ASKA Clone - About
About ASKA Clone
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Host Cell AG1(ME5305)ASKA clone was made on the basis of the sequence of W3110 genome (GenBank ID: AP009048.1). Therefore, some genes are different from that of MG1655.
AG1, the host strain of ASKA library has auxotrophy for thiamine and histidine. Please add 1 μg/ml of thiamine and 40 μg/ml of histidine into the medium, when you use minimal medium.
- pCA24N: pCA24N.htm
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Reference:
Kitagawa, M et al. (2005) DNA Res. 12 291-299.
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ASKA library (A complete Set of E.coli K-12 ORF Archive)(GenoBase) -
: linked to the PEC(Profiling of E.coli Chromosome).
AG1(pCA24N, -gfp) Additional Information
GFP gene is removed by Notl digestion followed by self-ligation.DNA Research 12, 291-299(2005)
non-commercial research use
Regarding Distribution of Keio collection, ASKA clones, and Gateway entry Clone libraryKeio collection, ASKA clones, and Gateway entry clone library will be available only for academic research at non-profit organizations.
Contact information for Commercial User
1. Request for New Distribution
If commercial institutions wish to use the materials, or non-profit
institutions wish to use the materials for purposes other than
non-commercial research, please contact as follows:
Research Center for Thermotolerant Microbial Resources (RCTMR),
Yamaguchi University
e-mail:e-mail:
website: https://www.yamaguchi-u.ac.jp/yurctmr/en/index.html
＊ Yamaguchi University has received permission from the collection's
right holders to distribute the collection to third parties.
2. Change of Use to Commercial Purposes
If you wish to use the materials that have already been provided
from NBRP for purposes other than non-commercial research, please
contact as follows:
Nara Institute of Science and Technology
Industry-Government-Academia Collaboration
email:
