Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,256,0610CT33.3% 27.1 / 10.3 30E136K (GAA→AAA) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (3/17);  new base T (10/0);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 9.52e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAC  >  NC_000913/4255812‑4256302
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
tattaACCAGACCCTGACGGCCAGGCAAACGTGGGCTGCCGGCGGCCAGACGTGGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCGGAGCGATAGTTTTACCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑c                                                                                                                                                                                                                                                        <  2:570946/250‑1 (MQ=255)
 attaAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTCGCCTTTTT‑ccc                                                                                                                                                                                                                                                      >  1:187169/1‑249 (MQ=255)
   taAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCGGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACg                                                                                                                                                                                                                                                     <  1:407162/250‑1 (MQ=255)
      acggacccggACGAGCAGGCAAACGGGGGCCTCCGGCGCCCATGCGTTGACGGGCAAAGGGCATCCGCGCCACGCGCCCAAGTTTCTGAGCGATAGTTTTATCCGGGCC‑GTGTCCACCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCCCTTCGCCTTTTT‑CACGcccc                                                                                                                                                                                                                                                 <  2:552507/242‑1 (MQ=255)
         gATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACGcccccgc                                                                                                                                                                                                                                              >  1:521545/1‑248 (MQ=255)
                            acgggggCCTCCGGCCGCCAGCCGGTGACGGGCAAAGTGCAAACGCGCCGCGGGCGCCAGTTGCGGAGCGATAGTTTTATCCGTGCA‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAG‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCg                                                                                                                                                                                                                         <  2:511305/247‑1 (MQ=255)
                              gTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCATCTCGCCTTTTTTCGCGCCCC‑gctggacgacaaacaaccccccc                                                                                                                                                                                                                         >  2:263845/1‑229 (MQ=255)
                                 ggCCTAAGGCAGCCAGACGTTGACGGGAAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTTACTTTTCCCTTTT‑TTCGCCCC‑cgcgcgcggcccaaaacccacccaacc                                                                                                                                                                                                                     >  1:547949/1‑232 (MQ=255)
                                  gCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCCCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑g                                                                                                                                                                                                                  <  1:306537/251‑1 (MQ=255)
                                           aGCCAGACGTGGCCGGACAAAGTGCATCCGCGCCGCGCGCGCCAGTTTTTGAGCGATATTTTTA‑CCGGGCCCGTGTCCTTCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACGGTCGCTCCCGAGCCGCAGTACAGCGAAAAAG‑TTCTGGACTCCGTGAAGCAGACGCGGCGGCGGTTTCACTTCGCCTTTTT‑CACTCCCC‑GGCGCGCGACCAAACTTC‑ACCGACACT‑GGCACCATCt                                                                                                                                                                                                         <  1:661302/251‑1 (MQ=255)
                                                                       cgcgCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TCCGCCCC‑CGCGCGCGACCAAACATCCACCG‑CACT‑GGCACCATCTTCACCACCAGAATTTGGGT‑GGT‑ACGGTg                                                                                                                                                                           >  1:530069/1‑251 (MQ=255)
                                                                             aCGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCCTTTT‑TAAGCCCC‑CGCGCGCGACCAAAAAAC‑ACCGACAAT‑GGGACCCTCTTCACATCCCGA‑CTTggggtggctacgtggcaaa                                                                                                                                                                       >  1:524468/1‑234 (MQ=255)
                                                                                      ggTTTCTGGGCTATAGTTTTACCCGTGCC‑G‑GTTCACCCCGCCATACGGCGCAGCGAAACTGACGGCGAAAAACGCACAAAACTTTGGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGTTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTc                                                                                                                                                            <  2:684802/250‑1 (MQ=255)
                                                                                      aGTTTCTGAGCGATAGTTTTATCTGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTc                                                                                                                                                            <  1:635383/251‑1 (MQ=255)
                                                                                             gAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCCCTTT‑CACGCCCC‑GGCGCGCGACCCAACACC‑ACCCACACT‑GGCACCATATGCCCATCCACA‑TTTTGGGTGCT‑ACGGTGCAAATCGAGAAAGTCGTgggac                                                                                                                                                     >  1:267239/1‑248 (MQ=255)
                                                                                                 gaaaGTTTTACCCGTGCG‑GTGTCCACCC‑GCCATACGGCGCACCGAAACGGCCGGCGAGAAACACAAAAAACTGGCGCGACCGAGCCACAGTCCAGCAAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGAAAGTCGTGGAACAGCt                                                                                                                                                 <  2:227868/248‑1 (MQ=255)
                                                                                                           tCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGGTCACTTCGCCTTTTT‑TACGCCCC‑CGCGCGCGGCCCAACATC‑ACCGCAACC‑GGCGCCCTCTGCACATCCCGA‑TTTTGGGTGCT‑ACTGGGCAAAACGAGATAGTCGTGGGACAGCTTAATATActc                                                                                                                                       >  1:213452/1‑251 (MQ=255)
                                                                                                              gtgcc‑gtgTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtcttc                                                                                                                                    <  1:134411/251‑1 (MQ=255)
                                                                                                               cgcgcgtgttcccaccgcccaccGGCGCAGCGAAACGGACGGCGAGAAACGCACAAACCTGGCTACCCCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTTACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACtctt                                                                                                                                     <  2:225497/229‑1 (MQ=255)
                                                                                                                ggcctggctccacccggcaggcggtgcAGCGAAACTGGCGGCGAGGACAGTAAAAAGCGGTCGCGGCCTAGCCACAGTCCAGC‑AAAAATATTCTATCCGCCGTTGAGCATACGCGGCGGCGGGTTCACTTCGCCTTTTG‑CTCCCCCC‑GGCGCGCGACCAAACATC‑ACCGGCACT‑GGCACCATCTGCACATCCAGA‑TTTGGGGTGCC‑ACGGTGCAAATTGAGAGAGTCGTGGATCGGCTTAATAGACtcttc                                                                                                                                    <  2:660434/226‑1 (MQ=255)
                                                                                                                   ggtatcagccc‑GCCATCGGGCCCAGCGAAACTGAGGGCGAGAAACGCAAAAACCTGTCGCACCCGGGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTc                                                                                                                                <  2:1609/242‑1 (MQ=255)
                                                                                                                    gtgtTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCTCCCTTTT‑TACGCCCC‑GGCGCGCGGCCAAACATC‑ACCCACACC‑GGCACCCTCTGCACAACCAGA‑TTTTGGTTTCTTAGCTTGCAAA‑CAGGATAGTCGGGGAACAGCTTAAAATACtctttcttcc                                                                                                                               >  1:643962/1‑245 (MQ=255)
                                                                                                                               gCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑TACGCCCC‑CGCGCGCGCCCAAACATC‑ACCGCCACC‑GGGCCCCTCTGCACAACCCCA‑TTTGGGGTGCT‑TCGGTGCACATCGAGGTAGTAGTGGAGAAGCTTAATAGaattctcctttggcgtgtgat                                                                                                                      >  2:251662/1‑230 (MQ=255)
                                                                                                                                                       ggCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTAACTTCTCCTTTTT‑TACCCCCC‑GGCGCCCGGCCAACCATC‑ACCCACCCT‑GGCCCCCCCTTCACATCCCGC‑TTTTGGGTGCT‑ACGGGGCAAATACAGATAGTCGTGGGACAGCTTTATAGACTCTTCTTTTCGCGTGTAAAAGGGGCAAACACGCGGAccgccctg                                                                                             >  2:262788/1‑248 (MQ=255)
                                                                                                                                                                                         acaGTACAGCTAAAAAT‑TTCTGTACGCCTTTAAGCATACGCATCGGCGGGTTCACTGCGCTTTTTT‑CACGCCCC‑TGCGCGCGTCCAAATAAC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGATACAAATCTGGATAGTCg                                                                                                                                                           <  2:640706/155‑1 (MQ=255)
                                                                                                                                                                                          caGTACAGCGACAAAT‑TTCTGTACGCCGTTACGCATACGCAGCGGCGGGTTCACTTTTACTTTTT‑TAATCCCC‑GGGGCGCGACCACACATC‑ACCGCCCCC‑GGGAACCTCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGGTAGTAGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAGAACACGCGGCCCGCCGTGGATGAACACAAAGCGCGGCGGGAGCGTggcgcggg                                                          >  2:442667/1‑244 (MQ=255)
                                                                                                                                                                                                               gtccGCCGTTAAGCATACGCGGCGGGGGGTTCCCTGCGCTTTTTT‑CACGCCCC‑GGCGCGCGAACAACCATC‑CCCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGAAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAAc                                         <  1:253173/245‑1 (MQ=255)
                                                                                                                                                                                                                 gccacttaaagagcccgcgagggggggTTCACTTCGCTTTTGG‑CACGCCCG‑GGCGCGCGCCAAAACACC‑CCCGACCCT‑GGCGCCATCGGCACTTCCAGT‑TTGGGGGTGCT‑GGGGTCAAAAGGGATATAGTGGGGGAACAGCTTAAGAGACTTGTTTTTGGGCGGGTAATGGGTAAACCCCCGGGCCCCGCCGTGAATGAACACAGAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTc                                    <  2:312791/227‑1 (MQ=255)
                                                                                                                                                                                                                  cGCCGTTAAGCATACGCAGCGGCGGGTTCACTTTGCCTTTTT‑TAACCCCC‑GGCGCGCGACCACACACC‑ACATACACTCGGC‑CCATCTGCCCATCCAAACTTTGGTTTCAT‑ACGGGCCAAAT‑GAGCTCGACTTGGGACAGCTTAAATGACTCTTCTTTTTGCGGGTTATAGGTGAAAACACCCGGCCCGCCCTGCATGGACACATAGAGCGGCAGTAGCGGGGCGGCGATTTTCAGCGGccataaccgggcc                                   >  1:316277/1‑239 (MQ=255)
                                                                                                                                                                                                                   gCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACCCCCC‑GGGGCGCGACCAAACCAC‑ACCCACACT‑GGCCACACCTGCACATCCAGC‑TTTGGGGTGTT‑ACGGGGCAAATAGAGATAATCGTGGAACAGCTTAATAGACTATTCTTTCGGCGGGGAAAAGGGGAACACACGCGGCCCGCCGCGAAAGAAAACAAAACGCGGCGGTAGCGTGCCGTCGATTTTCAGCGGCCCTCACggggc                                    >  1:680599/1‑247 (MQ=255)
                                                                                                                                                                                                                                 gcggcggcgGTTTCCCTTC‑CCTTTTT‑CCCCCCCCCGGCGCCCGACCAAACACC‑CCCGACACG‑GGCCCCATCTGCACATCCAGA‑TTTGGTTTGCT‑ACGGTGCAAATGGAGATAGTCGTGGAACAGCTTAATAGACTCTTTTTTCGTCGTATAATAGGTGAACACCCGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGcc                    <  1:449172/248‑1 (MQ=255)
                                                                                                                                                                                                                                                   cGCCTTTTC‑CCCGCCCC‑GGCGCGCGACAAAACATC‑CCCGACCCT‑GCCACCATCTGCACATCCAGA‑TTTGGTTTGCT‑ACGTTGCAAATCGAGATAGCCGTGGAACAGCTTAATAGACTCTTCTTCCGGCTTGTAAGAGGTGAACACACGCGTCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAc  <  1:58789/251‑1 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      
TATTAAACAGATCCTGACGAGCAGGCAAACGTGGGCCTACGGCAGCCAGACGTTGACGGGCAAAGTGCATACGCGCCACGCGCGCCAGTTTCTGAGCGATAGTTTTATCCGTGCC‑GTGTTCATCC‑GCCATACGGCGCAGCGAAACTGACGGCGAGAAACGCACAAAACTGTCGCGACCGAGCCACAGTACAGCGAAAAAT‑TTCTGTACGCCGTTAAGCATACGCAGCGGCGGGTTCACTTCGCCTTTTT‑CACGCCCC‑GGCGCGCGACCAAACATC‑ACCGACACT‑GGCACCATCTGCACATCCAGA‑TTTGGGTTGCT‑ACGGTGCAAATCGAGATAGTCGTGGAACAGCTTAATAGACTCTTCTTTCGGCGTGTAATAGGTGAACACACGCGGCCCGCCGTGAATGAACACATAGCGCGGCAGTAGCGTGCCGTCGATTTCCAGCGGCTCTAACGGGTCAGGCAAGTCATGTGCCAGACACTGGGCGCGCAAC  >  NC_000913/4255812‑4256302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑