| Notice: Suspension of Services Due to the Inspection work of NIG |
|---|
|
For the inspection work of the institute, services on our website will be stopped during the period of time stated below. We apologize for the inconvenience and thank you for your understanding in this matter. Fri. 12th Nov. 2021 16:00 - Sun. 14th Nov. 13:00 (JST) |
Gateway Entry Clone library - About
About Gateway Entry Clone library
- Reference: Rajagopala S. V. et al. (2010) BMC Genomics, doi: 10.1186/1471-2164-11-470
-
:
Gateway entry clone
(GenoBase) -
: linked to the PEC(Profiling of E.coli Chromosome).
Details
Gateway entiry clone library of E. coli ORFs
What is Gateway?
Gateway technology is a cloning method using site-specific recombinase and recombination sites (att sites) of phage lambda. Cloning from entry vector to destination vector is easily done by restriction enzyme-free recombination.Gateway cloning related products can be purchased from Thermo fisher Scientific.
http://www.thermofisher.com/jp/ja/home/life-science/cloning/gateway-cloning.html
Utility
Transfer of the ORFs to other vectors, which was troublesome in ASKA Clone, can be easily done using this library.It is usable in many applications by introducing to the destination vectors of your interest.
- Exchange of vectors
- Exchage of promoters
- Expression of proteins
- Addtion of various tags
- Expression in other species etc.
Deposited by Dr. H. Mori (NAIST)
