Keio Collection - Cell Shape
|Long and Lysed||1|
|Long and Short||2|
1) Cells were inoculated in L broth containing Kan in 96 well plate overnight at 30℃.
2) Cells were diluted in L broth containing Kan and grown for 2 hours at 37℃.
3) Cells were harvested and resuspended.
4) Cells were spotted on poly-lysine coated slide glass,fixed in methanol and washed with H2O.
5) Cells were stained with DAPI and then observed.