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Summary
| Resource Type | Hit Count |
|---|---|
| Gene Mutants | 4hits |
| Culture Condition | 0hits |
| ASKA Clone(-) | 1hits |
| ASKA Clone(+) | 1hits |
| Gateway Entry Clone library | 1hits |
| Mobile Plasmid Clone | 1hits |
| Cosmid Collection | 0hits |
| pLC Plasmid Collection | 0hits |
| Keio Collection | 1hits |
| Large-scale chromosomal deletion mutant (Tokyo Metropolitan University Group) | 0hits |
| NIG Wide Deletion Strain Collection (NEDO project) | 0hits |
| Transposon insertion disruptant | 0hits |
| Transposon insertion disruptant (cis partial doublet strain) | 1hits |
| Phage | 0hits |
| Cloning Vector Collection | 0hits |
| Cloning Vector CollectionHost Cell | 0hits |
| Antibody | 0hits |
Gene Mutants - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
4hits
First Previous 1-4 Next Last All| ME No. | Strain Name | Sex | Genotype | Other Remarks | Gene Mutants Detail Info | Request |
|---|---|---|---|---|---|---|
| IH48 | W3110ΔmiaA | F- | miaA |
(*)in viro nene manupulation -> transformation to JC7623 -> P1 transduction.
|
||
| IH49 | W3110ΔtRNALeu6ΔmiaA | F- | leuX miaA |
|
||
| IH95 | BT63 ts29ΔssrA | F- | miaA ΔssrA supP lac1000 trpam metam3 strr bfeam tsxam |
The ts29 phenotype is complementated by introduction of the wild-type leuX and Kohara clone #652 (miaA+)
|
 Reference Genome: MG1655 (Accession No. NC_000913) |
|
| IH52 | BT63 ts29 | F- | miaA supP lac1000 trpam metam3 strr bfeam tsxam |
(*)NTG-mutagenesis and penicillin-screening.
|
ASKA Clone(-) - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
1hits
First Previous 1-1 Next Last All
Resource No (JW ID)
 |
Gene Name
|
Primer Sequence for N terminal | Primer Sequence for C terminal | Comment | Request |
|---|---|---|---|---|---|
| JW4129-AM | miaA | GCCAGTGATATCAGTAAGGCGAG | CCGCCTGCGATAGCACCAACAAC | Clone OK |
ASKA Clone(+) - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
1hits
First Previous 1-1 Next Last All|
Resource No (JW ID)
|
Gene Name
|
Primer Sequence for N terminal | Primer Sequence for C terminal | Comment | Request |
|---|---|---|---|---|---|
| JW4129-AP | miaA | GCCAGTGATATCAGTAAGGCGAG | CCGCCTGCGATAGCACCAACAAC | Clone OK |
Gateway Entry Clone library - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
1hits
First Previous 1-1 Next Last All|
Resource No (JW ID)
|
Gene Name
|
Clone verification class | Cloning Method | Request |
|---|---|---|---|---|
| JW4129-GE | miaA | A | ASKA to pDONR/zeo |
Mobile Plasmid Collection - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
1hits
First Previous 1-1 Next Last All| Accession No | ORF No.(Japan) | ORF No.(USA) | Gene Name | Length | Source | Plasmid | Request |
|---|---|---|---|---|---|---|---|
| 3998 | 652#8 | b4171 | miaA | 951 | pNTR-SD |
Keio Collection - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
1hits
First Previous 1-1 Next Last All|
Resource No (JW ID)
|
Gene Name
|
Comment |
Cell Shape |
Cell Shape Image | Request |
|---|---|---|---|---|---|
| JW4129-KC | miaA | ready to distribute | Normal |
 |
Transposon insertion disruptant (cis partial doublet strain) - List
Search Condition : (Keyword = miaA b4171 ECK4167 JW4129 trpX)
1hits
First Previous 1-1 Next Last All| Strain No | Insertion site (inserted after) |
MG1655 Gene name |
Request |
|---|---|---|---|
| JD17830 | 4397997 | miaA |
