Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913766,7160GT40.0% 7.5 / 10.6 25A245S (GCG→TCG) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
Reads supporting (aligned to +/- strand):  ref base G (9/6);  new base T (10/0);  total (19/6)
Fisher's exact test for biased strand distribution p-value = 5.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGC‑AA‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCT  >  NC_000913/766474‑766966
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                
tGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAATGC‑AA‑ACCCCTGGATTTTTTCGGCTgg                                                                                                                                                                                                                                                           >  1:168121/1‑249 (MQ=255)
                                                     aaCGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGGTTAACGGCGGAACAACTCCAAAATGC‑AA‑ACCCCTGGTTTTTTTCCGCTGGAGTGGCCCCCAAAGAGAGTGAGGGTTTTTAT‑gggcttccccccgctttactgac                                                                                                                                                                                                    >  1:389865/1‑230 (MQ=255)
                                                        cgccaatctcccGTCTGCGGAAGGGCCTGGCGGGGGGGTGTTGCTCACTCTTATGTGGCGACGGAATATCTGAAAAAAGCCGGACGAAGCCGCGGTTTAAA‑GTTTGAGTTGAAAAAACAAGGCGGAAGCGTTTTGGAAGGGTGTTTATGGGGGGAGCAATTAAATGGTGC‑AG‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGTAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGt                                                                                                                                                                                                <  1:620071/240‑1 (MQ=255)
                                                                            aaCGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACAACTCAACAATGG‑AA‑CCGCCTGTATTTTTTCGGCTGAAAGAGCCATCAAGGAGAGTGGGGGGTTTAAT‑TGCATTTCCCCGCCTTTCTG‑GCCTGTTGCCCGGCCGAAT‑CGCCAtt                                                                                                                                                                           >  1:381602/1‑250 (MQ=255)
                                                                                           gggTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGC‑AA‑CCGCCTGTATTTTTTCGGCTGGAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCCGAAGCG‑TTGGTcc                                                                                                                                                           >  1:210800/1‑251 (MQ=255)
                                                                                                 tGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTACCGGCATTGAAGGGCGTTTAACGGCGGATTAAATCAATAGTGCCAA‑CC‑CCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGCGAGAGCGTTTTAAT‑GGAATTTCCGCCC‑TTTCAGTGACTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCcaacc                                                                                                                                                       >  1:660975/1‑248 (MQ=255)
                                                                                                                                 aaaaaGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGAACAAATCAATAAAGC‑AA‑CCGCCCGTATTTTTTCGGGTGAAGCCGCCACCCAGGGGAGGGAGGGGTTTAAA‑GGGATTCCCGCGC‑TTTTAGTGCGTGTTTTCGCACCGATTACGC‑ATGCAGGAGCGGTTGTTCCACCAA‑CGC‑TTACCCTcccgacgaacgaggtgacccgc                                                                                                                    >  2:547835/1‑230 (MQ=255)
                                                                                                                                   aaaGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGCTCAACTCAACAGTCC‑CA‑CCGCCTGTATTTTTTCGGCTGAAGTCGCCACCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCCTGCCAAAGCG‑TTGATCCCACAAGCGC‑TTACCCTCAAGCGTAGCGGTGAGACGcgtac                                                                                                                  >  1:343774/1‑251 (MQ=255)
                                                                                                                                       ccGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CATGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGC‑CA‑CCGCCTGTATTTTTGCGGCTGAAGGCGCCATCAAGGAGAGTGAGCGGTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATTCAACAAGCGC‑TTACCCTCCAGCGTAGCGATGAGACGcgtacc‑gt                                                                                                              >  1:67245/1‑250 (MQ=255)
                                                                                                                                         ggACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATCGTGC‑AA‑CCGCCTGTATTTTTTCGGCTGCAGGCGCCCTCAAGGAGAGTGAGCGGTTTTAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCCATT‑CGCCATGCCGGAGCG‑TTGATCCAACAAGCGC‑TTCCCCCCCAGCGGAGCGGTGAGACGcgtacc‑gtac                                                                                                            >  1:304539/1‑250 (MQ=255)
                                                                                                                                                 aCTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGC‑CA‑CCGCCTGTATTTTTTCGGCTGAAGTCGCCATCAAGGGGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGGCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGAt                                                                                                    >  1:143615/1‑250 (MQ=255)
                                                                                                                                                          aaaTGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGGGC‑AA‑CCGCCTGTATTTTTGCGGCTGAAGGCGCCATCAAGGGGAGTGAGCGTTTTTAT‑GGCATTCCCGCGC‑TTTCCGTGCCTGTTGCCGAGCCGATT‑CGCCCTGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCCCAAGCGGAGCGGTGAGACGCGTACC‑GTAAAGCCAGATAcagatcggg                                                                                          >  1:10536/1‑243 (MQ=255)
                                                                                                                                                                            a‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCCACTCAATAGTGC‑AA‑CCGCCCGTATTTTTTCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTTAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑CTACCCTCAAGCGGAGCGGTGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACgg                                                                        >  1:323110/1‑251 (MQ=255)
                                                                                                                                                                                    gcTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCCATAGTGC‑AA‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCCTTTCCGCGC‑TTTCAGTGCCTGTTGCCGCGCCGATT‑CGCCATGCAGAAGCG‑TTGGTCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTCCC‑GTACAGCAAGATACGCAACCGGGGAAAAGTGGCAAAAAGGAGCTGa                                                                  >  1:22737/1‑250 (MQ=255)
                                                                                                                                                                                          ggCATTGAAGGGCGTTTAACGGCGGATCAACTTAATAGTGC‑AA‑CCGCCTGTATTTTTTCGGCTGAAGGCGCCATCCAGGGGAGGGAGCGGTTTAATTGGC‑TTCCCCCGC‑CTTCCGTGCCTGTTGCCGAGCCGGTT‑CGCCCTGCAGAAGCGGTTG‑TCCAACAAGCGCCTTACC‑TCAACCGGAGCGGTTGGACGCGGACCCGTA‑AGCCAGAGACGCAACCGGGGCAAAGTGTCAAAAAGGAGGTGAAAAAggg                                                           >  2:510124/1‑250 (MQ=255)
                                                                                                                                                                                           gtgtaggggggatttgagcgcaggATCAACTCAATAGCGC‑AC‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCa                                                          <  2:168121/229‑1 (MQ=255)
                                                                                                                                                                                              ttGAAGGGCGTTTAACGGCGGCTCAACTCAAAAGTGC‑AA‑ACACCTCTATTTTTGCGGCTGAAGACGCCATCCAGGAGAGGGAGCGCTTTTAT‑GGCATTCCCGCGC‑CTTCAGTGCCTGGTGCCGAGCCGATT‑CTCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGCTGAGACGCGTACC‑GTACAGCAAGAAACGCAAACGGTGAAAAGTGTCAAAAAGGAGCTGAAACAGGCACTg                                                       >  1:414153/1‑251 (MQ=255)
                                                                                                                                                                                                              gggcgaaaaaccaaaaaGTGC‑AATCCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATGCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCGGAAACAGGCACTGTTGAGCGGAATctct                                        <  1:570395/235‑1 (MQ=255)
                                                                                                                                                                                                                        cTCAATAGTGC‑AA‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATTAAGGAGAGTGAGCGTATTAAT‑GGCATTCCCGCGA‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGAGCAAAGTGTCAACACGGCGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTAcc                             >  1:81902/1‑251 (MQ=255)
                                                                                                                                                                                                                            aTAGTGC‑AA‑CAGCCTGTATTTTTGCGGCTGAAGTCGACATCAAGGCGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGCGCCGATT‑CGCCATGCAGACGCG‑TTGATCCAACAAGCGC‑TTACCCACAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACGGTTg                         >  2:662099/1‑251 (MQ=255)
                                                                                                                                                                                                                              aGTGC‑AA‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGCGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGCGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGAt                       >  1:613523/1‑251 (MQ=255)
                                                                                                                                                                                                                                     a‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCCGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGCGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGCATCCCTTTTGCCGTACCGTTGATTGTcgg                 >  2:298389/1‑250 (MQ=255)
                                                                                                                                                                                                                                               aTTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCa         <  1:2620/250‑1 (MQ=255)
                                                                                                                                                                                                                                               aTTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCAc        <  1:674359/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   ttGCGGCTGAAGTCGCCATCAAGGAGAGTGTGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCACCCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGt     <  2:381602/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     gCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTACT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCt  >  1:39650/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     gCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAAc                                                               >  2:361407/1‑190 (MQ=255)
                                                                                                                                                                                                                                                     gCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTggtggcggcagcacagaa    >  1:38457/1‑232 (MQ=255)
                                                                                                                                                                                                                                                     gCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGAGGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAAc                                                               <  1:361407/190‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                
TGCTCTCGGCGCTGGATGACAAGGGAGGCACGCAACCTTCTGCCTCTTTTTCCAACGCGCCAACTATCGTCTGCGTAACGGCCTGTCCGGCGGGTATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAA‑CAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGC‑AA‑CCGCCTGTATTTTTGCGGCTGAAGTCGCCATCAAGGAGAGTGAGCGTTTTAAT‑GGCATTCCCGCGC‑TTTCAGTGCCTGTTGCCGAGCCGATT‑CGCCATGCAGAAGCG‑TTGATCCAACAAGCGC‑TTACCCTCAAGCGTAGCGATGAGACGCGTACC‑GTACAGCAAGATACGCAACCGGTGAAAAGTGTCAAAACGGAGCTGAAACAGGCACTGTTGAGCGGAATCTCTTTTGCCGTACCGTTGATTGTCGCGGGGGGCACGGTGCT  >  NC_000913/766474‑766966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑