Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,078,342	G→T	R181S (CGC→AGC)	putA ←	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,078,342	0	G	T	100.0%	214.2 / NA	61	R181S (CGC→AGC)	putA	fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/35);  total (26/35)

GGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCT  >  NC_000913/1078073‑1078635                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      ggggaatgCTTACGCGGTGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCAATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCACACAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTAGCGAATTAACGCGTCGCTGGTGGCTTTGTAGGGAATACGCAACAACGc                                                                                                                                                                                                                                                                         >  2:523363/8‑300 (MQ=255) ggCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGc                                                                                                                                                                                                                                                                         >  1:124502/1‑300 (MQ=255)            cctcgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTGTGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGac                                                                                                                                                                                                                                                              <  2:333582/297‑1 (MQ=255)              gcgATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGacac                                                                                                                                                                                                                                                            <  2:767889/300‑1 (MQ=255)                     tttCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAA‑CAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAgcgc                                                                                                                                                                                                                                                    <  1:278518/300‑1 (MQ=255)                      ttCGCCAGTGACTAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAgcgc                                                                                                                                                                                                                                                    <  2:212517/300‑1 (MQ=255)                             gTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTAAGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCAAGCCt                                                                                                                                                                                                                                             >  2:94512/1‑300 (MQ=255)                                     cTGCTAACCCATCAGACGTATCGCCATGTCCACACCTTTGGGGATCAGCGGTTCACCTCTTTGATCGATAATGCGGTTCAGCAAGCGGGAGAGGCTTGCTTCGTTATGTGTGGAAACCAGGTGGCCAATAAACAACAGCCCCCAGGTGGCTGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGATTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTGTGTCTGGAATACACAACAACGCTTCCGCCAGACGCATCATCGCCACGCCCTCCTGCGa                                                                                                                                                                                                                                     <  2:259320/300‑1 (MQ=255)                                       gCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTTGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCNGCATTAACAAACAGTGACGGGCTACGNCCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATg                                                                                                                                                                                                                                   <  2:552644/300‑1 (MQ=255)                                         tCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGAc                                                                                                                                                                                                                                 <  1:680813/300‑1 (MQ=255)                                            cccATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGc                                                                                                                                                                                                                              >  2:628188/1‑300 (MQ=255)                                            cccATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGc                                                                                                                                                                                                                              >  1:372899/1‑300 (MQ=255)                                              cATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGa                                                                                                                                                                                                                            >  1:170309/1‑300 (MQ=255)                                                          cGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCaa                                                                                                                                                                                                                <  1:448859/300‑1 (MQ=255)                                                           gCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGANTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCAGCCAGACACATCAGCGCAACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAAt                                                                                                                                                                                                               >  2:187800/1‑300 (MQ=255)                                                           gCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAAt                                                                                                                                                                                                               >  1:537870/1‑300 (MQ=255)                                                                atCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAAccc                                                                                                                                                                                                          >  1:495721/1‑300 (MQ=255)                                                                atCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAAccc                                                                                                                                                                                                          >  2:523960/1‑300 (MQ=255)                                                                atCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGCAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAAccc                                                                                                                                                                                                          >  1:682574/1‑300 (MQ=255)                                                                      acCTTTGCTGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAc                                                                                                                                                                                                    <  2:131181/300‑1 (MQ=255)                                                                      acCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGNGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAc                                                                                                                                                                                                    <  2:196992/300‑1 (MQ=255)                                                                      acCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGGAAACAGCAGCCCCCAGGCGGCGGCATTAACAAACAGTGACGGGCTACGACCANTGTGTGACTGCCAGTTACCGTTGCTGNTTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAc                                                                                                                                                                                                    <  2:531441/300‑1 (MQ=255)                                                                      acCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGTTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAc                                                                                                                                                                                                    <  2:223237/300‑1 (MQ=255)                                                                      acCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAc                                                                                                                                                                                                    <  1:793911/300‑1 (MQ=255)                                                                       ccTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGANAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATNAGCGCCAAGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAcc                                                                                                                                                                                                   >  2:740428/1‑300 (MQ=255)                                                                       ccTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGAcc                                                                                                                                                                                                   >  2:515299/1‑300 (MQ=255)                                                                            gCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGGGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTTACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATAcc                                                                                                                                                                                              <  2:137994/300‑1 (MQ=255)                                                                            gCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATAcc                                                                                                                                                                                              <  1:725383/300‑1 (MQ=255)                                                                            gCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGTAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATAcc                                                                                                                                                                                              <  2:223089/300‑1 (MQ=255)                                                                            gCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCTGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATAcc                                                                                                                                                                                              <  2:240669/300‑1 (MQ=255)                                                                            gCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATAcc                                                                                                                                                                                              <  2:366236/300‑1 (MQ=255)                                                                            gCGGATCAGCGGTCCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATAcc                                                                                                                                                                                              <  1:89389/300‑1 (MQ=255)                                                                               gATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTgc                                                                                                                                                                                           >  1:614023/1‑300 (MQ=255)                                                                                    gCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGAc                                                                                                                                                                                      >  1:304077/1‑300 (MQ=255)                                                                                       gTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTNCGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGATGGNGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATCACAGCGAAAACTCCTGCAATAACCCATGGACCATACCTGCGCGACCAc                                                                                                                                                                                   >  2:645296/1‑300 (MQ=255)                                                                                                                     gcgagcgGGAGATGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCgg                                                                                                                                                     <  1:65757/300‑1 (MQ=255)                                                                                                                     gcgagcgGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCgg                                                                                                                                                     <  1:65748/300‑1 (MQ=255)                                                                                                                            ggAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTg                                                                                                                                              >  1:507013/1‑300 (MQ=255)                                                                                                                                 ggctggctTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGAAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATTGGCCAGCTGATACg                                                                                                                                         >  2:442859/1‑300 (MQ=255)                                                                                                                                   ctggctTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGcc                                                                                                                                       <  2:27364/300‑1 (MQ=255)                                                                                                                                     ggctTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCNCGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAg                                                                                                                                     >  1:593093/1‑300 (MQ=255)                                                                                                                                          cGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTtgt                                                                                                                                >  2:506966/1‑300 (MQ=255)                                                                                                                                                         ccAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCaa                                                                                                                 <  1:764668/300‑1 (MQ=255)                                                                                                                                                                    gTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGTTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGCTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGc                                                                                                      >  2:773675/1‑300 (MQ=255)                                                                                                                                                                                      gTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTGACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTNCTGCGATGACAGCGAAAACTCCTGCANTAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCcagc                                                                                    <  2:557158/300‑1 (MQ=255)                                                                                                                                                                                                 aacaaacaGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGTACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAAc                                                                         <  1:97710/300‑1 (MQ=255)                                                                                                                                                                                                 aacaaacaGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAAc                                                                         <  2:508507/300‑1 (MQ=255)                                                                                                                                                                                                               ggCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCNTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATNTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCCGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCg                                                           >  2:649766/1‑300 (MQ=255)                                                                                                                                                                                                               ggCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCg                                                           >  2:384300/1‑300 (MQ=255)                                                                                                                                                                                                                        cAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGAt                                                  <  1:253782/300‑1 (MQ=255)                                                                                                                                                                                                                          aTGTTTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAg                                                >  1:318805/1‑300 (MQ=255)                                                                                                                                                                                                                          aTGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGTAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTAGGTTTCAGGGCGGCGATAg                                                >  2:22257/1‑300 (MQ=255)                                                                                                                                                                                                                          aTGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGNAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGNCGATAg                                                >  2:675686/1‑300 (MQ=255)                                                                                                                                                                                                                            gtgtgACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGc                                              <  1:252604/300‑1 (MQ=255)                                                                                                                                                                                                                                   tGCCAGTTACCGTTGCTAATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTg                                       <  2:117728/300‑1 (MQ=255)                                                                                                                                                                                                                                           aCCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGTTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCNAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATcgcggc                               <  1:592413/300‑1 (MQ=255)                                                                                                                                                                                                                                           aCCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATcgcggc                               <  1:172166/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gATTTTTTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAAc                      <  2:216608/300‑1 (MQ=255)                                                                                                                                                                                                                                                         tGTCGCGAATTAACGCGTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACt                 <  1:785766/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       gcgTCGCTGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGc   <  2:506553/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        cgTCGCTGGTGGCTTTTTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGTACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTNTCGGCCAGCTGATACGCCAGTTTGTGNGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCt  <  2:556212/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      GGCTAACGCTTCCGCGATGGTTTCGCCAGTGACGAACTGCTCACCCATCAGGCGCATCGCCATATCCACACCTTTGCGGATCAGCGGTTCACCGCTTTTACCGATAATGCGGTTCAGCGAGCGGGAGAGGCTGGCTTCGTTATGGGTGGAAACCAGTTTGCCAGTAAACAGCAGCCCCCAGGTGGCGGCATTAACAAACAGTGACGGGCTACGACCAATGTGTGACTGCCAGTTACCGTTGCTGATTTTGTCGCGAATTAACGCGTCGCGGGTGGCTTTGTCGGGAATACGCAACAACGCTTCCGCCAGACACATCAGCGCCACGCCTTCCTGCGATGACAGCGAAAACTCCTGCAATAACCCCTGGACCATACCTGCGCGACCACTGGCATTTTTTTGATTACGCAGTTTATCGGCCAGCTGATACGCCAGTTTGTGCGCCTGTTCAGCAACTGGCTGCGGCAGGCGGGCTTGTTCCAGCAGCATAGAAACCGCTTCGGTTTCCGGGCGGCGATAGGCCGCGGTGATCGCGGCGCGGGAAACCGACTGGGGCAATATTTGCT  >  NC_000913/1078073‑1078635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑