Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,772	T→C	F236F (TTT→TTC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,772	0	T	C	100.0%	303.9 / NA	79	F236F (TTT→TTC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/33);  total (46/33)

GGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTAC  >  NC_000913/4396478‑4397061                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                   gtttgcgccactcgTTGAACAGCCTGCTGTTTTCGCACCGCTCGGCAGCGCCAAGGCTCGCAATCCTGTTAAACCGGAAAGTAACCGAACTACGGGTGTTATCAGCAGTCATCTGTTGTCGTGTCAGTCGGTTCACGTGGGCGCTAATACTGGCGATAAAAGCATCATCGCTAGTG‑TGTCTGGACGCGGCGGTCAGGACTCTGTCGCTATTAGC‑CCCGGTGGTACGTAGGAGGGAGA‑‑CGTCCCCAGCGCCATCGAGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAgg                                                                                                                                                                                                                                                                                               <  1:373426/288‑1 (MQ=255) ggTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAgg                                                                                                                                                                                                                                                                                               <  2:196386/300‑1 (MQ=255) ggTTGCGAAACGCGGTGAAACGACTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTAGCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTTTCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAgg                                                                                                                                                                                                                                                                                               <  2:197198/300‑1 (MQ=255)  gTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGGACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCNGCAGTAATACGGTAACGCGTCCGGCAGCGCGCNCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGc                                                                                                                                                                                                                                                                                              >  2:617621/1‑300 (MQ=255)            gcgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCGATCCGTTTAAAACGGAAAGTAACCGTACTACGGGGGTTATCAGCAGTAGTACGGTAACGCGTCCGGCAGAGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTAGTGATG‑CCGGACACGGCGGTTAGGACCCTGGCGCTATCGGC‑CCCGGGGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATGGCGTACTTTGCTCAATGACGACCCGATGTTCAAAGGCGTTTTAAcc                                                                                                                                                                                                                                                                                     <  1:11477/299‑1 (MQ=255)             cgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTATTTTGCTCCATGACGATCCGATGTTCAAAGGCGTTTTAAccc                                                                                                                                                                                                                                                                                    >  2:181213/1‑299 (MQ=255)             cgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAAccc                                                                                                                                                                                                                                                                                    >  2:678477/1‑299 (MQ=255)                            ggTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGTTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCTTGACGGGGATTACtt                                                                                                                                                                                                                                                                    <  2:278717/300‑1 (MQ=255)                            ggTTGTCGCACCGCGCGNCAGCGAACCGGCGCGCAANCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCNGTGACGGGGATTACtt                                                                                                                                                                                                                                                                    <  1:688356/300‑1 (MQ=255)                                 tCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtct                                                                                                                                                                                                                                                               <  1:127158/300‑1 (MQ=255)                                             tCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCNCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGGTTACTTTATCTCGGTGATGGGgc                                                                                                                                                                                                                                                   >  2:635549/1‑300 (MQ=255)                                             tCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGgc                                                                                                                                                                                                                                                   <  1:140616/300‑1 (MQ=255)                                                  gAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGt                                                                                                                                                                                                                                              <  1:770041/300‑1 (MQ=255)                                                       ggCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTATCTCGGTGATGGGGAGCAGTGAtgt                                                                                                                                                                                                                                         >  1:559299/1‑300 (MQ=255)                                                          gcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGCCGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGCTGTGGc                                                                                                                                                                                                                                      >  2:314729/1‑300 (MQ=255)                                                          gcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTgg                                                                                                                                                                                                                                       <  1:226810/299‑1 (MQ=255)                                                          gcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGAAGGCTAACACTGGCGATAAAATTATCATCGCTCTTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTCAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGc                                                                                                                                                                                                                                      >  2:90546/1‑300 (MQ=255)                                                            gcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTTTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCa                                                                                                                                                                                                                                     <  1:227253/299‑1 (MQ=255)                                                            gcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCNCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATTGGGCGCAGTGATGTGGCa                                                                                                                                                                                                                                     <  1:155791/299‑1 (MQ=255)                                                                                        cACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGCGCAGCGCTATGGCATGTCAGCAAAAc                                                                                                                                                                                                                         >  2:350744/1‑283 (MQ=255)                                                                                        cACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAAGCCAATTTCCCCGTGTc                                                                                                                                                                                                        >  2:205752/1‑300 (MQ=255)                                                                                        cACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAAGCAAATTTCCTAgtgt                                                                                                                                                                                                         >  2:350936/1‑299 (MQ=255)                                                                                                 tgtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGc                                                                                                                                                                                               <  1:717772/300‑1 (MQ=255)                                                                                                  gtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCTGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCt                                                                                                                                                                                              >  1:603725/1‑300 (MQ=255)                                                                                                  gtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCt                                                                                                                                                                                              <  1:4398/300‑1 (MQ=255)                                                                                                  gtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGACG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGTAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCt                                                                                                                                                                                              <  1:3175/300‑1 (MQ=255)                                                                                                    tATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGNTTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGa                                                                                                                                                                                            >  1:159882/1‑300 (MQ=255)                                                                                                        agcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CNGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                                         <  1:181213/299‑1 (MQ=255)                                                                                                         gcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGcc                                                                                                                                                                                        >  2:393434/1‑299 (MQ=255)                                                                                                         gcagTAATACGGTAAAGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCAGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGcc                                                                                                                                                                                        >  2:393542/1‑299 (MQ=255)                                                                                                          cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGc                                                                                                                                                                                      >  2:752965/1‑300 (MQ=255)                                                                                                          cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCAAGTAAG‑AAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCa                                                                                                                                                                                     >  2:557355/1‑300 (MQ=255)                                                                                                            gTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTAGGGCTCAATGCTGTGGCAAGTAAGCAAAACGCCAATTTCCTCGTGTCGATTACCGCTGGTGCCgaac                                                                                                                                                                                    >  2:101501/1‑297 (MQ=255)                                                                                                            gTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCAc                                                                                                                                                                                    >  2:532445/1‑300 (MQ=255)                                                                                                                 aCGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAAc                                                                                                                                                                               <  1:293923/300‑1 (MQ=255)                                                                                                                           gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGa                                                                                                                                                                     >  1:609340/1‑300 (MQ=255)                                                                                                                           gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATAGGGCGCCGCGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGaaaagca                                                                                                                                                                           >  2:459333/1‑289 (MQ=255)                                                                                                                           gTCCGGCAGCGCGCGCGACGGCTAACACTGACGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtctaggcggtggggtgc                                                                                                                                                                                                                                                 >  2:459139/1‑210 (MQ=255)                                                                                                                                              ggCTAACACTGGCGATAAAATTATCATCGCTATTGATT‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                  >  1:185188/1‑300 (MQ=255)                                                                                                                                              ggCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTANGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                  >  1:178147/1‑300 (MQ=255)                                                                                                                                                           gATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCg                                                                                                                                      <  1:100443/299‑1 (MQ=255)                                                                                                                                                                        tcGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCATCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTATAACCGTCGCGCCAACAGt                                                                                                                         >  2:188615/1‑299 (MQ=255)                                                                                                                                                                        tcGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑ACCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGACAATTTACTCGTGTCGATTCAAGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTAGGGGTGCTCTCTAACCGTCgcgcaaa                                                                                                                             >  2:188733/1‑292 (MQ=255)                                                                                                                                                                             aTTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAAt                                                                                                                    >  1:254645/1‑299 (MQ=255)                                                                                                                                                                             aTTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTCACCGTCGCGccccaagtgaaat                                                                                                                    >  2:105740/1‑288 (MQ=255)                                                                                                                                                                                gATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGcc                                                                                                                <  1:790926/300‑1 (MQ=255)                                                                                                                                                                                 aTG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATATCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAACCGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCAAACAGTGAAATGGCCa                                                                                                               >  2:204996/1‑300 (MQ=255)                                                                                                                                                                                                ggTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGagca                                                                                                 >  1:750319/1‑299 (MQ=255)                                                                                                                                                                                                ggTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGggca                                                                                                 >  1:279883/1‑299 (MQ=255)                                                                                                                                                                                                ggTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGagca                                                                                                 >  1:102118/1‑299 (MQ=255)                                                                                                                                                                                                ggTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCAACATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAACGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGagca                                                                                                 >  1:507904/1‑299 (MQ=255)                                                                                                                                                                                                 gTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAGATGGCCA‑GCTGGCTGGagcagc                                                                                               >  1:610957/1‑300 (MQ=255)                                                                                                                                                                                                 gTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGagcagc                                                                                               >  1:610956/1‑300 (MQ=255)                                                                                                                                                                                                    aGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCAc                                                                                             <  1:617621/299‑1 (MQ=255)                                                                                                                                                                                                     ggACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACga                                                                                           >  1:759978/1‑300 (MQ=255)                                                                                                                                                                                                       aCCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCAAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCAAACAGTGAAATGGACA‑GCCGGCTGGAGCAGCACgaga                                                                                         >  2:192144/1‑300 (MQ=255)                                                                                                                                                                                                       aCCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGAAAGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGACGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCACGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACgaga                                                                                         >  2:224649/1‑300 (MQ=255)                                                                                                                                                                                                           tGGCGCTATCTGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGaa                                                                                        <  1:393542/297‑1 (MQ=255)                                                                                                                                                                                                           tGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGaa                                                                                        <  1:393434/297‑1 (MQ=255)                                                                                                                                                                                                            ggCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTTATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACa                                                                                     >  1:640615/1‑299 (MQ=255)                                                                                                                                                                                                                 tATCGGCNCCCGGTGGTACGCTGGAGNAAAAATTGCCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCACNCTGGCTGGAGCAGCAc                                                                                             <  1:672316/290‑1 (MQ=255)                                                                                                                                                                                                                       c‑cccGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAgctgct                                                                         >  1:74582/1‑300 (MQ=255)                                                                                                                                                                                                                          ccGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAgctgct                                                                         >  1:699857/1‑298 (MQ=255)                                                                                                                                                                                                                             gtggtACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGAGCAGTGACGTGGCACGTAAGCAAAACGCAAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGTGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGCAACCGTCGGAGCTGCtgggt                                                                     >  2:812755/1‑299 (MQ=255)                                                                                                                                                                                                                              tggtACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtgg                                                                   <  1:617733/300‑1 (MQ=255)                                                                                                                                                                                                                                     cgtgagaAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGt                                                            >  1:397496/4‑300 (MQ=255)                                                                                                                                                                                                                                       ggAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGa                                                          <  1:261059/300‑1 (MQ=255)                                                                                                                                                                                                                                         agaAAAA‑‑TGTCACCATCGCCATCTCGCGTAAATTGCATACTTTGCTCAATGACGATTCAATGTTCAAACGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATTGGGCGCAGTGATGTGGCACGGAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGcgcgctagat                                                                                                                                       <  2:398681/222‑10 (MQ=255)                                                                                                                                                                                                                                                          tcgccatcgcGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAAcagtc                                         >  1:315642/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gcgTAAATTGCGTACTTNGCTCAATGACGATACGATNTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGNACAGTCAGTCTGAccc                               <  1:678980/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       tAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTTGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCATTCTGAccc                               <  1:716996/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGGTGGGGCGCAGTGGTGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTCAGCCAGGCGGt             >  2:718851/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGt             >  1:77255/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        tCAATGCCGATCCGATGTTCAAATGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTTTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                                                                       <  2:400625/177‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCt          >  1:769903/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gACGGTCCGATGTTCAAAGGCGTTTTAACCCGTGACGTGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt      <  1:90546/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gACGATCCGATGTTCAAATGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCATTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt      <  1:101501/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt      <  1:532445/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 aTCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTAc  <  1:608165/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                   GGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATG‑CCGGACACGGCGGTCAGGACCCTGGCGCTATCGGC‑CCCGGTGGTACGCGGGAGAAAAA‑‑TGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCA‑GCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTAC  >  NC_000913/4396478‑4397061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑