Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,294,130	G→A	L259L (CTG→TTG)	ccmF ←	holocytochrome c synthase CcmF component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,294,130	0	G	A	100.0%	201.6 / NA	57	L259L (CTG→TTG)	ccmF	holocytochrome c synthase CcmF component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/30);  total (27/30)

AACATTGTTCGCTAACAGCAAAGATTCCCGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAG  >  NC_000913/2293834‑2294424                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        aaCATTGTTCGCTAACAGCAAAGATTCCCGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAacgc                                                                                                                                                                                                                                                                                                      <  1:68933/300‑1 (MQ=255)           gCTAACAGCAAAGATTCCCGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCccacc                                                                                                                                                                                                                                                                                            <  1:818814/300‑1 (MQ=255)           gCTAACAGCAAAGATTCACGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCccacc                                                                                                                                                                                                                                                                                            <  1:125411/300‑1 (MQ=255)               aCAGCAAAGACTCCCGCGGCCACGGTGCAGTGTTTACGCGTGAGCGAACTTTGTTTCTACGCGCGGCAAGCAGCAGTAGCGAACCGCCAGTCACCAGCACCATAAAGGCGAGGATGAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGGTACTAGCACGCCGGAACGCACGAGGAAAGTCCCCACCAGACACAACGAGGAGGCACTGATTGCCAGCAGGAATGTCCACGCTTTGAAGCTGTCGCGTTGTTCAGCGACCGCCAGTGAGTGCATCAACGCAGTCCccaccaccc                                                                                                                                                                                                                                                                                        <  2:221551/300‑4 (MQ=255)                  gCAAAGATTCCCGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACg                                                                                                                                                                                                                                                                                     >  1:768832/1‑300 (MQ=255)                           cccGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACg                                                                                                                                                                                                                                                                            <  1:57225/300‑1 (MQ=255)                             cgcgACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACTGCGCGCCGGATCAAACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCCCGAGGAAAGTCCCCAACAGACACACCGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGTGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTACCAAACAGCAAAGGCATAAACGAg                                                                                                                                                                                                                                                                          >  2:404468/1‑300 (MQ=255)                             cgcgACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCCCGAGGAAAGTCCACAACAGACACAACGAGAAGGCACTGATTGCAAGCAGTAATGTCCACGCTTTGCAGCTGGCGCGTTGTTCAGTGACCGCCAGCGAGTGCATCAACGCAGTCCCCACCAGCAACGGCATAAACGAg                                                                                                                                                                                                                                                                          >  2:403943/1‑300 (MQ=255)                                  ccACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGAGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCAAACAGGCACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGTTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCAAGGCATAAATGAGGCGtt                                                                                                                                                                                                                                                                     >  1:45172/1‑300 (MQ=255)                                            ttGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGa                                                                                                                                                                                                                                                           >  1:726727/1‑300 (MQ=255)                                              gTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATACAGGCGAGGATAACCATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGCACGCCCGAGGACCGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCCCGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCCGCAACGGCACAAACGAGGCGTTTTCCACCGGATc                                                                                                                                                                                                                                                         >  2:398849/1‑300 (MQ=255)                                              gTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATc                                                                                                                                                                                                                                                         >  1:405792/1‑300 (MQ=255)                                              gTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCAAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATc                                                                                                                                                                                                                                                         >  1:308250/1‑300 (MQ=255)                                              gTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCAAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATc                                                                                                                                                                                                                                                         >  1:191709/1‑300 (MQ=255)                                                   cgcgTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACNAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCANCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGa                                                                                                                                                                                                                                                    >  2:638213/1‑300 (MQ=255)                                                   cgcgTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCACGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGa                                                                                                                                                                                                                                                    <  1:356509/300‑1 (MQ=255)                                                          gCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAATGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAAccacca                                                                                                                                                                                                                                             <  2:630440/300‑1 (MQ=255)                                                                                     cagcagcagCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCACAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGc                                                                                                                                                                                                                  >  1:103774/1‑300 (MQ=255)                                                                                      agcagcngcGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGNGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGcc                                                                                                                                                                                                                 <  2:663534/300‑1 (MQ=255)                                                                                                       aaTCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCAc                                                                                                                                                                                                <  1:417305/300‑1 (MQ=255)                                                                                                          cACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGAt                                                                                                                                                                                             >  1:522862/1‑300 (MQ=255)                                                                                                            ccAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGc                                                                                                                                                                                           <  2:387774/300‑1 (MQ=255)                                                                                                                 aCCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGc                                                                                                                                                                                      <  1:276832/300‑1 (MQ=255)                                                                                                                            gAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAACGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACTAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATATGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGAt                                                                                                                                                                           <  1:92064/300‑1 (MQ=255)                                                                                                                               gATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCAGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCAAGCAGTAATGTCCACGCTTTGAAGCTGACGCGTTGTTCAGTGACCGCCAGTGCGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCAGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGCAGCTCCa                                                                                                                                                                        >  2:496464/1‑300 (MQ=255)                                                                                                                                   aaCATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGTAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTACCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCGCGCt                                                                                                                                                                    >  2:444237/1‑300 (MQ=255)                                                                                                                                                ccacaTCAGACACAAACGCGTGTACCGATACCAGCACGCCGGACCGCACGAGGAAAGTCCCAAACAGACACAACGAGCAGGCACTGCTTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCACCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTTGTCATCGGCCCCTGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCAACGCTGCCAGCGCCCACg                                                                                                                                                       >  2:122414/5‑300 (MQ=255)                                                                                                                                                ccGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACg                                                                                                                                                       >  1:773146/1‑300 (MQ=255)                                                                                                                                                                cgcgTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGNACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACgcgc                                                                                                                                       >  1:551854/1‑300 (MQ=255)                                                                                                                                                                        ccGATACCAGCACGCCGGAACGCACTAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGc                                                                                                                               <  2:449270/300‑1 (MQ=255)                                                                                                                                                                        ccGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCATTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGc                                                                                                                               <  2:26202/300‑1 (MQ=255)                                                                                                                                                                                  cACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACNAGGCGTTTTCTAACGGATCCCAGAACNACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACg                                                                                                                     <  2:533777/300‑1 (MQ=255)                                                                                                                                                                                  cACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACg                                                                                                                     <  2:473730/300‑1 (MQ=255)                                                                                                                                                                                    cGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGcc                                                                                                                   <  1:468279/300‑1 (MQ=255)                                                                                                                                                                                    cGCCGGAACGCACGAGGAAAGTACCCAACAGACACAACGAGAAGGCCCTGATTGCCAGCCGTAATGTCCACGCTTTGAAGCTGGAGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTCCCGGATCCCAGAACCAACAGCCACCCCAGCCGAGTTCGTCGTAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCAAGCGTCCACGGACGAGTAAAACGCACATAGGTGCTGTCCAGACGcc                                                                                                                   >  2:413054/1‑300 (MQ=255)                                                                                                                                                                                     gCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGccc                                                                                                                  <  1:375497/300‑1 (MQ=255)                                                                                                                                                                                              cACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCaa                                                                                                         >  1:463053/1‑300 (MQ=255)                                                                                                                                                                                                   ggAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAaagaag                                                                                                    <  1:376225/300‑1 (MQ=255)                                                                                                                                                                                                                ccgACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGNGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTAACGGACCCCAGAACCCCCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAANACGCGCATAAGTGCTGTCCAGATGCCCGCTCAGCAAAGAAGCAATGGCAAAAGc                                                                                       >  2:758345/3‑300 (MQ=255)                                                                                                                                                                                                                                  aCTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACTAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCAAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAATAAGcggtgaaggagacaaagaccaccgagaaacc                                                                     <  2:12566/300‑31 (MQ=255)                                                                                                                                                                                                                                           cagcatTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATTGCAAAAGCAAACG‑CCACCGAGAAACCCACGTAccc                                                           <  1:303080/300‑1 (MQ=255)                                                                                                                                                                                                                                           cagcagTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCCCCAGCCACGGCATAAACGAGGCGTTTTCTAACGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATCGACCCATGCGGACCCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGCAGAACGCGCCTAAGTGCTGTCCAGGCGCACGCTCAGCAAAGAAGCAACGGCCAAAGCAAACG‑CAACCGAGaacacaaagtaccc                                                           >  2:396931/1‑288 (MQ=255)                                                                                                                                                                                                                                           cagcagTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTAccc                                                           <  1:749229/300‑1 (MQ=255)                                                                                                                                                                                                                                                gtactGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCNACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCATAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCCGACGCCCGCTCNGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCatat                                                      >  2:745705/5‑300 (MQ=255)                                                                                                                                                                                                                                                gTAATGGCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGATGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCTAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCATGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAATAAGCACTGGCAAAGACAACCA‑CCACCGAGAAACCCACGTACCCCatat                                                      <  2:27181/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aaTGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCNACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCNGCAAAGAAGCAATGGCAAAAGCGAACG‑CCACCGAGAAACCCACGTACCCCATATaa                                                    <  2:749772/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCCTCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCANCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGANGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAGAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGc                                                 <  2:648862/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACNCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATATGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAATAAGCAATGGAAAAAACGAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGc                                                 <  2:606924/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCAGAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAGTGG‑AAGAGGCGACGACCACCGAGAAACCCACGTACCCCATATAAAGc                                                 <  2:171014/300‑1 (MQ=255)                                                                                                                                                                                                                                                            cTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCg                                          >  1:628631/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gcgTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACTAGGCGTTTTCGACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCNAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACCCGCATAAGTGCTGTCCAGACGCCCACTCAGCAAAGAAGCAATGGCGAAAACAGAAA‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                             <  2:190498/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gcgTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                             >  1:42744/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gcgTTGTTCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTNCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAg                             <  1:575365/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            ttgttCAGTGACCGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCATACTCCCTCTCCTCACCTCCTCACTTACAGACACAACCA‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGccc                          <  2:290270/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ccGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCa               >  1:369584/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        cGCCAGTGAGTGCATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAATAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCaa              <  2:504063/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    cATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGCTCCTGCAATAGCGGGTTAAg  >  1:360899/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    cATCAACGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAg  >  1:750120/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        AACATTGTTCGCTAACAGCAAAGATTCCCGCGACCACAGCGCATTGTTTACGCGTGAGCGAACTTTGTGTCCACGCGCGGCAAACAGCAGCAGCGAACCGCCAATCACCAGCACCATAAAGGCGAGGATAAACATACCGCGCGCCGGATCAGACGCAAACGCGTGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCGAGCGTCAGGAAGATCCACGCTGCCAGCGTCCACGGACGAGTAAAACGCGCATAAGTGCTGTCCAGACGCCCGCTCAGCAAAGAAGCAATGGCAAAAGCAAACG‑CCACCGAGAAACCCACGTACCCCATATAAAGCAGAGGCGGATGGAAGATCAGCCCCGGATCCTGCAATAGCGGGTTAAG  >  NC_000913/2293834‑2294424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑