Keio Collection - Cell Shape

Cell Shape Strains
Normal 3535
Chain 6
Fat 54
Long 153
Long and Lysed 1
Long and Short 2
Round 1
Short 57
Lysed 3
Very long 21

Procedures

1) Cells were inoculated in L broth containing Kan in 96 well plate overnight at 30℃.

2) Cells were diluted in L broth containing Kan and grown for 2 hours at 37℃.

3) Cells were harvested and resuspended.

4) Cells were spotted on poly-lysine coated slide glass,fixed in methanol and washed with H2O.

5) Cells were stained with DAPI and then observed.

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