BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,546,601	C→T	I502I (ATC→ATT)	fimD →	type I fimbriae usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,546,601	0	C	T	100.0%	495.8 / NA	136	I502I (ATC→ATT)	fimD	type I fimbriae usher protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (72/64); total (72/64)

Only 100 of 136 total aligned reads displayed.

TCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAGCGCTTAACGTCAATATTC  >  NC_000913/4546307‑4546896

      tggctgtACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGTGCACTGTGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGaaa                                                                                                                                                                                                                                                                                                      <  2:301869/293‑1 (MQ=255)

       ggctggACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCATGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGAc                                                                                                                                                                                                                                                                                              <  1:451440/300‑1 (MQ=255)

            gACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGt                                                                                                                                                                                                                                                                                         <  1:492103/300‑1 (MQ=255)

             aCAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGtt                                                                                                                                                                                                                                                                                        >  2:35982/1‑300 (MQ=255)

                atatatGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCAAACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACTAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTAAAAAATTGAACCACAGGAAGGAGTTAtt                                                                                                                                                                                                                                                                                     >  2:573061/1‑300 (MQ=255)

                atatatGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGACATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATCACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTCA‑TTTCGCTGATACAGAAT‑ACAGTCG‑AATGAATGGCTAAAATATTGAAACACAGGACGGAGTTAtt                                                                                                                                                                                                                                                                                     >  2:440784/1‑300 (MQ=255)

                 tatatGGTGGAACGCAACTGTCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGNTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTc                                                                                                                                                                                                                                                                                    <  1:599622/300‑1 (MQ=255)

                 tatatGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGNTTTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTc                                                                                                                                                                                                                                                                                    <  1:599609/300‑1 (MQ=255)

                 tatatGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGNTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTc                                                                                                                                                                                                                                                                                    <  1:599598/300‑1 (MQ=255)

                      ggtggAACACAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGTAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTGTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGtt                                                                                                                                                                                                                                                                               <  2:460867/300‑1 (MQ=255)

                                aaCTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                                                                                                                                                                                                                                     <  1:387942/300‑1 (MQ=255)

                                          tCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACt                                                                                                                                                                                                                                                            >  2:16407/1‑299 (MQ=255)

                                          tCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTATANAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCCGGTTAAGCCGAAATTCACCGACTa                                                                                                                                                                                                                                                           >  2:591531/1‑300 (MQ=255)

                                          tCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTACCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGCTACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTa                                                                                                                                                                                                                                                           >  2:185180/1‑300 (MQ=255)

                                             ttATCGTGCTTTTAATNTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGNAGTTATTCAGGTTAAGCCNAAATTCACCGACTATTa                                                                                                                                                                                                                                                        >  1:670951/1‑300 (MQ=255)

                                              tATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                                                                                                                                                                                                                                       <  1:251353/300‑1 (MQ=255)

                                                tCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGTGCACTGGGCGCTCTGTCGGTGGATAGGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCGCTATAACAAATCGCGCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATGACaa                                                                                                                                                                                                                                                     <  1:232334/300‑1 (MQ=255)

                                                tCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                                                                                                                                                                                                                                     <  1:184531/300‑1 (MQ=255)

                                                   tGCTTTTAATTTCGTTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                                                                                                                                                                                                                                  <  2:228913/300‑1 (MQ=255)

                                                   tGCTTTTAATTTCGGTATCGTGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                                                                                                                                                                                                                                  <  2:701231/300‑1 (MQ=255)

                                                   tGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                                                                                                                                                                                                                                  <  2:701213/300‑1 (MQ=255)

                                                   tGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                                                                                                                                                                                                                                  <  1:654550/300‑1 (MQ=255)

                                                          aaTTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAGCAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                                                                                                                                                                                                                                           <  2:800152/300‑1 (MQ=255)

                                                            tttCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                                                                                                                                                                                                                                         <  1:765037/300‑1 (MQ=255)

                                                             ttCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                                                                                                                                                                                                                                        <  1:736938/300‑1 (MQ=255)

                                                                               aTGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACaa                                                                                                                                                                                                                      <  2:476119/300‑1 (MQ=255)

                                                                                tGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTCAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGAGGGAAATTACa                                                                                                                                                                                                                       >  2:17120/1‑298 (MQ=255)

                                                                                tGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCACTTATAACAAACGCGGGAAATTACAAc                                                                                                                                                                                                                     >  2:524658/1‑300 (MQ=255)

                                                                                    ggCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGNCGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc                                                                                                                                                                                                                 >  1:163301/1‑300 (MQ=255)

                                                                                          gggCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTAc                                                                                                                                                                                                           <  2:653509/300‑1 (MQ=255)

                                                                                            gcgcTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTc                                                                                                                                                                                                         <  1:2430/300‑1 (MQ=255)

                                                                                            gcgcTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAACCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTc                                                                                                                                                                                                         <  2:206030/300‑1 (MQ=255)

                                                                                                tctgtctgTGGATACGACGCATGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGc                                                                                                                                                                                                      <  2:508273/299‑1 (MQ=255)

                                                                                                  tgtctgtGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAAc                                                                                                                                                                                                   <  1:561554/300‑1 (MQ=255)

                                                                                                   gtctgtGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTTCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAACTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACt                                                                                                                                                                                                  >  1:813947/1‑300 (MQ=255)

                                                                                                   gtctgtGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACt                                                                                                                                                                                                  >  1:391935/1‑300 (MQ=255)

                                                                                                   gtctgtGGATATGACGCAGGCTAATTCCACACTTCCAGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACt                                                                                                                                                                                                  >  1:268567/1‑300 (MQ=255)

                                                                                                   gtctgtGGATATGACGCAGGCTAATTCCACACTGCCCAATGACAGTCAGCCTGACGGACCATCGGTGCGTTTTCTCTATCACAAATCGCTCAATGAATCAGGCACGAATATTTAGTTCGTGGGTTACCGTTATTCGAACAGCGGATATTTTCA‑TTTCGATGATAAAACAT‑CCAGTCG‑AATGAATGGCTACAACCTTGAAACACAGGACGGAGttctt                                                                                                                                                                                                                                                                                     >  2:674169/1‑214 (MQ=255)

                                                                                                         ggATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTAGATTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTc                                                                                                                                                                                                 >  2:223128/1‑295 (MQ=255)

                                                                                                           atatGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAACTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACCGGACGGAGTTATTCAGGTTAAGCCGCACTTCACCGACTATTACAACCTCGCTTATAACAAACGAGGGAAATTCCAACTCACCGTTACTCAGCAACTCGGgcgc                                                                                                                                                                                           >  2:748760/1‑299 (MQ=255)

                                                                                                           atatGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGca                                                                                                                                                                                          >  2:748768/1‑300 (MQ=255)

                                                                                                                  gCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAac                                                                                                                                                                                   <  1:472860/300‑1 (MQ=255)

                                                                                                                    aGGCTGATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAac                                                                                                                                                                                   <  2:716397/298‑1 (MQ=255)

                                                                                                                    aGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAacac                                                                                                                                                                                 <  1:744419/300‑1 (MQ=255)

                                                                                                                          aTTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCGGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCCACTCGGGCGCACATCAACAATGTAtt                                                                                                                                                                           >  2:30567/1‑300 (MQ=255)

                                                                                                                          aTTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAacac                                                                                                                                                                                 >  1:335473/1‑294 (MQ=255)

                                                                                                                          aTTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCACTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAacac                                                                                                                                                                                 <  2:335473/294‑1 (MQ=255)

                                                                                                                               acacTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAg                                                                                                                                                                       <  2:732443/299‑1 (MQ=255)

                                                                                                                                 acTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTAAAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTgg                                                                                                                                                                    <  1:283342/300‑1 (MQ=255)

                                                                                                                                  cTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAAAACTCACCGTTACTCAGCAACTCGGGCGCACATCACCACTGTATTTGAGTGGt                                                                                                                                                                   >  2:518867/1‑300 (MQ=255)

                                                                                                                                     cccGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGc                                                                                                                                                                >  2:558548/1‑300 (MQ=255)

                                                                                                                                         aTGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTAGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATc                                                                                                                                                            >  1:270394/1‑300 (MQ=255)

                                                                                                                                           gACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCaa                                                                                                                                                          >  1:264355/1‑300 (MQ=255)

                                                                                                                                                 cAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                                                                                                                                                                                                                                   >  1:135844/1‑205 (MQ=255)

                                                                                                                                                 cAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                                                                                                                                                                                                                                   <  2:561425/205‑1 (MQ=255)

                                                                                                                                                 cAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                                                                                                                                                                                                                                   <  2:135844/205‑1 (MQ=255)

                                                                                                                                                 cAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAcc                                                                                                                                                                                                                                                   >  1:561425/1‑205 (MQ=255)

                                                                                                                                                 cAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTAt                                                                                                                                                    >  1:50388/1‑300 (MQ=255)

                                                                                                                                                 cAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAAATCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTAt                                                                                                                                                    >  2:338837/1‑300 (MQ=255)

                                                                                                                                                   gCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTAACCGTTACTCAGCAACTAGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACGTAtt                                                                                                                                                   >  2:767683/1‑299 (MQ=255)

                                                                                                                                                         cGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTAGCTTATAACAAACGAGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAAc                                                                                                                                            >  2:47532/1‑300 (MQ=255)

                                                                                                                                                             cAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAAAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGt                                                                                                                                        >  1:96318/1‑300 (MQ=255)

                                                                                                                                                                  gttGCGTTTTCTCTATAACAAACCGCTCAATGAATCGGGCACGAATATTCAGTTAGTGGGTGACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACGACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTTGGGAACGAGTAATGt                                                                                                                                   <  2:239222/298‑1 (MQ=255)

                                                                                                                                                                  ggTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGNGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGt                                                                                                                                   <  1:181304/300‑1 (MQ=255)

                                                                                                                                                                  ggTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCNGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGt                                                                                                                                   <  2:584735/300‑1 (MQ=255)

                                                                                                                                                                  ggTGCGTTTTCTCTATAACAAATCGCGCAATGAATCAGGCACGAATATTCAGTTATTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TGTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGt                                                                                                                                   <  2:469135/300‑1 (MQ=255)

                                                                                                                                                                                ataACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACCACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCNTTACTCAGTAACTCGGGCGCACATTAACACTGTATTTGAGTGGTAGCCATCCAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTcc                                                                                                                     >  2:643404/1‑300 (MQ=255)

                                                                                                                                                                                     aaaTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACATTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCACACATCAACACTGTATTTGAGTGTTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGc                                                                                                                 <  2:184217/299‑1 (MQ=255)

                                                                                                                                                                                     aaaTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCatgc                                                                                                                 <  1:642888/299‑4 (MQ=255)

                                                                                                                                                                                     aaaTCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGc                                                                                                                 <  1:642391/299‑1 (MQ=255)

                                                                                                                                                                                             cAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAAAACTGTATTTGAGTGGTAGCCATCAAAATTATTGGGGAACGAGt                                                                                                                                        >  2:22958/1‑268 (MQ=255)

                                                                                                                                                                                              aaTGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAAt                                                                                                       <  1:35982/300‑1 (MQ=255)

                                                                                                                                                                                                tGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATAc                                                                                                     <  1:308035/300‑1 (MQ=255)

                                                                                                                                                                                                tGAATCAGGCACGAATAGTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCTTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATGGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATAc                                                                                                     <  1:440784/300‑1 (MQ=255)

                                                                                                                                                                                                             aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACNGAGTTATTCAGGTTAAGCCGAAATTCACCGACNATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGat                                                                                        >  2:623487/1‑300 (MQ=255)

                                                                                                                                                                                                                    aGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAAc                                                                                  <  1:238412/299‑1 (MQ=255)

                                                                                                                                                                                                                            ggTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACgatc                                                                        >  1:761791/1‑297 (MQ=255)

                                                                                                                                                                                                                            ggTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTc                                                                        >  1:85688/1‑300 (MQ=255)

                                                                                                                                                                                                                            ggTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAAATGG‑ACGCTc                                                                        >  2:335003/1‑300 (MQ=255)

                                                                                                                                                                                                                               tACCGTTATTCGACCATCAGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTTAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATGACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGc                                                                     <  2:348343/300‑1 (MQ=255)

                                                                                                                                                                                                                                 ccGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCta                                                                   >  1:509043/1‑300 (MQ=255)

                                                                                                                                                                                                                                    ttATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACAGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAg                                                                <  1:230110/300‑1 (MQ=255)

                                                                                                                                                                                                                                      aTTCTACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACAGTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGNAACTCGGGCGCACATCAACACTGTATTTGAGTNGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGcc                                                              <  2:613461/300‑1 (MQ=255)

                                                                                                                                                                                                                                      aTTCGACCAGCGGGTATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGcc                                                              <  1:134356/300‑1 (MQ=255)

                                                                                                                                                                                                                                      aTTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGcc                                                              <  1:451042/300‑1 (MQ=255)

                                                                                                                                                                                                                                          accagacGGATATTTTANCTTTCGCTGATACAACATNACAGTCGGCATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGGNACGCTCAGCTATAGcc                                                              <  1:674169/294‑1 (MQ=255)

                                                                                                                                                                                                                                                                     aTACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTACATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGa                                    >  2:540303/1‑296 (MQ=255)

                                                                                                                                                                                                                                                                     aTACAACAT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGAc                                   >  1:229476/1‑297 (MQ=255)

                                                                                                                                                                                                                                                                       gcaacaT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGAt                              >  1:800879/2‑300 (MQ=255)

                                                                                                                                                                                                                                                                          acaT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAACTACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCATGGCAAAAAGGACGGGATCa                            >  2:180684/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                          acaT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCa                            >  1:13372/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                          acaT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGAAGGGAtta                            >  2:404930/1‑297 (MQ=255)

                                                                                                                                                                                                                                                                          acaT‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTAGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGGGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCa                            >  2:387859/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                          acaT‑ACAGTCG‑AATGAATGGCTAAAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTAGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGAAGGGGTCa                            >  2:387848/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                             t‑ACAGTCG‑AATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATg                        <  1:17120/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                     g‑aatgaatGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCGCATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGATTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAgcgc                 <  1:748760/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                     g‑aatgaatGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAgcg                  <  1:16407/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                                           aatgGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAGCGCTTAAc            <  1:404930/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                    aacaTTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAGCGCTTAACGTCAatat    <  1:185180/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                     acaTTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAGCGCTTAACGTCAATATTc  >  2:147539/1‑300 (MQ=255)

TCCGGCTGGCTGGACAATATATGGTGGAACGCAACTGGCGGATCGTTATCGTGCTTTTAATTTCGGTATCGGGAAAAACATGGGGGCACTGGGCGCTCTGTCTGTGGATATGACGCAGGCTAATTCCACACTTCCCGATGACAGTCAGCATGACGGACAATCGGTGCGTTTTCTCTATAACAAATCGCTCAATGAATCAGGCACGAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAA‑TTTCGCTGATACAACAT‑ACAGTCG‑AATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCACCGTTACTCAGCAACTCGGGCGCACATCAACACTGTATTTGAGTGGTAGCCATCAAACTTATTGGGGAACGAGTAATGTCGATGAGCAATTCCAGGCTGGATTAAATACTGCGTTCGAAGATATCAACTGG‑ACGCTCAGCTATAGCCTGACGAAAAACGCCTGGCAAAAAGGACGGGATCAGATGTTAGCGCTTAACGTCAATATTC  >  NC_000913/4546307‑4546896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`