Notice: Suspension of Services Due to the Inspection work of NIG

For the inspection work of the institute, services on our website will be stopped during the period of time stated below. We apologize for the inconvenience and thank you for your understanding in this matter.

Fri. 12th Nov. 2021 16:00 - Sun. 14th Nov. 13:00 (JST)

TSS

Name Description Request
1ml of TSS (approximately 10 uses) Transformation solution for iVEC.
Composition of 1ml of TSS (approximately 10 uses)
0.5 ml L broth
0.4 ml 2xTSS
0.1 ml DMSO
Store at 4℃
The composition of L broth and 2x TSS is as follows.
Composition of L broth (100 ml) Composition of 2xTSS (10 ml)
1g Bacto Triptone
0.5 g Bacto Yeast Extract
0.5 g NaCl
Adjust to 100 ml with H2O
Adjust pH to 7 with NaOH
Autoclave at 120℃ for 15 minutes
2g PEG8000
1ml 1M MgSO4
2.5ml 80% glycerol
Adjust to 10ml with L broth
Autoclave at 120℃ for 15 minutes
Mix thoroughly before use
Store at 4℃
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