ASKA Clone - About
About ASKA Clone
Host Cell AG1(ME5305)ASKA clone was made on the basis of the sequence of W3110 genome (GenBank ID: AP009048.1). Therefore, some genes are different from that of MG1655.
AG1, the host strain of ASKA library has auxotrophy for thiamine and histidine. Please add 1 μg/ml of thiamine and 40 μg/ml of histidine into the medium, when you use minimal medium.
- pCA24N: pCA24N.htm
Kitagawa, M et al. (2005) DNA Res. 12 291-299.
- : ASKA library (A complete Set of E.coli K-12 ORF Archive)(GenoBase)
- : linked to the PEC(Profiling of E.coli Chromosome).
AG1(pCA24N, -gfp) Additional InformationGFP gene is removed by Notl digestion followed by self-ligation.
DNA Research 12, 291-299(2005)
License Agreement Non-Commercial Use of GFPPlease read "License Agreement Non-Commercial Use of GFP".
For those who are going to request ASKA clones GFP (+), We are only able to distribute ASKA clones GFP (+) to Non-commercial users.
This is due to the licencing contract on GFP by GE Healthcare Ltd..
Please contact below if you do not belong to non-commercial organisations.
NIG INNOVATION, National Institute of Genetics
non-commercial research useNBRP distributes Keio collection and ASKA clones (GFP-) to Non-commercial users.
Please contact below if you do not belong to non-commercial organizations.
Center for Industry-Government-Academia Collaboration, Nara Institute of Science and Technology