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAAccgc                                                                                                                                                                       >  1:577509/1‑300 (MQ=255)                                                                                                                             cGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAAccgcgc                                                                                                                                                                     >  1:327892/1‑300 (MQ=255)                                                                                                                                   ggCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGtt                                                                                                                                                               >  2:525910/1‑300 (MQ=255)                                                                                                                                    gCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTc                                                                                                                                                              >  1:525605/1‑300 (MQ=255)                                                                                                                                               cgccgcTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAg                                                                                                                                                   <  1:443296/300‑1 (MQ=255)                                                                                                                                                 ccgcTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGAt                                                                                                                                                 <  1:97485/300‑1 (MQ=255)                                                                                                                                                              tCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGNATTTTCCGCTGTCAGCGGGCGGCAACNGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACgcatcggcat                                                                                                                                    >  2:530885/1‑300 (MQ=255)                                                                                                                                                                   tCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGTGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCTGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGctc                                                                                                                               <  2:363558/300‑1 (MQ=255)                                                                                                                                                                         aGCAACAGTACCATCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCATGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGcc                                                                                                                         <  2:325363/300‑1 (MQ=255)                                                                                                                                                                                   ccAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGGTAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGNCGCATTCAACAAGCTATAACCAGCCTGAATATAACCATGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAagag                                                                                                               <  2:184698/300‑1 (MQ=255)                                                                                                                                                                                                             gCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGc                                                                                     >  1:432593/1‑300 (MQ=255)                                                                                                                                                                                                                     tCGCAGACGTTCGCGCCCAGGGCCAAATTCATCGCAATGTTGCTCATGATAAGTCGCTAATGTGTAGAGAGTTTTCCGCTGTCAGCGGGCGGCAACCGGCTCATTCAACAAGCTATAACCAGCCTGAATATACCCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCAGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAAAGCATAGGCATCGCTCTGCGCACATGCACGAGACGCAAGCCGCTGCCGATATTCCGGCGTACGttt                                                                             >  2:375050/1‑300 (MQ=255)                                                                                                                                                                                                                          aaCGTTCGCGCCCAGTGCCAGGTTCATCTCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACcgc                                                                        <  2:523568/299‑1 (MQ=255)                                                                                                                                                                                                                              ttCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGAcg                                                                    >  1:314629/1‑300 (MQ=255)                                                                                                                                                                                                                                    cccAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGGGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGcggcgg                                                              >  2:475652/1‑300 (MQ=255)                                                                                                                                                                                                                                        gggCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGTAAGAGACGCCAGCCATTGCAGATATTCCGGCTCACGTTTACCGCGACGCGGCGGGTTa                                                          >  2:90687/1‑300 (MQ=255)                                                                                                                                                                                                                                          gCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAg                                                        >  1:539101/1‑300 (MQ=255)                                                                                                                                                                                                                                           ccAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCNGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGGTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAATAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGc                                                       <  2:609833/300‑1 (MQ=255)                                                                                                                                                                                                                                            cAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCa                                                      <  2:691623/300‑1 (MQ=255)                                                                                                                                                                                                                                            cAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCa                                                      <  1:42240/300‑1 (MQ=255)                                                                                                                                                                                                                                                ttCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCac                                                  <  1:321530/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCGCTAATGTGTCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGc                        >  1:780463/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gtCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACACGCGCTCCGGCCAGCGTGTTGTGGGCAGAGat              >  2:157671/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gtCGAGAATTTTCCGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAAGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGTCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAATAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGat              <  2:379671/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                ccGCTGTCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATAc  <  2:401335/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      CCCGTCTCTTTTGCCAGGTCACGCACCCACCACGGTTCGTCACCAAACAGTGGCTCTGCTTTTTGCCAGATGGCCTGCTGCTCTTCGCTGAAGCCCGCTTTGTGATCTGGCAGATGCAGCCAGCCGTGATGGCTGTGGATGTCGCCGCTTTCGCGCATCTTTTCAATCAGCAACAGTACCAGCGCTTCATCTTCCATTGGCAACGCCATACGTCGCAGACGTTCGCGCCCAGGGCCAGGTTCATCGCGATGTTGCTCATGATAAGTCGCTAATGTGTCGAGAATTTTCCGCTGCCAGCGGGCGGCAACCGGCGCATTCAACAAGCTATAACCAGCCTGAATATAACCAGGCTGTTGCAGCAATTCGCGCATCCCTTCGCCGTTGAGCTGGCGCGCCCAGGCGAAATCCGCAAGGTTAACCGCGCCGCGTTCCAGATGAACAGATAACGCATCGGCATCGCTCTGCGCCCGTGCAAGAGACGCCAGCCATTGCAGATATTCCGGCTTACGTTTACCGCGACGCGGCGGGTTAAGCATCACGACGCGCGCTCCGGCCAGCGTGTTGCGGGCAGAGATATCGCGCAATAC  >  NC_000913/3758312‑3758898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑