Update information

• May. 12 2026
• ME Collection was added.(2 strains)
• Jan. 7 2026
• ME Collection was added.(4 strains)
• Aug. 25 2025
• Released and updated the genome information of the following strains.
• Gene Mutants Strains：158 strains
• Large-scale chromosomal deletionmutant：17 strains
• NIG Wide Deletion Strain Collection：33 strains
• Cloning Vector：73 strains
• Aug. 22 2025
• ME Collection was added.(3 strains)
• Apr. 1 2025
• From April 1, 2025 onwards, the fees about bio-resources were modified.
• Mar. 18 2025
• From April 1, 2025 onwards, the fees about bio-resources will be modified.
• Dec. 19 2024
• The name of the KHK collection was changed to the NIG Wide Deletion Strain Collection (NEDO project).
• Aug. 22 2024
• ME Collection was added.(11 strains)
• Jun. 10 2024
• ME Collection was added.(76 strains)
• May. 27 2024
• To ensure the quality of the resource and improve its quality and additional value, we analyzed the nucleotide sequences of Cloning Vector and released the complete nucleotide sequences of 191 plasmids.
• Mar. 1 2024
• To ensure the quality of the resource and improve its quality and additional value, we analyzed the whole genome sequences of major strains and released the genome information of the following 48 strains.
• • Gene Mutants Strains：23 strains
  • Large-scale chromosomal deletionmutant：17 strains
  • Transposon insertion disruptant Host Cell
  • Cloning Vector：7 strains
• Gene Disrupted Strains of metabolism-related enzymes can easily be selected from a list of pathway names.
• Feb. 6 2024
• ME Collection was added.(6 strains)
• Dec. 19 2023
• Antibody Collection was added.(3 antibodies)
• Sep. 7 2023
• ME Collection was added.(42 strains)
• Apr. 26 2023
• ME Collection was added.(45 strains)
  Cloning Vector Collection was added.(108 vectors)
• Mar. 16 2023
• Antibody Collection was added.(16 antibodies)
• Jan. 26 2023
• ME Collection was added.(2 strains)
• Nov. 11 2022
• Genome maps of 20 IH mutant strains were released.
• Jul. 13 2022
• Genetic marker-less Genome editing tool has been released.
• Jan. 21 2022
• ME Collection was added.(1 strain)
• Nov. 5 2019
• We published the instruction for iVEC express.
• Oct. 2 2019
• ME Collection was added.(2 strains)
• Jun. 10 2019
• ME Collection was added.(9 strains)
• May 15 2019
• ME Collection was added.(10 strains)
• Jul. 23 2018
• Glycerol stocks of whole set of Keio Collection ,ASKA Clone,Gateway Entry Clone library and Mobile plasmid Collection are now available.
• Jun. 13 2018
• ME Collection was added.(67 strains)
• Jun. 11 2018
• Bio-resource fees were modified.
  Refer to Price List for further details.
• Jun. 8 2018
• We are currently undergoing maintenance and cannot distribute any resources.
  We will resume our services on Monday 11 June 2018.
• Apr. 11 2018
• Genome information for ME8077 and ME8162 was added.
• Apr. 10 2018
• ME Collection was added.(5 strains)
• Nov. 20 2017
• The edit account functionality was changed.
  The name of your account can only be edited during new registration. You are required to create a new account if you want to change the name of your account.
• Sep. 12 2017
• With MTA Form 1, the MTA can now be concluded online.
• Jul. 28 2017
• ME Collection was added.(1 strain)
• May 26 2017
• ME Collection was added.(22 strains)
• Oct. 31 2016
• ME Collection was added.(1 strain)
• TSS was started.
• Jun. 23 2016
• ME Collection was added.(2 strains)
• Jun. 1 2016
• Institution related fields in account information were modified. If you want to choose high school, select "Other educational institution" from the list.
• May 13 2016
• ME Collection was added.(1 strain)
• Apr. 2016
• We sequenced the whole genome of ten E. coli strains and ten Cloning vectors.
  Please see here for information of the strains (vector names) and the accession numbers.
• Mar. 30, 2016
• Revamp of the NBRP E.coli Strain website.
• Dec. 7, 2015
• We have revealed that histidine, in addition to thiamine, is required for the growth of ASKA library in minimal medium.
• Sep. 17, 2015
• ME Collection was added.(84 strains)
• Jan. 23, 2014
• MECollection was added.(37 strains)
• Jun. 24, 2013
• Antibody Collection Request was started.
• Oct. 19, 2012
• ME Collection was added.(8 strains)
• Sep. 28, 2012
• NIG Wide Deletion Strain Collection (NEDO project) Request was started.
• Apr. 20, 2012
• Large-scale chromosomal deletion mutants (Tokyo Metropolitan University Group) was added.(35 strains)
• Jan. 14, 2011
• Phage was added.(142 strains)
• Oct. 18, 2010
• "Please acknowledge us!" page was added.
• Sep. 6, 2010
• Transposon insertion disruptant (102 strains) was deleted.
• Transposon insertion disruptant (6227 strains) and Transposon insertion disruptant (cis partial doublet strain) (3 strains) was corrected.
• Aug. 23, 2010
• Transposon insertion disruptant (cis partial doublet strain) was added.(866 strains)
• May 10, 2010
• 4 Cosmid Collection strains were deleted (E2324, E4053, E4337, E2210).
• Apr. 1, 2010
• A fee for strains will be charged.
  Refer to Shipment and Cost for further details.
• Jan. 4, 2010
• Large-scale chromosomal deletion mutants (Tokyo Metropolitan University Group) was added.(448 strains)
• Nov. 2009
• We have noticed that JW2409(ptsI) strains of KEIO collection had a problem. We have replaced with correct one.
• Aug. 25, 2009
• IH E.coli Mutant Collection was added.(102 strains)
• "Shipment and Cost" page and "Upon publication…" page were added.
• Aug. 3, 2009
• IH E.coli Mutant Collection was added.(111 strains)
• Jan. 2009
• 550#15(ORF No.(Japan)) of the Mobile Plasmid Collection was deleted.
• Dec. 4, 2008
• AG1(pCA24N, -gfp) and AG1(pCA24N)were added, and the request was started.
• ME Collection(6 strains), JE Collection(235 strains) was deleted.
• "Order All" buttons were added to Cosmid Collection.
• Flow of application was added.
• MTA Form1 sample was added.
• Jun. 1, 2008
• ASKA Clone(+) was added.
• Request of the pLC Plasmid Collection and Cosmid Collection was added.
• The following pages were added, and corrected.
• • ASKA Clone Browse & Search
  • Mobile plasmid Collection Browse & Search
  • Cosmid Collection Search
  • pLC Plasmid Browse & Search
  • Transposon insertion disruptant Browse & Search
• Apr. 1 2008
• Cloning Vector Collection was added.
• Apr. 5, 2007
• Tracking System was added.
• ASKA Clone Search & Browse and Keio Collection Search & Browsewas added.
• Jan. 15, 2007
• The result of feedback was added.
• Jan. 12, 2007
• Explanation of "Scheme of order and distribution of the KEIO/ASKA strains" was added.
• Nov. 14, 2006
• The host stock BW25113 of ASKA Clone(-) and Keio Collection was added.
• Nov. 2, 2006
• Request of the host cell KP7600 of Transposon insertion disruptant was added.
• Sep. 5, 2006
• The automatic input of the approval form was added.
• Aug. 24, 2006
• Keio Collection Request was started.
• Aug. 10, 2006日
• ASKA clone(-) is available!
• Jul. 28, 2006
• "Order All" buttons were added to Mobile Plasmid Collection , Large-scale chromosomal deletion mutants (Tokyo Metropolitan University Group) , Transposon insertion disruptant.
• Jun. 1, 2006
• Transposon insertion disruptant data was added.
  (JD20095, JD20548, JD23952, JD24006, JD24345, JD25076, JD25987)
• May 26, 2006
• Gene Mutants and Mobile Plasmid Collection and Transposon insertion disruptant data were updated.
• May 23, 2006
• Resource Type was changed.
• Apr. 4, 2006
• Cloning Vector imformation was added.
• Jan. 24, 2006
• Transposon insertion disruptant data was updated.
• Nov. 10, 2005
• Transposon insertion disruptant was added.
• The composition of page was updated.
• May 10, 2005
• These deletion mutants will temporarily be distributed by "Nara Institute of Science and Technology".
• Apr. 7, 2005
• The Large-scale chromosomal deletion mutants (Tokyo Metropolitan University Group)are mutually linked to the PEC (Profiling of E.coli Chromosome).
• Deletion Mutants are mutually linked to the PEC (Profiling of E.coli Chromosome).
• Mar. 4, 2005
• The cart system was added to the following data.
• • Mobile Plasmid Clone
  • Deletion Mutants
  • Large-scale chromosomal deletion mutants (Tokyo Metropolitan University Group)
• Download was updated.
• How to Request was updated.
• Affiliation was added to the user information on the request.
• Feb. 10, 2005
• The strain informations was corrected.（2 new strains, 15 strains were updated, 1 strain was deleted.)
• Feb. 3, 2005
• Marker List was added.
• Deletion Mutants added the link to GenoBase.
• Dec. 3, 2004
• The chromosomal markers of strains are mutually linked to the E.coli Genome Database (PEC).
• Nov. 11, 2004
• Large-scale chromosomal deletion mutants (Tokyo Metropolitan University Group) was added to Resource Type. (126 deletions)
• Aug. 10, 2004
• The strain informations was corrected. ( 6 strains ）
• Aug. 2, 2004
• The menu was changed.
• The data of pLC Plasmids was changed.
• Jul. 27, 2004
• The following information was added to Resource Type.
• • Culture Condition
  • Mobile plasmid clone
  • Deletion Mutants
• Jun. 14, 2004
• NBRP Strains Request is available!