Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,888	0	C	A	23.2%	90.7 / 10.2	57	intergenic (‑85/+40)	dauA/prs	aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand):  ref base C (35/8);  new base A (0/13);  total (35/22)
Fisher's exact test for biased strand distribution p-value = 1.08e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAGAGAAGATCGGGTGAGTCGCGTA  >  NC_000913/1260640‑1261137                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          ccacTACCAATAGCCAACGCCATCGCCAGCGGGAGAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACaa                                                                                                                                                                                                                                                         <  2:369817/251‑1 (MQ=255)   acTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATCTTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACaaa                                                                                                                                                                                                                                                        >  1:682702/1‑250 (MQ=255)      aCCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACaaaaa                                                                                                                                                                                                                                                      <  1:455369/249‑1 (MQ=255)         aaTAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTGCCAGCAAGCGTCGAGCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGG‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGG‑CA‑TACAAATGGATAG‑TACA‑TGCTAAAAAAccc                                                                                                                                                                                                                                                 <  2:36318/251‑1 (MQ=255)          aTAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACccg                                                                                                                                                                                                                                                >  1:612736/1‑251 (MQ=255)                 aCGCCATCGCCAGCGGGATAGCAATAATCCAGACGGTTATCCCGGCCATCCGGTCACGGGTAAACCGTGCGGCCGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGACAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGA‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGc                                                                                                                                                                                                                                         >  2:631118/1‑251 (MQ=255)                      aTCGCCAGCGGGATAGCAATAATCCCGACGGTTCTCCCGGCACTCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCACGCGCCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTCCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGATGG‑CGGCATAGGT‑CA‑TACAAATGGAAAT‑TACA‑GACAAAACACACCGCCGCAGCGGGTc                                                                                                                                                                                                                                    >  2:386626/1‑251 (MQ=255)                                 gATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCggg                                                                                                                                                                                                                         >  1:461485/1‑251 (MQ=255)                                   aaGCAATAATCCCGACGTTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGAAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATTTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGAATAGGT‑CA‑TAAAAATGGATAT‑TACG‑GAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGtt                                                                                                                                                                                                                       <  1:467041/250‑1 (MQ=255)                                       aataatCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGAATATTTTTCTTTCCAGCAAGAGCCGATCAGAGCGCGGAAAGCCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAATTAAATGGCAGGTGAAGA‑GGGCAAAGGT‑CA‑TAAAAAGGGATAT‑TACG‑GACAAAAAAACCGGCGGCACCGGGTCTTTGAGCCGGGTTCgct                                                                                                                                                                                                                   >  1:240461/1‑249 (MQ=255)                                        ataatCCCGACGGATATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                                                                                                                                                                  >  1:577043/1‑251 (MQ=255)                                                                   agtcaaCGGGTAAACCTGGCGGCAGTATTTTTGTCTTTCAAGCGAGCGTGTGCCAGGGCAGAGAAAGGCTTCAAATGGGAGAAAAA‑TTTTTTGTTCACAATAATGTTTCTGCCGGGGGCGCATCGTGTTT‑CAACAAAATGGCGGGGGAGGG‑AGGCAGAGGT‑CA‑TAAAATTGGATTT‑TACA‑AAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                                                                                                                                       <  2:521789/246‑1 (MQ=255)                                                                   aGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                                                                                                                                       >  2:655569/1‑251 (MQ=255)                                                                       caggggTAATCCGTGCGGCATAATATTTGTCTTCCCGGCAAGCGTCGTGCAGGGCGCGGAAGGGCATAACATGTGAGAAAAA‑TTTTTTGTAAACAAAAATTTTTACTCCGTGAGCGCATCGTCTGT‑GAGCTAAGTGGGAGGGAAATG‑AGGCAT‑GGG‑CAGTAAAAGTGGATAT‑TACA‑GATAAAAATTGCCGCCGCAGCGGGTCTTTGAGCCGGTTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt                                                                                                                                                                                    <  1:691316/247‑1 (MQ=255)                                                                               aaCCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAgtgt                                                                                                                                                                                                             >  1:490408/1‑217 (MQ=255)                                                                                  cGTGCGGCCGTCTATTTTTCTTTACAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCCTAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCAGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                                                                                                                                        >  2:432825/1‑251 (MQ=255)                                                                                          aGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTc                                                                                                                                                                                                                                    >  1:155691/1‑183 (MQ=255)                                                                                           gtaatgttttcgccccgtgaggCGTCGATCAGCGCGCTAAAAGGCATCACAGGT‑AGGAAAAATTTTTTGTACAAAATAATGTTTAATCCGTGGGTGCAAGCTATGT‑AAAAAAAAGTGCAGGAGAAGG‑CGACATAGGA‑AA‑TACAAATGGA‑ATGTACA‑GAAAAAAAAGCCCGCCGCAGCGGGTCTGTGAGCCGGGTTCGAGTAGTGTTCGAACATGGCAGAGATCGATTCTTCGGTGCTTATACGGCGAATCg                                                                                                                                                               <  2:335952/230‑1 (MQ=255)                                                                                               ttttttCTTCCCCGCAAGGGTCGAACAGAGCGCGGAAGGGCATCACATGGGAGAAAAA‑TTTTTTGTCCAAAAAAATTTTTCATCCGGGGGCGCAACAGCTGT‑CAAATAAATGGCAGGTAAAGG‑AGGCATGGGT‑AA‑TAAAAAGGTATAT‑TAAA‑GAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTGCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt                                                                                                                                                            <  2:597024/249‑1 (MQ=255)                                                                                                    tcttccCAGCAACCGGCGATCAGAGCGCGAAAAGGCAGCTCTTGTGAGGAAAT‑TTTTTTTTTCATAATATTGTTTCCCCCGTGGGGGATTCACCTTC‑CAACTAAATGGCGGTTGAAGG‑AGGAAT‑GGTGCA‑TGCAAGGGGAGAT‑GCCA‑GCCAAAAAAACCCGCCGCAGCGGGGCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCTGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGcc                                                                                                                                                       <  1:633930/250‑1 (MQ=255)                                                                                                       ttcCAGCAAGCGTCGATCAGAGCGCGGAAAGGAATCACATGTGAGAAAAA‑TATTTTGTTCACAATAATGTTTCACCCGTGAGCGCATCATCTGT‑AAACAAAAGGGCAGGGAAAGG‑AGGCTTAGGT‑CA‑TAAAAAGGGATAT‑TACA‑GACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAg                                                                                                                                                     <  1:15691/249‑1 (MQ=255)                                                                                                       taccAGCAAGCGTCGATCAGAGCGCGAAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGc                                                                                                                                                    <  1:591531/248‑1 (MQ=255)                                                                                                       ccccAGCAAGCGGCGATCAGAGCGCGAAAAGGCACAAAATGGGAGAAAAA‑TTTTTTGTTCACAATAATGTTTCATCGGGGAGCGCATCATCTGT‑CAACAAAAGGGAAGGTGAAGG‑GGGCATAGGT‑AA‑AAAAAATGAATAT‑‑ACAAGAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCa                                                                                                                                                   <  2:329200/249‑1 (MQ=255)                                                                                                              aaGCGTCGATCAGAGCGCGGAAAGGCAGCACATGTGAGAAAAA‑TATTTTGTTCAAAATAATGTTTCATCGGTGAGCGCATCATCTGT‑AAAATAAATGGCAGGTGAAGG‑GGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa                                                                                                                                            <  2:127471/251‑1 (MQ=255)                                                                                                               aGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTT‑GATTAGTGTTCGAACATGGCag                                                                                                                                                                                                >  1:547800/1‑197 (MQ=255)                                                                                                               aGCGTCGATCAGAGCGCGGAAAGGCATCACAGGTGAGAAAAA‑TATTTTGTTCAAAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACAAAAGGGCAGGTGAAGG‑AGGCATAGGT‑CT‑TAAAAATGAATAT‑TACA‑GAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTT‑GATTAGTGTTCGAACATGGCag                                                                                                                                                                                                <  2:547800/197‑1 (MQ=255)                                                                                                                  gTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACACGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGCATTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGCCCAGCATACCTGACAgg                                                                                                                                        >  1:414757/1‑251 (MQ=255)                                                                                                                                ggAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGAAACGACGAATCGCTTCGGCCAGCCTACCTTACAGGGTCAGAGTAAGCAc                                                                                                                          >  2:391218/1‑251 (MQ=255)                                                                                                                                         tCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGc                                                                                                                   >  1:566058/1‑249 (MQ=255)                                                                                                                                          cacaTGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGGGAGCGCATCATCTGT‑CAACTAAATGGAAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAgg                                                                                                                >  1:30521/1‑250 (MQ=255)                                                                                                                                          cacaTGTGAGAAAAA‑TTTTTTGTTCACAATAATTTTGCACCCGGGATCTCATCATCTGT‑AAACAAAGTGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGAATAT‑TACA‑GCAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTGGCTGATACGACGAATCGCTTTGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAg                                                                                                                 <  1:101015/250‑1 (MQ=255)                                                                                                                                            caTGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGtgg                                                                                                              >  1:308933/1‑250 (MQ=255)                                                                                                                                                gtgaggacaccatTTTGTTCACAATAATGTTTCATCCGTGAGAGCATCATCTGC‑CAACTAAATAGCAGGTGAAGG‑AGGCATAGGT‑CA‑TCCAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGCTACGACGCATCGCTTGGGCCAGAATACCTGTCCGGGTCAGAGTACGCCCGTTCGGCAGtggttt                                                                                                           >  2:280913/12‑251 (MQ=255)                                                                                                                                                    gAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑AAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑AA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTAGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTgatttgattt                                                                                                      >  1:478810/1‑251 (MQ=255)                                                                                                                                                      aaa‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTc                                                                                                     >  2:229933/1‑250 (MQ=255)                                                                                                                                                                 ttCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACAAAATGGCAGGTGAAGG‑GGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGc                                                                                           <  1:7255/250‑1 (MQ=255)                                                                                                                                                                       ataatGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAt                                                                                                                                                                                        <  1:201783/151‑1 (MQ=255)                                                                                                                                                                       ataatGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAt                                                                                                                                                                                        >  2:201783/1‑151 (MQ=255)                                                                                                                                                                        aaatGTTTCACCCGGGAGCGCATCATC‑GTACAACAAAATGGCAGG‑GAAGGGAGGCATAGGG‑CA‑TAAAAAGGAAAAT‑CACA‑GACAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGt                                                                                   <  2:612736/250‑1 (MQ=255)                                                                                                                                                                          atGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCCTAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTAGTTGCTGATACGACGAATCGCTTCGACCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTAt                                                                                 >  2:236042/1‑251 (MQ=255)                                                                                                                                                                                 aTCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGAc                                                                          >  1:155885/1‑251 (MQ=255)                                                                                                                                                                                      ggAGCGAATAATCTGT‑AAACAAAATGGAAGGGGAAGG‑AGGAAAGGGT‑CA‑AACAAAGGGATAT‑AAAA‑GAAAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGa                                                                     <  2:228874/250‑1 (MQ=255)                                                                                                                                                                                                               tGGCAGGCGACGG‑CGGCATAGGT‑CA‑TAAAACTGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGCACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTCCGc                                              >  2:466781/1‑250 (MQ=255)                                                                                                                                                                                                                  cAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCt                                                                                                                                              >  2:250731/1‑151 (MQ=255)                                                                                                                                                                                                                  cAGGTGAAGG‑AGGCATAGGT‑CA‑TAAAAAGGGATAT‑AACA‑GAAAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCt                                                                                                                                              <  1:250731/151‑1 (MQ=255)                                                                                                                                                                                                                   aGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TAAA‑GACAAAAAAAACCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTATTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGgtt                                         >  1:643836/1‑251 (MQ=255)                                                                                                                                                                                                                     gTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACCACACACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGAATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGgttgt                                       >  1:119322/1‑251 (MQ=255)                                                                                                                                                                                                                             aggCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACATAGTTACGCAGGTTGTTcgccgc                                >  1:563628/1‑251 (MQ=255)                                                                                                                                                                                                                                   aGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGcc                          >  1:483542/1‑251 (MQ=255)                                                                                                                                                                                                                                         a‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCag                        >  1:64432/1‑248 (MQ=255)                                                                                                                                                                                                                                            aCAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGACTGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCgagaga                     >  2:208295/1‑249 (MQ=255)                                                                                                                                                                                                                                            aCAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAgaga                     >  1:142156/1‑249 (MQ=255)                                                                                                                                                                                                                                                 tGGATAT‑TAAA‑GACAAAAAACCCCGACGCAGCGGGTCTTTGAGCCGGGGTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTACGTTGCTGATACGACGACTCGCTTCGGCCAGCATAACTGACAGGGTCAGAGTAAGCAAGTTCGGCAGTGATTTGATTTCATCGCTCAGAGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGGTGCCagagc                     >  1:485412/1‑243 (MQ=255)                                                                                                                                                                                                                                                 gggttat‑TACA‑CAAAAAAACACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAGAGAAGATCgg              <  2:131037/247‑1 (MQ=255)                                                                                                                                                                                                                                                  ggATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAGAGAAGATCggg             >  2:215769/1‑251 (MQ=255)                                                                                                                                                                                                                                                      at‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAGAGAAGATCGGGTg           >  1:429931/1‑249 (MQ=255)                                                                                                                                                                                                                                                               aCAAAAAACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAGAGAAGATCGGGTGAGTCGCGTa  <  1:580497/251‑1 (MQ=255)                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                          CCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAA‑TATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGT‑CAACTAAATGGCAGGTGAAGG‑AGGCATAGGT‑CA‑TACAAATGGATAT‑TACA‑GACAAAAAAACCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCGGCAGTGATTTGATTTCATCGCTCAGCGGAATGGTATCGCAGACAACGACTTCATCAATTACAGAGTTACGCAGGTTGTTCGCCGCGTTGCCAGAGAAGATCGGGTGAGTCGCGTA  >  NC_000913/1260640‑1261137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑