breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 38,950 G→A P56S (CCG→TCG)  caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
RA 128,115 G→A A68A (GCG→GCA lpd → lipoamide dehydrogenase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 283,362 G→T W54C (TGG→TGT yagF → D‑xylonate dehydratase
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 305,486 G→A F444F (TTC→TTT ecpD ← fimbrial adhesin EcpD
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 314,109 G→A intergenic (‑867/‑1182) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 315,439 G→A R50H (CGC→CAC)  insE1 → IS3 element protein InsE
RA 317,019 G→A intergenic (+566/+707) insF1 → / ← rclC IS3 element protein InsF/DUF417 domain‑containing inner membrane protein RclC
RA 320,976 G→A E250E (GAG→GAA rclR → DNA‑binding transcriptional activator RclR
RA 334,699 G→A F108F (TTC→TTT yahC ← uncharacterized protein YahC
RA 335,395 G→A G39E (GGG→GAG)  yahD → ankyrin repeat‑containing protein YahD
RA 338,327 G→A M1M (ATG→ATA) † yahG → DUF1116 domain‑containing protein YahG
RA 338,415 G→A V31M (GTG→ATG)  yahG → DUF1116 domain‑containing protein YahG
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 340,333 G→A V57I (GTC→ATC)  yahI → carbamate kinase‑like protein YahI
RA 351,695 G→A E161K (GAA→AAA)  prpD → 2‑methylcitrate dehydratase
RA 352,701 G→A intergenic (+35/‑5) prpD → / → prpE 2‑methylcitrate dehydratase/propionyl‑CoA synthetase
RA 353,604 G→A G300E (GGG→GAG)  prpE → propionyl‑CoA synthetase
RA 355,256 G→A G112D (GGT→GAT)  codB → cytosine transporter
RA 356,109 G→A L396L (CTG→CTA codB → cytosine transporter
RA 361,896 G→A intergenic (‑36/+30) lacA ← / ← lacY galactoside O‑acetyltransferase/lactose permease
RA 363,407 G→A Q967* (CAA→TAA)  lacZ ← beta‑galactosidase
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 488,122 G→A A529A (GCG→GCA mscK → potassium dependent, small conductance mechanosensitive channel
RA 488,151 G→A G539D (GGT→GAT)  mscK → potassium dependent, small conductance mechanosensitive channel
RA 488,699 G→A A722T (GCA→ACA)  mscK → potassium dependent, small conductance mechanosensitive channel
RA 509,517 C→T E621E (GAG→GAA copA ← Cu(+) exporting P‑type ATPase
RA 544,861 C→G L144L (CTG→CTC allC ← allantoate amidohydrolase
RA 587,661 G→A S107S (TCC→TCT ybcH ← putative exopolysaccharide secretion system auxiliary component
JC JC 607,765 IS150 (+) +3 bp noncoding (26‑28/60 nt) sokE ← small RNA SokE
MC JC 679,777 Δ8,074 bp IS5‑mediated [ybeU]gltI [ybeU], djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
JC JC 729,860 IS150 (–) +3 bp coding (278‑280/4194 nt) rhsC → rhs element protein RhsC
RA 806,081 G→A L401L (CTG→TTG)  ybhC ← outer membrane lipoprotein YbhC
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 855,674 G→A A24V (GCG→GTG)  ldtB ← L,D‑transpeptidase LdtB
RA 863,304 G→A L70L (CTG→TTG)  ybiY ← putative pyruvate formate‑lyase activating enzyme YbiY
RA 869,418 G→A R3K (AGA→AAA)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 871,045 G→A V27I (GTC→ATC)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 873,115 G→A R46H (CGC→CAC)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 884,344 G→A G224G (GGG→GGA mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 897,642 G→A V187I (GTC→ATC)  potI → putrescine ABC transporter membrane subunit PotI
RA 909,012 G→A L95L (CTG→TTG)  ltaE ← low‑specificity L‑threonine aldolase
JC JC 927,393 IS2 (–) +5 bp coding (36‑40/705 nt) aat ← leucyl/phenylalanyl‑tRNA‑‑protein transferase
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
JC JC 972,851 IS2 (–) +5 bp coding (547‑551/780 nt) elyC ← envelope biogenesis factor
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,070,014 A→G L114P (CTG→CCG)  rutF ← FMN reductase RutF
RA 1,078,342 G→T R181S (CGC→AGC)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,150,616 C→T L297L (CTG→TTG)  fabD → [acyl‑carrier‑protein] S‑malonyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
JC JC 1,186,598 IS150 (–) +3 bp coding (755‑757/1227 nt) pepT → peptidase T
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,234,887 IS2 (+) +5 bp intergenic (‑170/‑47) nhaB ← / → fadR Na(+):H(+) antiporter NhaB/DNA‑binding transcriptional dual regulator FadR
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
RA 1,276,230 C→T P463P (CCG→CCA narX ← sensor histidine kinase NarX
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,234 T→G L18R (CTA→CGA)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,315,384 G→A Y129Y (TAC→TAT yciF ← DUF892 domain‑containing protein YciF
RA 1,317,129 G→C Q32E (CAG→GAG)  trpA ← tryptophan synthase subunit alpha
RA 1,322,325 G→A L208F (CTC→TTC)  trpE ← anthranilate synthase subunit TrpE
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,356,932 G→A T60I (ACC→ATC)  sapA ← putative periplasmic binding protein SapA
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,366,433 G→A G295D (GGC→GAC)  puuE → 4‑aminobutyrate aminotransferase PuuE
MC JC 1,381,790 Δ14,254 bp IS5‑mediated ompGynaI ompG, ycjW, ycjX, ycjF, tyrR, tpx, ycjG, mpaA, ymjC, ymjD, ycjY, pgrR, mppA, ynaI
RA 1,400,978 C→T G266S (GGT→AGT)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter
JC JC 1,461,938 IS150 (–) +4 bp coding (1046‑1049/1206 nt) paaJ → beta‑ketoadipyl‑CoA thiolase
JC JC 1,492,142 IS150 (–) +3 bp noncoding (24‑26/57 nt) sokB → putative small regulatory RNA antitoxin SokB
RA 1,501,192 C→T P207L (CCT→CTT)  tehA → tellurite resistance protein
RA 1,510,161 G→A Q53Q (CAG→CAA ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,514,172 C→T R114R (CGC→CGT ydcU → putative ABC transporter membrane subunit YdcU
RA 1,517,461 C→T L25F (CTT→TTT)  ortT → orphan toxin OrtT
RA 1,531,755 A→G intergenic (‑1251/‑61) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
JC JC 1,579,695 IS5 (+) +4 bp coding (1093‑1096/1158 nt) ydeM ← putative anaerobic sulfatase maturation enzyme YdeM
RA 1,603,321 C→T L101L (CTC→CTT lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,604,223 C→T G59G (GGC→GGT lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,604,840 C→T S265F (TCC→TTC)  lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,605,533 C→T S161S (AGC→AGT lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,606,464 C→T A122V (GCC→GTC)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,608,089 C→T A248A (GCC→GCT tam → trans‑aconitate 2‑methyltransferase
RA 1,610,381 A→G S100S (AGT→AGC uxaB ← tagaturonate reductase
RA 1,632,034 C→T Q84Q (CAG→CAA ydfJ ← putative transporter YdfJ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 1,656,194 IS2 (–) +5 bp coding (11‑15/561 nt) speG → spermidine N‑acetyltransferase
RA 1,695,900 C→T A58T (GCA→ACA)  uidA ← beta‑glucuronidase
RA 1,701,372 C→T A139V (GCT→GTT)  malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,734,167 G→A intergenic (‑66/‑268) grxD ← / → mepH glutaredoxin 4/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH
JC JC 1,743,375 IS1 (+) +8 bp intergenic (‑133/‑75) ribC ← / → mdtK riboflavin synthase/multidrug efflux pump MdtK
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,783,681 G→A E235E (GAG→GAA fadK → short chain acyl‑CoA synthetase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
JC JC 1,907,451 IS2 (+) +5 bp coding (137‑141/144 nt) yobF ← DUF2527 domain‑containing protein YobF
RA 1,963,862 C→T E397K (GAG→AAG)  flhA ← flagellar biosynthesis protein FlhA
RA 1,963,973 C→T E360K (GAA→AAA)  flhA ← flagellar biosynthesis protein FlhA
RA 1,964,853 C→T Q66Q (CAG→CAA flhA ← flagellar biosynthesis protein FlhA
RA 1,974,896 C→T V143V (GTG→GTA
V143V (GTG→GTA
cheA ←
cheA ←
chemotaxis protein CheA
chemotaxis protein CheA
RA 1,975,279 C→T A16T (GCG→ACG)  cheA ← chemotaxis protein CheA
RA 1,977,100 C→T V14I (GTT→ATT)  motA ← motility protein A
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 1,987,988 G→A intergenic (‑29/‑234) azuC ← / → yecR uncharacterized protein AzuC/lipoprotein YecR
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
JC 2,041,058 (TATAAG)1→2 coding (576/636 nt) msrQ → membrane‑bound flavocytochrome MsrQ
RA 2,072,723 C→T V395V (GTC→GTT flu → self recognizing antigen 43 (Ag43) autotransporter
RA 2,074,441 G→A S968N (AGC→AAC)  flu → self recognizing antigen 43 (Ag43) autotransporter
RA 2,076,399 G→A R31H (CGT→CAT)  yeeS → RadC‑like JAB domain‑containing protein YeeS
RA 2,076,909 G→A W31* (TGG→TGA yeeT → DUF987 domain‑containing protein YeeT
RA 2,077,731 G→A A55T (GCA→ACA)  cbtA → cytoskeleton‑binding toxin CbtA
RA 2,103,351 G→A pseudogene (39/446 nt) wbbL ← interrupted rhamnosyltransferase WbbL
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,188,711 A→G Y734H (TAT→CAT)  yehB ← putative fimbrial usher protein YehB
RA 2,195,035 G→A A246T (GCG→ACG)  metG → methionine‑‑tRNA ligase
RA 2,216,871 G→A R179R (CGC→CGT yehX ← glycine betaine ABC transporter ATP binding subunit YehX
RA 2,219,653 G→A intergenic (‑172/+39) osmF ← / ← bglX glycine betaine ABC transporter periplasmic binding protein OsmF/beta‑D‑glucoside glucohydrolase, periplasmic
RA 2,248,076 C→T G153S (GGT→AGT)  lysP ← lysine:H(+) symporter
RA 2,264,483 G→A T207T (ACG→ACA setB → sugar exporter SetB
RA 2,264,637 G→A G259R (GGG→AGG)  setB → sugar exporter SetB
RA 2,284,395 G→C R7P (CGC→CCC)  lapC → lipopolysaccharide signal transducer LapC
RA 2,294,130 G→A L259L (CTG→TTG)  ccmF ← holocytochrome c synthase CcmF component
RA 2,311,176 G→A A120V (GCG→GTG)  ftp ← FAD:protein FMN transferase
RA 2,316,917 G→A intergenic (+90/+110) rcsB → / ← rcsC DNA‑binding transcriptional activator RcsB/histidine kinase RcsC
JC JC 2,319,848 IS2 (+) +5 bp coding (25‑29/2850 nt) rcsC ← histidine kinase RcsC
JC JC 2,320,126 IS2 (–) +5 bp coding (84‑88/1827 nt) atoS → sensor histidine kinase AtoS
RA 2,320,839 T→C L266P (CTG→CCG)  atoS → sensor histidine kinase AtoS
RA 2,358,366 G→A N160N (AAC→AAT yfaU ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
RA 2,361,166 T→A T83T (ACA→ACT rhmD ← L‑rhamnonate dehydratase
RA 2,382,718 G→A M2I (ATG→ATA elaD → protease ElaD
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,386,800 G→A G314D (GGT→GAT)  yfbL → putative peptidase YfbL
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,417,231 G→A D51N (GAT→AAT)  yfcC → putative transporter YfcC
RA 2,428,433 G→A A65V (GCG→GTG)  ubiX ← flavin prenyltransferase
RA 2,432,054 G→A P297S (CCA→TCA)  folC ← bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
RA 2,446,640 G→T G278G (GGC→GGA aroC ← chorismate synthase
RA 2,454,633 G→A intergenic (‑2297/+450) yfcS ← / ← yfcV putative fimbrial chaperone YfcS/putative fimbrial protein YfcV
RA 2,463,652 G→A R214H (CGT→CAT)  yfdF → protein YfdF
RA 2,466,572 G→A A10T (GCA→ACA)  intS → prophage CPS‑53 integrase
RA 2,482,646 G→A P232S (CCC→TCC)  emrK ← tripartite efflux pump membrane fusion protein EmrK
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,506,876 G→A T340I (ACC→ATC)  fryC ← putative PTS enzyme IIC component FryC
RA 2,551,581 G→A A49V (GCG→GTG)  yfeZ ← putative inner membrane protein
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,698,402 IS30 (+) +2 bp coding (148‑149/636 nt) pgpC ← phosphatidylglycerophosphatase C
RA 2,707,579 G→T L75I (CTT→ATT)  rseC ← protein RseC
JC JC 2,771,871 IS2 (–) +5 bp coding (280‑284/315 nt) yfjU ← putative arsenate reductase‑like protein YfjU
RA 2,803,048 G→T A567A (GCG→GCT nrdE → ribonucleoside‑diphosphate reductase 2 subunit alpha
RA 2,894,892 C→T intergenic (+121/+27) ygcP → / ← ygcQ putative anti‑terminator regulatory protein/putative flavoprotein
RA 2,895,577 C→T G68D (GGC→GAC)  ygcQ ← putative flavoprotein
RA 2,895,651 C→T Q43Q (CAG→CAA ygcQ ← putative flavoprotein
RA 2,896,161 C→T G132E (GGG→GAG)  ygcR ← putative flavoprotein
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
MC JC 3,102,311 Δ27,835 bp IS5‑mediated [trmB][yghO] 24 genes
RA 3,144,041 C→T V42I (GTC→ATC)  hybB ← hydrogenase 2 membrane subunit
RA 3,171,795 G→T A438S (GCG→TCG)  qseC → sensor histidine kinase QseC
RA 3,243,482 G→A T420T (ACC→ACT uxaC ← D‑glucoronate/D‑galacturonate isomerase
RA 3,247,141 G→A E164K (GAA→AAA)  exuR → DNA‑binding transcriptional repressor ExuR
RA 3,272,462 G→A A97V (GCA→GTA)  garR ← tartronate semialdehyde reductase
RA 3,276,325 G→A E348E (GAG→GAA garD → GarD
RA 3,279,461 A→G H183R (CAT→CGT)  kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,293,673 G→A E92K (GAA→AAA)  lpoA → outer membrane lipoprotein ‑ activator of MrcA activity
RA 3,302,892 G→A W135* (TGG→TAG)  ubiV → ubiquinone biosynthesis protein UbiV
RA 3,310,463 C→A E236* (GAG→TAG)  pnp ← polynucleotide phosphorylase
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,342,672 G→A E134K (GAA→AAA)  kdsC → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase KdsC
RA 3,342,825 G→A G185R (GGG→AGG)  kdsC → 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase KdsC
RA 3,360,043 C→A F282L (TTC→TTA gltD → glutamate synthase subunit GltD
MC JC 3,362,328 Δ3,228 bp IS5‑mediated [yhcA][yhcE] [yhcA], yhcD, [yhcE]
RA 3,377,372 G→A P17S (CCT→TCT)  sspA ← stringent starvation protein A
RA 3,377,902 G→A G102G (GGC→GGT rpsI ← 30S ribosomal subunit protein S9
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,388,421 C→T A236T (GCC→ACC)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,389,971 C→T S151F (TCC→TTC)  aaeR → DNA‑binding transcriptional activator AaeR
RA 3,390,186 C→T P223S (CCG→TCG)  aaeR → DNA‑binding transcriptional activator AaeR
RA 3,392,825 G→A T1145I (ACT→ATT)  yhdP ← phospholipid transport factor YhdP
RA 3,397,720 C→T E26K (GAA→AAA)  rng ← RNase G
RA 3,413,728 G→A intergenic (‑263/‑136) envR ← / → acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,476,285 G→A H52H (CAC→CAT yheO ← DNA‑binding transcriptional regulator YheO
RA 3,515,325 G→A H197Y (CAC→TAC)  dam ← DNA adenine methyltransferase
RA 3,518,683 G→A P128S (CCG→TCG)  aroK ← shikimate kinase 1
RA 3,522,059 C→T E231E (GAG→GAA hofM ← DNA utilization protein HofM
RA 3,539,633 G→A G750D (GGT→GAT)  yhgF → putative RNA‑binding protein YhgF
RA 3,539,686 G→A A768T (GCA→ACA)  yhgF → putative RNA‑binding protein YhgF
RA 3,540,703 G→A E99E (GAG→GAA feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,653,231 Δ52,001 bp IS5‑mediated yhiS[dppB] 43 genes
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
MC JC 3,708,467 Δ168 bp [proK] [proK]
MC JC 3,722,076 Δ15 bp IS150‑mediated insK → / ← glyS IS150 family conserved protein InsB/glycine‑‑tRNA ligase subunit beta
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,781,198 C→A intergenic (+181/‑17) lldD → / → trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,820,207 C→T G322S (GGT→AGT)  ligB ← DNA ligase B
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,825,407 C→T G66G (GGC→GGT recG → ATP‑dependent DNA helicase RecG
RA 3,825,900 G→A A231T (GCG→ACG)  recG → ATP‑dependent DNA helicase RecG
RA 3,828,778 C→A intergenic (‑113/‑167) gltS ← / → xanP glutamate:sodium symporter/xanthine:H(+) symporter XanP
RA 3,834,118 C→T V140V (GTG→GTA yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,838,186 C→T intergenic (+49/‑62) setC → / → yicL putative arabinose exporter/putative transporter YicL
RA 3,839,040 C→T L265L (CTG→TTG)  yicL → putative transporter YicL
RA 3,839,178 C→T W272* (TGG→TGA nlpA ← lipoprotein‑28
RA 3,840,776 C→T V322I (GTT→ATT)  nepI ← purine ribonucleoside exporter
RA 3,847,493 C→T A378T (GCA→ACA)  uhpC ← inner membrane protein sensing glucose‑6‑phosphate
RA 3,849,109 C→T S343N (AGC→AAC)  uhpB ← sensory histidine kinase UhpB
RA 3,849,393 C→T W248* (TGG→TGA uhpB ← sensory histidine kinase UhpB
RA 3,858,628 C→T D497N (GAC→AAC)  yidK ← putative transporter YidK
RA 3,863,026 C→T G193D (GGC→GAC)  glvC ← putative PTS enzyme II component GlvC
RA 3,864,292 C→T P132S (CCG→TCG)  yidP → putative DNA‑binding transcriptional regulator YidP
RA 3,865,138 C→T G379E (GGG→GAG)  yidE ← putative transport protein YidE
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,873,018 C→T A199T (GCG→ACG)  dgoA ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA 3,876,009 C→T S191F (TCC→TTC)  yidX → putative lipoprotein YidX
RA 3,876,361 C→T G198S (GGT→AGT)  yidA ← sugar phosphatase YidA
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,880,995 C→T G76D (GGC→GAC)  recF ← recombination mediator protein RecF
RA 3,883,568 C→T K54K (AAG→AAA dnaA ← chromosomal replication initiator protein DnaA
RA 3,890,215 C→T intergenic (+70/‑21) tnaA → / → tnaB tryptophanase/tryptophan:H(+) symporter TnaB
JC JC 3,890,222 IS5 (–) +4 bp intergenic (+77/‑11) tnaA → / → tnaB tryptophanase/tryptophan:H(+) symporter TnaB
RA 3,894,704 C→T S18L (TCA→TTA)  yieF → quinone reductase
RA 3,902,608 C→T G365D (GGC→GAC)  bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,904,791 C→T W269* (TGG→TGA bglF ← beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
RA 3,911,468 C→T A20T (GCT→ACT)  pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,917,210 C→T A56T (GCA→ACA)  atpD ← ATP synthase F1 complex subunit beta
RA 3,919,605 C→T G85S (GGC→AGC)  atpA ← ATP synthase F1 complex subunit alpha
RA 3,920,316 C→T D30N (GAC→AAC)  atpH ← ATP synthase F1 complex subunit delta
RA 3,933,793 C→T Q6* (CAG→TAG)  rbsA → ribose ABC transporter ATP binding subunit
RA 3,935,212 C→T R479C (CGT→TGT)  rbsA → ribose ABC transporter ATP binding subunit
RA 3,937,813 C→T P174S (CCT→TCT)  rbsK → ribokinase
RA 3,938,959 G→A G245R (GGA→AGA)  rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,943,540 C→T intergenic (+30/‑164) gltU → / → rrlC tRNA‑Glu/23S ribosomal RNA
RA 3,943,585 G→T intergenic (+75/‑119) gltU → / → rrlC tRNA‑Glu/23S ribosomal RNA
RA 3,952,987 C→T S168S (TCC→TCT ilvE → branched‑chain‑amino‑acid aminotransferase
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,008,757 C→A S101S (TCG→TCT rhtB ← L‑homoserine/L‑homoserine lactone/L‑threonine exporter
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,099,498 G→T A343E (GCG→GAG)  rhaT ← rhamnose/lyxose:H(+) symporter
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,159,493 C→T G433D (GGC→GAC)  sthA ← soluble pyridine nucleotide transhydrogenase
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,178,371 G→T intergenic (+83/‑76) nusG → / → rplK transcription termination/antitermination factor NusG/50S ribosomal subunit protein L11
RA 4,184,612 G→A G1123D (GGT→GAT)  rpoB → RNA polymerase subunit beta
JC JC 4,197,558 IS2 (+) +5 bp coding (656‑660/774 nt) nudC → RNA decapping hydrolase
RA 4,199,747 G→A A82T (GCC→ACC)  yjaG → DUF416 domain‑containing protein YjaG
RA 4,200,155 (C)6→5 intergenic (+61/‑126) yjaG → / → hupA DUF416 domain‑containing protein YjaG/DNA‑binding protein HU‑alpha
RA 4,205,310 G→A P208S (CCG→TCG)  purD ← phosphoribosylamine‑‑glycine ligase
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,217,172 G→A G22S (GGC→AGC)  aceA → isocitrate lyase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,236,551 G→A G97D (GGC→GAC)  yjbF → lipoprotein YjbF
RA 4,238,103 G→A G157D (GGC→GAC)  yjbH → putative lipoprotein YjbH
RA 4,251,266 G→A intergenic (+792/‑1240) malM → / → ubiC maltose regulon periplasmic protein/chorismate lyase
RA 4,255,284 G→A L395L (CTG→TTG)  plsB ← glycerol‑3‑phosphate 1‑O‑acyltransferase
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,351,032 C→A G211C (GGC→TGC)  dcuS ← sensor histidine kinase DcuS
JC JC 4,366,849 IS4 (+) +12 bp noncoding (92‑103/113 nt) aspX ← small regulatory RNA AspX
RA 4,375,455 G→T P68Q (CCG→CAG)  epmB ← lysine 2,3‑aminomutase
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,429,819 G→A intergenic (+264/‑45) fklB → / → cycA peptidyl‑prolyl cis‑trans isomerase FklB/D‑serine/alanine/glycine/:H(+)symporter
RA 4,430,212 G→A A117T (GCA→ACA)  cycA → D‑serine/alanine/glycine/:H(+)symporter
RA 4,462,413 T→C E83G (GAG→GGG)  nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,542,517 G→T G161W (GGG→TGG)  fimE → regulator for fimA
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196304 1211462 15159 36 [33] [34] 36 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1266030 1266756 727 36 [34] [33] 35 [prfA]–[prmC] [prfA],[prmC]
* * ÷ NC_000913 1978495 1979270–1978503 9–776 112 [0] [0] 130 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2112201 2174816 62616 100 [0] [0] 84 [rfbB]–[gatA] [rfbB],galF,wcaM,wcaL,wcaK,wzxC,wcaJ,cpsG,cpsB,wcaI,gmm,fcl,gmd,wcaF,wcaE,wcaD,wcaC,wcaB,wcaA,wzc,wzb,wza,yegH,asmA,dcd,udk,dgcE,alkA,yegD,yegI,yegJ,pphC,yegL,sibA,ibsA,sibB,ibsB,mdtA,mdtB,mdtC,mdtD,baeS,baeR,yegP,trhP,cyaR,ogrK,yegR,yegS,gatR,insE5,insF5,gatR,gatD,gatB,[gatA]
* * ÷ NC_000913 3423857–3424233 3424446–3424239 7–590 35 [34] [33] 35 [rrlD] [rrlD]
* * ÷ NC_000913 3625589 3639754 14166 115 [0] [2] 163 yhhJ–uspB yhhJ,rbbA,yhiI,yhiY,yhiJ,yhiM,yhiN,pitA,uspB
* * ÷ NC_000913 4134095 4152952 18858 120 [0] [0] 138 [katG]–[ppc] [katG],yijE,yijF,gldA,fsaB,ptsA,frwC,frwB,pflD,pflC,frwD,yijO,eptC,[ppc]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)112 (1.070) 72/598 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1978503 =NA (NA)82 (0.790) 57/598 0.5 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 2174817 = 0 (0.000)coding (233/453 nt) gatA galactitol‑specific PTS enzyme IIA component
* ? NC_000913 1979271 =0 (0.000)130 (1.250) 86/598 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 21122000 (0.000)100 (0.960) 68/598 0.2 100% coding (862/1086 nt) rfbB dTDP‑glucose 4,6‑dehydratase 1
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? NC_000913 = 36255880 (0.000)112 (1.080)
+CCC
74/592 0.1 100% intergenic (+74/+91) yhhI/yhhJ putative transposase/ABC transporter family protein YhhJ
?NC_000913 3720633 = NA (NA)noncoding (1/1443 nt) IS150 repeat region
* ? NC_000913 3639755 =0 (0.000)160 (1.540) 89/594 0.0 100% intergenic (‑35/‑356) uspB/uspA universal stress protein B/universal stress protein A
?NC_000913 = 3722075 NA (NA)noncoding (1443/1443 nt) IS150 repeat region
* ? NC_000913 3720633 =NA (NA)138 (1.340) 87/592 0.0 100% noncoding (1/1443 nt) IS150 repeat region
?NC_000913 4152953 = 0 (0.000)coding (146/2652 nt) ppc phosphoenolpyruvate carboxylase
* ? NC_000913 = 3722075NA (NA)119 (1.140) 79/598 0.1 100% noncoding (1443/1443 nt) IS150 repeat region
?NC_000913 = 4134094 0 (0.000)coding (260/2181 nt) katG catalase/hydroperoxidase HPI
* ? NC_000913 4542682 =55 (0.530)116 (1.150) 64/580 0.2 69.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 49 (0.480)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269064 (0.610)109 (1.080) 76/580 0.1 66.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 49 (0.480)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit