| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 119,396 | 0 | C | T | 24.6% | 128.1 / 14.5 | 69 | S39F (TCT→TTT) | ampE | protein AmpE |
| Reads supporting (aligned to +/- strand): ref base C (15/37); new base T (17/0); total (32/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.48e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCA > NC_000913/119149‑119643 | aCTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAATTTTtttct > 2:231296/1‑251 (MQ=255)aCTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTtctct < 2:279212/251‑1 (MQ=255) ccGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCTGTCCTGCATTTGATTGGGCACGGTTTCTTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAATCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGGGCTGTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAgg < 1:474319/250‑1 (MQ=255) ccGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGc < 2:455676/251‑1 (MQ=255) gggATATCGCTAAAAACAGGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCa < 2:165979/250‑1 (MQ=255) ggATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTC‑GGGCGCGCGTTAGGCAt > 1:35227/1‑251 (MQ=255) aaCATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGCCCATTATTGCg < 1:123704/251‑1 (MQ=255) tGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTC‑GGGCGCACGTTAGGCATTACCCTTATTTCGATgg > 1:251255/1‑251 (MQ=255) gCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGTGCGAGCACTGGCAGCGTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTGTTCTCGC‑GGGCGCACGTTAGGCATGACCATTTTGGCGATGGGCGTG‑a < 2:101861/251‑1 (MQ=255) gCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTC‑GGGCGCACGTTAGGCATGACCATTATTTCGATGGGGGTgg > 1:136381/1‑250 (MQ=255) ccATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑Ac > 1:291596/1‑251 (MQ=255) ttGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTTTT‑GGGGGCCCGGTTGGCATGGCCATTATTTCGGTGGGGGGG‑ACttt > 1:307742/1‑251 (MQ=255) cGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTAcgcg < 1:265217/251‑1 (MQ=255) cGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATGGTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGAGCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑ggg < 1:314476/196‑1 (MQ=255) ggATCGGAAAACCGATCCCGGACCTGCAATTGATTGAGCCCGGTTTCGTGTGATGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGATGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCCGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAACTTTTTTTCTCATGGC‑CAACTTAGGCATGCCCCTTATTTCGGTGGGCGTG‑ACttttttctttgtatgcgc > 2:361825/1‑239 (MQ=255) aTCGGAAAATCGAGACCGGTACTGCATTTGCTTGAGGACGGATTCGTGCGATGACCAGGAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTACTTTTCGAGCGCATGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAGAAACACTTTTTTCTC‑TGGCGACCGTTATGCATGCCCATTATTTGGATGGGCGTGGAGTTTTTTA‑TGTTAcgccctt > 2:556391/1‑247 (MQ=255) cGGAAAACCGATCCCGGTCCTGCATTTGAGTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCAtt < 1:46096/250‑1 (MQ=255) aaaaCCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGGTAGGCATGACCCGTATTGTCAAGGGGGTG‑ATTTTTTTTCTGTTatgccaattgc > 2:342175/1‑239 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTC‑GGGCGCACGTTAGGGATGACCATTATTGCGATGGGCGGG‑ACTTTTTTACTTTTACGCGCATTGCAgggggt > 1:530/1‑247 (MQ=255) gATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTTCTGTTACGCGCATTTCAGGGAGt > 1:348867/1‑250 (MQ=255) ccTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCaa < 1:33759/250‑1 (MQ=255) ccTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAAc < 2:85600/251‑1 (MQ=255) ccTGCATTTGATTGGGCACCGTTGCGTGTGCTGGTCAGCAAGTAGACAACATGACGCTACTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGTGCACGGTAGGCATGACCACTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCaa < 1:366724/250‑1 (MQ=255) tGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTCCAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTT‑GGGGGCCCGTTAGGCCTGACCATTATTGCGATGGGCGTG‑ACTTTTTTTCTGTTAAGCGCATTGCAGGGAGGATTGTTTAACGt > 1:92162/1‑251 (MQ=255) gCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTTATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCGCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ATTTGTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGt < 1:87656/250‑1 (MQ=255) aTTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTTTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGCG‑ACTTTTTTACTGTTTCGCGCATTGCCGGGGGTATTGTTTTACGTTcc > 1:194305/1‑251 (MQ=255) ttGATTGGGCACGGTTTCGGGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTccc < 2:494407/250‑1 (MQ=255) tGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCGCTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGTGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCt < 2:638819/250‑1 (MQ=255) tGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTAGTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCGTGAAGCGTTCGTGCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCAGGATGGCGATGCGCGTT‑ACTTTTTTACTGGTACGCGCATTGCAGGGAGTATAGTTTAACTTACCCACGCt < 2:656700/250‑1 (MQ=255) cGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAAAATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGCTGGGGGGG‑ACTTTTTTTCTGTTACGCGCAATGCAGGGAGGATTGTTTAACGTTCCCACGCTCC‑TGgtg > 1:7680/1‑251 (MQ=255) ggTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGACGCGTTCTTTCGGCGGGGGAAAAATTTTTTTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGGG‑GCTTTTTTACTTTTACGCGCCATGCAGGGAGTATTGGTCAACGTTCCCACGCTAC‑TGgtgg > 1:270405/1‑250 (MQ=255) gTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTT‑GGGCGCACCGTAGGCATGTCCATTATTTCGATGGGCCTG‑GCTTTTTTACTGTTACGCGCCATGCAGGGAGTATTTTTCAACCTTCCCACGCTAC‑TGGtgtg > 1:506980/1‑251 (MQ=255) gttcTGGTCAGCAAGGAGACAACATGACGCTATTGACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCACCGTTCCCACGCTAC‑TGGTGTGgctgct < 2:26161/247‑1 (MQ=255) gtgCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGgctgct < 1:94057/250‑1 (MQ=255) gtgCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTGACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGctgctg < 2:679335/251‑1 (MQ=255) gTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGgtg < 1:221334/236‑1 (MQ=255) gTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGGAACATTTTTTTCTC‑GGGCGCACGTTTGGCATGACCATTTTTTCGATGGGGGGT‑ACTTTTTTTCTGTTTCGCGCCTTTGAGGGGGGATTGTTCAACGTTTCCCCGCTAC‑TGgtgggatcggaagagcgg > 2:221334/1‑236 (MQ=255) aGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAAAATTTTTCTCTC‑GGGGGCACCTTAGGCATGACCATTATTTCGATGGGCGTG‑ACTTTTTTTCTGTTACGCGCATTTCAGGGAGTATTTTTCAAAGTTCCCCCGCTTA‑TGGTGTGgctgccggttgggttg > 1:426840/1‑240 (MQ=255) cAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCt < 2:136381/251‑1 (MQ=255) aaGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCtg < 1:617814/251‑1 (MQ=255) acaTGACGCTATTTACCACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGCGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGCCCCTTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGGTTGGGTTGCTGTGTATTggg > 1:213722/1‑250 (MQ=255) acaTGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGgc < 1:59569/251‑1 (MQ=255) gCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTa < 1:74130/251‑1 (MQ=255) aCAACCTTACTGGTGTTAATTTTCGAGCTCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCg < 2:307742/251‑1 (MQ=255) aaCCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTc < 2:530/251‑1 (MQ=255) ggTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCTCGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATc < 1:334510/251‑1 (MQ=255) ggTGTTAATTTTCGAGCGCCTGGTTAAGGTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGTAGCGTTCTTTCGGCTGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TTGTGTTGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCAtt < 2:543860/248‑1 (MQ=255) gtTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTC‑GGGCGCACCTTAGGCATGACCATTATTGCGGTGGGGGTG‑ACTTTTTTTCTGTTACGCGCCTTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGGTGCTGGTTGGGTTGCTGTGGATTGGGGCAGGTAAAGGTCGGCTTCATTATCAtt > 1:303480/1‑250 (MQ=255) tAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTGCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGc < 1:623135/250‑1 (MQ=255) aaTTTTCGAGCGCATGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGACGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTC‑GGGCGCACGTTTGGCCTGACCCTTTTTTCGATGGGGGTG‑GCTTTTTTTTTTTTACGCCCATTGCAGGGGGGATTTTTTAACGTTCCCACCCTAC‑TTGGGTGGGTGCTGATTTGTTTGTTGTGGTTTGGGGCCGGTGAAGTTTGTCTTTAttttctatgtt > 2:42199/1‑242 (MQ=255) aTTTTCGAGCGCCTGTTTAATTTGGGCGAGCACTGGCTGCTTGATCTTCGGCTTGAAGCGTTCTGTCGGCGGGTGTAACATTTTTCTCTC‑GGGCTCTCGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGGTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTa < 2:250633/251‑1 (MQ=255) aTTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGAGAAACATTTTTTTCTC‑GGGGACACGTTAGGCATGAACATTCTTGGGATGGGCGTG‑GCTTTTTTACTGTTACGCGCATTGCCGGGAGGATTTTTCAACGTTTCCACGCTCC‑TGGTGTGGGTGCTGACTGGGTTGCTGTGGATTGGCGCAGGGAAAATTCGTCTTCATTTTTATGCttt > 1:612459/1‑250 (MQ=255) gTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCtt < 1:112790/251‑1 (MQ=255) aaGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCAc < 2:35227/250‑1 (MQ=255) aaGTTGGGCGAGCACTGGCAGCGTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATGGTTCAACGTTCCCACGCTTC‑TGGTGTTGCTGCTGTTTGGTTTGGTGTGTATTGGTGCATGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCAc < 1:352153/250‑1 (MQ=255) gTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGTAAACATTTTTTTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGGG‑ACTTTTTTATTGTTACGCGCATTGCAGGGAGGATTGTTTAACCTTCCCACGCTAC‑TGGCGTGGCTGGTGATTGGTTTGCTGTGTTTTTGGGCCGGGAAAGTTTGTCTTCATTATTATGCTTTTTTTGCAGCTTCTTCACGt > 1:516508/1‑250 (MQ=255) gAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGGGCACGTTAGGCATGAACATTATTTCGATGGGCGTG‑ACTTTTTTACTGTTAAGCGCCTTGCAGGGAGTTTTGTTCAACGTTCCCCCGCTAC‑TGGTGTTGCTGGTTGTTTGTTTGCTGTGTTTTGGCGCAGGGAAAGTTCTTTTTCCTTATCATTCTTATCTGACCGCTGCTTTCCGTAAttatt > 1:534930/1‑246 (MQ=255) cTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTTTCTC‑GGGCGCACGTTAGGCATGACCATTATTGTGATGGGCGGG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGGATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGTTTGGTTCGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTTTCATGCTTTTCTGACAGCTGCTTCACGTAATGATAGCCATg > 1:480626/1‑251 (MQ=255) gCAGCTTGCTCATCGTCTTGAAGCGTTCTTTCGGCGGGTGACACAATTTTCTCTC‑GGGGGGACGCTAGGCATGAACCTTATTGCGACGGGGGGG‑CCTTTTTTTTTGTTTCGCGCATTGCAGGGGGGATTGTTTACCGTTTCCACGC‑ACATGGGGTGGCTGCTGGTTTGTTTGTTGTGGTTTTGCGGAGGTAAAATTTCTCTTCATTTTCATTCTTTTTTGACAGCTGCTTCCCGGAATGATAggccttacc > 2:643657/1‑243 (MQ=255) cTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTTTCTC‑GGGGGCACGGTAGGCATGAACATTATTGCGATGGGCGGG‑ACTTTTTTACTGGTAAGCGCATTGCAGGGAGGATTGTTTAACGTTCCCACGCTAC‑TGGTGTGGGTGCTGTTTGGTTTGCTGTGTATTGGCGCAGGGAAAGTTTGTCTTCATTATCATGCTTATCTGACAGCTGGTTCACGGCATGATAACCATGCCCCTGc > 1:95650/1‑251 (MQ=255) cTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGGGAAAACTTTTTCTCTC‑GGGCGCACGTTAGGCATGAACCTTATTGCGCTGGGGGTG‑ACTTTTTTTCTGTTTCGCGCATTGCAGGGGGTATTTTTTAACGTTTCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTTCTGTGTATTGTCGCCGGGAAAGTTAGTCTTCTTTTTCCTTCTTATTTGACAGCTGCTTCACGGAATGATAGcactgccccggc > 2:246178/1‑240 (MQ=255) atcatcGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACg < 2:291596/251‑1 (MQ=255) atcGTCTTGAAGCGTTCTTTCGGCGGGGGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTTTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTTCTGGGTATTGGGGCAGGTAAAGGTCGTCTTCATTATCCTGCTTATCTGACAGCTGCTTCACGGAATGATAACCATGCCCGTGCCACGAtg > 1:314919/1‑251 (MQ=255) tGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGAtggctg < 1:7388/248‑1 (MQ=255) ggcggGTGAAACATTTTTCTCTC‑GGGCGCACGGTAGGCAGGACCCTTGTTGCGATGGGCGTG‑ACTTTTTTAATGTTACGCGCATTGCAGGGAGTGTTGTTCAACGTTCCCACGCTGC‑TGGTGTGGGTGCTGATTGGGTTGCTGGGTATTGGCGCAGGTAAAGTGCGTATTCATTTTCCTGCTTATCTAACAGCTGCTTCAAGTAATTATAGCCATGCACGTGCCCCGATGGCTGGCGAAccacccattctt > 2:366324/1‑240 (MQ=255) ggcggGGGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTTGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTCATGATAACCATGCCCGTGCCACGATGGCTGGCTAACTCACCCTGAtt > 2:628468/1‑251 (MQ=255) gcggGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCCTGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTc > 2:28589/1‑251 (MQ=255) cATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTccc < 2:194305/251‑1 (MQ=255) aTTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGt < 2:529767/151‑1 (MQ=255) aTTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGt > 1:529767/1‑151 (MQ=255) ttCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTTGCGAACTCACCATGATTCACGGCGTCCCGGCa < 2:339344/250‑1 (MQ=255) | ACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGACGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTTGAAGCGTTCTTTCGGCGGGTGAAACATTTTTCTCTC‑GGGCGCACGTTAGGCATGACCATTATTGCGATGGGCGTG‑ACTTTTTTACTGTTACGCGCATTGCAGGGAGTATTGTTCAACGTTCCCACGCTAC‑TGGTGTGGCTGCTGATTGGTTTGCTGTGTATTGGCGCAGGTAAAGTTCGTCTTCATTATCATGCTTATCTGACAGCTGCTTCACGTAATGATAGCCATGCCCGTGCCACGATGGCTGGCGAACTCACCATGATTCACGGCGTCCCGGCA > NC_000913/119149‑119643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |