Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,637	C→A	L87L (CTC→CTA)	cbrA →	colicin M resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,637	0	C	A	100.0%	312.0 / NA	88	L87L (CTC→CTA)	cbrA	colicin M resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/52);  total (36/52)

TAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTCGCGGAGAAACATC  >  NC_000913/3869341‑3869932                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         tAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAAcg                                                                                                                                                                                                                                                                                                       <  1:52643/300‑1 (MQ=255)    tCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACTTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTTCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGt                                                                                                                                                                                                                                                                                                    <  1:773664/300‑1 (MQ=255)       cttGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAGGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGGTCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCTCAGATTTTCAGCGTCAAAACCGTCGCCGTCGCCGCATCGCTAACGCGTaa                                                                                                                                                                                                                                                                                                  >  2:238600/2‑299 (MQ=255)        ttGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACt                                                                                                                                                                                                                                                                                                <  2:656173/300‑1 (MQ=255)             aTCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCNGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCCCATCGCTAACGCGTAACTACCa                                                                                                                                                                                                                                                                                            >  1:156389/1‑299 (MQ=255)                   atgaATGTGAGTGAAAANATGGAACATTTCGACGTGNCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCNTAACTACCAGCGAAGc                                                                                                                                                                                                                                                                                     <  1:693888/300‑1 (MQ=255)                        tatgagtgaAAAGATTGAACATTTCGATGTGGCGACTATTGGCCTCGGCCCGGCATGATCGGCGTTGTCACGAAAGTTAGCCGGCAAAATGCAGTTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAANGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTTTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACACGTAACTACCAGCGAAGCgatag                                                                                                                                                                                                                                                                                <  2:246147/298‑2 (MQ=255)                         gtgagtgaAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATc                                                                                                                                                                                                                                                                               <  1:13809/300‑1 (MQ=255)                          tgagtgaAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCa                                                                                                                                                                                                                                                                              <  2:476445/300‑1 (MQ=255)                               gaAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGTCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCTAAGCTATATCAATAtt                                                                                                                                                                                                                                                                         <  2:56596/300‑1 (MQ=255)                                    gATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAANCACCAGTGTGGTACTGAAGGTTTCAGCAAACCNTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATc                                                                                                                                                                                                                                                                     >  2:625720/1‑299 (MQ=255)                                            aTTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATTGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATcaaaatgctt                                                                                                                                                                                                                                                            >  2:61172/1‑291 (MQ=255)                                                                 gCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGc                                                                                                                                                                                                                                       >  1:737016/1‑300 (MQ=255)                                                                      ggCCCGGCAGGATCGGCGTTGGCATGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTTTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCTTCAAAACCTTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGTCATGCTTTCGACTTGTGGATGAAATCGCTGATc                                                                                                                                                                                                                                  <  2:320877/300‑1 (MQ=255)                                                                      ggCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATc                                                                                                                                                                                                                                  <  1:734187/300‑1 (MQ=255)                                                                      ggCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTAATc                                                                                                                                                                                                                                  <  1:734149/300‑1 (MQ=255)                                                                               ggATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGc                                                                                                                                                                                                                         >  1:32935/1‑300 (MQ=255)                                                                                gATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCg                                                                                                                                                                                                                        <  1:650396/300‑1 (MQ=255)                                                                                           gCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTAt                                                                                                                                                                                                             >  1:348133/1‑300 (MQ=255)                                                                                           gCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCAATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGAAAGCGTTGAGATTTAt                                                                                                                                                                                                             >  2:452855/1‑300 (MQ=255)                                                                                                aaaGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGa                                                                                                                                                                                                        >  2:755299/1‑300 (MQ=255)                                                                                                     tAGCAGGCAAAATGCAGTTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGcc                                                                                                                                                                                                   <  2:8861/300‑1 (MQ=255)                                                                                                     tAGCAGGCAAAATGCAGTTGATCGCGCGGGATAAAAAGCACCAGTTTGGTACTGAAGGTTTCAGCAAATCCTGCGGCTGTCTGCTGGCACCGGACGCGCAGCGATCTTGTATTCGCGGTGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATGTTAATCGCCATGCTGTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGcc                                                                                                                                                                                                   <  2:225749/300‑1 (MQ=255)                                                                                                     tAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCTGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGcc                                                                                                                                                                                                   <  2:54005/300‑1 (MQ=255)                                                                                                          ggCAACATGCAGGTGATCGCGCTGGATAACAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATCCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACTGTCGACGTCGCCGCATCGCTAACGCGTAACTCCCAGCGCAGCTATATCAATATTAATCGCCATGCTCTCGAACTGTGGATGAAGTCGCTGCTCCCCGACAGCATTGAGGTTTAt                                                                                                                                                                                                             >  2:429324/1‑285 (MQ=255)                                                                                                           gCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGcc                                                                                                                                                                                             <  1:65871/300‑1 (MQ=255)                                                                                                                         aTCGCGCTGGATAAAAATCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCTGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCg                                                                                                                                                                               <  2:704361/299‑1 (MQ=255)                                                                                                                                 ggATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCCCGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGgatga                                                                                                                                                                      >  1:277839/1‑300 (MQ=255)                                                                                                                                 ggATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTNGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGgatga                                                                                                                                                                      >  1:574825/1‑300 (MQ=255)                                                                                                                                    tAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCACACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGTGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATaa                                                                                                                                                                   <  2:169889/300‑1 (MQ=255)                                                                                                                                    tAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATaa                                                                                                                                                                   <  2:739526/300‑1 (MQ=255)                                                                                                                                        aaGCATCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATgg                                                                                                                                                               <  1:238600/300‑1 (MQ=255)                                                                                                                                        aaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATgg                                                                                                                                                               <  1:754348/300‑1 (MQ=255)                                                                                                                                        aaGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTAGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATgg                                                                                                                                                               <  1:634171/300‑1 (MQ=255)                                                                                                                                                gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGGTCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGCAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCAt                                                                                                                                                       >  1:778420/1‑300 (MQ=255)                                                                                                                                                gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCAt                                                                                                                                                       >  1:743478/1‑300 (MQ=255)                                                                                                                                                gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCNGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCAt                                                                                                                                                       >  1:582863/1‑300 (MQ=255)                                                                                                                                                gtgtGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAACCGATGATCCCCGCAAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCAt                                                                                                                                                       >  2:340159/1‑300 (MQ=255)                                                                                                                                                  gtgGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCGCGACAGCGTTGAGGTTTATCACGACAGCGTGTGCCGAAAAATCCTGGCGTGAGGATGATAAATGGCATGTCAtt                                                                                                                                                      >  2:670865/1‑300 (MQ=255)                                                                                                                                                    ggTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCANCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCAttttt                                                                                                                                                   <  2:596692/300‑1 (MQ=255)                                                                                                                                                    ggTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCAttttt                                                                                                                                                   <  1:753406/300‑1 (MQ=255)                                                                                                                                                    ggTACTGAAGGTTTCAGCAAACCCGGCAGCGGTCCGCGGGCACCGGACGCGCAGCGATCTTTGATTCGCGAGGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTGTCAGCATCAAAACCGTCGACGGCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCACCATCGTTGAGGTTTACCACGACAGCCTGTGCCGAAAAACC‑TAAAGTGAGGATGATAAATGGCGTGTCAgtttt                                                                                                                                                   <  2:446799/300‑1 (MQ=255)                                                                                                                                                     gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATGAATGGCATGTCATTTTTc                                                                                                                                                  >  2:60857/1‑300 (MQ=255)                                                                                                                                                     gTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTc                                                                                                                                                  >  1:398764/1‑300 (MQ=255)                                                                                                                                                       aCTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGCGGTTTATCCCGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGt                                                                                                                                                >  2:337844/1‑300 (MQ=255)                                                                                                                                                         tGAAGGTTTCAGCAAATCCTTCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCGGGGCCGAAAAACC‑TGGAGTGAGgatgaggagggggatgtcagtttg                                                                                                                                                   <  2:446772/295‑20 (MQ=255)                                                                                                                                                                         cccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCaa                                                                                                                              >  1:790228/1‑300 (MQ=255)                                                                                                                                                                         cccTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCaa                                                                                                                              >  2:472162/1‑300 (MQ=255)                                                                                                                                                                               ggcggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATAtt                                                                                                                        <  1:310881/300‑1 (MQ=255)                                                                                                                                                                                gcggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGAGGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCTTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGGAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATAtt                                                                                                                        <  1:310894/299‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCTCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCNCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                      <  1:152804/299‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCTATGGGCTGGCGCTTCCTGTCGATGTGATCGCTAGTCCGCAGATTTTCAGCGTCAGAACCGTCGACGTCGCCGCATCGCGAACGAGTGACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTTGACTTGTGGATGAAATTGCTGATCCCCGCCAGCGTTGAGGTTTATCATGACAGCCTGTGCCGAAAAATC‑TTGCGTGAGGATGATGGATGGCATGTCATTTTTTGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                      <  1:721875/299‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGgat                                                                                                                                                                        <  1:760788/249‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGgat                                                                                                                                                                        >  2:760788/1‑249 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGGGCAACATATTAc                                                                                                                      <  1:464254/299‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                      <  2:656935/299‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                      <  1:510527/299‑1 (MQ=255)                                                                                                                                                                                  ggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGGGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATACTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                      <  2:277839/299‑1 (MQ=255)                                                                                                                                                                                  ggTCGGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                                                                                      <  1:510867/299‑1 (MQ=255)                                                                                                                                                                                   gTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccg                                                                                                                    <  2:781267/300‑1 (MQ=255)                                                                                                                                                                                     ctgctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgcc                                                                                                                  >  2:765440/1‑300 (MQ=255)                                                                                                                                                                                             aCCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATc                                                                                                           <  1:555620/299‑1 (MQ=255)                                                                                                                                                                                                ggACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGt                                                                                                       <  1:556908/300‑1 (MQ=255)                                                                                                                                                                                                 gACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTc                                                                                                      <  1:34484/300‑1 (MQ=255)                                                                                                                                                                                                           gATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATg                                                                                            <  1:241092/300‑1 (MQ=255)                                                                                                                                                                                                           gATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATg                                                                                            <  2:737016/300‑1 (MQ=255)                                                                                                                                                                                                                            gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGt                                                                            >  1:625225/1‑299 (MQ=255)                                                                                                                                                                                                                            gggCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATCGCGCAAACTCGATGGt                                                                            >  1:273237/1‑299 (MQ=255)                                                                                                                                                                                                                                        ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAATTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAtctc                                                               >  2:385211/1‑300 (MQ=255)                                                                                                                                                                                                                                          tGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGANAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAtctc                                                               <  2:574825/298‑1 (MQ=255)                                                                                                                                                                                                                                           gTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTAc                                                            >  1:390456/1‑300 (MQ=255)                                                                                                                                                                                                                                              gATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGTCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTAccc                                                          <  2:66319/299‑1 (MQ=255)                                                                                                                                                                                                                                              gATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTAccc                                                          <  1:289433/299‑1 (MQ=255)                                                                                                                                                                                                                                                tgtgGTCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGa                                                       >  1:442151/1‑300 (MQ=255)                                                                                                                                                                                                                                                tgtgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTTAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGa                                                       <  2:185945/300‑1 (MQ=255)                                                                                                                                                                                                                                                tgtgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGa                                                       <  1:449290/300‑1 (MQ=255)                                                                                                                                                                                                                                                     tCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTATACCCGGatcatc                                                  >  1:287655/1‑300 (MQ=255)                                                                                                                                                                                                                                                     tCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGACATGCTTTCGNCTTGTGGATGAAATCGCTGATCCCCGCCAGCGNTGAGGTTTATCCCGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTAGTGCAGACGGCTGGGAGCAACATATTCCCGCACGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTATACCCGGatcatc                                                  >  2:606240/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 aGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTaaa                                       <  1:466069/299‑1 (MQ=255)                                                                                                                                                                                                                                                                  gATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAat                                      >  2:522706/1‑299 (MQ=255)                                                                                                                                                                                                                                                                  gATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCAAGACAGCCTGTGCCGCAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGTTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACAtcta                                                               >  2:425221/1‑273 (MQ=255)                                                                                                                                                                                                                                                                    ttttCAGCTTCAAAACCGTCGACGTCGCC‑CATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGATCAACATATTAc                                                                                                                      <  2:311172/216‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCcagcag                     <  2:656524/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gTCGACGTCGCCCCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATTGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATTTCGCTATCCAGCAGTgg                  <  2:156389/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTcgc             <  1:625720/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                            tCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTAGATGGTGTGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAATGGTTcgc             >  2:28675/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                               ccgcATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTATACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTCGCGgaga        >  2:81919/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     cGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGATATCCAGCAGTGGTTCGCGGAGAAACATc  >  2:683893/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                         TAGTCTTTTGCCATCGTGATGAATGTGAGTGAAAAGATGGAACATTTCGACGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATC‑TGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGATCATCAAATCCGTAAATATGTCGCTATCCAGCAGTGGTTCGCGGAGAAACATC  >  NC_000913/3869341‑3869932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑