breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 100,946 T→C M61T (ATG→ACG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
MC JC 674,957 Δ12,894 bp IS5‑mediated ybeLgltI ybeL, ybeQ, ybeR, djlB, ybeT, ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
JC 882,572 +ATA coding (163/759 nt) deoR ← DNA‑binding transcriptional repressor DeoR
RA 915,799 G→A G83G (GGC→GGT aqpZ ← water channel AqpZ
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
JC JC 1,049,306 IS1 (+) +8 bp coding (20‑27/645 nt) gfcB ← lipoprotein GfcB
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,069,743 C→T A33T (GCA→ACA)  rutG ← pyrimidine:H(+) symporter
RA 1,098,620 T→A V245V (GTT→GTA ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,177,024 G→A D136N (GAT→AAT)  lolD → lipoprotein release complex ‑ ATP binding subunit
RA 1,184,402 G→A L15L (CTG→TTG)  potB ← spermidine preferential ABC transporter membrane subunit PotB
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,746 G→A noncoding (36/72 nt) C0293 → small regulatory RNA C0293
RA 1,205,399 G→A V410V (GTG→GTA ymfN → chimeric replication protein/phage terminase YmfN
RA 1,220,493 C→T intergenic (+1292/+1812) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,230,053 G→A D114D (GAC→GAT hlyE ← hemolysin E
RA 1,232,739 G→A P98S (CCT→TCT)  dsbB ← protein thiol:quinone oxidoreductase DsbB
RA 1,233,669 G→A S350F (TCT→TTT)  nhaB ← Na(+):H(+) antiporter NhaB
RA 1,236,874 G→A A123V (GCC→GTC)  ycgB ← PF04293 family protein YcgB
JC JC 1,237,034 IS2 (+) +5 bp coding (204‑208/1533 nt) ycgB ← PF04293 family protein YcgB
RA 1,237,645 G→A E25E (GAG→GAA dadA → D‑amino acid dehydrogenase
RA 1,248,472 G→A L215L (CTG→TTG)  dhaM ← dihydroxyacetone kinase subunit M
RA 1,255,738 G→A P72L (CCA→CTA)  ycgV ← putative autotransporter adhesin YcgV
RA 1,287,294 C→G noncoding (35/85 nt) tyrV ← tRNA‑Tyr
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,304,393 C→T T187I (ACC→ATC)  oppC → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,319,079 C→T G237D (GGT→GAT)  trpC ← fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
RA 1,319,571 C→T R73H (CGC→CAC)  trpC ← fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
RA 1,325,866 G→A R374H (CGC→CAC)  yciQ → DUF2207 domain‑containing protein YciQ
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,342,404 +TG coding (484/738 nt) pyrF → orotidine‑5'‑phosphate decarboxylase
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,373,280 C→A P20H (CCC→CAC)  ycjO → putative ABC transporter membrane subunit YcjO
RA 1,385,418 G→A E434E (GAG→GAA ycjX → DUF463 domain‑containing protein YcjX
RA 1,391,249 G→A A202V (GCC→GTC)  ycjY ← putative hydrolase YcjY
RA 1,391,804 G→A S17F (TCT→TTT)  ycjY ← putative hydrolase YcjY
RA 1,394,147 G→A Q307Q (CAG→CAA mppA → murein tripeptide ABC transporter periplasmic binding protein
RA 1,395,041 G→A T294T (ACC→ACT ynaI ← small conductance mechanosensitive channel YnaI
RA 1,405,613 G→A T291T (ACG→ACA abgR → putative LysR‑type DNA‑binding transcriptional regulator AbgR
RA 1,406,570 G→A A409V (GCG→GTG)  dgcM ← diguanylate cyclase DgcM
RA 1,409,832 G→A G108S (GGT→AGT)  dbpA → ATP‑dependent RNA helicase DbpA
RA 1,417,136 G→A S84F (TCC→TTC)  recE ← exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
RA 1,419,612 G→A intergenic (‑156/+153) ydaG ← / ← racR uncharacterized protein YdaG/DNA‑binding transcriptional repressor RacR
RA 1,445,970 G→A G31R (GGG→AGG)  ydbL → DUF1318 domain‑containing protein YdbL
RA 1,450,919 G→A S144F (TCC→TTC)  tynA ← copper‑containing amine oxidase
RA 1,461,879 G→A Q329Q (CAG→CAA paaJ → beta‑ketoadipyl‑CoA thiolase
RA 1,463,255 G→A L377L (TTG→TTA paaK → phenylacetate‑CoA ligase
RA 1,478,022 G→A Q134Q (CAG→CAA ynbA → CDP‑alcohol phosphatidyltransferase domain‑containing protein YnbA
RA 1,484,175 G→A S372N (AGC→AAC)  hrpA → ATP‑dependent 3'‑>5' RNA helicase HrpA
RA 1,491,033 T→C I44I (ATT→ATC cybB → superoxide oxidase
RA 1,538,159 T→C Y232C (TAC→TGC)  narY ← nitrate reductase Z subunit beta
JC JC 1,545,729 IS5 (+) +4 bp intergenic (‑15/+6) yddK ← / ← yddL leucine‑rich repeat domain‑containing protein YddK/putative uncharacterized protein YddL
RA 1,550,008 C→T P870S (CCG→TCG)  fdnG → formate dehydrogenase N subunit alpha
RA 1,552,547 C→T A46T (GCA→ACA)  yddM ← DNA‑binding transcriptional regulator YddM
RA 1,559,106 C→T A269T (GCA→ACA)  ddpC ← putative D,D‑dipeptide ABC transporter membrane subunit DdpC
RA 1,568,027 C→T W266* (TGG→TAG)  digH ← divisome localized glycosyl hydrolase
RA 1,570,679 C→T G456D (GGT→GAT)  gadB ← glutamate decarboxylase B
RA 1,580,035 A→G N252N (AAT→AAC ydeM ← putative anaerobic sulfatase maturation enzyme YdeM
RA 1,584,325 C→T R721H (CGC→CAC)  ydeP ← putative oxidoreductase YdeP
RA 1,586,166 C→T G107G (GGG→GGA ydeP ← putative oxidoreductase YdeP
RA 1,586,351 C→T G46S (GGC→AGC)  ydeP ← putative oxidoreductase YdeP
RA 1,596,008 A→G intergenic (‑3566/+2609) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,598,775 C→T E479K (GAA→AAA)  lsrK ← autoinducer‑2 kinase
RA 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
RA 1,601,098 C→A S48S (TCG→TCT lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,601,103 C→T V47M (GTG→ATG)  lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,609,205 C→T intergenic (‑183/+24) yneE ← / ← uxaB PF01062 family inner membrane protein YneE/tagaturonate reductase
RA 1,612,847 C→T R135R (CGG→CGA glsB ← glutaminase 2
RA 1,616,529 C→T S256F (TCT→TTT)  yneK → protein YneK
RA 1,618,305 C→T V202I (GTA→ATA)  marC ← inner membrane protein MarC
RA 1,626,582 C→T E267K (GAG→AAG)  dcp ← peptidyl‑dipeptidase Dcp
RA 1,627,018 C→T Q121Q (CAG→CAA dcp ← peptidyl‑dipeptidase Dcp
RA 1,628,822 G→A E157E (GAG→GAA rspR → DNA‑binding transcriptional repressor RspR
RA 1,634,255 C→T intergenic (+43/+55) pinQ → / ← tfaQ putative recombinase PinQ/putative tail fiber assembly protein TfaQ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,649,971 C→T P60S (CCG→TCG)  ydfD → lysis protein
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,664,769 (G)5→6 coding (264/615 nt) dmsD → redox enzyme maturation protein DmsD
RA 1,682,694 A→T Q179L (CAA→CTA)  rstA → DNA‑binding transcriptional regulator RstA
RA 1,690,549 G→A G233E (GGA→GAA)  ydgA → DUF945 domain‑containing protein YdgA
RA 1,702,757 C→T F175F (TTC→TTT add → adenosine deaminase
RA 1,739,117 C→T A228T (GCA→ACA)  punR ← DNA‑binding transcriptional activator PunR
RA 1,759,417 C→T M369I (ATG→ATA sufS ← L‑cysteine desulfurase
RA 1,774,963 C→T S93F (TCC→TTC)  aroD → 3‑dehydroquinate dehydratase
RA 1,778,585 C→T W239* (TGG→TGA ydiP ← putative DNA‑binding transcriptional regulator YdiP
RA 1,780,344 C→T A243V (GCC→GTC)  ydiQ → putative electron transfer flavoprotein subunit YdiQ
RA 1,785,863 C→T G417D (GGT→GAT)  ppsA ← phosphoenolpyruvate synthetase
RA 1,787,538 C→T P32S (CCC→TCC)  ppsR → phosphoenolpyruvate synthetase regulatory protein
RA 1,791,363 C→T G220R (GGA→AGA)  rflP ← anti‑FlhDC factor RflP
RA 1,793,377 Δ1 bp coding (182/750 nt) btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,798,265 C→T T226T (ACG→ACA pheS ← phenylalanine‑‑tRNA ligase subunit alpha
RA 1,799,455 C→T A99T (GCA→ACA)  rplT ← 50S ribosomal subunit protein L20
RA 1,806,073 A→G L4S (TTG→TCG)  ydiY ← acid‑inducible putative outer membrane protein YdiY
RA 1,815,685 C→T L607F (CTT→TTT)  katE → catalase HPII
RA 1,818,136 C→T G122D (GGT→GAT)  chbF ← monoacetylchitobiose‑6‑phosphate hydrolase
RA 1,818,821 C→T A209A (GCG→GCA chbR ← ChbR
RA 1,831,418 C→T V189I (GTC→ATC)  astC ← succinylornithine transaminase
RA 1,832,840 C→T T138I (ACC→ATC)  xthA → exodeoxyribonuclease III
RA 1,836,546 C→T R158R (CGC→CGT ynjB → putative ABC transporter periplasmic binding protein YnjB
RA 1,838,037 C→T P276S (CCT→TCT)  ynjC → putative ABC transporter membrane subunit YnjC
RA 1,874,202 G→A intergenic (‑20/‑150) yoaI ← / → yeaL protein YoaI/DUF441 domain‑containing inner membrane protein YeaL
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,911,929 2 bp→TT coding (647‑648/882 nt) htpX ← protease HtpX
RA 1,933,143 G→A H488Y (CAT→TAT)  edd ← phosphogluconate dehydratase
RA 1,953,295 G→A K198K (AAG→AAA cmoA → carboxy‑S‑adenosyl‑L‑methionine synthase
RA 1,961,288 C→T N409N (AAC→AAT argS → arginine‑‑tRNA ligase
RA 1,972,858 C→T S161N (AGC→AAC)  cheW ← chemotaxis protein CheW
MC JC 1,975,677 Δ5 bp coding (575‑579/927 nt) motB ← motility protein B
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,039,844 C→T P123S (CCA→TCA)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
MC JC 2,090,645 Δ36 bp coding (454‑489/900 nt) hisG → ATP phosphoribosyltransferase
RA 2,095,699 C→T A192V (GCC→GTC)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,099,525 A→T I30N (ATT→AAT)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,099,720 (A)5→6 intergenic (‑107/+142) ugd ← / ← gnd UDP‑glucose 6‑dehydrogenase/6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,138,991 G→A E364K (GAA→AAA)  yegH → inner membrane protein YegH
RA 2,155,880 C→T L206L (CTC→CTT mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,177,268 IS1 (+) +9 bp intergenic (‑64/+236) gatY ← / ← fbaB tagatose‑1,6‑bisphosphate aldolase 2/fructose‑bisphosphate aldolase class I
RA 2,224,169 A→G F234F (TTT→TTC pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,237,105 G→T P217T (CCG→ACG)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
JC JC 2,237,871 IS1 (–) +9 bp coding (1411‑1419/1521 nt) mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
RA 2,264,460 2 bp→AT coding (598‑599/1182 nt) setB → sugar exporter SetB
RA 2,283,534 G→A T138T (ACC→ACT yejK ← nucleoid‑associated protein YejK
RA 2,319,078 C→A D267Y (GAT→TAT)  rcsC ← histidine kinase RcsC
RA 2,360,550 Δ1 bp coding (865/1206 nt) rhmD ← L‑rhamnonate dehydratase
RA 2,392,233 C→G G270A (GGT→GCT)  nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,405,845 Δ2 bp coding (796‑797/939 nt) lrhA ← DNA‑binding transcriptional dual regulator LrhA
RA 2,423,891 C→T V207I (GTA→ATA)  hisP ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, ATP binding subunit
RA 2,443,447 G→A A562T (GCG→ACG)  mnmC → fused tRNA 5‑aminomethyl‑2‑thiouridylate methyltransferase and tRNA 5‑aminomethyl‑2‑thiouridylate synthase
RA 2,473,392 G→A intergenic (‑146/‑212) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,473,397 +T intergenic (‑151/‑207) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,492,821 G→A A145V (GCA→GTA)  frc ← formyl‑CoA transferase
RA 2,501,547 C→T L140F (CTC→TTC)  ypdC → putative AraC‑type DNA‑binding transcriptional regulator YpdC
RA 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO
RA 2,536,368 C→T intergenic (+25/+18) crr → / ← pdxK Enzyme IIA(Glc)/pyridoxine/pyridoxal/pyridoxamine kinase
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,570,589 C→T G388D (GGC→GAC)  eutE ← acetaldehyde dehydrogenase (acetylating) EutE
RA 2,578,489 C→T intergenic (‑112/‑177) maeB ← / → talA malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))/transaldolase A
RA 2,579,248 G→A V195M (GTG→ATG)  talA → transaldolase A
RA 2,591,871 G→A D89N (GAC→AAC)  dapE → succinyl‑diaminopimelate desuccinylase
RA 2,603,084 G→A V423I (GTA→ATA)  hyfB → hydrogenase 4 component B
RA 2,605,181 G→A G124D (GGC→GAC)  hyfD → hydrogenase 4 component D
RA 2,606,639 G→A P126P (CCG→CCA hyfE → hydrogenase 4 component E
RA 2,626,943 G→A L666F (CTT→TTT)  pdeF ← cyclic di‑GMP phosphodiesterase PdeF
RA 2,639,019 G→A L89L (CTG→TTG)  yfgM ← ancillary SecYEG translocon subunit
RA 2,640,757 C→T A350T (GCA→ACA)  ispG ← (E)‑4‑hydroxy‑3‑methylbut‑2‑enyl‑diphosphate synthase (flavodoxin)
RA 2,648,610 G→A G1226G (GGC→GGT yfhM ← alpha2‑macroglobulin
RA 2,653,800 C→T intergenic (‑345/‑55) timR ← / → ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
RA 2,656,852 C→T G78R (GGA→AGA)  fdx ← reduced ferredoxin
RA 2,678,538 C→T G277D (GGT→GAT)  yphF ← putative ABC transporter periplasmic binding protein YphF
RA 2,701,726 C→T A2T (GCT→ACT)  pdxJ ← pyridoxine 5'‑phosphate synthase
RA 2,716,637 G→A intergenic (‑188/‑117) grcA ← / → ung stress‑induced alternate pyruvate formate‑lyase subunit/uracil‑DNA glycosylase
RA 2,731,385 C→T intergenic (‑228/+215) rrsG ← / ← clpB 16S ribosomal RNA/chaperone protein ClpB
RA 2,732,992 G→A I394I (ATC→ATT
I394I (ATC→ATT
clpB ←
clpB ←
chaperone protein ClpB
chaperone protein ClpB
RA 2,736,071 C→T intergenic (‑60/‑75) rluD ← / → bamD 23S rRNA pseudouridine(1911/1915/1917) synthase/outer membrane protein assembly factor BamD
RA 2,744,436 A→C V32G (GTT→GGT)  rplS ← 50S ribosomal subunit protein L19
RA 2,755,976 C→T intergenic (+21/‑183) ssrA → / → intA tmRNA/integrase IntA
RA 2,756,019 C→T intergenic (+64/‑140) ssrA → / → intA tmRNA/integrase IntA
RA 2,762,148 C→T D465N (GAT→AAT)  abpB ← putative helicase YfjK
RA 2,762,272 C→T G423G (GGG→GGA abpB ← putative helicase YfjK
RA 2,764,570 C→T S195N (AGT→AAT)  abpA ← anti‑bacteriophage protein
RA 2,785,731 C→T intergenic (‑720/+31) ypjC ← / ← ileY DUF5507 domain‑containing protein YpjC/tRNA‑Ile
RA 2,789,629 C→T A215A (GCC→GCT glaH → glutarate dioxygenase GlaH
RA 2,791,031 A→G V350V (GTA→GTG lhgD → L‑2‑hydroxyglutarate dehydrogenase
RA 2,802,044 C→T P233S (CCG→TCG)  nrdE → ribonucleoside‑diphosphate reductase 2 subunit alpha
RA 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,808,797 G→A intergenic (+673/‑820) proX → / → ygaZ glycine betaine ABC transporter periplasmic binding protein ProX/L‑valine exporter, YgaZ component
RA 2,811,408 G→A intergenic (+108/‑19) mprA → / → emrA DNA‑binding transcriptional regulator MprA/multidrug efflux pump membrane fusion protein EmrA
RA 2,814,459 Δ1 bp coding (275/516 nt) luxS ← S‑ribosylhomocysteine lyase
RA 2,814,461 C→T L91L (CTG→CTA luxS ← S‑ribosylhomocysteine lyase
RA 2,816,017 C→T A141A (GCG→GCA gshA ← glutamate‑‑cysteine ligase
RA 2,831,913 C→T G126D (GGC→GAC)  norR ← DNA‑binding transcriptional dual regulator NorR
RA 2,840,190 C→T P223S (CCG→TCG)  ascF → beta‑glucoside specific PTS enzyme IIBC component
RA 2,840,328 C→T P269S (CCG→TCG)  ascF → beta‑glucoside specific PTS enzyme IIBC component
RA 2,842,650 C→T V132I (GTA→ATA)  hycI ← hydrogenase 3 maturation protease
RA 2,847,942 C→T V434I (GTC→ATC)  hycC ← formate hydrogenlyase subunit HycC
RA 2,850,416 C→T S7N (AGC→AAC)  hycA ← regulator of the transcriptional regulator FhlA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,868,958 C→T E188K (GAA→AAA)  pcm ← protein‑L‑isoaspartate O‑methyltransferase
RA 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase
RA 2,878,577 G→A P93S (CCT→TCT)  cas2 ← CRISPR‑associated endoribonuclease Cas2
RA 2,888,948 C→T K384K (AAG→AAA cysI ← sulfite reductase, hemoprotein subunit
RA 2,895,972 C→T G195E (GGA→GAA)  ygcR ← putative flavoprotein
RA 2,896,106 C→T V150V (GTG→GTA ygcR ← putative flavoprotein
RA 2,908,639 C→T G343D (GGT→GAT)  pyrG ← CTP synthetase
RA 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,929,144 C→T P306S (CCG→TCG)  sdaC → L‑serine:H(+) symporter SdaC
RA 2,930,811 C→T R412R (CGC→CGT sdaB → L‑serine deaminase II
RA 2,938,366 T→C M300T (ATG→ACG)  fucK → L‑fuculokinase
RA 2,942,432 C→T D46N (GAT→AAT)  gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,944,561 C→T A7V (GCC→GTC)  csdE → sulfur acceptor protein CsdE
RA 2,952,187 C→T S92N (AGC→AAC)  recD ← exodeoxyribonuclease V subunit RecD
RA 2,960,610 C→T A607T (GCG→ACG)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,971,926 C→T I110I (ATC→ATT tas → putative NADP(H)‑dependent aldo‑keto reductase Tas
RA 2,976,341 C→T noncoding (45/82 nt) omrB ← small regulatory RNA OmrB
RA 2,981,681 C→T D168N (GAT→AAT)  araE ← arabinose:H(+) symporter
RA 2,981,924 C→T G87R (GGG→AGG)  araE ← arabinose:H(+) symporter
RA 2,982,000 C→T Q61Q (CAG→CAA araE ← arabinose:H(+) symporter
RA 2,982,537 C→T G241D (GGT→GAT)  kduD ← putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
RA 2,983,031 C→T L76L (CTG→CTA kduD ← putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
RA 3,004,054 G→A A578V (GCT→GTT)  ygeV ← putative sigma(54)‑dependent transcriptional regulator YgeV
RA 3,040,731 G→A L31L (CTC→CTT ygfX ← protein YgfX
RA 3,040,842 2 bp→AA coding (228‑229/267 nt) sdhE ← FAD assembly factor
RA 3,043,360 G→A I88I (ATC→ATT yqfB ← N(4)‑acetylcytidine amidohydrolase
RA 3,062,875 C→T P676S (CCG→TCG)  scpA → methylmalonyl‑CoA mutase
RA 3,070,950 C→T E99K (GAA→AAA)  fbaA ← fructose‑bisphosphate aldolase class II
RA 3,071,412 C→T intergenic (‑168/+47) fbaA ← / ← pgk fructose‑bisphosphate aldolase class II/phosphoglycerate kinase
RA 3,077,625 A→G V412A (GTG→GCG)  cmtA ← mannitol‑specific PTS enzyme IICB component CmtA
RA 3,079,137 A→G M65T (ATG→ACG)  cmtB ← mannitol‑specific PTS enzyme IIA component CmtB
RA 3,089,109 A→T K276* (AAA→TAA)  galP → galactose:H(+) symporter
RA 3,095,697 C→T L200L (CTC→CTT yggS → PLP homeostasis protein
RA 3,108,763 C→T K131K (AAG→AAA speC ← constitutive ornithine decarboxylase
RA 3,112,124 C→T G309D (GGT→GAT)  yghF ← putative type II secretion system C‑type protein YghF
RA 3,118,557 C→T E186K (GAA→AAA)  yghJ ← putative lipoprotein YghJ
MC JC 3,131,341 Δ266 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,132,532 G→A P227P (CCC→CCT yghR ← putative ATP‑binding protein YghR
RA 3,156,513 C→T R387C (CGC→TGC)  yqhD → NADPH‑dependent aldehyde reductase YqhD
RA 3,161,616 G→A R352C (CGC→TGC)  ftsP ← cell division protein FtsP
RA 3,167,566 C→T V51V (GTG→GTA ygiS ← putative deoxycholate binding periplasmic protein
RA 3,174,570 C→T G276D (GGC→GAC)  parE ← DNA topoisomerase IV subunit B
RA 3,176,213 C→T E207E (GAG→GAA cpdA ← cAMP phosphodiesterase
RA 3,180,123 C→T A127V (GCC→GTC)  ygiB → DUF1190 domain‑containing protein YgiB
RA 3,188,706 C→T pseudogene (1275/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,199,080 C→T G188S (GGT→AGT)  glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
RA 3,207,283 C→T S274F (TCT→TTT)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,207,599 C→T P77S (CCG→TCG)  ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,226,845 G→A W204* (TGG→TGA ygjI → putative transporter YgjI
RA 3,256,063 G→A P197S (CCG→TCG)  cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,278,475 G→A A64V (GCC→GTC)  agaR ← DNA‑binding transcriptional repressor AgaR
RA 3,301,965 G→A E161K (GAA→AAA)  ubiU → ubiquinone biosynthesis protein UbiU
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,551,283 C→T L397L (CTG→CTA malP ← maltodextrin phosphorylase
RA 3,555,156 G→A W691* (TGG→TAG)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,690,228 G→A L12L (CTG→TTG)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,693,644 G→A F531F (TTC→TTT bcsA ← cellulose synthase catalytic subunit
RA 3,698,773 G→A G187D (GGC→GAC)  bcsG → cellulose phosphoethanolamine transferase
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,759,088 G→A A258A (GCC→GCT selB ← selenocysteyl‑tRNA‑specific translation elongation factor
RA 3,772,372 G→A G31D (GGT→GAT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,802,292 G→A P256L (CCA→CTA)  waaO ← UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase
RA 3,808,530 G→A intergenic (‑292/‑10) rirA ← / → waaA small regulatory RNA RirA/KDO transferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,822,521 G→A S41N (AGC→AAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,825,156 G→A E214E (GAG→GAA trmH → tRNA (Gm18) 2'‑O‑methyltransferase
RA 3,826,233 G→A A342T (GCA→ACA)  recG → ATP‑dependent DNA helicase RecG
RA 3,827,610 C→A T352T (ACG→ACT gltS ← glutamate:sodium symporter
RA 3,829,661 G→A M239I (ATG→ATA xanP → xanthine:H(+) symporter XanP
RA 3,832,227 T→C T771A (ACG→GCG)  yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,833,557 G→A D327D (GAC→GAT yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,845,471 G→A C503Y (TGC→TAC)  adeD → adenine deaminase
RA 3,857,116 G→A A430V (GCC→GTC)  yidJ ← putative sulfatase/phosphatase YidJ
RA 3,867,448 G→A intergenic (‑26/‑280) ibpA ← / → yidQ small heat shock protein IbpA/DUF1375 domain‑containing putative lipoprotein YidQ
RA 3,938,441 Δ1 bp coding (215/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,956,003 G→A D225N (GAT→AAT)  ilvA → threonine deaminase
RA 3,961,206 C→T A177V (GCC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,976,758 C→T L161F (CTC→TTC)  wzxE → lipid IIIECA flippase
RA 3,981,431 C→T S182L (TCG→TTG)  yifK → putative transporter YifK
RA 3,989,756 C→T V25M (GTG→ATG)  hemD ← uroporphyrinogen‑III synthase
RA 4,000,782 C→T G92D (GGC→GAC)  yigE ← DUF2233 domain‑containing protein YigE
RA 4,002,584 C→T W72* (TGG→TGA yigF ← DUF2628 domain‑containing protein YigF
RA 4,011,926 C→T S284F (TCT→TTT) 
K297K (AAG→AAA
bioP →
metR ←
biotin transporter
DNA‑binding transcriptional dual regulator MetR
RA 4,038,082 C→T noncoding (564/2905 nt) rrlA → 23S ribosomal RNA
RA 4,047,091 C→T V42V (GTC→GTT polA → DNA polymerase I
RA 4,048,733 C→T L590F (CTC→TTC)  polA → DNA polymerase I
RA 4,061,901 C→T P246L (CCG→CTG)  yihM → putative TIM barrel domain‑containing protein YihM
RA 4,079,837 C→T D29D (GAC→GAT yiiF → protein YiiF
RA 4,084,932 G→T F297L (TTC→TTA fdoG ← formate dehydrogenase O subunit alpha
RA 4,144,084 C→T S30S (AGC→AGT pflD → putative formate acetyltransferase 2
RA 4,155,646 C→T P216S (CCA→TCA)  argC → N‑acetylglutamylphosphate reductase
RA 4,171,728 C→T noncoding (92/120 nt) rrfB → 5S ribosomal RNA
RA 4,171,781 A→G intergenic (+25/‑276) rrfB → / → murB 5S ribosomal RNA/UDP‑N‑acetylenolpyruvoylglucosamine reductase
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,282,773 A→G N101N (AAT→AAC yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,297,598 G→A S590L (TCG→TTG)  fdhF ← formate dehydrogenase H
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,327,959 C→T S275S (TCC→TCT rdcA → regulator of diguanylate cyclase RdcA
RA 4,328,040 G→A Q302Q (CAG→CAA rdcA → regulator of diguanylate cyclase RdcA
RA 4,399,484 C→T A78V (GCT→GTT)  miaA → tRNA dimethylallyltransferase
RA 4,400,254 C→T intergenic (+52/‑34) miaA → / → hfq tRNA dimethylallyltransferase/RNA‑binding protein Hfq
RA 4,411,388 C→T G29G (GGC→GGT yjfK → DUF2491 domain‑containing protein YjfK
RA 4,423,337 C→T S164S (TCC→TCT ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,449,586 G→A P23S (CCG→TCG)  ppa ← inorganic pyrophosphatase
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,524,821 C→T E64K (GAA→AAA)  yjhH ← putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
RA 4,535,336 G→A intergenic (‑1283/‑1261) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423817–3424233 3424468–3424238 6–652 43 [42] [42] 43 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 270541 =NA (NA)78 (0.640) 51/598 1.2 63.4% noncoding (1/1221 nt) IS30 repeat region
?NC_000913 = 483810 45 (0.370)coding (594/3150 nt) acrB multidrug efflux pump RND permease AcrB
* ? NC_000913 270541 =NA (NA)65 (0.530) 55/598 1.0 53.7% noncoding (1/1221 nt) IS30 repeat region
?NC_000913 1666974 = 56 (0.460)coding (246/696 nt) bidA dethiobiotin synthetase BidA
* ? NC_000913 273955 =NA (NA)59 (0.480) 47/598 1.5 40.4% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 873546 87 (0.710)coding (568/2349 nt) pdeI putative c‑di‑GMP phosphodiesterase PdeI
* ? NC_000913 = 275149NA (NA)70 (0.570) 53/598 1.1 44.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 873543 = 87 (0.710)coding (565/2349 nt) pdeI putative c‑di‑GMP phosphodiesterase PdeI
* ? NC_000913 483809 =45 (0.370)79 (0.640) 56/598 1.0 63.7% coding (595/3150 nt) acrB multidrug efflux pump RND permease AcrB
?NC_000913 = 1470516 NA (NA)noncoding (1221/1221 nt) IS30 repeat region
* ? NC_000913 656630 =82 (0.670)97 (0.790) 68/598 0.5 54.2% coding (74/561 nt) pagP Lipid A palmitoyltransferase
?NC_000913 = 689045 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 65663380 (0.650)69 (0.560) 56/598 1.0 46.3% coding (77/561 nt) pagP Lipid A palmitoyltransferase
?NC_000913 687851 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 120780567 (0.550)42 (0.360) 35/566 2.3 35.2% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 91 (0.780)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)172 (1.420) 97/590 0.0 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1470516NA (NA)70 (0.570) 53/596 1.1 55.1% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 = 1666975 57 (0.470)coding (245/696 nt) bidA dethiobiotin synthetase BidA
* ? NC_000913 = 1470516NA (NA)98 (0.800) 74/598 0.3 51.0% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 = 4117985 94 (0.770)coding (106/846 nt) glpF glycerol facilitator
* ? NC_000913 = 1470516NA (NA)76 (0.620) 61/594 0.7 60.3% noncoding (1221/1221 nt) IS30 repeat region
?NC_000913 4459256 = 50 (0.410)coding (1298/1353 nt) tldE metalloprotease subunit TldE
* ? NC_000913 2288919 =NA (NA)76 (0.620) 64/598 0.6 41.8% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 2387877 106 (0.860)coding (550/717 nt) yfbN uncharacterized protein YfbN
* ? NC_000913 = 2290113NA (NA)54 (0.440) 42/598 1.9 32.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2387874 = 111 (0.910)coding (553/717 nt) yfbN uncharacterized protein YfbN
* ? NC_000913 = 394822960 (0.490)74 (0.610) 49/590 1.3 56.0% coding (144/339 nt) maoP macrodomain Ori protein
?NC_000913 3948284 = 57 (0.470)coding (199/339 nt) maoP macrodomain Ori protein
* ? NC_000913 4117984 =92 (0.760)90 (0.740) 57/594 0.9 49.5% coding (107/846 nt) glpF glycerol facilitator
?NC_000913 = 4508679 NA (NA)noncoding (1/1221 nt) IS30 repeat region
* ? NC_000913 = 445925765 (0.530)65 (0.530) 40/598 2.1 50.0% coding (1299/1353 nt) tldE metalloprotease subunit TldE
?NC_000913 = 4508679 NA (NA)noncoding (1/1221 nt) IS30 repeat region