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About Keio Collection

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• Host Cell BW25113 - Whole genomic sequence information

• Cell shape
• Reference:
  • Baba T. et al. (2006) Mol Systems Biol, doi:10.1038/msb4100050.
  • Yamamoto N et al. (2009) Mol Systems Biol, doi:10.1038/msb.2009.92
• : Keio collection (Systematic single-gene knock-out mutants of E. coli K-12)   (GenoBase)
• : linked to the PEC ( Profiling of E.coli Chromosome).

Keio collection will be dispatched with kanamycin resistance cassette in each locus. This will help you to isolate the strain by the antibiotic resistance. Please excise the cassette by the recipient.

Attention!! Gene disruption was not occurred in 25 strains in the Keio collection

Attention!! Gene disruption was not occurred in 25 strains in the Keio collection, which was reported in
Yamamoto N et al. (2009) Mol Systems Biol, doi:10.1038/msb.2009.92.
List of the strains was following.
Undisrupted strains

We have noticed that JW2409(ptsI) strains of KEIO collection had a problem. We have replaced with correct one. (November, 2009)

non-commercial research use

Regarding Distribution of Keio collection, ASKA clones, and Gateway entry Clone library
Keio collection, ASKA clones, and Gateway entry clone library will be available only for academic research at non-profit organizations.