breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,245,2340GT41.7% 13.2 / 11.0 24L25F (TTG→TTTycgYuncharacterized protein YcgY
*NC_000913766,7160GT40.0% 7.5 / 10.6 25A245S (GCG→TCG) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
*NC_0009134,075,8770AC36.1% 75.9 / 12.7 61T109P (ACC→CCC) yihXalpha‑D‑glucose‑1‑phosphate phosphatase YihX
*NC_0009134,256,0610CT33.3% 27.1 / 10.3 30E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
*NC_0009131,550,6630AC31.7% 109.7 / 11.1 82N68T (AAC→ACC) fdnHformate dehydrogenase N subunit beta
*NC_000913729,7100TG30.9% 46.6 / 12.0 42V43G (GTG→GGG) rhsCrhs element protein RhsC
*NC_0009131,445,9810TG30.9% 86.6 / 10.9 55G34G (GGT→GGGydbLDUF1318 domain‑containing protein YdbL
*NC_000913332,9910GT30.2% 75.9 / 11.9 44L207F (TTG→TTTpdeLDNA‑binding transcriptional regulator/c‑di‑GMP phosphodiesterase PdeL
*NC_000913385,7970TG29.8% 116.4 / 13.1 69V189G (GTG→GGG) tauAtaurine ABC transporter periplasmic binding protein
*NC_0009134,098,3910AC29.3% 103.9 / 10.9 78Y219S (TAC→TCC) rhaSDNA‑binding transcriptional activator RhaS
*NC_0009133,177,7480CA29.3% 38.6 / 13.8 41E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
*NC_0009133,486,3860GA28.3% 79.5 / 11.1 46A89A (GCG→GCAcrpDNA‑binding transcriptional dual regulator CRP
*NC_0009131,455,9400AC27.2% 119.6 / 13.2 85T6P (ACC→CCC) paaDphenylacetate degradation protein
*NC_0009131,876,0150AG27.0% 123.6 / 11.7 65I115V (ATT→GTT) nimT2‑nitroimidazole exporter
*NC_000913691,8830CT26.5% 88.2 / 11.0 50E153E (GAG→GAAybeYendoribonuclease YbeY
*NC_0009131,383,7220GA26.1% 87.4 / 15.9 46F80F (TTC→TTTycjWDNA‑binding transcriptional repressor YcjW
*NC_0009133,497,7280AG25.7% 160.2 / 11.7 74G242G (GGA→GGGnirCnitrite transporter NirC
*NC_0009132,296,7640TG25.4% 217.7 / 10.5 111L87F (TTA→TTCccmBcytochrome c maturation protein B
*NC_000913119,3960CT24.6% 128.1 / 14.5 69S39F (TCT→TTT) ampEprotein AmpE
*NC_0009131,260,8880CA23.2% 90.7 / 10.2 57intergenic (‑85/+40)dauA/prsaerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3052561111 (1.680)3 (0.050) 3/486 5.0 3.3% coding (958/1179 nt) ubiH 2‑octaprenyl‑6‑methoxyphenol 4‑hydroxylase
?NC_000913 = 4232178 66 (1.010)coding (1056/1350 nt) lysC aspartate kinase III
* ? NC_000913 = 97214177 (1.170)3 (0.050) 3/486 5.0 4.0% coding (390/894 nt) ycbJ putative phosphotransferase YcbJ
?NC_000913 = 2795844 69 (1.050)coding (171/663 nt) glaR DNA‑binding transcriptional repressor GlaR
* ? NC_000913 = 798949105 (1.590)3 (0.050)
+A
3/486 5.0 3.4% coding (364/996 nt) pgl 6‑phosphogluconolactonase
?NC_000913 = 4422834 68 (1.030)coding (649/651 nt) ulaD 3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD