Notice: Suspension of Services Due to the Inspection work of NIG

For the inspection work of the institute, services on our website will be stopped during the period of time stated below. We apologize for the inconvenience and thank you for your understanding in this matter.

Fri. 12th Nov. 2021 16:00 - Sun. 14th Nov. 13:00 (JST)

ASKA Clone - About

About ASKA Clone

  • Host Cell  AG1(ME5305)
    ASKA clone was made on the basis of the sequence of W3110 genome (GenBank ID: AP009048.1). Therefore, some genes are different from that of MG1655.
    AG1, the host strain of ASKA library has auxotrophy for thiamine and histidine. Please add 1 μg/ml of thiamine and 40 μg/ml of histidine into the medium, when you use minimal medium.

AG1(pCA24N, -gfp) Additional Information

GFP gene is removed by Notl digestion followed by self-ligation.
DNA Research 12, 291-299(2005)

License Agreement Non-Commercial Use of GFP

Please read "License Agreement Non-Commercial Use of GFP".

For those who are going to request ASKA clones GFP (+), We are only able to distribute ASKA clones GFP (+) to Non-commercial users.
This is due to the licencing contract on GFP by GE Healthcare Ltd..
Please contact below if you do not belong to non-commercial organisations.

NIG INNOVATION, National Institute of Genetics
Tel: +81(0)55-981-5835
FAX: +81(0)55-981-5832
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non-commercial research use

NBRP distributes Keio collection and ASKA clones (GFP-) to Non-commercial users.
Please contact below if you do not belong to non-commercial organizations.

Center for Industry-Government-Academia Collaboration, Nara Institute of Science and Technology
Tel: +81-743-72-5191
FAX: +81-743-72-5194
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