Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,245,2340GT41.7% 13.2 / 11.0 24L25F (TTG→TTT) ycgYuncharacterized protein YcgY
Reads supporting (aligned to +/- strand):  ref base G (1/13);  new base T (10/0);  total (11/13)
Fisher's exact test for biased strand distribution p-value = 5.61e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA‑CTCCAGAAAGCAAAAAAACGTTGATGAG‑TGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAG  >  NC_000913/1244994‑1245467
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
ggCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGATAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTa                                                                                                                                                                                                                                     <  2:196362/251‑1 (MQ=255)
  cGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCtt                                                                                                                                                                                                                                      <  2:150119/248‑1 (MQ=255)
  cGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGCTGCGCtttg                                                                                                                                                                                                                                    >  1:150119/1‑248 (MQ=255)
                      gCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGGGCTTTTTTAAAATTTAAAATTTATCa                                                                                                                                                                                                                >  1:608567/1‑250 (MQ=255)
                            cGCTTACACATCATAACGGATACTCGACTCCTGCGTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTTTTTCGTTGTTTTTTGTGTGATTCAGAAAATAAATATTTTAGACATTAGGCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTGGGCTGCGCGTATTGAACATTTAGAATTGATCAAT‑CCTTc                                                                                                                                                                                                        <  1:57789/251‑1 (MQ=255)
                                   acaTCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTGTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTTGCAGa                                                                                                                                                                                                 <  2:366660/251‑1 (MQ=255)
                                       cAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTaa                                                                                                                                                                                             <  2:359951/251‑1 (MQ=255)
                                               ataCTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGATCCCGGAAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTCGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGGTTAATGAACATTTTTAATTATTTAAA‑CCTTCTGGGAGATTAAACCaaaa                                                                                                                                                                                      >  1:329651/1‑250 (MQ=255)
                                                 aCTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTTGTGCGCTTATTGAACATTTAGAAATGATTAAA‑CCTTCTGGCCGAGTAAGCCCAAATA‑c                                                                                                                                                                                  >  2:79163/1‑251 (MQ=255)
                                                                                              gATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAAtt                                                                                                                                     <  1:309506/251‑1 (MQ=255)
                                                                                                                   ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTATTTGCTGGGCTTATTTAAAAATTTGAAATGATCAAT‑CCTTCTTGCAGAATAAACCCAAAAA‑CCCGCTCGGCAATGCTTTCGTTACGCGGAAAGGGAAGGTCTGAATTTTTTTTTTTGtttttaaaatt                                                                                                                >  1:454752/1‑242 (MQ=255)
                                                                                                                      gTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTctct                                                                                                             <  2:391499/251‑1 (MQ=255)
                                                                                                                                    aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTTAACATTTAAAATTGATCAAT‑CCTTCTGGCAGATTTAACCACAATA‑CCCCTTTGGCCATGCTTCCCTTTCGCGAAGAGGAATGGTCTTCATTCTTTTTTTTGTTTTTAAATTTTCTGGAGTGTCTGGAtt                                                                                               >  1:370779/1‑251 (MQ=255)
                                                                                                                                            ttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGat                                                                                        <  1:136665/250‑1 (MQ=255)
                                                                                                                                                 tattGTTGCGTTGTTGTTGGTGTGATTAAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATGTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGAttatta                                                                                   <  2:370779/250‑1 (MQ=255)
                                                                                                                                                             gttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA‑CTCCAGa                                                                     <  1:458709/251‑1 (MQ=255)
                                                                                                                                                                                   taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTGAACATTTTGAATTTGTCAAT‑CCTTTTTGCCGATTAAGCCAAAATA‑ACCGTTCGGGCATGCTTCAGTTACGCGAAGAGGAATGGGCTGAATTCTTTTTTTTGTTTCTAAATTCTCTGGGGGGTCTGGGTTATTTGTTTTTTAATTTAA‑CTCCAGAAAGCAAAAAAAAGTTGATGAgg                                               >  1:422218/1‑250 (MQ=255)
                                                                                                                                                                                               aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTgg                                                                                                  <  1:361723/189‑1 (MQ=255)
                                                                                                                                                                                                     gCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGATTTGACTGCCCCTCTTTAAAAATTAGAAATGATCAATACCTT‑TGGCAGATTTAACCAAAATAACCCTTTCG‑CCATGCTTTAGTTAAGCCAAGAAGAATGGTTTGAATTTTTTTTTTGGTTTCTTAATTCTTTTGGGGGTCTGGGTTATTTGATTTTTTATTTAAATTCCAAAAAGCAAAAAAAGGTT‑ATGAGGTGAA‑ACCGCAAAAATAtt                            >  1:673675/1‑251 (MQ=255)
                                                                                                                                                                                                           cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGGGCTTTTTAAACATTTAGGATTGATCAAA‑CCTTCTGGCAGCTTACGCCACAAAA‑CCCGATCGGCAATGGCTCAGTTACGCGGCGGGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGGTTTTTTATTTAA‑CTCCAGAAAGCAAAAAAACGTTGATGAG‑TGAACACCGTAACAAAATTCAGGt                       >  1:77593/1‑250 (MQ=255)
                                                                                                                                                                                                            aGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGTGCGCTTTTTGTACATTTATAATTTATCAAT‑CCCTCTGGCAGATTAAACCAAAAAA‑ACCGTTCGGCAATGCTTTAGTTACGGGAAGAGGAATGGGCTGAATTCTTTTTTTTGTTGCTAAAATTTCTGGGGTGTTTGGGTTATGTGGTTATTAATTTAA‑ATCCAGAAAGCAAAAAAACGTTGATGAG‑GGAACACCCAAACAAAATTCAGGt                       >  1:235671/1‑249 (MQ=255)
                                                                                                                                                                                                              gCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGCTGGGCCTTTTTTACATTTTGAATTTATTAAT‑CCTTCTTGCCGATTACAACAACATA‑CCCGATCGGCAATGCTTCAGTTACCCGACGAGGAATGGTCTGAATTTTTTTTTTTGTTGCTAAATTTTTTTGAGGGGTTGGATTTTTTGGTTATTAATTTTA‑CTCCACAAAGCAAAAAAAAGTTGTTTAG‑GGAAAACCGTAACAATATTTAGGGAGc                    >  1:677700/1‑250 (MQ=255)
                                                                                                                                                                                                                           ggCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA‑CTCCAGAAAGCAAAAAAACGTTGATGAG‑TGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTtat      <  2:82658/251‑1 (MQ=255)
                                                                                                                                                                                                                               gATAGTGAGGCGGGTTTGGCTGCGTTTATTGAACATTTAGAATTGATCAAT‑CTTTCTGGCAGATTAAGTCAAAATA‑TCCGTTCGGCAATGCTTCAGTTAAGCGACGAGGAATGGTCTGAATTCTTTTTTTGGTTGTTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA‑CTACAGAAAGCAAAAAAACGTTGATGAG‑TGACCACCGTAACAATATTCAGGTAgaaacggatgttttttatag  <  2:266634/251‑20 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             
GGCGGATAGCGATACAGATGCCGCTATCCGCTTTCACATCAGAACGTATACTCGACACCTGCTTTACGGGTGAAAAAAATCAATATAGCACTTCGATATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAAT‑CCTTCTGGCAGATTAAGCCAAAATA‑CCCGTTCGGCAATGCTTCAGTTACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATTATTAATTTAA‑CTCCAGAAAGCAAAAAAACGTTGATGAG‑TGAACACCGTAACAATATTCAGGTAGCAATTGATGCTTTATATAG  >  NC_000913/1244994‑1245467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑