breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 155,463 A→T *247K (TAA→AAA)  yadV ← putative fimbrial chaperone YadV
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
MC JC 575,786 Δ991 bp IS5‑mediated nmpC ← putative outer membrane porin NmpC
JC JC 577,688 IS3 (+) +3 bp coding (76‑78/498 nt) rrrD → lysozyme
RA 1,048,596 G→A G32G (GGC→GGT gfcC ← capsule biosynthesis GfcC family protein
MC JC 1,048,805 Δ48,085 bp [gfcB]ycdU 45 genes
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,917 C→T Q246Y (CAG→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,301,919 G→T Q246Y (CAG→TAT oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 2,016,437 Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp coding (570‑577/687 nt) fliH → flagellar biosynthesis protein FliH
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,867,454 2 bp→AA coding (97‑98/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
MC JC 3,110,591 Δ24,234 bp [yghD]yghT 20 genes
RA 3,268,835 G→T V141L (GTA→TTA)  yhaC → uncharacterized protein YhaC
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,707,484 IS3 (+) +3 bp coding (220‑222/1608 nt) dppA ← dipeptide ABC transporter periplasmic binding protein
JC JC 3,720,136 IS3 (–) +3 bp :: +TCA coding (88‑90/213 nt) cspA → cold shock protein CspA
JC JC 3,890,411 IS1 (–) +9 bp coding (176‑184/1248 nt) tnaB → tryptophan:H(+) symporter TnaB
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,558,907 T→A K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978495 1979270–1978503 9–776 50 [0] [1] 36 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2406788 2407179 392 75 [0] [0] 59 lrhA/alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
* * ÷ NC_000913 3423828–3424438 3424438 1–611 10 [9] [9] 10 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)32 (0.500) 31/472 0.9 100% intergenic (+8/+16) crl/insB9 RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 1207790 =16 (0.240)40 (0.650) 36/456 0.7 74.8% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 12 (0.190)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780515 (0.230)39 (0.630) 38/456 0.6 75.0% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 12 (0.190)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 1397238NA (NA)50 (0.760) 48/488 0.4 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1978503 =NA (NA)75 (1.140) 65/486 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 2406787 0 (0.000)intergenic (‑146/‑774) lrhA/alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
* ? NC_000913 1979271 =1 (0.020)35 (0.530) 34/488 0.9 97.2% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2407180 =0 (0.000)59 (0.900) 56/486 0.2 100% intergenic (‑539/‑381) lrhA/alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region