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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 155,463 | A→T | *247K (TAA→AAA) | yadV ← | putative fimbrial chaperone YadV |
RA | 557,635 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
MC JC | 575,786 | Δ991 bp | IS5‑mediated | nmpC ← | putative outer membrane porin NmpC |
JC JC | 577,688 | IS3 (+) +3 bp | coding (76‑78/498 nt) | rrrD → | lysozyme |
RA | 1,048,596 | G→A | G32G (GGC→GGT) | gfcC ← | capsule biosynthesis GfcC family protein |
MC JC | 1,048,805 | Δ48,085 bp | [gfcB]–ycdU | 45 genes [gfcB], gfcA, insA4, insB4, cspH, cspG, ymcF, ymcE, gnsA, yccM, torS, torT, torR, torC, torA, torD, cbpM, cbpA, yccE, agp, yccJ, wrbA, ymdF, rutG, rutF, rutE, rutD, rutC, rutB, rutA, rutR, putA, ymdG, putP, efeO, efeB, phoH, pgaD, pgaC, pgaB, pgaA, dgcT, insF4, insE4, ycdU |
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JC JC | 1,105,376 | IS2 (–) +5 bp | intergenic (+83/‑34) | csgC → / → ymdA | curlin chaperone/uncharacterized protein YmdA |
MC JC | 1,299,499 | Δ1,199 bp | insH21 | insH21 | |
RA | 1,301,917 | C→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
RA | 1,301,919 | G→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
JC JC | 2,016,437 | Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp | coding (570‑577/687 nt) | fliH → | flagellar biosynthesis protein FliH |
RA | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
RA | 2,867,454 | 2 bp→AA | coding (97‑98/993 nt) | rpoS ← | RNA polymerase sigma factor RpoS |
RA | 2,926,772 | C→A | G155G (GGC→GGA) | ppnN → | nucleotide 5'‑monophosphate nucleosidase |
MC JC | 3,110,591 | Δ24,234 bp | [yghD]–yghT | 20 genes [yghD], yghE, yqhJ, yghF, yghG, pppA, yghJ, glcA, glcB, glcG, glcF, glcE, glcD, glcC, yghO, insH9, yghQ, yghR, yghS, yghT |
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RA | 3,268,835 | G→T | V141L (GTA→TTA) | yhaC → | uncharacterized protein YhaC |
RA | 3,486,205 | C→A | T29K (ACG→AAG) | crp → | DNA‑binding transcriptional dual regulator CRP |
RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
JC JC | 3,707,484 | IS3 (+) +3 bp | coding (220‑222/1608 nt) | dppA ← | dipeptide ABC transporter periplasmic binding protein |
JC JC | 3,720,136 | IS3 (–) +3 bp :: +TCA | coding (88‑90/213 nt) | cspA → | cold shock protein CspA |
JC JC | 3,890,411 | IS1 (–) +9 bp | coding (176‑184/1248 nt) | tnaB → | tryptophan:H(+) symporter TnaB |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
RA | 4,558,907 | T→A | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1978495 | 1979270–1978503 | 9–776 | 50 [0] | [1] 36 | insB5–insA5 | insB5,insA5 |
* | * | ÷ | NC_000913 | 2406788 | 2407179 | 392 | 75 [0] | [0] 59 | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
* | * | ÷ | NC_000913 | 3423828–3424438 | 3424438 | 1–611 | 10 [9] | [9] 10 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 257907 | 0 (0.000) | 32 (0.500) | 31/472 | 0.9 | 100% | intergenic (+8/+16) | crl/insB9 | RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B |
? | NC_000913 | 258684 = | 0 (0.000) | pseudogene (9/331 nt) | crl | RNA polymerase holoenzyme assembly factor Crl | |||||
* | ? | NC_000913 | 1207790 = | 16 (0.240) | 40 (0.650) | 36/456 | 0.7 | 74.8% | coding (290/630 nt) | ycfK | protein StfP |
? | NC_000913 | 1209619 = | 12 (0.190) | intergenic (‑500/‑66) | tfaE/pinE | putative tail fiber assembly protein TfaE/site‑specific DNA recombinase | |||||
* | ? | NC_000913 | = 1207805 | 15 (0.230) | 39 (0.630) | 38/456 | 0.6 | 75.0% | coding (305/630 nt) | ycfK | protein StfP |
? | NC_000913 | = 1209602 | 12 (0.190) | intergenic (‑483/‑83) | tfaE/pinE | putative tail fiber assembly protein TfaE/site‑specific DNA recombinase | |||||
* | ? | NC_000913 | = 1397238 | NA (NA) | 50 (0.760) | 48/488 | 0.4 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 1978494 | 0 (0.000) | intergenic (‑297/+24) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB | |||||
* | ? | NC_000913 | 1978503 = | NA (NA) | 75 (1.140) | 65/486 | 0.1 | 100% | noncoding (768/768 nt) | IS1 | repeat region |
? | NC_000913 | = 2406787 | 0 (0.000) | intergenic (‑146/‑774) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA | |||||
* | ? | NC_000913 | 1979271 = | 1 (0.020) | 35 (0.530) | 34/488 | 0.9 | 97.2% | intergenic (‑56/‑482) | insA5/uspC | IS1 family protein InsA/universal stress protein C |
? | NC_000913 | = 2102947 | NA (NA) | pseudogene (443/446 nt) | wbbL | interrupted rhamnosyltransferase WbbL | |||||
* | ? | NC_000913 | 2407180 = | 0 (0.000) | 59 (0.900) | 56/486 | 0.2 | 100% | intergenic (‑539/‑381) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
? | NC_000913 | 3583428 = | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region |