Keio コレクション - Cell Shape
| Cell Shape | Strains | |
|---|---|---|
| Normal |
 |
3535 |
| Chain |
 |
6 |
| Fat |
 |
54 |
| Long |
 |
153 |
| Long and Lysed |
 |
1 |
| Long and Short |
 |
2 |
| Round |
 |
1 |
| Short |
 |
57 |
| Lysed |
 |
3 |
| Very long |
 |
21 |
|
Procedures 1) Cells were inoculated in L broth containing Kan in 96 well plate overnight at 30℃. 2) Cells were diluted in L broth containing Kan and grown for 2 hours at 37℃. 3) Cells were harvested and resuspended. 4) Cells were spotted on poly-lysine coated slide glass,fixed in methanol and washed with H2O. 5) Cells were stained with DAPI and then observed. |
