ホーム > Keio コレクション > 全ゲノム配列情報

Keio コレクション - 全ゲノム配列情報

BW25113株の全ゲノムをSolexaシークエンサー（イルミナ）によって解読しました。
解読したゲノムは、大腸菌W3110のゲノム配列（アクセションナンバー AP009048）と比較しました。
W3110と異なる箇所が４０カ所見出されました。そのうち、BW25113では欠失している配列が１８カ所、
挿入配列がある場所が４カ所、ミスマッチ変異が１８カ所見出されました。挿入配列、ミスマッチ変異の
箇所に関しては、その領域をPCRで増幅し、シークエンスで確認しました。
文責 塩見 大輔（仁木研究室）

• Differences between BW25113 and W3110
  AP009048 (W3110) 4646332 bp
  *Number shows location in genome of W3110 (AP009048)
  

(1) regions deleted (18)

Start - End	Gene	Confirmation by PCR and sequencing after the whole genomic sequencing	Note
66,533 - 70,072	araBAD		predicted from genotype of BW25113
363,580 - 365,642	lacZ		predicted from genotype of BW25113
654,214 - 655,407	IS (insH)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
1,105,622 - 1,106,951	IS (insD)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
1,301,041 - 1,302,369	IS (insC)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
1,980,209 - 1,981,402	IS (insH)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
2,176,983 - 2,178,176	IS (insH)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
2,320,795 - 2,322,124	IS (insC)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
2,411,459 - 2,412,225	IS (insB)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
2,526,403 - 2,526,947	valUXY, lysV		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
3,261,134 - 3,262,328	IS (insH)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
3,539,230 - 3,542,690	rhaBAD		predicted from genotype of BW25113
3,742,936 - 3,751,785			reported as difference between W3110 and MG1655 by Hayashi et al., 2006
(3,742,936 - 3,744,263)	IS (insDC)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
(3,746,911 - 3,748,105)	IS		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
(3,750,591 - 3,751,785)	IS (insH)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
4,310,363 - 4,311,557	IS (insH)		reported as difference between W3110 and MG1655 by Hayashi et al., 2006
4,371,274 - 4,371,275	dcuA frameshift		reported as difference between W3110 and MG1655 by Hayashi et al., 2006

(2) regions inserted (4)

Position	Gene	Confirmation by PCR and sequencing after the whole genomic sequencing	Note
around 370,970	mhpC	not yet confirmed because of failure of PCR
547,835 - 547,836 G was inserted	ylbE	confirmed
around 2,564,140	eutB-eutA	not yet confirmed because of failure of PCR	reported as difference between W3110 and MG1655 by Hayashi et al., 2006
around 4,215,890	rrlE	not yet confirmed because of failure of PCR

(3)Mismatch (18)

Position	Gene	Confirmation by PCR and sequencing after the whole genomic sequencing	Note
70,289 T	between araB and araC	not yet confirmed because of failure of PCR
365,743 ?	lacI	not yet confirmed because of failure of PCR
502,653 T	between ybaL and fsr	C-->T, confirmed
547,694 G	ylbE	A-->G, confirmed
705,435 C	nagE	T-->C, confirmed
1,093,686 C	ycdT	T-->C, confirmed	reported as difference between W3110 and MG1655 by Hayashi et al., 2006
2,034,560 G	yedJ	A-->G, confirmed	reported as difference between W3110 and MG1655 by Hayashi et al., 2006
2,842,666 T	hycG	G-->T, confirmed
2,866,111 G	between rpoS and nlpD	A-->G, confirmed
3,109,077 A	pheV (tRNA Phe)	C-->A, confirmed
3,472,558 C	btuB	G-->C, confirmed
3,475,433 A	fabR	C-->A, confirmed
3,542,911 A	rhaD	not yet confirmed because of failure of PCR
4,154,211 G	crp	T-->G, confirmed	reported as difference between W3110 and MG1655 by Hayashi et al., 2006
4,479,514 G	yjgJ	A-->G, confirmed
4,590,160 A	hsdR	G-->A, confirmed	predicted from genotype of BW25113
4,607,189 T	yjjP	C-->T, confirmed
4,621,349 T	yjjI	G-->A, confirmed

Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21.