breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
RA 113,438 C→T intergenic (+150/‑6) yacM → / → guaC protein YacM/GMP reductase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC 279,931 Δ11,471 bp between IS1 yagBinsA3 yagB, yagA, yagE, yagF, yagG, yagH, xynR, argF, argL, ykgS, insB3, insA3
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 334,188 G→A I82I (ATC→ATT yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
JC JC 362,107 IS5 (+) +4 bp coding (1070‑1073/1254 nt) lacY ← lactose permease
RA 466,169 T→A T382S (ACC→TCC)  ybaE ← uncharacterized protein YbaE
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 631,618 Δ1 bp coding (1725/2106 nt) cstA → pyruvate transporter CstA
MC JC 678,921 Δ8,930 bp IS5‑mediated [ybeT]gltI [ybeT], ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
JC JC 792,077 IS2 (+) +5 bp intergenic (‑22/+235) galE ← / ← modF UDP‑glucose 4‑epimerase/ABC family protein ModF
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
JC JC 1,217,464 IS2 (–) +5 bp coding (137‑141/1524 nt) pdeG → putative c‑di‑GMP phosphodiesterase PdeG
RA 1,290,644 G→T E135* (GAA→TAA)  rssB → regulator of RpoS
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,381,704 A→C intergenic (+897/‑243) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,383,033 A→T L310* (TTA→TAA)  ycjW ← DNA‑binding transcriptional repressor YcjW
RA 1,449,061 T→G intergenic (+43/+15) feaB → / ← tynA phenylacetaldehyde dehydrogenase/copper‑containing amine oxidase
RA 1,484,291 C→T L411F (CTC→TTC)  hrpA → ATP‑dependent 3'‑>5' RNA helicase HrpA
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 1,656,167 IS2 (–) +5 bp intergenic (+18/‑13) ynfB → / → speG DUF1283 domain‑containing protein YnfB/spermidine N‑acetyltransferase
JC JC 1,671,703 IS2 (–) +5 bp intergenic (+19/‑70) asr → / → ydgU periplasmic chaperone Asr/uncharacterized protein YdgU
RA 1,703,430 C→T L293L (TTG→TTA ydgJ ← putative oxidoreductase YdgJ
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
JC JC 1,743,490 IS5 (+) +4 bp coding (34‑37/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,856,968 T→A I268F (ATT→TTT)  ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,096,437 Δ1 bp coding (594/777 nt) hisF → imidazole glycerol phosphate synthase subunit HisF
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,239,760 C→T E197K (GAA→AAA)  mglB ← D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
JC JC 2,452,057 IS2 (–) +5 bp coding (276‑280/753 nt) yfcS ← putative fimbrial chaperone YfcS
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
JC JC 2,788,791 IS5 (–) +4 bp intergenic (‑2954/‑191) ileY ← / → glaH tRNA‑Ile/glutarate dioxygenase GlaH
MC JC 2,867,280 Δ8 bp coding (265‑272/993 nt) rpoS ← RNA polymerase sigma factor RpoS
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
JC JC 3,067,128 IS5 (–) +4 bp coding (43‑46/897 nt) ygfI ← putative DNA‑binding transcriptional regulator YgfI
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,101,039 (C)7→8 coding (585/720 nt) yggN ← DUF2884 domain‑containing protein YggN
JC JC 3,136,515 IS5 (+) +4 bp intergenic (‑144/+145) pitB ← / ← gss metal phosphate:H(+) symporter PitB/fused glutathionylspermidine amidase/glutathionylspermidine synthetase
RA 3,263,231 T→A T144S (ACC→TCC)  tdcD ← propionate kinase
RA 3,308,048 G→A Q293* (CAG→TAG)  nlpI ← lipoprotein NlpI
RA 3,316,428 C→T E367K (GAA→AAA)  nusA ← transcription termination/antitermination protein NusA
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
JC JC 3,367,057 IS2 (–) +5 bp coding (132‑136/717 nt) yhcF → DUF1120 domain‑containing protein YhcF
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
JC JC 3,437,349 IS30 (–) +2 bp coding (832‑833/1377 nt) trkA → NAD‑binding component of Trk potassium transporters
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,475,993 T→C T150A (ACC→GCC)  yheO ← DNA‑binding transcriptional regulator YheO
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,566,550 G→A T11M (ACG→ATG)  glgP ← glycogen phosphorylase
RA 3,629,921 C→T E228K (GAG→AAG)  yhiI ← putative membrane fusion protein YhiI
JC JC 3,638,509 IS5 (–) +4 bp coding (868‑871/1500 nt) pitA → metal phosphate:H(+) symporter PitA
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
MC JC 3,708,467 Δ168 bp [proK] [proK]
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,761,230 G→A S7F (TCC→TTC)  selA ← selenocysteine synthase
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,912,074 G→A S532F (TCC→TTC)  glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
RA 3,926,183 G→A G91G (GGC→GGT mioC ← flavoprotein MioC
RA 3,926,210 T→C A82A (GCA→GCG mioC ← flavoprotein MioC
RA 3,926,815 C→T Q63Q (CAG→CAA asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,927,019 G→A intergenic (‑16/‑136) asnC ← / → asnA DNA‑binding transcriptional dual regulator AsnC/asparagine synthetase A
RA 3,927,070 A→T intergenic (‑67/‑85) asnC ← / → asnA DNA‑binding transcriptional dual regulator AsnC/asparagine synthetase A
RA 3,927,184 T→C R10R (CGT→CGC asnA → asparagine synthetase A
RA 3,927,217 T→C R21R (CGT→CGC asnA → asparagine synthetase A
RA 3,927,704 G→T G184W (GGG→TGG)  asnA → asparagine synthetase A
RA 3,927,736 C→T G194G (GGC→GGT asnA → asparagine synthetase A
RA 3,927,877 G→T P241P (CCG→CCT asnA → asparagine synthetase A
RA 3,927,904 C→T S250S (TCC→TCT asnA → asparagine synthetase A
RA 3,927,919 T→C R255R (CGT→CGC asnA → asparagine synthetase A
RA 3,927,922 A→G V256V (GTA→GTG asnA → asparagine synthetase A
RA 3,927,935 C→T L261L (CTG→TTG)  asnA → asparagine synthetase A
RA 3,927,946 2 bp→GT coding (792‑793/993 nt) asnA → asparagine synthetase A
RA 3,927,964 C→T D270D (GAC→GAT asnA → asparagine synthetase A
RA 3,927,973 C→T R273R (CGC→CGT asnA → asparagine synthetase A
RA 3,927,977 G→C E275Q (GAG→CAG)  asnA → asparagine synthetase A
RA 3,927,985 G→A E277E (GAG→GAA asnA → asparagine synthetase A
RA 3,928,057 T→C T301T (ACT→ACC asnA → asparagine synthetase A
RA 3,928,102 A→T G316G (GGA→GGT asnA → asparagine synthetase A
RA 3,930,314 C→T L260L (TTG→TTA ravA ← regulatory ATPase RavA
RA 3,930,650 A→G R148R (CGT→CGC ravA ← regulatory ATPase RavA
MC JC 3,931,455 Δ7,037 bp [kup][rbsR] [kup], rbsD, rbsA, rbsC, rbsB, rbsZ, rbsK, [rbsR]
RA 3,952,642 C→T R53R (CGC→CGT ilvE → branched‑chain‑amino‑acid aminotransferase
RA 3,959,055 T→C I362I (ATT→ATC ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,100 C→T V377V (GTC→GTT ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,115 T→C I382I (ATT→ATC ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,121 G→A E384E (GAG→GAA ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,127 A→T A386A (GCA→GCT ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,130 T→C Y387Y (TAT→TAC ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,148 G→A E393E (GAG→GAA ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,220 T→C A417A (GCT→GCC ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,959,260 T→C L431L (TTG→CTG)  ilvC → ketol‑acid reductoisomerase (NADP(+))
MC JC 3,959,446 55 bp→14 bp intergenic (+1/+32) ilvC → / ← ppiC ketol‑acid reductoisomerase (NADP(+))/peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,538 G→A R92R (CGC→CGT ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,544 C→A L90L (CTG→CTT ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,562 A→G Y84Y (TAT→TAC ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,565 T→A G83G (GGA→GGT ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,577 G→A H79H (CAC→CAT ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,589 G→A T75T (ACC→ACT ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,619 C→T V65V (GTG→GTA ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,730 C→A G28G (GGG→GGT ppiC ← peptidyl‑prolyl cis‑trans isomerase C
RA 3,959,894 T→G intergenic (‑81/‑783) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,959,982 A→G intergenic (‑169/‑695) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,002 T→G intergenic (‑189/‑675) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,114 C→T intergenic (‑301/‑563) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,209 C→T intergenic (‑396/‑468) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,240 T→A intergenic (‑427/‑437) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,251 +A intergenic (‑438/‑426) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,284 G→A intergenic (‑471/‑393) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,483 C→A intergenic (‑670/‑194) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,960,553 G→A intergenic (‑740/‑124) ppiC ← / → rep peptidyl‑prolyl cis‑trans isomerase C/ATP‑dependent DNA helicase Rep
RA 3,961,108 T→C N144N (AAT→AAC rep → ATP‑dependent DNA helicase Rep
RA 3,961,120 A→T T148T (ACA→ACT rep → ATP‑dependent DNA helicase Rep
RA 3,961,124 T→G S150A (TCC→GCG)  rep → ATP‑dependent DNA helicase Rep
RA 3,961,126 C→G S150A (TCC→GCG rep → ATP‑dependent DNA helicase Rep
RA 3,961,135 A→G A153A (GCA→GCG rep → ATP‑dependent DNA helicase Rep
RA 3,961,138 +G coding (462/2022 nt) rep → ATP‑dependent DNA helicase Rep
RA 3,961,141 Δ1 bp coding (465/2022 nt) rep → ATP‑dependent DNA helicase Rep
RA 3,961,144 G→A A156A (GCG→GCA rep → ATP‑dependent DNA helicase Rep
RA 3,961,146 2 bp→AG coding (470‑471/2022 nt) rep → ATP‑dependent DNA helicase Rep
RA 3,961,159 C→T D161D (GAC→GAT rep → ATP‑dependent DNA helicase Rep
RA 3,961,280 A→G K202E (AAG→GAG)  rep → ATP‑dependent DNA helicase Rep
RA 3,961,306 T→C Y210Y (TAT→TAC rep → ATP‑dependent DNA helicase Rep
RA 3,961,351 G→A E225E (GAG→GAA rep → ATP‑dependent DNA helicase Rep
RA 3,961,587 C→T S304F (TCC→TTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,961,980 C→T A435V (GCC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,962,444 G→A D590N (GAT→AAT)  rep → ATP‑dependent DNA helicase Rep
RA 3,962,449 G→A E591E (GAG→GAA rep → ATP‑dependent DNA helicase Rep
RA 3,963,807 T→A G141G (GGA→GGT gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,963,843 A→G G129G (GGT→GGC gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,963,849 G→T T127T (ACC→ACA gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,963,921 A→G I103I (ATT→ATC gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,963,966 G→A R88R (CGC→CGT gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,963,972 C→T T86T (ACG→ACA gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,964,008 A→G I74I (ATT→ATC gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,964,040 A→G L64L (TTG→CTG)  gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,964,071 G→A S53S (TCC→TCT gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,964,074 G→C L52L (CTC→CTG gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,964,361 (T)6→4 intergenic (‑132/+3) gpp ← / ← rhlB guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase/ATP‑dependent RNA helicase RhlB
RA 3,964,422 G→A R403R (CGC→CGT rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,964,425 C→T P402P (CCG→CCA rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,031,457 C→T F99F (TTC→TTT pepQ → Xaa‑Pro dipeptidase
RA 4,031,511 C→T S117S (AGC→AGT pepQ → Xaa‑Pro dipeptidase
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,366,717 (C)6→5 coding (57/1302 nt) dcuA ← C4‑dicarboxylate transporter DcuA
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,499,661 T→C intergenic (+161/‑1599) insD6 → / → yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,518,627 2 bp→AC coding (101‑102/276 nt) insA7 → IS1 family repressor protein InsA
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196304 1211450 15147 38 [32] [32] 34 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 99 [0] [0] 103 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423765–3424234 3424492–3424239 6–728 34 [33] [32] 34 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3941856–3942011 3943210–3942028 18–1355 35 [33] [33] 34 rrsC 16S ribosomal RNA
* * ÷ NC_000913 3943770–3944036 3945384–3944061 26–1615 35 [33] [32] 37 rrlC 23S ribosomal RNA
* * ÷ NC_000913 4640415 4640417 3 107 [0] [0] 132 yjjY uncharacterized protein YjjY

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994982 (0.020)154 (1.600) 89/578 0.0 99.4% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)99 (1.010) 57/586 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 = 1469241NA (NA)114 (1.200)
+GTACGTAA
66/570 0.1 NA intergenic (‑92/‑5) insC2/ydbA IS2 insertion element repressor InsA/putative outer membrane protein, N‑terminal fragment
?NC_000913 1469295 = NA (NA)pseudogene (50/50 nt) ydbA putative outer membrane protein, N‑terminal fragment
* ? NC_000913 1979271 =0 (0.000)103 (1.060) 69/586 0.1 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 2171428NA (NA)81 (0.830) 53/584 0.5 81.8% noncoding (1258/1258 nt) IS3 repeat region
?NC_000913 2176471 = 18 (0.190)coding (734/855 nt) gatY tagatose‑1,6‑bisphosphate aldolase 2
* ? NC_000913 4498181 =NA (NA)48 (0.490) 32/586 1.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/‑1748) insD6/yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
* ? NC_000913 = 4499511NA (NA)107 (1.100) 62/586 0.2 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 4640414 0 (0.000)coding (13/141 nt) yjjY uncharacterized protein YjjY
* ? NC_000913 4507459 =NA (NA)132 (1.360) 82/584 0.0 100% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 4640418 = 0 (0.000)coding (17/141 nt) yjjY uncharacterized protein YjjY