NIG Wide Deletion Strain Collection (NEDO project) - About
About NIG Wide Deletion Strain Collection (NEDO project)
Although NIG Wide Deletion Strain Collection (NEDO project) in the reference paper has genotype of [ΔrecBCD::Plac-bet exo kan], this portion is restored to wild-type in the strains provided by NBRP E. coli.
It was reported that there are some variations of rpoS sequences in the stocks of W3110.
Jishage M and Ishihama A, J Bacteriol., 179, 959-963 (1997)
In NIG Wide Deletion Strain Collection (NEDO project), the mutations in rpoS gene is as follows.
Leu25(TTG)-->Phe(TTT)
Gln33(CAG)-->Leu(TTG)
It was reported that there are some variations of rpoS sequences in the stocks of W3110.
Jishage M and Ishihama A, J Bacteriol., 179, 959-963 (1997)
In NIG Wide Deletion Strain Collection (NEDO project), the mutations in rpoS gene is as follows.
Leu25(TTG)-->Phe(TTT)
Gln33(CAG)-->Leu(TTG)
