Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,522,059	C→T	E231E (GAG→GAA)	hofM ←	DNA utilization protein HofM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,522,059	0	C	T	100.0%	196.1 / NA	55	E231E (GAG→GAA)	hofM	DNA utilization protein HofM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/31);  total (24/31)

GTTATCTGTAAGCTGCGGGCGAGTTGTTGTTCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCG  >  NC_000913/3521770‑3522350                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                    gTTATCTGTAAGCTGCGGGCGAGTTGTTGTTCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTACTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCACCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCATGGAt                                                                                                                                                                                                                                                                                           <  2:298409/300‑1 (MQ=255)     tCTGTAAGCTGCGGGCGAGTTGTTGTTCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCaaa                                                                                                                                                                                                                                                                                       >  1:344419/1‑300 (MQ=255)     tCTGTAAGCTGCGGGCGAGTTGTTGTTCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCaaa                                                                                                                                                                                                                                                                                       <  2:170562/300‑1 (MQ=255)      cTGTAAGCTGCGGGCGAGTTGTTGTTCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAAt                                                                                                                                                                                                                                                                                      >  1:39811/1‑300 (MQ=255)                       gttgttgttCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACatat                                                                                                                                                                                                                                                                     >  2:350636/1‑300 (MQ=255)                        ttgttgttCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAANTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTACAAGGGATCAAATCCGCCTTCGCCACATATc                                                                                                                                                                                                                                                                    >  2:181857/1‑300 (MQ=255)                        ttgttgttCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAANTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGACTTCCCAGGGATCAAATCCGCCTTCGCAACATATc                                                                                                                                                                                                                                                                    >  2:183439/1‑300 (MQ=255)                                      gAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTANCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCggg                                                                                                                                                                                                                                                      <  2:592494/300‑1 (MQ=255)                                              aCTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATAAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACgg                                                                                                                                                                                                                                              <  1:759553/300‑1 (MQ=255)                                               cTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGa                                                                                                                                                                                                                                             >  1:493428/1‑300 (MQ=255)                                                   gTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAAc                                                                                                                                                                                                                                         <  1:511346/300‑1 (MQ=255)                                                              cTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAg                                                                                                                                                                                                                              >  1:686031/1‑300 (MQ=255)                                                                 cGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTc                                                                                                                                                                                                                           <  1:738958/300‑1 (MQ=255)                                                                          cTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTCCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCt                                                                                                                                                                                                                  >  2:688621/1‑300 (MQ=255)                                                                              cAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAggc                                                                                                                                                                                                              <  2:39697/300‑3 (MQ=255)                                                                              cAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTc                                                                                                                                                                                                              <  1:660365/300‑1 (MQ=255)                                                                                   gCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGTCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATccc                                                                                                                                                                                                         <  1:330403/300‑1 (MQ=255)                                                                                      gTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAANAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGAGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCAc                                                                                                                                                                                                      >  2:713647/1‑300 (MQ=255)                                                                                      gTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCAc                                                                                                                                                                                                      >  1:153229/1‑300 (MQ=255)                                                                                      gTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGATTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATAGCGACGCTTTAGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCacac                                                                                                                                                                                                      >  2:479499/1‑300 (MQ=255)                                                                                          tAGCGTTATCCCGACTGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGcc                                                                                                                                                                                                  <  2:86103/300‑1 (MQ=255)                                                                                           aGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCa                                                                                                                                                                                                 >  2:439047/1‑300 (MQ=255)                                                                                           aGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGACAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATccacaatgcca                                                                                                                                                                                                 >  2:439976/1‑291 (MQ=255)                                                                                                            cagcagcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCta                                                                                                                                                                                >  2:494072/1‑300 (MQ=255)                                                                                                               cagcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAg                                                                                                                                                                             <  1:316560/300‑1 (MQ=255)                                                                                                               cagcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGNTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAg                                                                                                                                                                             <  2:595890/300‑1 (MQ=255)                                                                                                                 gcagAGNCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTNCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgcg                                                                                                                                                                           <  2:663770/300‑1 (MQ=255)                                                                                                                 gcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgtg                                                                                                                                                                           <  1:765856/300‑3 (MQ=255)                                                                                                                 gcagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgcg                                                                                                                                                                           <  1:82435/300‑1 (MQ=255)                                                                                                                 gcagAGGCGCAACGAACATAAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgcg                                                                                                                                                                           <  1:616309/300‑1 (MQ=255)                                                                                                                  cagAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAgcgc                                                                                                                                                                          <  2:774413/300‑1 (MQ=255)                                                                                                                         gcAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGccc                                                                                                                                                                   <  1:495130/300‑1 (MQ=255)                                                                                                                              gAACATCAGCAACCAGAAACGCAGAAAAGCGGGCCGGCGGTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGc                                                                                                                                                              <  1:350548/300‑1 (MQ=255)                                                                                                                                           cataaaCGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAGAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTGGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCTGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTAt                                                                                                                                                 <  2:413432/297‑1 (MQ=255)                                                                                                                                           cAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAGCAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCACCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTAt                                                                                                                                                 <  2:413605/300‑1 (MQ=255)                                                                                                                                           cAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGGTAt                                                                                                                                                 <  1:308848/300‑1 (MQ=255)                                                                                                                                                          gCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGAc                                                                                                                                  <  1:240798/300‑1 (MQ=255)                                                                                                                                                           cGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACa                                                                                                                                 <  1:120566/300‑1 (MQ=255)                                                                                                                                                             gTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACAtt                                                                                                                               >  1:731135/1‑300 (MQ=255)                                                                                                                                                                      tGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATgag                                                                                                                      <  1:196256/300‑1 (MQ=255)                                                                                                                                                                       gCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGACAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATCGCTGATgaga                                                                                                                     >  2:326586/1‑300 (MQ=255)                                                                                                                                                                            cGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTa                                                                                                                >  1:816505/1‑300 (MQ=255)                                                                                                                                                                                                    gggTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATg                                                                                        <  1:360895/300‑1 (MQ=255)                                                                                                                                                                                                                 cGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCgg                                                                           <  1:372128/300‑1 (MQ=255)                                                                                                                                                                                                                   ccTTCCCACGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTGCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCGACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCggtg                                                                         <  2:143652/300‑3 (MQ=255)                                                                                                                                                                                                                   ccTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCgggg                                                                         <  1:648690/300‑1 (MQ=255)                                                                                                                                                                                                                              gcCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTc                                                              >  1:40238/1‑300 (MQ=255)                                                                                                                                                                                                                                  gCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTTTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACAt                                                          >  1:651035/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTg                                        >  1:501748/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACNGCCAGTTTGCGCCCCCAGCTATAGCGNGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGGTCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTg                      >  2:535324/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gTAGCGGCGGCTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACGTTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTg                      >  1:75907/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             cggcTGACGAACAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCNCCCACAGCCACTGTTCGTTATCACGCNAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGctc               <  2:548933/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    cGAACATAAACGGCTTCCCATTGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTTTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTctttgctt        <  2:2029/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aaCAGAAACGGCTTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCACACAGCCACTGTTCGTTATCACGCCNGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCACCCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTctttgctttg      >  2:184807/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gAAACGTCTTCCCAGGGATCAAATCCGCCTTAGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCACACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGcgcg  >  1:426183/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                    GTTATCTGTAAGCTGCGGGCGAGTTGTTGTTCCGCCTGAAGCAAAACTGCGTCTATGCGCGCTTCGGCGCTGCCTGTCAGACGCAGTATTAGCGTTATCCCGACGGCCAGCAGCAGAGGCGCAACGAACATCAGCAACCAGAAACGCAGAAAAGCGGTCCGGCGTTGCTGTCGCCAGGGCAAAAAATTAATTGGCGGGTTCATCAGTACGCCTTCCCAAGCGCCAGCCCCAGCGCGATGGCAAAGTCTCCACCTGGCGGCGGTAGCGGCGGCTGACGAACAGAAACGGCCTCCCAGGGATCAAATCCGCCTTCGCCACATATCGCGACGCTTTCGGGATCAACGGATAACGCTGCCGCCAGCTCCTTCGCGCTAGTCATCCCCACCGCCAGTTTGCGCCCCCAGCTATAGCGCGTCGCCCACAGCCACTGTTCGTTATCACGCCAGGCCAGACATTGCTGATGAGAAGGTAAAAAAGGCAGGAATCGCTGTAATGCACTGGCATCCGGGGTGATCGCGCTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCG  >  NC_000913/3521770‑3522350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑