Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,486,3860GA28.3% 79.5 / 11.1 46A89A (GCG→GCA) crpDNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base G (26/7);  new base A (0/13);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 7.62e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTC  >  NC_000913/3486137‑3486630
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    
gCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCAACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCg                                                                                                                                                                                                                                                      >  2:492786/1‑250 (MQ=255)
                 tcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGAAAGATTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTg                                                                                                                                                                                                                                    >  1:624257/1‑251 (MQ=255)
                     aTGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTg                                                                                                                                                                                                                                >  2:571768/1‑251 (MQ=255)
                      tGGTTCTTGCCTCTTTGCCACATTCATAAGTACCCACCCAAGAGCAAGCTTATTCACAAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGTTTTTATTGGCGAACTGGCCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAG‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTg                                                                                                                                                                                                                                <  1:571768/250‑1 (MQ=255)
                          tCTTGTCTCATTGCCACATTCATACCAACCCATCCAAGAGCAAGCTTATTCACAAGGGTGAAAACGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGACGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAtt                                                                                                                                                                                                                           >  1:559929/1‑251 (MQ=255)
                                                 aaGTCCCCACCCAAGACCAAGCTTATTCCCCAGGGTAAAAAAGGGGAAACGCTTTATTACATCGTTAAAGG‑TTCGGGGGCAGTGCTAAACAAAGA‑GGAAGAGGGTAAA‑GAAAGGACCCCCTCTTAT‑CTAAATAAGGGTTTTTTAATTGGCGAACTGGGCTTGTTTAAAGAGGGCAAGAAACGTAGCGAAG‑GGGT‑ACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAtt                                                                                                                                                                                                    <  2:528696/251‑1 (MQ=255)
                                                     aCCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGAGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAtt                                                                                                                                                                                                >  1:480604/1‑251 (MQ=255)
                                                         aTCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGCAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAgg                                                                                                                                                                                            >  1:462277/1‑251 (MQ=255)
                                                          tCCAAGAGCAAGTTTATTCACGAGGGAAAAAAAGGGGAAACTCTGTACTACAT‑GTTTAAGGACTTTGTGGCAGTGCTGATAAAAGA‑CGAAGAGGGAAAA‑GAAAAGTTCCTCCCCTAT‑CGGAATCAGGTTGATTTTATTGGGAAACGGGGCTTGTTGTAGGAGGGCAAGAAACGTAGCGCAG‑GGTT‑ACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAg                                                                                                                                                                                             <  2:485754/249‑1 (MQ=255)
                                                              agagCAAGCTTATTAACCAGGGCGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaa                                                                                                                                                                                                                   >  2:84437/1‑223 (MQ=255)
                                                                       ttATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAtt                                                                                                                                                                              >  1:69847/1‑251 (MQ=255)
                                                                        tatccACCAGGGTGAAAAAGGGGAACCTCTGTACAACGTTTTTAAAGG‑CTCTGTGGAGGTGCGGATAAAAGA‑CGAGGGGGGAAAA‑AAAAGGCCCCGCTCCTAT‑CTAAAGCGGGGTGATTTTATGGGCGAAGTGGGCTTGTTGGAAGGGGGCCAGAAACGTAGCGAAG‑GGGT‑AGGTGCAAAACCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt                                                                                                                                                                               <  2:669028/245‑1 (MQ=255)
                                                                             cccAGGGTGAAAAAGCGAAACCGCTGTACTAAATCGTAAAAGG‑CTCGGTGGCAGTGCTGAACAAAGA‑CGAAGAGGGAAAA‑GAAATGCCCCTCTCCTAT‑CTGATTCGGGTTGTTTTTATTGGCAACCGGGGCCTGTTTGAAGGGGCCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                                                                                                                                                        <  1:368914/250‑1 (MQ=255)
                                                                                    ggAAAAAGCGGAACCGCTGTGCTAAAGCGTAAAAGG‑CTCTGGGGCAGTGCTGAT‑AAAGAACGAAG‑GGGTAAAAGAAATGATCCTCTCCTAT‑CAGAATCAGGGTGATTTTATTGGCGAACTGGCCTTGTTGGAAGAGGGCCAGAACCGTAGCG‑TT‑GGGTTACGTGTGAAATCCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCTCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGt                                                                                                                                                                 <  1:321388/250‑1 (MQ=255)
                                                                                        aaaGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGc                                                                                                                                                             >  2:203397/1‑251 (MQ=255)
                                                                                          aGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGcac                                                                                                                                                           >  2:74788/1‑251 (MQ=255)
                                                                                                    cTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCATCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGcgcg                                                                                                                                                 >  1:243179/1‑251 (MQ=255)
                                                                                                       tactacACCGTAAAAGG‑CGCTGTGGCAGTGTTGATAAAAGA‑GAAAGAGGTTAAA‑AAAATGACCCTCTC‑TATGCTGAATCAGGGTGTTTTTATTGGCGAACGGGGCCTGTTGGAAGAGGGCCAGGACGGTAGCGCGG‑GGGT‑ACGGGAGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgtc                                                                                                                                               <  2:127601/250‑1 (MQ=255)
                                                                                                              ccttaaaaGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGTAAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGTTTTTATTGGCGAACGGGGCCTGTTGAAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑AGGTGGAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCaa                                                                                                                                       <  1:74788/246‑1 (MQ=255)
                                                                                                                    aaGG‑CTCTGTGGCAGTGCGGATCAAAGA‑CGAAGAGGGGAAA‑AAAATGTTCCTCTCCTAT‑CTGAATCAGGTTGATTTTATGGGCAAACGGGGCCTTTTTGAAGAGGGCCAGGAACGTGGCGCAT‑GGGT‑CCGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACt                                                                                                                                 <  1:224029/251‑1 (MQ=255)
                                                                                                                           atgtgGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCag                                                                                                                             >  2:520500/2‑249 (MQ=255)
                                                                                                                                gCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGaaatt                                                                                                                      >  1:164726/1‑249 (MQ=255)
                                                                                                                                gCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCACCTCT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGt                                                                                                                      >  1:599144/1‑251 (MQ=255)
                                                                                                                                 cAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATCCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTg                                                                                                                     >  1:211658/1‑251 (MQ=255)
                                                                                                                                     gcggATCAAAGA‑CGAAGAGGGAAAA‑AAAATGCCCCCCCCCTAT‑CTGAATCAGGGTGTTTTTATGGGCGACGGGGGCCTGTTGGAAGGGGGCCAGGAACGTAGCGAAG‑GGGT‑ACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGc                                                                                                                  <  1:8043/247‑1 (MQ=255)
                                                                                                                                     gCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCTTGTTGGAAGAGGGCCAGGAACGTAGCGCAG‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCa                                                                                                                 <  1:50994/251‑1 (MQ=255)
                                                                                                                                        gATCAAAGA‑CGCAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCCGAGAAAGTGGGCAAcc                                                                                                              >  1:241807/1‑251 (MQ=255)
                                                                                                                                         aTCAAAGA‑CGAAGAGGGTAAA‑GAACTGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGACGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                                                                                             >  2:575692/1‑251 (MQ=255)
                                                                                                                                           cAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTgg                                                                                                           >  1:554240/1‑251 (MQ=255)
                                                                                                                                                     agagGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGCAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGCTGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                                                                                                  >  2:577019/1‑251 (MQ=255)
                                                                                                                                                     agagGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCg                                                                                                  >  1:479092/1‑251 (MQ=255)
                                                                                                                                                            aaa‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGAc                                                                                           >  1:509790/1‑251 (MQ=255)
                                                                                                                                                              a‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACg                                                                                          >  1:232397/1‑250 (MQ=255)
                                                                                                                                                              a‑GAAATGATCCCCCCCTAA‑CTGAATCGGGGTGTTTTTATTGGCGAACTGGCCCTGTTTGAAGGGGGCCAGGAACGAGGCGCAG‑GGTT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACg                                                                                          <  2:232397/250‑1 (MQ=255)
                                                                                                                                                                           ctcCTAT‑CTGAATAGGGGTGTTTGTATTGGCGAACGGGGTCTTATTGAAGAGGCCAAGGAACGTAG‑GCTTTGGTT‑AGTTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATTCGTTTGTCTGCACAGATGGCGCGTCGTCGGCAAGTCTCTGCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGGATTGc                                                                               <  2:55196/249‑1 (MQ=255)
                                                                                                                                                                                              tgtttttATGGGCGAACGGGGCTTGTTTGAAGAGGGCCAGAAACGTCGCGCAT‑GGGT‑ACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTg                                                            <  1:203397/247‑1 (MQ=255)
                                                                                                                                                                                                           gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGCGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTAGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAgg                                              >  2:105253/1‑250 (MQ=255)
                                                                                                                                                                                                                      gttagAAGAGGGCAGGGACCGTAGTGCAT‑GGGT‑GTGTGAAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGGTTGGCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTTCACAGACGCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTc                                   <  1:319877/247‑1 (MQ=255)
                                                                                                                                                                                                                      gTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGt                                                                                                                      >  1:484462/1‑168 (MQ=255)
                                                                                                                                                                                                                       tttGAAGAGGGCCAGGAACATAGCGCAT‑GGGA‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa                                         >  2:406426/1‑244 (MQ=255)
                                                                                                                                                                                                                       ttggAAGGGGGCCAGGACCTTAGCGAAG‑GGTT‑ACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTa                                         <  1:406426/241‑1 (MQ=255)
                                                                                                                                                                                                                         tGAAGAGGGCCAGGAACGTAGCGCAG‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAc                                 <  1:496059/250‑1 (MQ=255)
                                                                                                                                                                                                                              ggggCCAGAAAGGTAGGGGAT‑GGGT‑ACGGGCAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGAc                           <  1:63216/250‑1 (MQ=255)
                                                                                                                                                                                                                                          gtggcgcAT‑GGGG‑ACGAGGAAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAAGTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCATACGCTTTGACTCATCCGGACGGTATTCAAATc               <  2:559929/248‑1 (MQ=255)
                                                                                                                                                                                                                                           tAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATc               >  1:467089/1‑250 (MQ=255)
                                                                                                                                                                                                                                              cgcAT‑GGGT‑ACGTACAAAAGCCGCCTGTGAAGTGGCTGAAAGTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGATAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAAGCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCaaaa           <  1:490106/251‑1 (MQ=255)
                                                                                                                                                                                                                                                           gTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTc  >  1:481394/1‑249 (MQ=255)
                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                    
GCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGG‑CTCTGTGGCAGTGCTGATCAAAGA‑CGAAGAGGGTAAA‑GAAATGATCCTCTCCTAT‑CTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAT‑GGGT‑ACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTC  >  NC_000913/3486137‑3486630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑