Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,959,589	G→A	T75T (ACC→ACT)	ppiC ←	peptidyl‑prolyl cis‑trans isomerase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,959,589	0	G	A	100.0%	216.5 / NA	64	T75T (ACC→ACT)	ppiC	peptidyl‑prolyl cis‑trans isomerase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/25);  total (39/25)

GTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAAT  >  NC_000913/3959303‑3959879                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                   gctgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTa                                                                                                                                                                                                                                                                                       >  1:792564‑M2/199‑300 (MQ=255)   tgaaaccgtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTAc                                                                                                                                                                                                                                                                                      <  1:211657‑M2/103‑1 (MQ=255)          gtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACaa                                                                                                                                                                                                                                                                               >  1:456105‑M2/190‑299 (MQ=255)          gtttatggcagagctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTACTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACaa                                                                                                                                                                                                                                                                               >  1:72540‑M2/190‑299 (MQ=255)                     agctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGAc                                                                                                                                                                                                                                                                                 <  1:698720‑M2/108‑1 (MQ=255)                     agctgcaaccgggcgacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGAc                                                                                                                                                                                                                                                                                 >  2:698720‑M2/179‑286 (MQ=255)                                   gacctgggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGcc                                                                                                                                                                                                                                                       >  2:510778‑M2/165‑298 (MQ=255)                                        gggtaaagctattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagtgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTGTTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGAACAAGAGAAAACTAATTTATCGAATGCCGGAACCa                                                                                                                                                                                                                                                >  2:300705‑M2/160‑300 (MQ=255)                                                 tattccggaaggcgcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaacngcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGAccc                                                                                                                                                                                                                                        <  2:645250‑M2/149‑1 (MQ=255)                                                              gcggtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCAcc                                                                                                                                                                                                                          >  2:486416‑M2/138‑300 (MQ=255)                                                                 gtagataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTaa                                                                                                                                                                                                                       >  1:648537‑M2/135‑300 (MQ=255)                                                                    gataactggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgagtgagcaggtaggtaagaaactgcgcggcgatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGTATAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCAAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATc                                                                                                                                                                                                                    <  2:514313‑M2/169‑1 (MQ=255)                                                                    gataacgggcaactgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATc                                                                                                                                                                                                                    >  1:423652‑M2/132‑300 (MQ=255)                                                                                ctgcgtgatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgaaagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTCCGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGACGGACCCATTTTACCCTGGCGGAATTCACCTCAATCACCGCCGCGttt                                                                                                                                                                                                        >  2:144985‑M2/120‑300 (MQ=255)                                                                                      gatgtgaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGa                                                                                                                                                                                                  >  1:247133‑M2/114‑300 (MQ=255)                                                                                           gaacgaagcgattcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCAGCGTTTGCCTGATGGGc                                                                                                                                                                                             >  2:694467‑M2/109‑300 (MQ=255)                                                                                                     attcgcagccatgcgattgagcaggtaggtaagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAgt                                                                                                                                                                                    >  1:511207‑M2/99‑299 (MQ=255)                                                                                                                                  taagaaactgcgcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTTGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAATAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCggcac                                                                                                                                                      <  2:801910‑M2/231‑3 (MQ=255)                                                                                                                                             tctgctatatgacagatatgaaacgtattgctgttgcgggttaatatcgattcagagtTCAAGGCCTTCTCCTGTAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATc                                                                                                                                           <  2:451185‑M2/242‑1 (MQ=255)                                                                                                                                             gctgctatatgacagatattaaacgtattgctgttgcgggttgatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATc                                                                                                                                           <  1:390485‑M2/242‑1 (MQ=255)                                                                                                                                             gcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGTAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGTGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATc                                                                                                                                           <  1:390268‑M2/242‑1 (MQ=255)                                                                                                                                             gcggctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATc                                                                                                                                           <  1:203790‑M2/242‑1 (MQ=255)                                                                                                                                             gcggctatatgacaaatatgaagcgtcttgctgttgcgggttaatagcgattcagagtTCAATGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGACAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATc                                                                                                                                           <  2:289852‑M2/242‑1 (MQ=255)                                                                                                                                                gctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAANTCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGc                                                                                                                                        >  1:168646‑M2/56‑300 (MQ=255)                                                                                                                                                gctatatgacagatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGc                                                                                                                                        >  1:140909‑M2/56‑300 (MQ=255)                                                                                                                                                          agatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGAt                                                                                                                              <  1:433786‑M2/255‑1 (MQ=255)                                                                                                                                                           gatatgaaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCATGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTAGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGTGTTTGTCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCTAAATCGTCACCGTTCTTAATCTGCTCGCGAAGATc                                                                                                                             >  2:381565‑M2/45‑300 (MQ=255)                                                                                                                                                            atatgaaacgtattgctgttgcgggttaatagcgattaagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATcc                                                                                                                            <  1:492414‑M2/257‑1 (MQ=255)                                                                                                                                                                 acccgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGATAAGGCCTTGCTATTAGGTACGGGAAAGCACCTTAATGATTTGGTAATCGAACTGGGTATGCAGCGTGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTAGCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGACGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGATAAGATCCagag                                                                                                                        <  2:138118‑M2/261‑1 (MQ=255)                                                                                                                                                                 aaacgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGc                                                                                                                       <  1:510778‑M2/262‑1 (MQ=255)                                                                                                                                                                   acgtattgctgttgcgggttactagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGGAGATCCAGAGCCa                                                                                                                     >  1:428711‑M2/37‑300 (MQ=255)                                                                                                                                                                   acgtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGAACCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGACATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCa                                                                                                                     >  2:194451‑M2/37‑300 (MQ=255)                                                                                                                                                                     gtattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGt                                                                                                                   >  1:735389‑M2/35‑300 (MQ=255)                                                                                                                                                                      tattgctgttgcgggttaatagcgattcagagtTCAAGGCCTTCTCCTTGATAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATTTAGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAACTTGCCGAAATCGGCACCGTTCttaacc                                                                                                                                           <  2:22701‑M2/242‑3 (MQ=255)                                                                                                                                                                                   ggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTAc                                                                                                     >  1:100374‑M2/21‑300 (MQ=255)                                                                                                                                                                                   ggttaatagcgattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTAc                                                                                                     >  2:661858‑M2/21‑300 (MQ=255)                                                                                                                                                                                      taatagcgattcagagtTCAAGGCCTCCTCCTGTATAAGGCCTGGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCTAGAAGATCCATAGCCAGTTTCTCTTCTTTTACAAg                                                                                                  <  2:38156‑M2/283‑1 (MQ=255)                                                                                                                                                                                             gattcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGc                                                                                           <  1:22762‑M2/290‑1 (MQ=255)                                                                                                                                                                                               ttcagagtTCAATGCCTTCTCCTGGATAAGGCCTTGCTATTATTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAATAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAg                                                                                         <  2:324128‑M2/292‑1 (MQ=255)                                                                                                                                                                                               ttcagagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAg                                                                                         <  1:462762‑M2/292‑1 (MQ=255)                                                                                                                                                                                                  agagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCTAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgc                                                                                      <  1:335529‑M2/295‑1 (MQ=255)                                                                                                                                                                                                  agagtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgc                                                                                      <  1:63557‑M2/295‑1 (MQ=255)                                                                                                                                                                                                     gtTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgctgc                                                                                   >  1:439869‑M2/3‑300 (MQ=255)                                                                                                                                                                                                     gtTCAAGGCCTTCTCATGGAGAAGGCATTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGCTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGtgctgc                                                                                   >  2:390424‑M2/3‑300 (MQ=255)                                                                                                                                                                                                     gtTCAAGGCCTTCTCATAGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCATGGCGGAATTCACCTACATCACCGCAGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCAAGCTTGCCGACATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTATTATTTTAAAAGGata                                                                                              >  2:391140‑M2/3‑289 (MQ=255)                                                                                                                                                                                                                 ctcCTGGAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGc                                                                       >  1:348519/1‑300 (MQ=255)                                                                                                                                                                                                                      ggAGAAGGCCTTGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGa                                                                  >  1:126657/1‑300 (MQ=255)                                                                                                                                                                                                                                ttGCTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCt                                                          <  1:19839/298‑1 (MQ=255)                                                                                                                                                                                                                                   cTATTAGTTACGGTAAAGCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGt                                                     >  1:650851/1‑300 (MQ=255)                                                                                                                                                                                                                                                    gCACCTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGCACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCATTGAGTGAATAATTGTCAggc                                      >  1:709010/1‑298 (MQ=255)                                                                                                                                                                                                                                                       ccTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGa                                 >  1:21569/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ccTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGATTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCCTGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGa                                 >  2:283053/1‑300 (MQ=255)                                                                                                                                                                                                                                                       ccTTAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCAGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGa                                 >  1:448082/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ttAATGATGTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCAAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCATGGCGGAATTCACCTAAATCACCGCCGAGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGTTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTCTCTATTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTc                                          >  2:453219/1‑289 (MQ=255)                                                                                                                                                                                                                                                            atgatgTGGTAACCGAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTa                            >  1:16029/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCTGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAg               <  1:453375/299‑1 (MQ=255)                                                                                                                                                                                                                                                                          gAACTGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGa              <  1:486416/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              tGGGTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCt          <  1:381565/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               gggTATGCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTATTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCGGGCGGCGATTTTAGCATGATCCGCAGATGCTa         >  2:81290/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ggtatgCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGACTTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCATTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTaa        >  2:802688/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gtatgCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAAc       >  1:384315/4‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gtatgCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTGTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAAc       >  1:775451/4‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gtatgCAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCAGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTCTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTACTCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATCTTAGCATGATCCACAGATGCTAAc       >  1:775379/4‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cAGCGGGCCAGTCGGCTCCAGTACCGGACAAGAGAAAACTACTTTATCGAACGCCGGAACCATCTGAACCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCACCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTATTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCAATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAAt  >  2:413355/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                   GTAAAGCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCTGGCGGAATTCACCTAAATCACCGCCGCGTTTGCCTGATGGGCAAATGGAGTGTTTCTTCGCCAGCTTGCCGAAATCGGCCCCGTTCTTAATCTGCTCGAGAAGATCCAGAGCCAGTTTCTCTTCTTTTACAAGGATATGCAGTGCTGCTGCTGTTTTTGCCATGATCGTGCCTTGAGTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAAT  >  NC_000913/3959303‑3959879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑