Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,622,358	G→A	G42D (GGC→GAC)	purN →	phosphoribosylglycinamide formyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,622,358	0	G	A	100.0%	159.4 / NA	45	G42D (GGC→GAC)	purN	phosphoribosylglycinamide formyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/36);  total (9/36)

CACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCGGTGCATTTCGTCAC  >  NC_000913/2622067‑2622652                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                                                        cactaTGAAATGGATCACATCTTCGACTTCGGCGTTGGGATGACCATTGCCCTGCCTACTACTGAAGTGGACAAAGCCCTCGCACTGCTCAATGTCAACAGTGAAAACGCGGGGAAAAGCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTAGTTATCGAAGAATGAATATTGTGGTACTTATTTCCGGCGACGGAAGTAATGGACAGGCAGTTATTGACGCTTGTAGGATCAGCAAAATTAAAGGCACTGTACGGGCGGGGTGCAGCAATAAGGCCGACGCGTTCGACCTTGAAc                                                                                                                                                                                                                                                                                                                                 <  2:100997/296‑1 (MQ=255) cACCATGAAATGTATCGCACCTTCAACTGCGGCTTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAAc                                                                                                                                                                                                                                                                                                                                 <  2:328401/300‑1 (MQ=255)               tcacACCTCCAATTGCGGCGTCGTGATGATTCTTGCCCTGCCTACTCCGGAAGTGGACAAAGTCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGGGGAAAATCGGTAGCATCAAAGCTTCGGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGTGCAGGTTGCAGCAATAAGGCCGACGCGTTCGACCTTGAATGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCg                                                                                                                                                                                                                                                                                  <  2:278104/297‑1 (MQ=255)               tCGCACNTTCAACTGCGGCGTCTGGATGATTATTTCCCTCCCTGCTCCGGAAGTNGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAAGTATTGACGCCTTTAAAACCAACAAAATTAAAGACACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCg                                                                                                                                                                                                                                                                                  <  2:563199/300‑1 (MQ=255)               tCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCg                                                                                                                                                                                                                                                                                  <  1:180616/300‑1 (MQ=255)                   aCCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCgggcg                                                                                                                                                                                                                                                                              >  1:304858/1‑298 (MQ=255)                    ccTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGTAAGTGGACAAAACCCTCGCCCTGCTTAATGCCAACGGTGAAAACGCTTGGAAAATCGGTATCATCAAAGCCTCTGAGTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTAt                                                                                                                                                                                                                                                                             <  2:193282/300‑1 (MQ=255)                    ccTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCgggcgc                                                                                                                                                                                                                                                                             >  2:85944/1‑297 (MQ=255)                         aaCTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTGATCGAATAATGAANATTGTGGTGCTTATTTCCGGCAACGGNAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCaa                                                                                                                                                                                                                                                                        <  2:471534/300‑1 (MQ=255)                                         tgatTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCACTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGACTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTCCGAACTTGAATGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCgggcgcgtttaaccttgaacgcacccg                                                                                                                                                                                                                                                        >  2:26767/1‑276 (MQ=255)                                            ttattGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACNGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTNCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCgggcgctctcaaacttgaacgcgcccgcca                                                                                                                                                                                                                                                     >  2:641153/1‑273 (MQ=255)                                            ttattGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCGACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCANCAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCgggcgcgttcgaacctgcacgcgccagcca                                                                                                                                                                                                                                                     >  2:603420/1‑273 (MQ=255)                                            ttattGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACCGTGAAAACGCGTGGAAAATCGGTATCATCAAAACCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCTGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGAGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGtgcggccgaacttgaacgcacccgacg                                                                                                                                                                                                                                                     >  2:300220/1‑274 (MQ=255)                                              attGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGTTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTAAAGGCAATTATTGACGCCTGTAAAACCAACAAAAGTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAgcg                                                                                                                                                                                                                                                   <  1:611402/300‑1 (MQ=255)                                                                gAAGTGGACAAAGCTCTCACCCTGTTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGGACAACGCGTGGTTATCAAATAATGAATATTTTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGTAGTTTTCAGCAGTAAGGCCGGCGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAg                                                                                                                                                                                                                                 <  2:76585/300‑1 (MQ=255)                                                                                                       gAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACATGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACg                                                                                                                                                                                          <  1:648582/300‑1 (MQ=255)                                                                                                                tGGAAANTCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAANAATGAATATGGGGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTTTAAAACCAACAAAATTAAATGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGATTTCATTCATGAAATCGACATGTACGCACCCGAtg                                                                                                                                                                                 <  2:576985/300‑1 (MQ=255)                                                                                                                                        tctGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTNCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTNGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATgc                                                                                                                                                         <  2:641810/300‑1 (MQ=255)                                                                                                                                                    cgacGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGcc                                                                                                                                             <  2:325374/298‑1 (MQ=255)                                                                                                                                                               tATCGAATAATGAAGATGGTGGTGCTTATTTCCGTCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGtc                                                                                                                                  <  2:533933/300‑1 (MQ=255)                                                                                                                                                                     ataatGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAGAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCCAGGCGGGCGCGTTCGACCTTGAACGCGCCCGCAAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGTTGGCTGGtttt                                                                                                                                                             >  2:399928/1‑300 (MQ=255)                                                                                                                                                                      taaggAATATTGTGGTGCTTATTTCCGGCAACGGAAGGAATTGACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGTTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACt                                                                                                                           <  1:214834/296‑1 (MQ=255)                                                                                                                                                                          gAATATTGTGGTGCTTATTTCCGGCAGCGGAAGTAATTCACAGTCAAGTATTGACGCCTGTAAAACCAACAAAATTAAATACACCGTACGGGCAGGTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACACTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGTTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGc                                                                                                                       <  2:134532/300‑1 (MQ=255)                                                                                                                                                                          gAATATTGTGGTGCTTATTTCCGACAACGGAAGTAATTTACATGCAATTATTGACGCCTGTAAAGCCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGTGTNTTGCAACGCATACGCTCATCGCCAGCNCGTTTGACAGTCGTGAAGCCTATGACCGGTAGTTGATTCATGAAATCGACATGTACGCACTCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGc                                                                                                                       <  2:475046/300‑1 (MQ=255)                                                                                                                                                                                            ttCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACAt                                                                                                     <  1:17305/300‑1 (MQ=255)                                                                                                                                                                                                 acAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCTTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCTAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCAcc                                                                                                <  2:271302/299‑1 (MQ=255)                                                                                                                                                                                                          gTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTctgc                                                                                       <  1:558958/300‑1 (MQ=255)                                                                                                                                                                                                          gTAATTTACAGGAAGGTATTGACGCCTGGAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTGTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCACCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACACTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCGCTATGCCGGGCGTTTGCTGAACATTCACCCTTCTctgc                                                                                       <  2:80147/300‑1 (MQ=255)                                                                                                                                                                                                                    ggCAATTATTAACTCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAata                                                                             <  2:89546/300‑1 (MQ=255)                                                                                                                                                                                                                                 gCCTGTAAAACCAACAAAATTAAAGACACCGTACGGGCAGTTTTCAGCAATAATGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTacac                                                                <  1:224453/300‑1 (MQ=255)                                                                                                                                                                                                                                   cggTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGGTTTCAGCAAGAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGNAGTTGATTCATGAAATCGACATGTACNCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGACTacacac                                                              <  2:459795/298‑1 (MQ=255)                                                                                                                                                                                                                                   cTGTGAAACCAACAGAACTAAATGCACCGTACGTGCAGTTCTCAGCAATCAGGCCTACGCGTTCGACCTTGAACGCGTCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCAGGAAATCGACATGTACGCACCCGATGTAGTCGTGCTGGCTGGTTTTATGCGCACTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCGAAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGACTacacac                                                              <  2:110642/300‑1 (MQ=255)                                                                                                                                                                                                                                   cTGTAAAACCAACAAAATTAAAGGCACCTTACGTGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTacacac                                                              <  2:668166/300‑1 (MQ=255)                                                                                                                                                                                                                                    tggaaaaCCAACAAAATGAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCGCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTTACCCTTCTCTGCTGCCGAAATATCCCGGATTACACAcc                                                             <  2:134924/297‑1 (MQ=255)                                                                                                                                                                                                                                    tGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACAcc                                                             <  1:152561/300‑1 (MQ=255)                                                                                                                                                                                                                                           ccAACAAAATGAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTc                                                      <  2:382672/300‑1 (MQ=255)                                                                                                                                                                                                                                                           cACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATgg                                      <  1:18852/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                cAATAAGGCCGACGCGTTCGATCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGGTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTGTGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATc                 <  2:391991/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                cAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATc                 <  2:397775/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aTAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGTGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCTAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCgg               <  1:26816/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aTAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCgg               <  1:333802/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aTAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCCGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTGGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCgg               <  2:584952/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ggcgggcgcgTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGACGCCTATGACCGGGAGTTGATTCATGAAATCGAAATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTACCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCGGTGCa           >  1:195249/7‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ggcgggcgcgTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCAGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGCTTACACACCCACCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCGGTGCa           >  1:192781/7‑300 (MQ=255)                                                                                                                                                                                                                                                                                               gTTCGACCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCGGTGCATTTCGTCAc  <  2:30547/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                                                        CACCATGAAATGTATCGCACCTTCAACTGCGGCGTCGGGATGATTATTGCCCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTACACACCCATCGTCAGGCGCTGGAAAATGGCGATGAAGAGCACGGTACATCGGTGCATTTCGTCAC  >  NC_000913/2622067‑2622652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑