| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 441,092 | G→A | P4S (CCG→TCG) | ispA ← | geranyl diphosphate/farnesyl diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 441,092 | 0 | G | A | 98.8% | 300.7 / ‑6.7 | 85 | P4S (CCG→TCG) | ispA | geranyl diphosphate/farnesyl diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (50/34); minor base C (1/0); total (51/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGTTGGCAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGT > NC_000913/440800‑441384 | cAGGTTGGGAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGGGTAAGCGTGGATACACTCAACGTCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCATGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTcc < 2:221067/300‑1 (MQ=255) gTTGGCAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGAACGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCAtt > 1:128540/1‑300 (MQ=255) tGGCAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTAc < 1:697094/300‑1 (MQ=255) tGGCAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCACTAc < 2:675910/300‑1 (MQ=255) tGGCAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGACTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTAc < 2:41374/300‑1 (MQ=255) aaaCCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCAt < 1:316066/300‑1 (MQ=255) gcgACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCNGGCGCTTACCACCTAATAATGCGCCANACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTcc < 2:452758/300‑1 (MQ=255) cAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTa > 1:613038/1‑300 (MQ=255) gATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTaaa > 1:300467/1‑300 (MQ=255) tCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAg < 1:34471/300‑1 (MQ=255) gTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAggg < 1:386438/300‑1 (MQ=255) gTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGAACACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCATGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCCTTGTCCGGTGTAAAAggg > 2:342536/1‑300 (MQ=255) atcCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAANGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTNTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTaga > 2:540683/1‑300 (MQ=255) atcCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTaga > 1:284633/1‑300 (MQ=255) atcCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCATGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAGAAGGGGTTaga > 1:175358/1‑300 (MQ=255) tcCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTGGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTagag < 1:317988/300‑1 (MQ=255) tcCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATTCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTagag < 1:79721/300‑1 (MQ=255) tcCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTagag < 1:164107/300‑1 (MQ=255) aTTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAgg < 1:264481/300‑1 (MQ=255) cGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCt > 2:195303/1‑300 (MQ=255) tcatcaTGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGt > 1:206345/1‑300 (MQ=255) aGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTAcg > 1:572747/1‑300 (MQ=255) gCGTGGATACACTCCACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGCACCTATGACCGGTGGCATAAACCAGGAAGGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGAACACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTaagc > 1:165217/1‑297 (MQ=255) gCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTAcgc > 1:522150/1‑300 (MQ=255) gCGTGGATACACTCAACGGCGGCAGCGAGTGCGTCAAGCGTGTTTGTGCTAACGNCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGTTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTNCGGTGTAAAATCGGTTAGATAGGCGTCTTCATTGTCAGACAGCAGAATTTGTAcgc < 2:641713/300‑1 (MQ=255) tGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTACGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGAAAGCAGAATTTGTACGCGCt > 1:569219/1‑300 (MQ=255) acTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGNGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGc < 2:611291/300‑1 (MQ=255) acTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGc < 1:555013/300‑1 (MQ=255) acTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTAGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGc < 1:40722/300‑1 (MQ=255) cTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACCGCAGAATTTGTACGCGCTGTTTGGCt > 2:132579/1‑300 (MQ=255) cTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCt > 1:192656/1‑300 (MQ=255) cTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTAACAGGCGCTTACCACCTAACAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTCCTGAAAGGGCAGTGGGGCGATCAAGCGGTTCAGCGCATGGTTGACCTGCTTAACGCAGGCCTCGAGTTGCTGCGAAGAGTCCATTACTCATTGTCCGGTGTAGAAGGGGTTAGAGAGGAGTCTTCATTGCCAGACAGCAGAATTCATACGCGCTGTTCGGCt > 2:135933/1‑300 (MQ=255) tCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCtt < 1:591245/300‑1 (MQ=255) cggcAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTaa > 2:14929/1‑300 (MQ=255) gcAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAAtt < 1:160313/300‑1 (MQ=255) gggTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGcc < 2:205281/300‑1 (MQ=255) cAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAGGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGa > 2:531100/1‑300 (MQ=255) aGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTTGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGAc < 1:274231/300‑1 (MQ=255) aGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTNGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGNTCGGCTTGTTGTAATTTGGCCTGCCCCTGAc < 2:476498/300‑1 (MQ=255) aGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGAc < 2:449202/300‑1 (MQ=255) gCGTGTNTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCNCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACg < 2:564551/300‑1 (MQ=255) tgCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACNCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTg > 1:501596/1‑300 (MQ=255) gCCGAACATATGACCGTTGGCATAAACCAGGAAAGGTCGCAGGCGCCTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAATGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCAcgccg < 2:302775/300‑1 (MQ=255) aCATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTc > 1:251171/1‑300 (MQ=255) cATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCg > 1:70113/1‑300 (MQ=255) cATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGNTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCg > 2:518146/1‑300 (MQ=255) ggTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTc < 1:181995/300‑1 (MQ=255) ggCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑CTCAgc > 1:247098/1‑300 (MQ=255) aaCCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCctct > 1:432402/1‑300 (MQ=255) aaCCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCCGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCACGTTCGACCTCG‑TTCAGCGCctat > 2:100098/1‑298 (MQ=255) aaGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCgg < 1:409407/300‑1 (MQ=255) cgcTTACCACCTAATAACGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCATGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTATGCGTTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGACGCGTTCGAACTCG‑TTCAGCGCCTCTTACAGCGGCAGGTCGCCAc > 2:55588/1‑300 (MQ=255) cTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACtt > 1:611366/1‑300 (MQ=255) accTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGGAGTGGGGCGACAAAACAGCTCAGCGCCTGGTTGGCCTGCTTAAAGCAGGCTTCGAGTTTCTGCAAAAAGTCCATTCCCTATTTTCCGGTGTAAAAGGGGTTCAAGAGGAGTCTTCCTTTTCAGACAGCCGAATTTGTCCGCGCTGTTCGGCTTGGTGTAATTTGGCCTGCCCCTGACATGCCAGCTGCCCTCCACGTTCGAAC‑CGATTCAGCTCCTCTTCCAGCGGAAGGTCGcccatgtccaga > 2:587354/1‑290 (MQ=255) gcgcCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCACCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACaa > 2:420190/1‑300 (MQ=255) tccATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATc < 2:398691/299‑1 (MQ=255) gcCATGCTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCTAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATc < 2:10025/300‑1 (MQ=255) ccATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCt > 1:172157/1‑300 (MQ=255) aTACTGCATGGTTTCGACCACGGGAGTGTTCTGCAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATccgt > 2:629658/1‑297 (MQ=255) aTACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCCGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGt > 2:368994/1‑300 (MQ=255) aTACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGt > 2:122619/1‑300 (MQ=255) gCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTNTAAAAGGGGTTAGAGAGGCGTCTTCANTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAg > 2:470370/1‑300 (MQ=255) gCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAg > 1:104699/1‑300 (MQ=255) atgttttCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGAGAAAACGGCTCAGCGCCTGTTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAgct < 1:584745/296‑1 (MQ=255) aTGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAgct < 1:354546/300‑1 (MQ=255) gACCACGGGAGTGTTCTGAAAGGGCAGTGGGNCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGNTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTCTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAgc > 2:493197/1‑300 (MQ=255) ggAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCNTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCtttt > 1:492039/1‑300 (MQ=255) ggAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCtttt > 2:678639/1‑300 (MQ=255) gtTCTGAAAGGGCAGTGGGTCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCACCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAg > 2:118160/1‑300 (MQ=255) gtTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAg > 1:52762/1‑300 (MQ=255) aGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGGTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTTTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCTGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTggcggg < 2:239416/300‑1 (MQ=255) gCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGgcgc > 2:378473/1‑300 (MQ=255) cGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCAttttt > 1:624173/1‑300 (MQ=255) aaaaCGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTttcttc < 2:359041/300‑1 (MQ=255) gCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATa > 2:529206/1‑300 (MQ=255) gCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATa > 1:352864/1‑300 (MQ=255) gcgcCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGCAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATaatgaa > 2:579082/1‑300 (MQ=255) gcgcCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGAAGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATaatgaa > 2:579077/1‑300 (MQ=255) ggCCTGCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGActc > 2:413562/1‑300 (MQ=255) gCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAat > 1:32098/1‑300 (MQ=255) gCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCAAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAat > 2:283640/1‑300 (MQ=255) gCTTAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTCCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCAACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAat > 2:98054/1‑300 (MQ=255) ttAACGCAGGCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCNGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAatat < 1:462884/300‑1 (MQ=255) ggCTTCGAGTTGCTGCGAAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGcc > 1:293853/1‑300 (MQ=255) tgctgcGAAAAGTCCACTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGt < 1:642635/300‑1 (MQ=255) | CAGGTTGGCAAACCGCGACGCAGATCGTCATCATCCATTGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTACTCATTGTCCGGTGTAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCG‑TTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTCGCTCAGCGCCTTTTCAAAGCTGGCGGGCGCCTCATTTTTCTTCGGCATAATGAATGTCTGACTCTCAATATTTTTCGCCCCGTCATGGT > NC_000913/440800‑441384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |