Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,484,291 C→T L411F (CTC→TTC)  hrpA → ATP‑dependent 3'‑>5' RNA helicase HrpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,484,2910CT82.1% 133.8 / 21.4 56L411F (CTC→TTC) hrpAATP‑dependent 3'‑>5' RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base T (22/24);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCTGGCGCGTATGGTGCT  >  NC_000913/1483992‑1484580
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                 
aCAAGCGGAACTTACGCCATACCGAAACCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCTTGGATTAAATACATTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCATGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtc                                                                                                                                                                                                                                                                                                   <  2:149502/300‑1 (MQ=255)
       gAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTc                                                                                                                                                                                                                                                                                            <  2:466878/300‑1 (MQ=255)
         aCTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCTACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTcc                                                                                                                                                                                                                                                                                           >  1:710174/1‑299 (MQ=255)
                                     ttATGCGCGGCTTTCGAACAGCGAACAAAAGAGGGTATTCCAGTCGCACAGCGGACTGCGCATTGTGCTGTCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGGGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAg                                                                                                                                                                                                                                                                <  2:497212/298‑1 (MQ=255)
                                           gcgGCTTTCGAACAGCGAACAAAAGAGGGTATTCCAGGCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGNGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGa                                                                                                                                                                                                                                                        <  2:254021/300‑1 (MQ=255)
                                                          cGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTc                                                                                                                                                                                                                                             <  1:762456/296‑1 (MQ=255)
                                                               aaaaTAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTTACCGTACCGTGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTAcc                                                                                                                                                                                                                                     <  2:763782/299‑1 (MQ=255)
                                                                       gTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTNAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGcc                                                                                                                                                                                                                            <  1:153766/300‑1 (MQ=255)
                                                                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTTGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTANATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCt                                                                                                                                                                                                                           <  1:577607/300‑1 (MQ=255)
                                                                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGTGGATTAAATACGTTATCGACCCCGTTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCATTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCCCTCGCGCCCGGAGTTTACCGAGCCGGAGATTCTGCGTACCAACCTGGCCt                                                                                                                                                                                                                           <  2:76087/300‑1 (MQ=255)
                                                                        tATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGAGACCAACTTCGCGGAAACGTCGCTGACCGTGCCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTAGTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCt                                                                                                                                                                                                                           <  2:349386/300‑1 (MQ=255)
                                                                                           aCGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCTAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGCTATTTTGCAGATGAc                                                                                                                                                                                                        <  2:462051/300‑1 (MQ=255)
                                                                                                gcATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTATCGCGCCCGGAGTTTACCGATCCGGAGATTCTGAGTACCAACCTTGCCTCGGTTATTTTGCATATGACcgc                                                                                                                                                                                                     >  2:731137/1‑298 (MQ=255)
                                                                                                     gtgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCTATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgct                                                                                                                                                                                                  >  1:415046/1‑296 (MQ=255)
                                                                                                     gtgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCAGATTTCAAAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTATCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTAGGTTATTTTGCAGATGACCGCgct                                                                                                                                                                                                  >  2:361757/1‑296 (MQ=255)
                                                                                                     gtgCTGGCGAACAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATCCGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTGCGGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCAAGGCGTCTGCCCATCAGCGTAAAGGCCGCTTTGGTCGTGTGTCAGAAGGGATCTTTCTTCGTTTCTATTCCGACGACGATTTCCTCTCGCGCACGGAGGTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTAGGTTATTTTGCAgct                                                                                                                                                                                                           >  2:742937/1‑285 (MQ=255)
                                                                                                      tgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggc                                                                                                                                                                                             <  1:213138/300‑1 (MQ=255)
                                                                                                      tgCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCAGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCgctggggc                                                                                                                                                                                             <  1:811572/300‑1 (MQ=255)
                                                                                                           gCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGc                                                                                                                                                                                        <  2:696452/300‑1 (MQ=255)
                                                                                                                                       ccGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGTGATATCGCTGCGTTCCCGTTTGTCGaa                                                                                                                                                             >  2:669995/1‑299 (MQ=255)
                                                                                                                                                               tCGACCCCGGTACAGCGCGTATCAGCCGCTGCAGCTATCGCACCAAAGTGCAGCGGTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGGACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGTCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAgg                                                                                                                                    <  1:643443/300‑1 (MQ=255)
                                                                                                                                                                 gACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGTTCGTGTGTCCTAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGAt                                                                                                                                  <  2:518613/300‑1 (MQ=255)
                                                                                                                                                                 gACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGAt                                                                                                                                  <  1:641120/300‑1 (MQ=255)
                                                                                                                                                                 gACCCCGGTACAGCGCGTATCAGCCGCGACAACTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGTTCGTGTGTCCGAATGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTTGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGAt                                                                                                                                  <  2:214941/300‑1 (MQ=255)
                                                                                                                                                                   ccccGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTAGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGAt                                                                                                                                  >  2:287501/1‑298 (MQ=255)
                                                                                                                                                                        gTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCAAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTATCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAAAGCAATATCCAGGATGgcgt                                                                                                                             >  2:414716/1‑298 (MQ=255)
                                                                                                                                                                        gTACAGCGCGTATCAGCCGCTAAAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCATCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGTGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGgcgtgc                                                                                                                           >  2:414704/1‑300 (MQ=255)
                                                                                                                                                                            aGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCATGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGTGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTc                                                                                                                        <  2:428117/299‑1 (MQ=255)
                                                                                                                                                                                         cGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACAAATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATC‑AGGATGGCGTGCGTCTGCTCGAAGAGCt                                                                                                           >  2:132645/1‑298 (MQ=255)
                                                                                                                                                                                          gCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGTCGATATCGCTGCGTTCCCGTTTTTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTc                                                                                                                   <  2:411710/290‑1 (MQ=255)
                                                                                                                                                                                          gCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTc                                                                                                                   >  1:411710/1‑290 (MQ=255)
                                                                                                                                                                                                        aCCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATcaccac                                                                                            <  1:3610/299‑1 (MQ=255)
                                                                                                                                                                                                           aaaGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCGGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACtga                                                                                         <  2:555446/299‑1 (MQ=255)
                                                                                                                                                                                                            aaGTGCAGCGTTTGCCGATTGATCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGTTCGTGTGTCCGAAGGGATCTGTANTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACtgatg                                                                                       <  2:628425/300‑1 (MQ=255)
                                                                                                                                                                                                               tGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCTAAGACGATTTCCCCTCGCGCCCGTAGTTTACCGATCCGTAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAAc                                                                                    <  2:139582/300‑1 (MQ=255)
                                                                                                                                                                                                                         gCCGATTGAGCTGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGTCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAgcgc                                                                          <  2:294491/300‑1 (MQ=255)
                                                                                                                                                                                                                         gCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAgcgc                                                                          <  2:665448/300‑1 (MQ=255)
                                                                                                                                                                                                                                 aGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAAc                                                                  <  1:198275/300‑1 (MQ=255)
                                                                                                                                                                                                                                         tCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGAcgcc                                                           >  1:513080/1‑299 (MQ=255)
                                                                                                                                                                                                                                                 gTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCAGTCg                                                  <  1:409071/300‑1 (MQ=255)
                                                                                                                                                                                                                                                     gCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTATGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCa                                               >  2:556184/1‑299 (MQ=255)
                                                                                                                                                                                                                                                             gCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCa                                      >  1:55237/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCa                                      >  2:547864/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                      ccGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGc                                 >  1:634501/1‑296 (MQ=255)
                                                                                                                                                                                                                                                                           gtgGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCTAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGAcc                        >  1:324237/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                           gtgGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGaac                        >  1:100203/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                           gtgGTCGTGTGTCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGAcc                        >  1:418979/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                 gtgtgtCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGTAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTc                  <  1:92043/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gtgtgtCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTc                  <  1:546461/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  tgtgtCTGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGTGCCCGGAGTTTACCGATCCTGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAGGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACCGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTc                  <  2:459970/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  tgtgtCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTTGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTc                  <  1:92050/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gtCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCTGgc              >  2:519503/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                     gtCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCAATGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCt                 >  2:501891/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                                      tCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGCGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGAACGCGCTGGGGATGGGTGATATCGCTGCGCTCCAGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGACGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAGCTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCTGgc              >  2:14621/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                      tCCGAAGGGATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGAACCACGTCTGgc              >  2:14499/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                              gATCTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCTGGCGCGTATGGt     >  1:510800/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                 cTGTATTCGTTTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCTGGCGCGTATGGTGCt  >  1:648590/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                 
ACAAGCTGAACTTACGCCATACCGAAATCTTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGCGGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGCCCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATGACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGCAATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAGGCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCACGTCTGGCGCGTATGGTGCT  >  NC_000913/1483992‑1484580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑