Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,894,704	C→T	S18L (TCA→TTA)	yieF →	quinone reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,894,704	0	C	T	98.9%	320.1 / NA	92	S18L (TCA→TTA)	yieF	quinone reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (47/44);  minor base G (0/1);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGC  >  NC_000913/3894414‑3894999                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        tCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATgg                                                                                                                                                                                                                                                                                                <  1:176776/300‑1 (MQ=255)  cctGTAAATCATGTAGAAGCGTTATGCAACGCGGAAGACGTGCTGGTGTGGTCTGTGGCGGTCACGCCCACGATTGAAGAGCTCAGTGTCGGGGCGTTAGATGGAAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTAACAGCCGGATGATGCGTTTTGCCCACCTCTCTACCGTGAAGGCTTTTTCGCCAAATTGCTAGACAACCCTAGGAGTCACCATGTCTGGAAAATTTCCGGTGGTTACGTTACTGGGGAGCATGCGCAAAGGCTTATTTAATGGc                                                                                                                                                                                                                                                                                               >  2:273547/3‑300 (MQ=255)  cATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACATGGCTGGAAAAGCCTGCCGGATATTCAAAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGCTAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGTCTGCGCCAAGGCTTATTTAATGGc                                                                                                                                                                                                                                                                                               >  2:82284/1‑300 (MQ=255)  cATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTANCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGc                                                                                                                                                                                                                                                                                               >  1:553430/1‑300 (MQ=255)  cATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGc                                                                                                                                                                                                                                                                                               >  1:684140/1‑300 (MQ=255)    tGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCAt                                                                                                                                                                                                                                                                                             <  2:383234/300‑1 (MQ=255)       aaaTCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAAGTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGt                                                                                                                                                                                                                                                                                          <  2:308578/300‑1 (MQ=255)            aTGTAGAAGCGTTATTCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACTGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCAc                                                                                                                                                                                                                                                                                     <  1:522089/300‑1 (MQ=255)            aTGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCAc                                                                                                                                                                                                                                                                                     <  1:522090/300‑1 (MQ=255)             tGTAGAAGCGCTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACg                                                                                                                                                                                                                                                                                    <  1:122404/300‑1 (MQ=255)              gTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGt                                                                                                                                                                                                                                                                                   <  2:260021/300‑1 (MQ=255)                aGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTAc                                                                                                                                                                                                                                                                                 <  1:743755/300‑1 (MQ=255)                  aaGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTAccc                                                                                                                                                                                                                                                                               <  1:190525/300‑1 (MQ=255)                    gCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTg                                                                                                                                                                                                                                                                             >  1:663535/1‑300 (MQ=255)                              aCGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGNGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGNAAATTg                                                                                                                                                                                                                                                                   >  2:666104/1‑300 (MQ=255)                              aCGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTg                                                                                                                                                                                                                                                                   >  1:751168/1‑300 (MQ=255)                                 cgGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCNTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAANTTGCTc                                                                                                                                                                                                                                                                >  2:661073/1‑300 (MQ=255)                                        cttGCTGGTTTGTTCCGTGGCGTTCACGCCCACAATTGAAAAGCTCAGTGTCTGTGAGTTAGATTGCAAACACGGCTGGAAAAGCTTGCCGGAGATTCACAGCCGCGCCAACAATCCTACCAGCTGGGTGATGCGTTTTGCCCAACTCTCTACCGTGAAGGTTTTTTCGCTAAATTGATGGACAACCACAGGAGTCATCATGTCTGAAAAATGGCAGTTGGTGATGCTGCTGGTGATCCTGCGCAAATGCTTATTTACTGTCGTGGGTGCACGTAcctgtctcttatacacatctcgcag                                                                                                                                                                                                                                                         <  1:339308/298‑24 (MQ=255)                                         gTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGc                                                                                                                                                                                                                                                        >  1:227355/1‑300 (MQ=255)                                              ggTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGGTGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCCTGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGa                                                                                                                                                                                                                                                   >  2:440305/1‑300 (MQ=255)                                              ggTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGa                                                                                                                                                                                                                                                   >  1:158740/1‑300 (MQ=255)                                                 gtgGTCCGTGGCGGTCACGCCCACGATTTAAAAGCTCAGTGTCTGGTAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACATGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAAGGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGt                                                                                                                                                                                                                                                <  2:300078/300‑1 (MQ=255)                                                        gTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTNTTCGCCAAATTGATAGACAACCACAGNAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCg                                                                                                                                                                                                                                         <  2:548146/300‑1 (MQ=255)                                                             ggTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTAcc                                                                                                                                                                                                                                    >  1:800679/1‑300 (MQ=255)                                                                   gCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTAccatccat                                                                                                                                                                                                                              >  1:733842/1‑300 (MQ=255)                                                                   gCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTAACGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACACTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTAcgatccat                                                                                                                                                                                                                              >  2:93583/1‑300 (MQ=255)                                                                          aTTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGAc                                                                                                                                                                                                                       <  1:426452/300‑1 (MQ=255)                                                                                      aGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTAt                                                                                                                                                                                                           <  1:526959/300‑1 (MQ=255)                                                                                       gtgtCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCTGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCAGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATg                                                                                                                                                                                                          >  1:462059/1‑300 (MQ=255)                                                                                       gtgtCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATg                                                                                                                                                                                                          >  1:538129/1‑300 (MQ=255)                                                                                       gtgtCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGAAATTCCCTTGTATg                                                                                                                                                                                                          >  1:71178/1‑300 (MQ=255)                                                                                         gtCTGGGAGTTAGATGGCAAACACGGCTGGANAAGCCTGCCGGATCTCCAAAGCCGCGCCAACAATCCCCCCAGCCGGACGATACACTTTGCCCAACTCTCTACCGTGCAGTCTCTTTCGCCCANTTGCTAGACCCCCACCGGCGTCATCATGTCTGCAACATCGCAGGTGGTTCCGTTACTGGGGAGCCTGCGCAAAGGCTTCTTTAATGGCACGGTTGCACGTACCCTGCCGAAAATTGCCCCGGCGAGCATGGAAGTCAATGTGTTACCATCCATTGCCGACATTCCATTGTATGAc                                                                                                                                                                                                        >  2:585322/1‑300 (MQ=255)                                                                                         gtCTGGGAGTTAGATGGCAAACACGGCTGGANAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAANTTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGACGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGaa                                                                                                                                                                                                        >  2:585208/1‑299 (MQ=255)                                                                                         gtCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGAc                                                                                                                                                                                                        >  2:791851/1‑300 (MQ=255)                                                                                           ctaggAGTTAGATGGCAAACACTGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACTAGTCGGATGATGCGTTTTGCCCAACCCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCAGCATGTCTGAAAAATTGCAGGTGGTTACGTGACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGTTTGCACGTACCCTGCCGAAAATTACTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGc                                                                                                                                                                                                      <  2:2818/297‑1 (MQ=255)                                                                                           cTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCAGACATTCCCTTGTATGACGc                                                                                                                                                                                                      <  1:694796/300‑1 (MQ=255)                                                                                               gAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAANAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGNATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGAc                                                                                                                                                                                                  >  2:757531/1‑300 (MQ=255)                                                                                                   tAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAc                                                                                                                                                                                              <  2:438108/300‑1 (MQ=255)                                                                                                     gacggtaaaCATGGCTGGAGAAGCCTGCCGAAGATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACCCTCTACCGTGAATGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGTTTACGTTACTGGGGAGCCTACGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAcag                                                                                                                                                                                            <  2:357851/294‑1 (MQ=255)                                                                                                     gATGGCAAACACGGCTGTAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAcag                                                                                                                                                                                            <  2:442802/300‑1 (MQ=255)                                                                                                      aTGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACATGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAcagc                                                                                                                                                                                           <  1:330186/300‑1 (MQ=255)                                                                                                        ggCAAACACGGCTGGAAAAGCCGGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCNTCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTAcagcag                                                                                                                                                                                         <  2:190161/300‑1 (MQ=255)                                                                                                           aaaCACGGCTGGAAAAGCCTGCCTGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACCCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCCCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGgaa                                                                                                                                                                                      <  2:16811/300‑1 (MQ=255)                                                                                                                           gCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCaa                                                                                                                                                                      <  1:279912/300‑1 (MQ=255)                                                                                                                                      atTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGct                                                                                                                                                           >  1:214509/1‑300 (MQ=255)                                                                                                                                      atTCACAGCCGCGCCAACAATCCTACCAGACGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGct                                                                                                                                                           >  2:735275/1‑300 (MQ=255)                                                                                                                                          acaGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTgg                                                                                                                                                       <  1:536594/300‑1 (MQ=255)                                                                                                                                                   ccAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTNACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGNAACAGa                                                                                                                                              >  2:667432/1‑300 (MQ=255)                                                                                                                                                         aTCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTAACGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTc                                                                                                                                        >  2:251135/1‑300 (MQ=255)                                                                                                                                                                       atgatgCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGtgtg                                                                                                                          >  1:692977/1‑300 (MQ=255)                                                                                                                                                                           tgCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGa                                                                                                                      <  2:677354/300‑1 (MQ=255)                                                                                                                                                                             cggtttGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACATGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATc                                                                                                                    <  1:782775/297‑1 (MQ=255)                                                                                                                                                                             cGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATc                                                                                                                    <  1:296565/300‑1 (MQ=255)                                                                                                                                                                                tttGCCCAACCCTTTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTc                                                                                                                 <  2:221689/300‑1 (MQ=255)                                                                                                                                                                                        aCTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGa                                                                                                         >  2:73844/1‑300 (MQ=255)                                                                                                                                                                                               accttGAATGCTTTTGCGCCAAATCGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAac                                                                                                  <  2:79832/296‑1 (MQ=255)                                                                                                                                                                                               aCCGTGNAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTNAAAAAGTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAac                                                                                                  <  2:658496/300‑1 (MQ=255)                                                                                                                                                                                               aCCGTGGAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCANTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGNCGGTGTGGTGATCGTCACGCCGGAATATAac                                                                                                  <  2:561832/300‑1 (MQ=255)                                                                                                                                                                                               aCCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAac                                                                                                  <  1:122688/300‑1 (MQ=255)                                                                                                                                                                                                    gAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTc                                                                                             <  1:280244/300‑1 (MQ=255)                                                                                                                                                                                                         cTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTAc                                                                                        <  1:358596/300‑1 (MQ=255)                                                                                                                                                                                                              tcgacAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGt                                                                                   <  1:776689/296‑1 (MQ=255)                                                                                                                                                                                                              tCGCCAAATTGATAGACAACCACAGTAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATTGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGt                                                                                   <  1:408354/300‑1 (MQ=255)                                                                                                                                                                                                              tCGCCAAATTGATAGACAACCACAGGATGCATCATGTCTGAAAAATTGCAGGTGTTTACGTTACTGGGGATCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTTTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGt                                                                                   <  2:337032/300‑1 (MQ=255)                                                                                                                                                                                                                    aaTTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTg                                                                             >  1:638646/1‑300 (MQ=255)                                                                                                                                                                                                                        gATATACAACCACAGGAGTCATCATGTCTGAAAAATTGCATGTGGTTACGTTACTTGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACATACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGaaaa                                                                         <  2:393428/300‑1 (MQ=255)                                                                                                                                                                                                                                 ccACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCg                                                                >  1:498486/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATCCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCANCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCg                                                                >  2:703063/1‑300 (MQ=255)                                                                                                                                                                                                                                         gTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTAATCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCtt                                                        >  1:813547/1‑300 (MQ=255)                                                                                                                                                                                                                                                        aaaTTGCAGGTGGTTCCGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGAt                                         >  1:709141/1‑300 (MQ=255)                                                                                                                                                                                                                                                        aaaTTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGAt                                         >  1:342197/1‑300 (MQ=255)                                                                                                                                                                                                                                                              cgggtggtTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCg                                   <  2:382444/298‑1 (MQ=255)                                                                                                                                                                                                                                                               aGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGGACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGc                                  <  2:734035/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gttacgttacTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCAGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGcc                             <  2:232482/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     ttacgttacTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCg                            >  2:483765/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ttacTGGGGAGCCTGCGCAAAGGCTTATTTANTGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCATTGTATGACGNTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTaaa                       >  2:587836/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ttacTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTaaa                       >  1:380901/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ttacTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTaaa                       >  2:726302/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            acTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAAcc                     >  1:607120/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            acTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCATTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCAGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATTACCCGCTGGCCGGTAAAcc                     >  2:791349/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             cTGGGGAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCg                    >  1:511508/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               ggggAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACGGATCCGTCAGGCTGCCGGTGTGGTGATCGTCCCGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCATACCGGATCAACCGCTGGCCGGTAAACCGGt                  >  2:337679/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 tgAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTAt                <  1:59043/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ggAGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTAt                <  1:8384/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aGCCTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGCATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTCACACGGTATTg              >  2:78980/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cTGCGCAAAGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATTCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGAAGGTGTGGTGATCGTCAAGCAGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGATGGCCGGTAAACAGGTATTGAtt           >  2:440601/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       tGCGCAAAGGCTGATTTGATGGCAGGTTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGGACCGGGGGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCAGGATCAACCGCTGGCCGGTAAACCGGTATTGATTc          <  1:261831/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aaaGGCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGAcc     <  1:814132/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               ggCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCANGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGc  >  1:550128/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               ggCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGc  >  2:728966/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               ggCTTATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGc  >  1:781329/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               ggCTTATTTAATGGCATGGTTGCACGTACCCNGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGNTTTTCCAGCAACGGTTGAAGCTCTGGGGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCATGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCACACCAGc  >  2:568390/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        TCATGTAAATCATGTAGAAGCGTTATGCGACGCGGAAGACGTGCTGGTGTGGTCCGTGGCGGTCACGCCCACGATTGAAAAGCTCAGTGTCTGGGAGTTAGATGGCAAACACGGCTGGAAAAGCCTGCCGGATATTCACAGCCGCGCCAACAATCCTACCAGCCGGATGATGCGTTTTGCCCAACTCTCTACCGTGAAGGCTTTTTCGCCAAATTGATAGACAACCACAGGAGTCATCATGTCTGAAAAATTGCAGGTGGTTACGTTACTGGGGAGCCTGCGCAAAGGCTCATTTAATGGCATGGTTGCACGTACCCTGCCGAAAATTGCTCCGGCGAGCATGGAAGTCAATGCGTTACCATCCATTGCCGACATTCCCTTGTATGACGCTGACGTACAGCAGGAAGAAGGTTTTCCAGCAACGGTTGAAGCTCTGGCGGAACAGATCCGTCAGGCTGACGGTGTGGTGATCGTCACGCCGGAATATAACTACTCGGTACCGGGTGGGCTGAAAAATGCCATCGACTGGCTTTCCCGCCTGCCGGATCAACCGCTGGCCGGTAAACCGGTATTGATTCAGACCAGC  >  NC_000913/3894414‑3894999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑