breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 51,290 C→T G106D (GGC→GAC)  apaG ← DUF525 domain‑containing protein ApaG
RA 93,591 C→T G142G (GGC→GGT murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
RA 135,764 G→A A234V (GCA→GTA)  speE ← spermidine synthase
RA 177,404 A→T L74Q (CTG→CAG)  yadS ← PF03458 family protein YadS
RA 186,539 C→T T704T (ACG→ACA glnD ← protein‑PII uridylyltransferase/uridylyl‑removing enzyme
RA 202,615 C→T S19L (TCA→TTA)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
RA 220,524 C→T V135V (GTG→GTA metQ ← L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
RA 225,731 G→A intergenic (+156/‑28) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 225,736 C→T intergenic (+161/‑23) alaV → / → rrlH tRNA‑Ala/23S ribosomal RNA
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 280,709 C→T W9* (TGG→TGA yagB ← orphan antitoxin YagB
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
JC JC 332,367 IS2 (–) +5 bp [pdeL] [pdeL]
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 339,986 G→A R73Q (CGA→CAA)  yahH → putative uncharacterized protein YahH
RA 356,036 G→A G372E (GGG→GAG)  codB → cytosine transporter
RA 364,850 G→A Q486* (CAG→TAG)  lacZ ← beta‑galactosidase
MC JC 392,967 Δ5,466 bp IS3‑mediated yaiT[yaiW] yaiT, iprA, ampH, sbmA, [yaiW]
RA 402,739 G→A L331L (TTG→TTA phoA → alkaline phosphatase
RA 431,286 G→A A243V (GCT→GTT)  tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 431,591 G→A R141R (CGC→CGT tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 435,874 G→A A81T (GCC→ACC)  thiL → thiamine monophosphate kinase
RA 437,421 G→A L239F (CTC→TTC)  yajO ← 1‑deoxyxylulose‑5‑phosphate synthase YajO
RA 441,092 G→A P4S (CCG→TCG)  ispA ← geranyl diphosphate/farnesyl diphosphate synthase
RA 441,907 G→A S120N (AGC→AAC)  thiI → tRNA uridine 4‑sulfurtransferase
RA 447,020 G→A A229V (GCT→GTT)  cyoE ← heme O synthase
RA 448,014 G→A S11S (TCC→TCT cyoD ← cytochrome bo3 subunit 4
RA 451,013 G→A P200S (CCC→TCC)  cyoA ← cytochrome bo3 subunit 2
RA 459,694 G→A E269E (GAG→GAA lon → Lon protease
RA 460,576 G→A K563K (AAG→AAA lon → Lon protease
RA 464,112 G→A G59D (GGC→GAC)  ybaV → helix‑hairpin‑helix 3 family protein
RA 466,723 G→A A197V (GCA→GTA)  ybaE ← uncharacterized protein YbaE
RA 467,244 G→A T23T (ACC→ACT ybaE ← uncharacterized protein YbaE
RA 478,694 G→A intergenic (‑77/+87) pdeB ← / ← ylaC c‑di‑GMP phosphodiesterase PdeB/putative inner membrane protein
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 509,517 C→T E621E (GAG→GAA copA ← Cu(+) exporting P‑type ATPase
RA 631,537 G→A W548* (TGG→TGA cstA → pyruvate transporter CstA
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 698,679 G→A P167S (CCA→TCA)  asnB ← asparagine synthetase B
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 869,418 G→A R3K (AGA→AAA)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 871,045 G→A V27I (GTC→ATC)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 873,115 G→A R46H (CGC→CAC)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 884,344 G→A G224G (GGG→GGA mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 897,642 G→A V187I (GTC→ATC)  potI → putrescine ABC transporter membrane subunit PotI
RA 909,012 G→A L95L (CTG→TTG)  ltaE ← low‑specificity L‑threonine aldolase
RA 914,713 C→T A49T (GCG→ACG)  lysO ← L‑lysine/thialysine exporter
RA 924,350 C→T P363S (CCG→TCG)  clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 987,159 T→A intergenic (‑177/+426) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,070,014 A→G L114P (CTG→CCG)  rutF ← FMN reductase RutF
RA 1,106,559 G→A A69T (GCA→ACA)  clsC → cardiolipin synthase C
RA 1,109,816 G→A G161D (GGT→GAT)  opgG → osmoregulated periplasmic glucans biosynthesis protein G
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,111,867 G→A W335* (TGG→TGA opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,113,805 G→A *76* (TGA→TAA)  yceK → DUF1375 domain‑containing lipoprotein YceK
RA 1,115,668 G→A R305R (CGC→CGT lpxL ← lauroyl acyltransferase
RA 1,125,322 G→A S2F (TCC→TTC)  mdtH ← multidrug efflux pump MdtH
RA 1,127,608 G→A G268D (GGC→GAC)  yceM → putative oxidoreductase YceM
RA 1,132,149 G→A G99D (GGC→GAC)  flgD → flagellar biosynthesis, initiation of hook assembly
RA 1,132,865 G→A G98R (GGA→AGA)  flgE → flagellar hook protein FlgE
RA 1,135,235 G→A Q169Q (CAG→CAA flgG → flagellar basal‑body rod protein FlgG
RA 1,136,053 G→A V164M (GTG→ATG)  flgH → flagellar L‑ring protein
RA 1,140,714 G→A A228T (GCG→ACG)  flgL → flagellar hook‑filament junction protein 2
RA 1,150,616 C→T L297L (CTG→TTG)  fabD → [acyl‑carrier‑protein] S‑malonyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,220,797 G→A intergenic (+1596/+1508) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,276,230 C→T P463P (CCG→CCA narX ← sensor histidine kinase NarX
RA 1,300,882 G→A intergenic (+335/‑300) insH21 → / → oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
MC JC 1,302,147 Δ5 bp coding (966‑970/1632 nt) oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,315,384 G→A Y129Y (TAC→TAT yciF ← DUF892 domain‑containing protein YciF
RA 1,317,129 G→A Q32* (CAG→TAG)  trpA ← tryptophan synthase subunit alpha
RA 1,322,325 G→A L208F (CTC→TTC)  trpE ← anthranilate synthase subunit TrpE
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,356,932 G→A T60I (ACC→ATC)  sapA ← putative periplasmic binding protein SapA
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,366,433 G→A G295D (GGC→GAC)  puuE → 4‑aminobutyrate aminotransferase PuuE
RA 1,400,978 C→T G266S (GGT→AGT)  abgT ← p‑aminobenzoyl glutamate:H(+) symporter
MC JC 1,428,795 Δ115,337 bp IS5‑mediated lomRnarU 104 genes
RA 1,603,321 C→T L101L (CTC→CTT lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,604,223 C→T G59G (GGC→GGT lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,604,840 C→T S265F (TCC→TTC)  lsrD → Autoinducer‑2 ABC transporter membrane subunit LsrD
RA 1,605,533 C→T S161S (AGC→AGT lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,606,464 C→T A122V (GCC→GTC)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,608,089 C→T A248A (GCC→GCT tam → trans‑aconitate 2‑methyltransferase
JC JC 1,633,913 IS5 (–) +4 bp coding (292‑295/591 nt) pinQ → putative recombinase PinQ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,743,057 G→A D62D (GAC→GAT ribC ← riboflavin synthase
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,888,498 C→T G417S (GGT→AGT)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
JC JC 1,907,451 IS2 (+) +5 bp coding (137‑141/144 nt) yobF ← DUF2527 domain‑containing protein YobF
RA 1,919,728 C→T T740I (ACT→ATT)  letB → lipophilic envelope spanning tunnel
RA 1,978,245 A→C intergenic (‑48/+273) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 1,983,190 G→A G117G (GGC→GGT araH ← arabinose ABC transporter membrane subunit
RA 1,986,333 A→G intergenic (‑205/‑592) araF ← / → ftnB arabinose ABC transporter periplasmic binding protein/putative ferritin‑like protein
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,174,817 IS1 (+) +9 bp coding (225‑233/453 nt) gatA ← galactitol‑specific PTS enzyme IIA component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,195,035 G→A A246T (GCG→ACG)  metG → methionine‑‑tRNA ligase
RA 2,223,182 G→A G333D (GGC→GAC)  dld → quinone‑dependent D‑lactate dehydrogenase
RA 2,223,909 G→A intergenic (+9/+29) dld → / ← pbpG quinone‑dependent D‑lactate dehydrogenase/peptidoglycan DD‑endopeptidase PbpG
RA 2,225,926 G→A P42P (CCG→CCA yohD → DedA family protein YohD
RA 2,248,732 G→A intergenic (‑200/+5) lysP ← / ← yeiE lysine:H(+) symporter/DNA‑binding transcriptional regulator YeiE
RA 2,249,800 G→A G28G (GGG→GGA yeiH → PF03601 family inner membrane protein YeiH
RA 2,251,644 G→A A269T (GCA→ACA)  nfo → endonuclease IV
RA 2,270,933 C→T P70S (CCG→TCG)  pdeN → putative c‑di‑GMP phosphodiesterase PdeN
RA 2,286,554 C→T pseudogene (2361/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,415,485 G→A G247S (GGC→AGC)  pta → phosphate acetyltransferase
RA 2,435,922 C→T G243D (GGT→GAT)  usg ← putative semialdehyde dehydrogenase Usg
RA 2,454,553 G→A intergenic (‑2217/+530) yfcS ← / ← yfcV putative fimbrial chaperone YfcS/putative fimbrial protein YfcV
RA 2,473,881 G→A R93H (CGC→CAC)  yfdP → uncharacterized protein YfdP
RA 2,474,474 G→A G148D (GGC→GAC)  yfdQ → DUF2303 domain‑containing protein YfdQ
RA 2,474,924 G→A intergenic (+68/‑60) yfdQ → / → yfdR DUF2303 domain‑containing protein YfdQ/5'‑deoxynucleotidase
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,486,855 G→A A828T (GCG→ACG)  evgS → sensor histidine kinase EvgS
RA 2,535,852 C→T L7L (CTG→TTG)  crr → Enzyme IIA(Glc)
RA 2,536,118 C→T D95D (GAC→GAT crr → Enzyme IIA(Glc)
RA 2,552,984 G→A K211K (AAG→AAA amiA → N‑acetylmuramoyl‑L‑alanine amidase A
RA 2,555,841 G→A A187V (GCA→GTA)  eutL ← putative structural protein, ethanolamine utilization microcompartment
RA 2,557,071 G→A A76V (GCC→GTC)  eutC ← ethanolamine ammonia‑lyase subunit beta
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,600,716 G→A R80H (CGT→CAT)  bcp → thiol peroxidase
RA 2,602,942 G→A G375G (GGG→GGA hyfB → hydrogenase 4 component B
RA 2,603,828 G→A A671T (GCC→ACC)  hyfB → hydrogenase 4 component B
RA 2,609,438 G→A E318K (GAA→AAA)  hyfG → hydrogenase 4 catalytic subunit HyfG
RA 2,622,358 G→A G42D (GGC→GAC)  purN → phosphoribosylglycinamide formyltransferase 1
RA 2,638,495 C→T G53D (GGT→GAT)  bamB ← outer membrane protein assembly factor BamB
RA 2,639,290 C→T intergenic (‑7/+11) yfgM ← / ← hisS ancillary SecYEG translocon subunit/histidine‑‑tRNA ligase
RA 2,644,140 C→T W48* (TGG→TGA rlmN ← 23S rRNA m(2)A2503 methyltransferase/tRNA m(2)A37 methyltransferase
RA 2,644,810 C→T V19I (GTC→ATC)  ndk ← nucleoside diphosphate kinase
RA 2,646,753 G→A S191S (TCC→TCT pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,647,248 C→T W26* (TGG→TGA pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,647,438 C→T R1617Q (CGG→CAG)  yfhM ← alpha2‑macroglobulin
RA 2,649,752 C→T G846S (GGT→AGT)  yfhM ← alpha2‑macroglobulin
RA 2,653,730 C→T intergenic (‑275/‑125) timR ← / → ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
RA 2,654,454 G→A T160T (ACC→ACT sseB ← protein SseB
RA 2,656,285 C→T S25N (AGC→AAC)  pepB ← aminopeptidase B
RA 2,661,953 C→T G60E (GGA→GAA)  iscR ← DNA‑binding transcriptional dual regulator IscR
RA 2,663,356 C→T intergenic (‑33/‑86) trmJ ← / → suhB tRNA Cm32/Um32 methyltransferase/Nus factor SuhB
RA 2,666,527 C→T L365L (CTG→TTG)  csiE → stationary phase‑inducible protein CsiE
RA 2,670,240 C→T R403R (CGC→CGT hcaE → putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
RA 2,674,066 C→T G208D (GGT→GAT)  yphB ← putative aldose 1‑epimerase YphB
RA 2,675,846 C→T S327N (AGC→AAC)  yphD ← putative ABC transporter membrane subunit YphD
RA 2,676,664 C→T M54I (ATG→ATA yphD ← putative ABC transporter membrane subunit YphD
RA 2,678,218 G→A G48G (GGC→GGT yphE ← putative ABC transporter ATP‑binding protein YphE
RA 2,685,310 G→A G66G (GGC→GGT glyA ← serine hydroxymethyltransferase
RA 2,692,949 G→A G865G (GGC→GGT purL ← phosphoribosylformylglycinamide synthetase
RA 2,698,650 G→A intergenic (‑100/‑109) pgpC ← / → yfhH phosphatidylglycerophosphatase C/putative DNA‑binding transcriptional regulator YfhH
RA 2,713,679 C→T H262Y (CAT→TAT)  srmB → ATP‑dependent RNA helicase SrmB
RA 2,718,545 G→A intergenic (‑17/‑190) yfiF ← / → trxC putative methyltransferase YfiF/reduced thioredoxin 2
RA 2,722,605 G→A E885K (GAA→AAA)  patZ → peptidyl‑lysine N‑acetyltransferase
RA 2,725,233 C→T G172R (GGA→AGA)  kgtP ← alpha‑ketoglutarate:H(+) symporter
RA 2,726,177 G→T noncoding (12/120 nt) rrfG ← 5S ribosomal RNA
RA 2,736,417 C→T A91V (GCC→GTC)  bamD → outer membrane protein assembly factor BamD
RA 2,777,980 Δ2 bp intergenic (+198/+165) ypjF → / ← ypjA toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 2,777,984 Δ1 bp intergenic (+202/+162) ypjF → / ← ypjA toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 2,796,259 G→A A196T (GCG→ACG)  glaR → DNA‑binding transcriptional repressor GlaR
RA 2,912,293 G→A Y453Y (TAC→TAT relA ← GDP/GTP pyrophosphokinase
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,928,781 C→T P185S (CCG→TCG)  sdaC → L‑serine:H(+) symporter SdaC
RA 3,053,212 C→T G103R (GGA→AGA)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol 4‑hydroxylase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,241,019 T→A F359Y (TTC→TAC)  sstT → serine/threonine:Na(+) symporter
RA 3,279,461 A→G H183R (CAT→CGT)  kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,413,728 G→A intergenic (‑263/‑136) envR ← / → acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,441,060 T→C K206E (AAG→GAG)  rpsD ← 30S ribosomal subunit protein S4
RA 3,476,285 G→A H52H (CAC→CAT yheO ← DNA‑binding transcriptional regulator YheO
RA 3,510,832 C→T A49T (GCG→ACG)  yhfX ← putative PLP‑binding protein YhfX
RA 3,513,409 C→T S77N (AGC→AAC)  trpS ← tryptophan‑‑tRNA ligase
RA 3,513,818 C→T G191D (GGC→GAC)  gph ← phosphoglycolate phosphatase
RA 3,515,325 G→A H197Y (CAC→TAC)  dam ← DNA adenine methyltransferase
RA 3,518,683 G→A P128S (CCG→TCG)  aroK ← shikimate kinase 1
RA 3,523,302 C→T V144V (GTC→GTT mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,525,188 C→T A773V (GCC→GTC)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,531,645 C→T M265I (ATG→ATA yhgE ← putative transport protein YhgE
RA 3,539,633 G→A G750D (GGT→GAT)  yhgF → putative RNA‑binding protein YhgF
RA 3,539,686 G→A A768T (GCA→ACA)  yhgF → putative RNA‑binding protein YhgF
RA 3,540,703 G→A E99E (GAG→GAA feoB → Fe(2(+)) transporter FeoB
RA 3,541,225 G→A A273A (GCG→GCA feoB → Fe(2(+)) transporter FeoB
RA 3,542,695 C→T C763C (TGC→TGT feoB → Fe(2(+)) transporter FeoB
RA 3,546,362 C→T intergenic (+163/‑197) nfuA → / → gntT iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
RA 3,553,272 C→T S63L (TCG→TTG)  malT → DNA‑binding transcriptional activator MalT
RA 3,557,528 C→T L184L (CTG→CTA rtcB ← RNA‑splicing ligase
RA 3,559,945 C→T intergenic (+79/+677) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,566,536 C→T A16T (GCT→ACT)  glgP ← glycogen phosphorylase
RA 3,571,615 C→T V630M (GTG→ATG)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,586,056 C→T Q256Q (CAG→CAA ggt ← glutathione hydrolase proenzyme
RA 3,611,604 C→T V44I (GTC→ATC)  yhhS ← putative transporter YhhS
RA 3,639,771 C→T intergenic (‑51/‑340) uspB ← / → uspA universal stress protein B/universal stress protein A
RA 3,640,380 C→T D90D (GAC→GAT uspA → universal stress protein A
RA 3,642,547 G→A P196S (CCG→TCG)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase
RA 3,643,308 G→A F625F (TTC→TTT prlC ← oligopeptidase A
RA 3,649,441 C→T G169G (GGC→GGT arsB → arsenite/antimonite:H(+) antiporter
RA 3,650,642 G→A A136T (GCG→ACG)  arsC → arsenate reductase
MC JC 3,653,231 Δ52,001 bp IS5‑mediated yhiS[dppB] 43 genes
RA 3,705,623 G→A L56L (CTC→CTT dppB ← dipeptide ABC transporter membrane subunit DppB
RA 3,706,152 G→A G518G (GGC→GGT dppA ← dipeptide ABC transporter periplasmic binding protein
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
MC JC 3,708,467 Δ168 bp [proK] [proK]
RA 3,708,813 G→A P555S (CCG→TCG)  eptB ← phosphoethanolamine transferase EptB
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,775,395 G→A L324L (CTG→CTA mtlD → mannitol‑1‑phosphate 5‑dehydrogenase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,824,443 G→A A682T (GCG→ACG)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,831,443 C→T S329S (AGC→AGT yicH → AsmA family protein YicH
RA 3,858,618 G→A P500L (CCG→CTG)  yidK ← putative transporter YidK
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,124 C→T V666M (GTG→ATG)  gyrB ← DNA gyrase subunit B
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,938,295 +T coding (69/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,966,690 C→T R92C (CGC→TGC)  rho → transcription termination factor Rho
RA 3,991,149 C→T intergenic (‑383/‑4) hemC ← / → cyaA hydroxymethylbilane synthase/adenylate cyclase
JC 3,991,164 +CTAT coding (12/2547 nt) cyaA → adenylate cyclase
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,005,873 G→A A4T (GCG→ACG)  recQ → ATP‑dependent DNA helicase RecQ
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,046,937 C→T intergenic (‑335/‑29) yihG ← / → polA 1‑acylglycerol‑3‑phosphate O‑acyltransferase YihG/DNA polymerase I
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,124,168 C→A G97* (GGA→TGA)  cytR ← DNA‑binding transcriptional repressor CytR
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,159,493 C→T G433D (GGC→GAC)  sthA ← soluble pyridine nucleotide transhydrogenase
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,207,546 G→A intergenic (‑14/‑601) purH ← / → rrsE bifunctional AICAR transformylase/IMP cyclohydrolase/16S ribosomal RNA
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,226,518 G→A K897K (AAG→AAA metH → cobalamin‑dependent methionine synthase
RA 4,240,006 G→A A17V (GCT→GTT)  yjbT ← PF17089 family protein YjbT
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,341,309 G→A P107L (CCG→CTG)  melR ← DNA‑binding transcriptional dual regulator MelR
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,610,304 +C coding (891/1590 nt) prfC → peptide chain release factor RF3
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD
JC JC 4,640,418 IS30 (–) +2 bp coding (17‑18/141 nt) yjjY → uncharacterized protein YjjY

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196316 1211460 15145 32 [30] [30] 33 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 111 [0] [0] 125 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2726239–2729245 2730813–2729577 333–4575 32 [31] [30] 32 rrlG–[rrsG] rrlG,gltW,[rrsG]
* * ÷ NC_000913 3423811–3424233 3424457–3424239 7–647 32 [31] [30] 33 [rrlD] [rrlD]
* * ÷ NC_000913 4035358–4035733 4036902–4035735 3–1545 33 [31] [31] 32 [rrsA] [rrsA]
* * ÷ NC_000913 4287449 4312942 25494 108 [0] [0] 87 ytcB–[alsR] ytcB,nrfA,nrfB,nrfC,nrfD,nrfE,nrfF,nrfG,gltP,yjcO,fdhF,mdtP,mdtO,mdtN,ytcA,yjcS,alsK,alsE,alsC,alsA,alsB,[alsR]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994981 (0.010)104 (1.200) 66/574 0.1 99.1% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1299499 =NA (NA)98 (1.140) 68/574 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)110 (1.220) 74/598 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)124 (1.380) 83/598 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2288919 =NA (NA)108 (1.200) 80/598 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4287448 0 (0.000)intergenic (‑77/‑163) acs/ytcB acetyl‑CoA synthetase (AMP‑forming)/protein YtcB
* ? NC_000913 = 3653230NA (NA)85 (0.950) 51/598 0.5 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 4312943 = 0 (0.000)coding (49/891 nt) alsR DNA‑binding transcriptional repressor AlsR