Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,701,372	C→T	A139V (GCT→GTT)	malY →	negative regulator of MalT activity/cystathionine beta‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,701,372	0	C	T	100.0%	210.7 / NA	58	A139V (GCT→GTT)	malY	negative regulator of MalT activity/cystathionine beta‑lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/29);  total (29/29)

ACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATA  >  NC_000913/1701080‑1701665                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      aCTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTagag                                                                                                                                                                                                                                                                                                <  1:689100/300‑1 (MQ=255) aCTGCCCCCTGCATTATCGAGGCACTGAATCAGCGCCTGATGCAAGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCACTACACCGCCATCGATTCTCAGACGGTGGTGGATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTCTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTagag                                                                                                                                                                                                                                                                                                <  1:673163/300‑1 (MQ=255)         cTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCACGTTGTTTTAGAGAAGCAGGc                                                                                                                                                                                                                                                                                        >  2:486969/1‑300 (MQ=255)           gCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTg                                                                                                                                                                                                                                                                                      >  1:683806/1‑300 (MQ=255)           gCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTg                                                                                                                                                                                                                                                                                      >  2:624777/1‑300 (MQ=255)               tATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGAtgg                                                                                                                                                                                                                                                                                  <  1:730351/300‑1 (MQ=255)                    aGGCGCTGAATCAGCGCCGGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGAtggttggt                                                                                                                                                                                                                                                                             <  2:436141/300‑1 (MQ=255)                           gAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGa                                                                                                                                                                                                                                                                      >  1:190891/1‑300 (MQ=255)                                    ccTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACGAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCaa                                                                                                                                                                                                                                                             >  2:489748/1‑300 (MQ=255)                                        aTGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGATGATGGTTGGTTTTGCGATATGGGCAAGTTg                                                                                                                                                                                                                                                         >  1:183137/1‑300 (MQ=255)                                            aCGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTTCGATATGGGCAAGTTGGAAg                                                                                                                                                                                                                                                     <  1:606794/300‑1 (MQ=255)                                            aCGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAg                                                                                                                                                                                                                                                     <  1:606627/300‑1 (MQ=255)                                             cGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGTTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGc                                                                                                                                                                                                                                                    <  2:412694/300‑1 (MQ=255)                                             cGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGc                                                                                                                                                                                                                                                    <  1:152531/300‑1 (MQ=255)                                             cGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGc                                                                                                                                                                                                                                                    <  2:357127/300‑1 (MQ=255)                                                       ggCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCg                                                                                                                                                                                                                                          <  1:661371/300‑1 (MQ=255)                                                                                  tttCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTATAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCtg                                                                                                                                                                                                               <  1:370856/300‑1 (MQ=255)                                                                                       cgcgGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAg                                                                                                                                                                                                          <  1:656047/300‑1 (MQ=255)                                                                                           gCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCca                                                                                                                                                                                                      >  2:89938/1‑300 (MQ=255)                                                                                              aTTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAg                                                                                                                                                                                                   <  1:502784/300‑1 (MQ=255)                                                                                                        ggTTTCCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATTGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCg                                                                                                                                                                                         <  2:80528/300‑1 (MQ=255)                                                                                                                     gCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGAc                                                                                                                                                                            >  2:376472/1‑300 (MQ=255)                                                                                                                     gCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGAc                                                                                                                                                                            >  1:635284/1‑300 (MQ=255)                                                                                                                     gCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTAGTCTATATGGTTTCAGAATTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCATGTGTAGCCCACAGAATCCTAACGGGAAGGTGTGGAc                                                                                                                                                                            >  2:36480/1‑300 (MQ=255)                                                                                                                      cATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCGATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACg                                                                                                                                                                           <  1:56836/300‑1 (MQ=255)                                                                                                                               gCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCAGAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTNTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAg                                                                                                                                                                  >  2:708085/1‑300 (MQ=255)                                                                                                                                      aTTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCATGTGTAGCCCACAGAATCCTAACGGGAAAGTGTGGACGTGCGATGAGCTGgaga                                                                                                                                                           >  2:75572/1‑300 (MQ=255)                                                                                                                                                 gtggtgTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCGCAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGAc                                                                                                                                                >  2:764976/1‑300 (MQ=255)                                                                                                                                                 gtggtgTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGAc                                                                                                                                                >  1:613715/1‑300 (MQ=255)                                                                                                                                                  tggtgTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGAcc                                                                                                                                               <  1:253784/300‑1 (MQ=255)                                                                                                                                                    gtgtATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGCTCATGGCTGACCtg                                                                                                                                             >  2:441063/1‑300 (MQ=255)                                                                                                                                                      gtATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGAGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCtgtg                                                                                                                                           >  2:144947/1‑300 (MQ=255)                                                                                                                                                                tCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCAGTGTTGGCGAAACCAGAATGTAAAATTATGCGCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCAt                                                                                                                                 >  2:695076/1‑300 (MQ=255)                                                                                                                                                                  tGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTATAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATgg                                                                                                                               <  1:318585/300‑1 (MQ=255)                                                                                                                                                                   gTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGgt                                                                                                                              >  2:447780/1‑300 (MQ=255)                                                                                                                                                                    tcaccTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGgtg                                                                                                                             <  2:216142/296‑1 (MQ=255)                                                                                                                                                                    tCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAATGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTGAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTNGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGANATCATGGCTGACCTGTGCGAGCGTCATGgtg                                                                                                                             <  2:571209/300‑1 (MQ=255)                                                                                                                                                                            atGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGtt                                                                                                                     <  1:11173/300‑1 (MQ=255)                                                                                                                                                                                           aTTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCTGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATc                                                                                                      <  2:294849/300‑1 (MQ=255)                                                                                                                                                                                                      gTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCatatggatat                                                                                           >  1:91392/1‑300 (MQ=255)                                                                                                                                                                                                                                     acacCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTgg                                                            <  1:300320/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCt                                                <  1:87443/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTcgc                                             >  1:647386/1‑300 (MQ=255)                                                                                                                                                                                                                                                          tACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTATATAAGCAGGCTGATGGTTGGTTTTTCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTTTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTTCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCAGATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGAc                                       <  1:725498/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGAc                                       <  1:332687/300‑1 (MQ=255)                                                                                                                                                                                                                                                              aGGCCATTGAAGGTAACCAGCGCACAGTAATNCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTNCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGCCGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGCGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTggg                                   >  2:585723/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gCCATTGAAGGTAACCAGCGCACAGTAATGCTCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCg                                 <  2:60225/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       aaGGTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTaa                          >  2:769252/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ggTAACCAGCGCACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCNTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACg                        >  1:565129/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              ccAGCGCACAGTAATGCCCGTTGTTTGAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCggg                   <  1:276734/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gcgcACAGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTc                >  2:456546/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      caGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGCTGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGaaaa           >  2:91267/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       aGTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAg          <  1:147770/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGGGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATNTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGt         >  2:706070/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGt         >  2:721679/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGt         >  1:276580/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gTAATGCCCGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGAAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGt         >  2:514995/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               ccGTTGTTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAata  <  1:499881/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                      ACTGCCCCCTGCATTATCGAGGCGCTGAATCAGCGCCTGATGCACGGCGTATTTGGCTACAGCCGCTGGAAAAACGATGAGTTTCTCGCGGCTATTGCCCACTGGTTTTCCACCCAGCATTACACCGCCATCGATTCTCAGACGGTGGTGTATGGCCCTTCTGTCATCTATATGGTTTCAGAACTGATTCGTCAGTGGTCTGAAACAGGTGAAGGCGTGGTGATCCACACACCCGCCTATGACGCATTTTACAAGGCCATTGAAGGTAACCAGCGCACAGTAATGCCCGTTGCTTTAGAGAAGCAGGCTGATGGTTGGTTTTGCGATATGGGCAAGTTGGAAGCCGTGTTGGCGAAACCAGAATGTAAAATTATGCTCCTGTGTAGCCCACAGAATCCTACCGGGAAAGTGTGGACGTGCGATGAGCTGGAGATCATGGCTGACCTGTGCGAGCGTCATGGTGTGCGGGTTATTTCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATA  >  NC_000913/1701080‑1701665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑