Gateway Entry Clone library - About

About Gateway Entry Clone library

Details

Gateway entiry clone library of E. coli ORFs

What is Gateway?

Gateway technology is a cloning method using site-specific recombinase and recombination sites (att sites) of phage lambda. Cloning from entry vector to destination vector is easily done by restriction enzyme-free recombination.
Gateway cloning related products can be purchased from Thermo fisher Scientific.
http://www.thermofisher.com/jp/ja/home/life-science/cloning/gateway-cloning.html

Utility

Transfer of the ORFs to other vectors, which was troublesome in ASKA Clone, can be easily done using this library.
It is usable in many applications by introducing to the destination vectors of your interest.
  • Exchange of vectors
  • Exchage of promoters
  • Expression of proteins
  • Addtion of various tags
  • Expression in other species
    • etc.

Deposited by Dr. H. Mori (NAIST)

non-commercial research use

Regarding Distribution of Keio collection, ASKA clones, and Gateway entry Clone library
Keio collection, ASKA clones, and Gateway entry clone library will be available only for academic research at non-profit organizations.

Contact information for Commercial User
1. Request for New Distribution
    If commercial institutions wish to use the materials, or non-profit
    institutions wish to use the materials for purposes other than
    non-commercial research, please contact as follows:
    Research Center for Thermotolerant Microbial Resources (RCTMR),
    Yamaguchi University
    e-mail:e-mail:
    website: https://www.yamaguchi-u.ac.jp/yurctmr/en/index.html
    ＊ Yamaguchi University has received permission from the collection's
        right holders to distribute the collection to third parties.

2. Change of Use to Commercial Purposes
    If you wish to use the materials that have already been provided
    from NBRP for purposes other than non-commercial research, please
    contact as follows:
    Nara Institute of Science and Technology
    Industry-Government-Academia Collaboration
    email:
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