breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 52 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,7470C.76.8% 228.5 / 96.2 99intergenic (+145/+469)yhaC/rnpBuncharacterized protein YhaC/RNase P catalytic RNA component
*NC_000913339,9860GA75.7% 58.3 / 12.8 37R73Q (CGA→CAA) yahHputative uncharacterized protein YahH
*NC_000913339,9790GA75.0% 49.2 / 17.0 37V71M (GTG→ATG) yahHputative uncharacterized protein YahH
*NC_0009132,724,8210C.72.2% 304.7 / 181.2 152coding (926/1299 nt)kgtPalpha‑ketoglutarate:H(+) symporter
*NC_0009131,259,9770CT70.5% 134.2 / 67.4 90R276H (CGC→CAC) dauAaerobic C4‑dicarboxylate transporter DauA
*NC_0009133,875,2980CT63.0% 110.4 / 103.9 108intergenic (‑138/‑140)dgoR/yidXDNA‑binding transcriptional regulator DgoR/putative lipoprotein YidX
*NC_0009132,171,3840AC52.4% 14.8 / 24.8 21K285Q (AAG→CAG) insF5IS3 element protein InsF
*NC_0009133,319,7660CA40.0% 100.3 / 18.2 100S377* (TCA→TAA) argGargininosuccinate synthetase
*NC_0009133,911,5330CA39.2% 155.3 / 19.1 121intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009131,611,5070GT34.6% 130.9 / 15.4 101L116L (CTC→CTAdgcFputative diguanylate cyclase DgcF
*NC_0009133,054,8860GT34.3% 173.3 / 32.1 108Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009131,351,4630CA32.6% 157.6 / 13.0 95E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009131,502,7720GA32.1% 150.0 / 35.2 107A106T (GCG→ACG) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_0009133,054,8940GT32.0% 165.4 / 23.8 100P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009131,631,9030GT31.5% 178.8 / 13.2 108S128Y (TCC→TAC) ydfJputative transporter YdfJ
*NC_0009132,662,4420TA30.9% 61.2 / 43.0 42intergenic (‑311/+141)iscR/trmJDNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase
*NC_00091358,0650CA30.4% 184.3 / 13.1 102P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009131,557,4280GT30.3% 146.3 / 11.1 99S204* (TCG→TAG) ddpFputative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF
*NC_0009132,722,8380GT29.6% 198.7 / 10.4 108E38* (GAG→TAG) pssAphosphatidylserine synthase
*NC_000913314,5940GT29.0% 199.5 / 12.0 107intergenic (‑1352/‑697)ykgR/insE1putative membrane protein YkgR/IS3 element protein InsE

Marginal new junction evidence (lowest skew 10 of 48 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2290113NA (NA)20 (0.200) 16/586 4.4 15.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4002526 = 112 (1.150)coding (274/381 nt) yigF DUF2628 domain‑containing protein YigF
* ? NC_000913 = 460616686 (0.880)11 (0.160) 9/408 4.6 16.0% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 56 (0.820)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 78144665 (0.670)10 (0.140) 9/430 4.8 18.4% intergenic (+2/‑131) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 781655 = 41 (0.570)intergenic (+3/‑430) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 2653419105 (1.080)15 (0.170) 12/536 5.0 14.0% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 88 (0.990)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 2170171 =NA (NA)30 (0.310) 13/582 5.1 19.9% noncoding (1/1258 nt) IS3 repeat region
?NC_000913 2700291 = 121 (1.250)intergenic (‑94/‑229) shoB/ohsC toxic peptide ShoB/small regulatory RNA OhsC
* ? NC_000913 315229 =NA (NA)30 (0.310)
+T
13/584 5.2 19.9% noncoding (1/1255 nt) IS3 repeat region
?NC_000913 2700289 = 121 (1.240)intergenic (‑92/‑231) shoB/ohsC toxic peptide ShoB/small regulatory RNA OhsC
* ? NC_000913 = 69674164 (0.660)8 (0.110) 7/430 5.6 15.1% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 43 (0.600)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 3648146138 (1.410)10 (0.130) 8/470 5.7 8.2% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 114 (1.460)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 294746773 (0.750)6 (0.090) 6/420 5.9 10.1% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 54 (0.770)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 251811672 (0.740)6 (0.080) 6/424 6.0 11.3% noncoding (1/76 nt) alaX tRNA‑Ala
?NC_000913 2518232 = 42 (0.590)intergenic (‑1/‑235) alaW/yfeC tRNA‑Ala/DNA‑binding transcriptional dual regulator YfeC