Cloning Vector - About

A collection of cloning vectors of Escherichia coli is kept at the National Institute of Genetics as a resource for the biological research community. Only those vectors that can be kept stably as DNA are collected. Therefore, we do not keep lambda phage vectors. Shuttle vectors are also not included because we don't have the facilities to verify their growth in organisms other than E. coli. Vectors are kept as purified DNA.

We accept requests for vectors provided that they are used for basic research. You can browse the catalog of our Collection and order vectors from this web site.

About the Catalog {Download: CloningVectorCollection.pdf(PDF: 47MB)}

Vectors are listed in alphabetical order. The following are brief explanations for each data field.Name: Name of the cloning vector.

Name	Explanation
Name	vector name
Replicon	The origin of replication unit. The pBR322 replicon is designated as pMB1.
Size	The size of vector DNA expressed in Kb. The three decimal values indicate that the total number of nucleotides is known or deducible from the sequence data.
Purpose	The purpose for which the vector was originally constructed.
Selectable Markers	The following abbreviations are used for antibiotic and drug resistance markers. amp ampicillin cml chloramphenicol gen gentamycin hyg hygromycin kan kanamycin mer mercury (II) chloride spc spectinomycin str streptomycin tet tetracycline tmp trimethoprim
Other Genes	Genes other than replication genes and selectable marker genes.
Promoter	Promoter that is located for a specific purpose, such as expression of cloned genes.
Cloning Sites	Cloning sites suitable for vector use.
Reference	Reference literature.
Source	Person from whom the vector was obtained.
Map	MCS stands for Multi Cloning Site. Only relevant restriction sites are indicated. Map is not given if vector information is insufficient for drawing.
Sequence	Accession number for the International DNA Databases (DDBJ, EMBL, and GenBank) or URL of sites where the sequence is made public. Sequences of some vectors are available as printed or electronic data.