Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	488,699	G→A	A722T (GCA→ACA)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	488,699	0	G	A	100.0%	285.3 / NA	77	A722T (GCA→ACA)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/41);  total (36/41)

CCAGCCACTGGCGTCGGCAAATTGTCCGCATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTCAGC  >  NC_000913/488403‑488989                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   ccAGCCACTGGCGTCGGCAAATTGTCCGCATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCAACATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGTTACAGGCTACTTCTACACTACGCTGCATCTGACAg                                                                                                                                                                                                                                                                                                 >  2:111903/1‑300 (MQ=255)                   aaaTTGTCCGCATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCATTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCg                                                                                                                                                                                                                                                                               >  1:705932/1‑300 (MQ=255)                        gTCCGCATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACCGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTAt                                                                                                                                                                                                                                                                          >  2:109638/1‑300 (MQ=255)                             cATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGt                                                                                                                                                                                                                                                                     >  1:268609/1‑300 (MQ=255)                             cATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCAGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGt                                                                                                                                                                                                                                                                     >  2:14405/1‑300 (MQ=255)                                 aGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGatc                                                                                                                                                                                                                                                                 >  2:689325/1‑300 (MQ=255)                                    ctcGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGatcatc                                                                                                                                                                                                                                                              <  1:614286/300‑1 (MQ=255)                                          ttGCTGCCTATCCATTTCTGGTTTGTGGTGGCAGAACTTGCCCCGTTGCATCTGATGGATGATGTGCTGGGGCAATCGATGATTTTCTTCAACCGGCTGCTGATTGCCTTCCTGGTATGTCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAAc                                                                                                                                                                                                                                                        <  2:72586/300‑1 (MQ=255)                                           tgctgcCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAAcc                                                                                                                                                                                                                                                       <  1:506782/300‑1 (MQ=255)                                                   aTCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTNACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTNCTGTAc                                                                                                                                                                                                                                               >  2:656421/1‑300 (MQ=255)                                                   aTCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTAc                                                                                                                                                                                                                                               <  1:119573/300‑1 (MQ=255)                                                    tCCATTTCTAGCCTGTTGTTGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCTATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTAcc                                                                                                                                                                                                                                              <  2:55200/300‑1 (MQ=255)                                                        tttCTGTTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGTGCAATCGATGATTTTCTTCAACCTGCTGCCGATTGCCTTCCTGTTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGAc                                                                                                                                                                                                                                          <  2:779605/300‑1 (MQ=255)                                                        tttCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAATCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGNTGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGAGAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGAc                                                                                                                                                                                                                                          <  1:556056/300‑1 (MQ=255)                                                        tttCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGNTGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGAc                                                                                                                                                                                                                                          <  1:561789/300‑1 (MQ=255)                                                             ggtcgggagtggCAGAACTTTCCCCGTTGCACCGGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGCATGGCCAATGTGCCTCGAAAGCTGGCGTGAGAAAGNGTCGCACACCATGCGATTGGTCACCGTTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTTCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTAc                                                                                                                                                                                                                                     <  2:181217/292‑1 (MQ=255)                                                             ggTCTTTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCGGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTAc                                                                                                                                                                                                                                     <  2:520901/300‑1 (MQ=255)                                                             ggTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTAc                                                                                                                                                                                                                                     <  1:767997/300‑1 (MQ=255)                                                                  gtggtgGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGt                                                                                                                                                                                                                                <  1:775443/300‑1 (MQ=255)                                                                  gtggtgGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGNGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGt                                                                                                                                                                                                                                >  1:578041/1‑300 (MQ=255)                                                                  gtggtgGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAACCCCGATTGCGCTGATGGTGCTGACTGCTACGGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGt                                                                                                                                                                                                                                <  1:84833/300‑1 (MQ=255)                                                                  gtggtgGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCACACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGt                                                                                                                                                                                                                                <  1:31012/300‑1 (MQ=255)                                                                           gAACTTTCCCCTCTGCATCGGATAGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGCATGGCCAATGGGCCGCGAAAGCTGGCGTGATAAAGATTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTAGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGc                                                                                                                                                                                                                       <  2:227882/300‑1 (MQ=255)                                                                           gAACTTCCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAACCGATAATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCTTGAGAAAGATTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGGGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGTTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGc                                                                                                                                                                                                                       <  2:136162/300‑1 (MQ=255)                                                                                       cTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAgcggcgcgg                                                                                                                                                                                                           >  1:606468/1‑300 (MQ=255)                                                                                         gCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGcggcgcggcg                                                                                                                                                                                                         >  1:795266/1‑300 (MQ=255)                                                                                             cTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATc                                                                                                                                                                                                     >  1:658320/1‑300 (MQ=255)                                                                                               gctgactgatgTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAGAGAGTCGCACACCACGCGACTGGTCACCATTCAAGTGCTGTCGATAATCCAGATTGTGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGTGGCGTATCGc                                                                                                                                                                                                   >  2:152316/7‑300 (MQ=255)                                                                                               agtggaggATGTGTGGGGGCAAGCGATGATTTTCTCCAACCTGTTGCGGATTGCCTTCCGGGTATGGCCGGTGTGCTGAGAAAGCTGGCGGGATAAAGATTCGCACACCGTGCGACGGGTAACCATGACTGTGCTGTCGATAATCCCGATTGCGCTGAGGGTGCTGATTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGGACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGc                                                                                                                                                                                                   <  2:410735/298‑1 (MQ=255)                                                                                                 tggatgATGTGCTGGTGCAAGCGATGATTTTCTTCAACCTTCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGATTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCt                                                                                                                                                                                                 <  2:515464/300‑1 (MQ=255)                                                                                                      gatgTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGTCCGATGTGCCGCGAAAGCTGGCGTGATAAAGATTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGcgt                                                                                                                                                                                            <  2:791752/300‑1 (MQ=255)                                                                                                         gtgCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGcgtcgt                                                                                                                                                                                         >  1:775231/1‑300 (MQ=255)                                                                                                          tgCTGGGGCAAGCGAGGATTTTCTTCAACCCGCTGATGATTGCCTTCTTGGTATGGCCGATGTGCCGCGAAAGTTGGTGTGAGAAAGATTCGCACACCGTTCGACNGGGCACCATGACCGTGCTGTCGATAATCCAGATTGCGCGGAGGGTGTGGATTGCTACAGGCGACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATGGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTg                                                                                                                                                                                        <  2:711200/300‑1 (MQ=255)                                                                                                                       gatgatTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCg                                                                                                                                                                           <  2:478235/300‑1 (MQ=255)                                                                                                                                        tgctgctaATTGCCTTCCGGGTGTGGCCGATGTGCCGCGAAAGCTGGCGTGATAAGGAGTCGCACACCGTGCGACTGGTCACCATGACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAag                                                                                                                                                          <  1:352570/300‑1 (MQ=255)                                                                                                                                          ctgctgGTTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAagag                                                                                                                                                        <  1:422678/300‑1 (MQ=255)                                                                                                                                                      ttCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGt                                                                                                                                            >  1:711345/1‑300 (MQ=255)                                                                                                                                                             tATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACGTGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAc                                                                                                                                     <  1:360536/300‑1 (MQ=255)                                                                                                                                                             tATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAAc                                                                                                                                     <  1:815336/300‑1 (MQ=255)                                                                                                                                                                                aggtgtgtttGATAAAGATTCGCACACCATTCGACTGGTCACCATGACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGATTGCGACAGGCTATTTTTGCACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGTTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCAt                                                                                                                  <  2:9106/291‑1 (MQ=255)                                                                                                                                                                                       cGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACtt                                                                                                           >  1:650565/1‑299 (MQ=255)                                                                                                                                                                                                  gTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCAACCATTGCACTGGAGCAAGTTaa                                                                                                >  2:375239/1‑300 (MQ=255)                                                                                                                                                                                                  gTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACNGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTaa                                                                                                >  2:203902/1‑300 (MQ=255)                                                                                                                                                                                                  gTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGAAGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGCCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTaa                                                                                                >  2:486752/1‑300 (MQ=255)                                                                                                                                                                                                  gTCGCACACCATGCGACTGGTCACCATTAACGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTaa                                                                                                >  2:135217/1‑300 (MQ=255)                                                                                                                                                                                                    cGCACACCATGCGACGGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAcc                                                                                              <  1:813251/300‑1 (MQ=255)                                                                                                                                                                                                         acCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGANACCGTTTATATGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGNAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACcagcag                                                                                         <  2:642943/300‑1 (MQ=255)                                                                                                                                                                                                          ccATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGa                                                                                        >  1:184222/1‑300 (MQ=255)                                                                                                                                                                                                          ccATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGa                                                                                        >  2:785917/1‑300 (MQ=255)                                                                                                                                                                                                          ccATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAACAGCAGa                                                                                        >  2:688416/1‑300 (MQ=255)                                                                                                                                                                                                                       cACCGTTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTAc                                                                           <  2:765179/300‑1 (MQ=255)                                                                                                                                                                                                                         ccATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATNGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGANCAAGTTAACCAGCAGACGCTGCGTATTACCa                                                                         <  2:573089/300‑1 (MQ=255)                                                                                                                                                                                                                         ccATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCa                                                                         >  1:338094/1‑300 (MQ=255)                                                                                                                                                                                                                         ccATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCa                                                                         <  2:375361/300‑1 (MQ=255)                                                                                                                                                                                                                         ccATTACCGTGCTGTCGAGAATCCCGATGGCGCTGATGGTGCTGACGGCTACGGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCa                                                                         <  1:401585/300‑1 (MQ=255)                                                                                                                                                                                                                         ccATGACCGTGCGGTCGATAATCCCGATTGCGCTGGTGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCa                                                                         <  1:112433/300‑1 (MQ=255)                                                                                                                                                                                                                                        cGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTAACATGTTGCTGATGTTTg                                                          >  1:265741/1‑300 (MQ=255)                                                                                                                                                                                                                                           tAATCCNGATTGCGCGGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCNGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTgcgc                                                       <  2:674241/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tGATGGTGCTGACTGCTACAGGCTACTTCGACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGt                                        <  1:684958/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tGATGGTGCTGACTACTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGt                                        <  1:356338/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTgg                                   >  1:77785/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTGCTGACTGCTACAGGCTACTTCTACACTAAGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTgg                                   >  2:159639/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      cTGCTACAGGCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCCCCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGTAAttt                            >  2:251554/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ggCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGAt                    >  1:811352/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ggCTACTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACAGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGAt                    >  2:781622/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aCTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGa                >  2:28425/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCCGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGAt               >  1:683026/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGAt               >  2:241008/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGAt               >  1:4975/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAAACAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATCTGGTCCGATTTGAt               >  2:274512/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cTTCTACACTACGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCATGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTCCTGGGCAATCTGGTCCGacctgac               >  2:54829/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                             acccTGCGCCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTTAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTc     <  2:43206/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aCGCTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTc     >  2:103711/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTCAGc  <  1:346939/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGNCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGNAGAACCCACCATTGCACTTGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTCAGc  <  2:635229/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCGTCTGACAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCGGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCTTAGCGGCGCGGCGTATCGCCTGGCGNCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGCGGGCGCAGAAGGTGCTGAACCGCCGGNAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTCAGc  <  2:652454/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                cTGCGTCTGACAGGACGCTGGATTGAAACCGTTGATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTCAGc  <  2:317384/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   CCAGCCACTGGCGTCGGCAAATTGTCCGCATCAGTCTCGCATTGCTGCCTATCCATTTCTGGTCTGTGGTGGCAGAACTTTCCCCGCTGCATCTGATGGATGATGTGCTGGGGCAAGCGATGATTTTCTTCAACCTGCTGCTGATTGCCTTCCTGGTATGGCCGATGTGCCGCGAAAGCTGGCGTGATAAAGAGTCGCACACCATGCGACTGGTCACCATTACCGTGCTGTCGATAATCCCGATTGCGCTGATGGTGCTGACTGCTACAGGCTACTTCTACACTACGCTGCGTCTGGCAGGACGCTGGATTGAAACCGTTTATCTGGTGATCATCTGGAACCTGCTGTACCAGACGGTACTGCGTGGCTTAAGCGTAGCGGCGCGGCGTATCGCCTGGCGTCGTGCGCTGGCGCGTCGGCAGAATCTGGTGAAAGAGGGCGCAGAAGGTGCTGAACCGCCGGAAGAACCCACCATTGCACTGGAGCAAGTTAACCAGCAGACGCTGCGTATTACCATGTTGCTGATGTTTGCGCTGTTCGGTGTCATGTTCTGGGCAATTTGGTCCGATTTGATCACCGTGTTCAGC  >  NC_000913/488403‑488989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑