Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,609,205	C→T	intergenic (‑183/+24)	yneE ← / ← uxaB	PF01062 family inner membrane protein YneE/tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,205	0	C	T	100.0%	209.4 / NA	57	intergenic (‑183/+24)	yneE/uxaB	PF01062 family inner membrane protein YneE/tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/25);  total (32/25)

TAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCA  >  NC_000913/1608936‑1609494                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                  tagtaAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGcac                                                                                                                                                                                                                                                                     <  2:53365/300‑1 (MQ=255) cgagatgtgtataagagacatGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGcac                                                                                                                                                                                                                                                                     <  2:300201/280‑1 (MQ=255)         gcgAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACg                                                                                                                                                                                                                                                             <  1:862966/300‑1 (MQ=255)              gATATCTTGGATAATACTGAGCCGTGCCAGANAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCANATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAg                                                                                                                                                                                                                                                        >  2:702945/1‑300 (MQ=255)                      ggATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTCCCGGTGTTATTAGCACAACGGACGTACAGCTTCgcgc                                                                                                                                                                                                                                                >  2:758024/1‑300 (MQ=255)                      ggATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGcacctgtctcttatacacatctcc                                                                                                                                                                                                                                                >  1:302375/1‑279 (MQ=255)                           ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAGTTACCTACAAGCCTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGCAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAtacc                                                                                                                                                                                                                                           >  1:93618/1‑297 (MQ=255)                           ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGTGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCAGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                                                                                                                                                                                           >  2:34588/1‑300 (MQ=255)                           ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                                                                                                                                                                                           >  1:344220/1‑300 (MQ=255)                           ataCTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCAAATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCNGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                                                                                                                                                                                           >  2:745478/1‑300 (MQ=255)                                                             ccAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGTTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                                                                                                                                                                                         <  2:173491/300‑1 (MQ=255)                                                                gtgtTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAGCTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATACCTTTTTCGAGAATCGAATCCAGGTCACTAGCAACCTGc                                                                                                                                                                                                      >  2:121177/1‑300 (MQ=255)                                                                     gttgtGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgac                                                                                                                                                                                                 >  1:976872/1‑300 (MQ=255)                                                                     gttgtGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACACGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgcc                                                                                                                                                                                                 >  2:55549/1‑298 (MQ=255)                                                                      ttgtGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgacc                                                                                                                                                                                                <  2:971930/300‑1 (MQ=255)                                                                                aCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCa                                                                                                                                                                                      <  1:931654/300‑1 (MQ=255)                                                                                aCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCa                                                                                                                                                                                      <  2:973016/300‑1 (MQ=255)                                                                                            aTCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGTTGCATGTGGCACATGTTAATGATAAAGGCACATCGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGTACGTACAGTTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTTGAACAGACCAGGAACTTGCGTCCGGt                                                                                                                                                                          >  2:418480/1‑300 (MQ=255)                                                                                            aTCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGAACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTAGAGAATTGCATCCAGGTCATTAGCACCCTGCTCGACCAAACCAGGCACTTGCGTCAgat                                                                                                                                                                          >  2:513666/1‑298 (MQ=255)                                                                                              cTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTcc                                                                                                                                                                        >  1:362581/1‑300 (MQ=255)                                                                                                   ggCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTc                                                                                                                                                                   <  1:603191/300‑1 (MQ=255)                                                                                                       tGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAANTAAGAAACCCGGTCGAAATCGGGTTCNGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAg                                                                                                                                                               <  2:686263/300‑1 (MQ=255)                                                                                                                      aTTAAACGTGGTTACGATTGTATTCTCCAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTCATGATAAAGGCATATAGGATATTGTAACTAATATGGTGAAATAGGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTCGCACAACGGGCGTACAGCTTCGCGCATCCCTCTTTCGAGAATCGCACCCAGGTCATTAGCAACCTACTCGACCAGACCAGGCACTTGCGTCAGGTCCTTCTCCCTGTGGTCTTTTTCTGcc                                                                                                                                                >  2:340739/1‑300 (MQ=255)                                                                                                                      aTTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACNTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGcc                                                                                                                                                >  1:665807/1‑300 (MQ=255)                                                                                                                               gATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATc                                                                                                                                       >  1:527295/1‑300 (MQ=255)                                                                                                                                                         aCTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTNAGAAGTAACGGTGTTATTAGCACAACNGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAACCAATCGCTAACAGTTCCTGGGTGCCGAtgac                                                                                                             >  2:672837/1‑297 (MQ=255)                                                                                                                                                           ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAacac                                                                                                           <  2:83983/300‑1 (MQ=255)                                                                                                                                                           ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAacac                                                                                                           >  1:239766/1‑300 (MQ=255)                                                                                                                                                                       tACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGACAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGNAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCNTCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCt                                                                                               <  2:787909/300‑1 (MQ=255)                                                                                                                                                                           aGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCt                                                                                           <  2:941684/300‑1 (MQ=255)                                                                                                                                                                           aGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCt                                                                                           >  1:23953/1‑300 (MQ=255)                                                                                                                                                                                  aacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGt                                                                                    <  2:588688/300‑1 (MQ=255)                                                                                                                                                                                   acaTTAGCAGGCGCTGCATGTGGCACCTGTTNATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAANGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGtt                                                                                   >  2:705544/1‑300 (MQ=255)                                                                                                                                                                                   acaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGtt                                                                                   >  1:811785/1‑300 (MQ=255)                                                                                                                                                                                   acaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCAGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACGCGGTCACGATGCTGGCTCCagagct                                                                                   >  2:395492/1‑298 (MQ=255)                                                                                                                                                                                             gcgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACg                                                                         <  2:398975/300‑1 (MQ=255)                                                                                                                                                                                              cgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCCCGATGCTGGCTCCAGAGTTGCTGATAACGt                                                                        >  2:165870/1‑300 (MQ=255)                                                                                                                                                                                                cTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTNCGCGCATCCCTTTTTCGAGAATCGCANCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTc                                                                      >  2:661150/1‑300 (MQ=255)                                                                                                                                                                                                    aTGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTccagc                                                                  >  2:635203/1‑300 (MQ=255)                                                                                                                                                                                                             cTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCa                                                         >  1:553230/1‑300 (MQ=255)                                                                                                                                                                                                               gTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATc                                                       >  1:603851/1‑300 (MQ=255)                                                                                                                                                                                                                   aTGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTcc                                                   <  2:431224/300‑1 (MQ=255)                                                                                                                                                                                                                        aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTAc                                              <  2:294143/300‑1 (MQ=255)                                                                                                                                                                                                                        aaaGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTAc                                              <  1:848886/300‑1 (MQ=255)                                                                                                                                                                                                                           ggCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCGTTAGCAAACTGCTCGACCAGACCAGGCACTTGCGNAAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCAAGCCAGTGTGCATCGTACTGTACCgg                                           >  2:235150/1‑300 (MQ=255)                                                                                                                                                                                                                           ggCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCAAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCATCCTGTACCgg                                           >  2:510255/1‑300 (MQ=255)                                                                                                                                                                                                                              atatAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGatc                                        >  2:470215/1‑299 (MQ=255)                                                                                                                                                                                                                              atatAGGATGTTGTAACTAATATGGTGAAATAAGAAACCAGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGata                                        >  2:140440/1‑300 (MQ=255)                                                                                                                                                                                                                                 taGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGt                                     <  1:419732/300‑1 (MQ=255)                                                                                                                                                                                                                                  aGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGtt                                    <  2:598432/300‑1 (MQ=255)                                                                                                                                                                                                                                   ggATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGttt                                   <  1:868661/300‑1 (MQ=255)                                                                                                                                                                                                                                        ttgtAACTAATATGGTGAAATAAGAAACCCGTTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCg                              <  1:814779/300‑1 (MQ=255)                                                                                                                                                                                                                                            aaCTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTAc                          >  1:148016/1‑300 (MQ=255)                                                                                                                                                                                                                                              cTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTAcctgtctcttatacacatctcgc                        <  1:177947/300‑22 (MQ=255)                                                                                                                                                                                                                                              cTAATATGGTGAAATAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGAATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCACAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGc                        <  1:853921/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aaTAAGAAACCCGGTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAg            <  1:45713/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ccagTCGAAATCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGcaatca  <  2:81280/297‑1 (MQ=255)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                  TAGTAAGCGCGAGGATATCTTGGATAATACTGAGCCGTGCCAGACAAAAATACGGCGCAGCCAGTGTTGTTGTGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTCCTGCTCCCAGTGGTCTTTTTCTGCCAAAACAATCGCTACCAGTTCCTGGGTGCCGATAACACGGTCACGATGCTGGCTCCAGAGTTGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCA  >  NC_000913/1608936‑1609494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑