Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	186,539	C→T	T704T (ACG→ACA)	glnD ←	protein‑PII uridylyltransferase/uridylyl‑removing enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	186,539	0	C	T	96.2%	167.0 / NA	52	T704T (ACG→ACA)	glnD	protein‑PII uridylyltransferase/uridylyl‑removing enzyme
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (22/28);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTTTCAACAGTAAAATGGCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTATTCC  >  NC_000913/186244‑186834                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        cGGTTTCAACAGTAAAATGGCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAg                                                                                                                                                                                                                                                                                                     <  1:667987/300‑1 (MQ=255)   gTTTCAACAGTAAAATGGCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGcc                                                                                                                                                                                                                                                                                                   <  1:28111/300‑1 (MQ=255)          cAGTAAAATGGCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGc                                                                                                                                                                                                                                                                                            >  1:530819/1‑300 (MQ=255)               aaaTTGCGGAATTCGGCGGGTTGGCGACGGGGCGGCGGTGGCTGCCGGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATTACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGTGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGc                                                                                                                                                                                                                                                                                       <  2:88339/300‑1 (MQ=255)               aaaTGGCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGTGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGc                                                                                                                                                                                                                                                                                       <  1:246414/300‑1 (MQ=255)                    gCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATAc                                                                                                                                                                                                                                                                                  <  1:679440/300‑1 (MQ=255)                                      gCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATc                                                                                                                                                                                                                                                                <  1:272829/300‑1 (MQ=255)                                      gCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCGCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTaagtc                                                                                                                                                                                                                                                                <  1:285758/300‑4 (MQ=255)                                                 gCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTACACGTGTAGCCTGCGGGCTAAGCAATCACAGCGGTTTGCTTAAATCATGTTGtaata                                                                                                                                                                                                                                                     >  2:369498/1‑300 (MQ=255)                                                      ggCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATgg                                                                                                                                                                                                                                                <  1:631763/300‑1 (MQ=255)                                                               gaagtttGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGCCGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTAGGTGCCTACAAGCGTAGCATGCGAGCTAAGCAATACCAGTGATTTGCTCAAATCATACGGTAATAAATGGCGGGCATga                                                                                                                                                                                                                                       >  2:240057/5‑299 (MQ=255)                                                                     tGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATgccaggcc                                                                                                                                                                                                                                 <  1:583252/300‑1 (MQ=255)                                                                          cAGTACTTGCTCCAGACCAAACCGAATAACCTCATGGCGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGTTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAAATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTg                                                                                                                                                                                                                            <  2:15207/300‑1 (MQ=255)                                                                               cgtgcgccAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACTATAAAGTTATCCATCGCCATACCTTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACGCTTAAATTGCGGCGGTCTAATCCGGCACAGACGGCGGCAAACAGATAGGGGCGTTCCGTGCTCCAGATAAAAATCTCGGTGCCTCCACGTTTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTg                                                                                                                                                                                                                       <  2:130823/294‑1 (MQ=255)                                                                                  gCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGc                                                                                                                                                                                                                    <  2:670106/300‑1 (MQ=255)                                                                                   cTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCt                                                                                                                                                                                                                   >  1:296018/1‑300 (MQ=255)                                                                                        gACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGc                                                                                                                                                                                                              <  1:286298/300‑1 (MQ=255)                                                                                            aaaCCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTAGGTGCCTACACGTGTAGCCTGCGGGCTGAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGAc                                                                                                                                                                                                          >  2:351707/1‑300 (MQ=255)                                                                                                 gcaTAACCTCATGCCGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACAATAAAGGTATCCATCGCCATACCGTCGCGAGCGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCCGATAAAACTCTCGGTGCCTACAAGTGTAGCATGCGGGCTAAGCAATAACAGTGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAAGCCGGTTGATTTGGGCTGTGGCGGACAAAAt                                                                                                                                                                                                     >  2:147570/3‑300 (MQ=255)                                                                                                      aCCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTACAAGCGTAGCATGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCAAGGCAAGTTGATTTGGGCTATGGCGGACAAAATAGTTa                                                                                                                                                                                                >  2:340660/1‑300 (MQ=255)                                                                                                             gACGATCTGCGGACAGCGGGTTGCCAGCGGGTTCCAGCACGATAAAGGTATCCANCGCCGTACCGGCGCGAGTGGGGAAAATCTGTGCGTCGTGAACACTGAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCAGTCCGGGCTCCAGAGAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGAc                                                                                                                                                                                         <  2:509912/300‑1 (MQ=255)                                                                                                                gATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGc                                                                                                                                                                                      >  2:45613/1‑300 (MQ=255)                                                                                                                          cAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTACACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAAt                                                                                                                                                                            >  2:534662/1‑300 (MQ=255)                                                                                                                                            ttCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTACAAGTGTAGCATGCGGGCTAAGCAATACCAGCGGTTTGCGTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTATtc                                                                                                                                                          >  2:246484/1‑300 (MQ=255)                                                                                                                                              ccAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTtcgt                                                                                                                                                        <  1:682523/300‑1 (MQ=255)                                                                                                                                               cAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTATAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTtcgtc                                                                                                                                                       <  1:689947/300‑1 (MQ=255)                                                                                                                                                    cGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGt                                                                                                                                                  >  1:387948/1‑300 (MQ=255)                                                                                                                                                      aTAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTACACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGTTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGATATGGCGGACAAACTCGTTAGCACGAAAGCATGACCAAATTTGGTGCAGCGCCTCTtagtcgccgtta                                                                                                                                                >  2:195479/1‑294 (MQ=255)                                                                                                                                                         aaGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATcc                                                                                                                                             <  1:624411/300‑1 (MQ=255)                                                                                                                                                               tCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATgcgc                                                                                                                                       <  1:580664/300‑1 (MQ=255)                                                                                                                                                                   tCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCagta                                                                                                                                   >  2:523783/1‑300 (MQ=255)                                                                                                                                                                   tCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTACACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTATTCGTCGATGTTATCCATGCGCagta                                                                                                                                   >  2:371556/1‑300 (MQ=255)                                                                                                                                                                          aCCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTagtgccag                                                                                                                            <  1:511764/300‑1 (MQ=255)                                                                                                                                                                             gTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTagtgccagtgc                                                                                                                         >  1:580337/1‑300 (MQ=255)                                                                                                                                                                                         gAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTg                                                                                                             <  1:627640/300‑1 (MQ=255)                                                                                                                                                                                         gAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGGCTAATTCGGCACAGAAGGCGGCAAACAGATAAGGGCGGTCCGGGCTCAAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTg                                                                                                             <  1:627912/300‑1 (MQ=255)                                                                                                                                                                                                     gtcgtGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCCGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAAcctg                                                                                                 >  2:316754/1‑298 (MQ=255)                                                                                                                                                                                                                      tGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCg                                                                                <  1:261345/300‑1 (MQ=255)                                                                                                                                                                                                                             gTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTg                                                                         >  2:655841/1‑300 (MQ=255)                                                                                                                                                                                                                                tAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCAGGCGTGTTTTGCAt                                                                      <  1:373594/300‑1 (MQ=255)                                                                                                                                                                                                                                      ggCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCATCTTAGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCATGGAGTTGGTGATGGCGAACCCGTTTGCGCATGTTCGGCGTGTTTTGCATTCcgcg                                                                >  2:72949/1‑300 (MQ=255)                                                                                                                                                                                                                                          caGACGGCTGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACTTGTAGCCTGCGGGCGAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGcgtcgt                                                            <  1:48680/300‑1 (MQ=255)                                                                                                                                                                                                                                          caGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGcgtcgt                                                            <  1:48068/300‑1 (MQ=255)                                                                                                                                                                                                                                                          gATAAGGGCGGTCCGGGCTCCAGATAAAACTCTCGGTGCCTCCACGTGTCGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAACTGGCGGGCATGCCAGGCAAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTgg                                            >  1:234611/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGTCTACAAGTGTAGCATGCGGGCTAGGCAATACCAGTGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGACCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTAGGTgg                                            >  2:251215/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCNGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCNGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTgg                                            >  2:642401/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              cAGATAAAAATCTCGGTGCCGCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGCGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAAc                        <  1:131266/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aaaaaTCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAACAGACt                   <  1:370884/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGc        <  1:307690/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 cTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGAAAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTAt     >  2:3605/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 cTCCACGTGTAGCCTGCGGGCTAAGCAATACCAACGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGAACAAATTTGGTGCAGCGCCTCTTAGTCGATGTTATCCATGNGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCAGTGGTAAAGTAGCGCTCACGCAACAGAATTTGCTTACAGCTAt     >  2:163403/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    cACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATTATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTATTcc  >  2:229905/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        CGGTTTCAACAGTAAAATGGCGTAATTTGGCGGGTTGGCGACGGGGCTGCGGTGGCTGCCAGCTACTTTGCGTCAGTACTTGCTCCAGACCAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCCGCGTCGTAGCTGCTTTTCGGTGGCAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTATTCC  >  NC_000913/186244‑186834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑