Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913332,9910GT30.2% 75.9 / 11.9 44L207F (TTG→TTT) pdeLDNA‑binding transcriptional regulator/c‑di‑GMP phosphodiesterase PdeL
Reads supporting (aligned to +/- strand):  ref base G (1/29);  new base T (12/1);  total (14/30)
Fisher's exact test for biased strand distribution p-value = 1.07e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAA  >  NC_000913/332743‑333240
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                             
gtgATCCAACCTGTTTTCTGCTCGCAGCCTGGCGTACTGACTGGCTGTGAGGTGCTTGTCCGCTGTGAACATCCTCAAACGTGAATTATCCCACCGGCTCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATTCCGGTAAAACATTTGCTTCCGTTCAATTTCCATATTGTCATCAACGTCTCGGCGGCTTGTTGTTTGGc                                                                                                                                                                                                                                                             <  2:315354/249‑1 (MQ=255)
         ccGGTTTGCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTGATTGTCATAATGACCCGCCAACGGATGAAACAGACTGCGGATATTCGGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCAGATTGGCGTCAACGTCTCGCCGGGTTGTGTTTTTGCAGCGGGAtt                                                                                                                                                                                                                                                    <  2:345970/251‑1 (MQ=255)
               ttCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCggg                                                                                                                                                                                                                                                       <  1:596123/242‑1 (MQ=255)
               ttCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGGAGCggggggtcggg                                                                                                                                                                                                                                               >  2:596123/1‑242 (MQ=255)
                        cAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCt                                                                                                                                                                                                                                     <  2:320436/251‑1 (MQ=255)
                                            cTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGAGTTTAAAAAGAGAGCCTGAACCTGGTT‑AAAAAAtttg                                                                                                                                                                                                                >  1:655679/1‑249 (MQ=255)
                                                  ggTGCTGGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACg                                                                                                                                                                                                         <  2:479344/251‑1 (MQ=255)
                                                                        ccACAAACGGGAATTATCCCACCGGATCAGTTTATTCCGCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACAGTTGCTGCCGGACAAGTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGTTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCg                                                                                                                                                                                   <  1:400203/250‑1 (MQ=255)
                                                                              acgggccttATCCCCCCGGATCAGTTTATTCCTCTGGAGGACTCATCTGGTCTTATTGTCATCATGATCAGCCACTTGATGCAACAAACTGCGGATATTCTGCTGCCGGAAAAACATCCACTGCCCGACACTTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTAGCAGCGGGCCTTCAAAAAGCGTGTTTCAACCTGGTT‑AATAAATTAGGCAAACG‑TAAAATCAAACCG‑GTtcacactgcaat                                                                                                                                                                            >  1:464459/8‑241 (MQ=255)
                                                                              aCGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAAc                                                                                                                                                                            <  2:507184/251‑1 (MQ=255)
                                                                               cGGGAATTATCGCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCTGGGGGGTTTGAAAAAAAGGGGCTGGACCCGGGT‑TATAAAATAGG‑TAAAGGTAAAAtaaaaccggtttttgagcatacg                                                                                                                                                                            >  2:343918/1‑229 (MQ=255)
                                                                                      tATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGt                                                                                                                                                                     <  1:98457/250‑1 (MQ=255)
                                                                                       aTCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGATTTGAAAAAGAGGGTATGAACCTGGTT‑TATAAATTAGG‑GAACGATAAAATCAAGCTG‑GGTCTCGAGCTaaacggacgga                                                                                                                                                                    >  1:86170/1‑241 (MQ=255)
                                                                                                 aTCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTTCGGATAGTCTGATGCCGGTAAAACATTTGCTGCCGGTCAATTTCCATATTGGCAATAACGTCTCGGCGGGTTGTTTTTTGGCAGCGTGATTTGAAAAAGAGTGTCTGAACCt                                                                                                                                                                                                                               <  2:36314/184‑1 (MQ=255)
                                                                                                            ccTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGa                                                                                                                                              <  1:16467/251‑1 (MQ=255)
                                                                                                                    ggAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTTTAAAAAAGTGTCTGAACCTGGTT‑TATAAATTATG‑TAACGAAAAAATCCAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTTTTTCGGGAACGCCAGAAgaccg                                                                                                                                        >  1:492358/1‑245 (MQ=255)
                                                                                                                       gTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGGTTTGAAAAAGAGGGTCTGGACCTGGTT‑AATAAATTAGG‑GAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAAACCTATTTCCGTAACCCCAGAAGCCAGAGCgg                                                                                                                                   >  1:233079/1‑250 (MQ=255)
                                                                                                                         cATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGat                                                                                                                                  <  1:26852/250‑1 (MQ=255)
                                                                                                                               gTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGTAGCGGGATTTGTAAAAAAGTGTCGTAACCTCGTT‑AATAAATTTGG‑GAACGATAAAAACAAGCTGTGTT‑TCTAGCTAACGGAACTGAACCCTATTCCGCTAACGCCAAAAGCCAGAGCGAGATTTGa                                                                                                                            >  2:517170/1‑250 (MQ=255)
                                                                                                                                   tATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACa                                                                                                                          <  1:310583/248‑1 (MQ=255)
                                                                                                                                       gTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATGGGCATCAACGTCTCGGCGGGTTGTGTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTAGTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTtca                                                                                                                   <  1:440910/251‑1 (MQ=255)
                                                                                                                                         cATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTtcatc                                                                                                                 <  1:507094/251‑1 (MQ=255)
                                                                                                                                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTa                                                                                                                                                      <  2:531738/213‑1 (MQ=255)
                                                                                                                                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCCGCGGGGTTTTGAAAAGAGGGTCTTAACCTGGTT‑TATAAATTAGG‑GAAAGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGGACGTAAcccccttcccgta                                                                                                                                                      >  1:531738/1‑203 (MQ=255)
                                                                                                                                                                aaCAGGCTGAGGATATTCTGATGCCGGTAAAACATTTGCTTCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTg                                                                                         <  2:44681/251‑1 (MQ=255)
                                                                                                                                                                 aCAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTgg                                                                                        <  1:326341/251‑1 (MQ=255)
                                                                                                                                                                        gCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGAc                                                                                   <  2:86170/249‑1 (MQ=255)
                                                                                                                                                                            atatTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCCGCGGGATTTGTAAAAGAGTGTCTGAACCCGGGT‑TATAAATTTGG‑TAACGATAAAATAAAGCTG‑GTTCTCGAGCTAACGGAAAGTAACCCTATTCCGGTAACGCCCCAAGCCAGAGCGATATTTGAAAGCCTTCATCAACAC‑AACATTACCTTTGCGCTGGGTGGCttttgg                                                                             >  1:391411/1‑248 (MQ=255)
                                                                                                                                                                                   gATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCTATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGtt                                                                      <  1:343918/251‑1 (MQ=255)
                                                                                                                                                                                      gCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACTGGTTAt                                                                    <  1:219017/250‑1 (MQ=255)
                                                                                                                                                                                         ggTAAAACATTTGCTCCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTTAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑GAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAAAGTAACCCTATTCCGGTAAAGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTTCGGGTTATGCg                                                                 >  1:560759/1‑250 (MQ=255)
                                                                                                                                                                                             aaaCATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACTCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTGTGTTACGGGTTATGCTACCTa                                                            <  1:538347/251‑1 (MQ=255)
                                                                                                                                                                                                 aTTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGt                                                        <  1:193395/251‑1 (MQ=255)
                                                                                                                                                                                                 aTTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGGAGCGGGGTTTTAAAAAAAGGGTTTTAAACTGGTTTATTAAATT‑GG‑TAAAGATAAAATCAAGCTG‑GTTTTCGGGCTAAAGGAACAGAACCCTATTCCCGTAAAGCCCGAAGCCAGAGCGGTATTTGACAGCCTTCATCAGCAC‑AACATTCCCTTTTCGCTGGATGACTTTTGTAAGGGTTATGCGACCTAttgt                                                        >  1:191671/1‑248 (MQ=255)
                                                                                                                                                                                                    tgctgcCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTAc                                                                                                   <  1:114372/205‑1 (MQ=255)
                                                                                                                                                                                                     gctgcCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTTAAAAAGAGTGTCTGGACCTGGTT‑TATTAATTAGG‑GAAAGATAAAATCAAGCTG‑GTTTTCGAGCTAACGGGAAGTAACCCCATTCCCGTAACCCCCGAAGCCCGAGCGATATTTTACAGCCCTCCTCCGCACCAAC‑TTACCTTTTCCCTGGGTGACTTTGGTACGGGGTATGCGACCCATCGTTACt                                                    >  2:503822/1‑251 (MQ=255)
                                                                                                                                                                                                             aCAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAATCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCt                                                                                                 <  2:597304/198‑1 (MQ=255)
                                                                                                                                                                                                                     cATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGGAGGGGGGTTTTAAAAAGAGGGTCTGAAACCGGGG‑AATCAATTAGG‑GAAAGATAAAATAAAACTG‑GGTCTCGCGGTAAAGGGACCTAAACCCCTTTCGGGAACGCCAGAAGCCCGAGCGAAATTTTACAGGCTTCATCCGCGC‑AACAATACCTTTTAGCTGGGTGACGTTGGGACGGGGTATGCGGCCCATCGtgaatttcagggggttcccgg                                    >  1:361111/1‑231 (MQ=255)
                                                                                                                                                                                                                      atatTGGCATCATCGTCGCGGCGTGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAAGTAGG‑TAACGATAAAATCAAGCTG‑GTTCGCGAGCTAACGGAACGGAACCCTATTCCTGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGTATGACTTTGGTACGGGTTATGCGACCTAGCGTTACTTGCAGGCGTTCCCGGTc                                   <  2:665159/251‑1 (MQ=255)
                                                                                                                                                                                                                                cAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTTCCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTttatt                          <  1:302004/250‑1 (MQ=255)
                                                                                                                                                                                                                                  aCGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAg                       <  1:503822/251‑1 (MQ=255)
                                                                                                                                                                                                                                    gTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCa                                                <  1:376731/224‑1 (MQ=255)
                                                                                                                                                                                                                                             ggTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAAGCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTc            <  2:492358/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     tttGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTtgtg     <  2:191671/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        ggCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCACGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCaa  >  1:114966/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        ggCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGGTCGATAAGTCATTTGTGCaa  >  1:1945/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                             
TGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTACTGACGGGCTGTGAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATTCCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTT‑AATAAATTAGG‑TAACGATAAAATCAAGCTG‑GTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCAC‑AACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAA  >  NC_000913/332743‑333240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑