Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	460,576	G→A	K563K (AAG→AAA)	lon →	Lon protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	460,576	0	G	A	98.6%	233.3 / NA	70	K563K (AAG→AAA)	lon	Lon protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (33/36);  minor base C (0/1);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAGTCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTG  >  NC_000913/460280‑460856                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                         gACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGNGAAATCTACAGACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGANAAATCa                                                                                                                                                                                                                                                                                       >  2:571453/1‑300 (MQ=255)        tGTTTTTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGTTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCTAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAACCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGAGAAATCATTAAAAc                                                                                                                                                                                                                                                                                <  2:216325/300‑1 (MQ=255)        tGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAAc                                                                                                                                                                                                                                                                                <  2:212954/300‑1 (MQ=255)         gTTTGTCGCGACGTCAAACTCCATGAACGTTCCGGCGCCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGTTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACa                                                                                                                                                                                                                                                                               <  2:25609/300‑1 (MQ=255)                   aCGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAAtt                                                                                                                                                                                                                                                                     >  1:212723/1‑300 (MQ=255)                                tGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCTAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTTAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCAATAAcc                                                                                                                                                                                                                                                        <  2:259492/300‑1 (MQ=255)                                 gAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCt                                                                                                                                                                                                                                                       >  1:190877/1‑300 (MQ=255)                                  aaCATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTg                                                                                                                                                                                                                                                      <  1:438975/300‑1 (MQ=255)                                        cctgCACCGTTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGAc                                                                                                                                                                                                                                                <  1:76139/297‑1 (MQ=255)                                        ccGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGAc                                                                                                                                                                                                                                                <  1:650257/300‑1 (MQ=255)                                        ccGGCACCGCTGCTGGATCGTAGGGAAGTGATTCGCCTCTCCGTGTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATACACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCACGAc                                                                                                                                                                                                                                                <  2:306297/300‑1 (MQ=255)                                             aCCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTAtct                                                                                                                                                                                                                                           <  2:110007/300‑1 (MQ=255)                                                gctgctGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCgg                                                                                                                                                                                                                                        <  1:577796/300‑1 (MQ=255)                                                 ctgctgGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGgt                                                                                                                                                                                                                                       >  2:417387/1‑300 (MQ=255)                                                           gTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGtt                                                                                                                                                                                                                             >  1:566773/1‑300 (MQ=255)                                                                      aTTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTTCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGGCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGt                                                                                                                                                                                                                  <  1:143122/300‑1 (MQ=255)                                                                      aTTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGt                                                                                                                                                                                                                  <  1:169730/300‑1 (MQ=255)                                                                            ctctccttttATACCGAAGATGAAAAGCTGAATGTCGCCCACCTTCATCTGCTGCCGAAGCAGAGTGAACGGAAGGCACTGAAAAAAGGTGAGCTGAACGTCGACGGTAGCGCCATTATCGGAATTAGGCGTTACTACACCTGTGAGGCGGTCATGCGTGGTCTGGAGCGTGAAATCTCCAAACTGGGTCGCAAATCGGTTGAGCAGTTACTGCTCGATAAATCATTAAAACAGAGTGAACTTAACGGCGATAACCTGCAGGACTATCTCGGTGTTCAGCGTTTCGACTATGGTcgcgcg                                                                                                                                                                                                            <  2:521032/292‑1 (MQ=255)                                                                                 cGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGATTATGGTAGAGCGGATaa                                                                                                                                                                                                       >  2:87477/1‑300 (MQ=255)                                                                                  ggTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACTGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTAGCAAAGCGGTTAAGCAATTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTAGGTGTTCAGCGTTTCGACTATGGTAGGGCGGAtggc                                                                                                                                                                                                      >  2:326286/1‑297 (MQ=255)                                                                                                    aaaCTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGGCTATCTAGGTGTTCGGCGTTTCGACTAGGGTAGCGCGGATAACGaaaaaggtgtaggtaag                                                                                                                                                                                    >  2:38275/1‑287 (MQ=255)                                                                                                       cTGAACATCGCCAAACGTCACCTGCTGCCGAAGCGGATTGAACGTAATGCACTGAAAAAAGGTAAGCTTACCGTCGACAATAGCGCCATTATCTGCGTTATTCGTGACTACACCCTTTATGCGGGCGGGCGGGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAACTAACTACGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTa                                                                                                                                                                                 <  2:623673/300‑1 (MQ=255)                                                                                                            cATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCgg                                                                                                                                                                            <  1:368829/300‑1 (MQ=255)                                                                                                              tCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTc                                                                                                                                                                          >  2:59904/1‑300 (MQ=255)                                                                                                               cGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCt                                                                                                                                                                         >  1:447835/1‑300 (MQ=255)                                                                                                                gCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAAACGTGTCGGTAGGGTAACCGGTCTg                                                                                                                                                                        >  2:313107/1‑300 (MQ=255)                                                                                                                   aaaCGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTTACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCAGTCTGGCg                                                                                                                                                                     <  1:277346/300‑1 (MQ=255)                                                                                                                    aaCGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGt                                                                                                                                                                    <  1:29952/300‑1 (MQ=255)                                                                                                                        tCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTAGCGCGGATAACGAAAACAGTGTCGGTCAGGTAACCGGTCTGGCGTGGAc                                                                                                                                                                >  2:31846/1‑300 (MQ=255)                                                                                                                              gctgcCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGt                                                                                                                                                          >  1:550154/1‑300 (MQ=255)                                                                                                                                 gcCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTAGGTGTTCAGCGTTTCGACTATGGTAGCGGGGATGACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTggg                                                                                                                                                       >  2:231670/1‑300 (MQ=255)                                                                                                                                  ccgagtnagagtgaacgtggtgcgttggaaaaaGTTGATCTGACTGTCGACGATNGCGCCATTATAGGCAGGACTCGTTACTACACCCTTTATGCGTGCGTGCGTGGTCTGGAGCGTTAAATACCCAAGCTGTGTCGCAAGTCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGTCGATAACCTGCATGACTAGCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGc                                                                                                                                                      <  2:573000/272‑1 (MQ=255)                                                                                                                                  ccGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGc                                                                                                                                                      <  1:60076/300‑1 (MQ=255)                                                                                                                                          gATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTAGGTGTTCAGCGTTTCGACTATGGTCGGGGGGGTAAAGAAAACCGTGTCGGTGAGGTAGACGGTCTGGCGTGGACGGAAGTGGGCGGTGattt                                                                                                                                              >  2:271493/1‑297 (MQ=255)                                                                                                                                                aCGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCNAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAANCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAAACGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGAc                                                                                                                                        >  2:549077/1‑300 (MQ=255)                                                                                                                                                aCGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGGCTATGGTCGAGTGGATAACGAAAACAGTGTCGGTCAGGTAGACGGTCTGGCGTGGACGGAAGTGGGCGGTGAATTGCTGAc                                                                                                                                        >  2:588229/1‑300 (MQ=255)                                                                                                                                                 cGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCTGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACTGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGAcc                                                                                                                                       <  1:408125/300‑1 (MQ=255)                                                                                                                                                       gCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTAGAGCGGATAACGAAAACCGTGTCGGTCAGGTAAACGGTCTGGCGTGGACGGAAGTGGGCGGTGAATTGTTGACCACTGaa                                                                                                                                 >  2:104524/1‑300 (MQ=255)                                                                                                                                                         aCTGAAAAAAGGCGAGCTGCTCGTCGACGGTAGCGCCATTCTTGTCATTGTTCGTTGATACACCCGTGATGCGGGCGTGCGTGGTCTGTAGCTTGACATCTCCGAACTGTTTCGCAAAGCGGGTAAGCAGTTACTGCTCGATAAATCATTAAAACATAGCGAAATTAACTTCGATAACCTGCATGACTATCTCGGTGTTCAGCGTGTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAAc                                                                                                                               <  2:310207/300‑1 (MQ=255)                                                                                                                                                            gAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGAATATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGNAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGc                                                                                                                            >  2:505182/1‑300 (MQ=255)                                                                                                                                                                            aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTTAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTaaa                                                                                                            <  1:374118/300‑1 (MQ=255)                                                                                                                                                                            aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTaaa                                                                                                            >  1:82652/1‑300 (MQ=255)                                                                                                                                                                               gTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGc                                                                                                         >  1:299361/1‑300 (MQ=255)                                                                                                                                                                                             cATTATCTGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTTGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATAc                                                                                           <  1:122463/300‑1 (MQ=255)                                                                                                                                                                                             cATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATAc                                                                                           <  1:220524/300‑1 (MQ=255)                                                                                                                                                                                              aTTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATAcc                                                                                          <  1:57200/300‑1 (MQ=255)                                                                                                                                                                                               tgatctgCATTATTCGTTACTACACCCTTGAGGTGGGCGTGCGTGGTCTTGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTANAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTANGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGGGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCg                                                                                         <  2:551310/294‑1 (MQ=255)                                                                                                                                                                                                  gcGGCANTATTCGGTACTACACCCGTGAGGCGGGCGTGCTTTGTCTGGGGCGTGNAATCGCCAAACTGTGGCGCAAAGCGGTTAATCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTACCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGtt                                                                                      <  2:574129/299‑1 (MQ=255)                                                                                                                                                                                                               ttACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACNTGCATGACTATCTCGGTGTTCAGCGTNTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGt                                                                         >  2:466250/1‑300 (MQ=255)                                                                                                                                                                                                                 actacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGa                                                                       <  1:451489/300‑1 (MQ=255)                                                                                                                                                                                                                      acCCTTGAGGCGTGCGTGCGTGGTCTGGGGCGTGAAATCTCCAAACTGTGTCGAAAGGCGGTTAAGCAGTGACTGCTCGATAAATCAGTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGNCTATGGTCGCGCGGATAACGAAAACCNTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAg                                                                  <  2:464305/300‑1 (MQ=255)                                                                                                                                                                                                                      acCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAg                                                                  <  1:399857/300‑1 (MQ=255)                                                                                                                                                                                                                       cccGTGAGGCGGGCGTGCGTGGTCTGGAGCGNGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGANTATCTCGGTGTTCAGCGTTTCGACTAGGGTAGCGCGGATAACGAAAACCGTGTAGGTCAGGTAACCGGTATGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTATTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGGTGCAgg                                                                 >  2:477027/1‑300 (MQ=255)                                                                                                                                                                                                                       cacGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAgg                                                                 >  2:375173/3‑300 (MQ=255)                                                                                                                                                                                                                                 gggcgTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCa                                                       <  1:633127/300‑1 (MQ=255)                                                                                                                                                                                                                                  ggcgTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGCCTATGGTCGCGCGGGTAACGAAAACCGTGTCGGTCAGGTAACCGGTATGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCAAAGTGATGCAGGAGTCCATTCAg                                                      >  2:269855/1‑300 (MQ=255)                                                                                                                                                                                                                                    cgttcttgGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCTAAATTAACTTCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAggc                                                    <  2:623091/294‑1 (MQ=255)                                                                                                                                                                                                                                      tgcgtgGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAAAAGTGTAGGTAGGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCACTCAggcgg                                                  >  2:304187/1‑300 (MQ=255)                                                                                                                                                                                                                                       gcgtgGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTAGAGCGGGTAAAGAAAACCGTGTAGGTCAGGTAGCCGGTCTGGCGTGGACGGAAGTGGGTGGTGACTTGCTGACCATTGAAACAGCATGTGTTCAGGGTAAAGGCACACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTAAggcggc                                                 >  2:12084/1‑300 (MQ=255)                                                                                                                                                                                                                                            gTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCCGGCGGCGTTaa                                            >  2:385398/1‑300 (MQ=255)                                                                                                                                                                                                                                               tGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACgg                                         <  1:210146/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCTCCAAACTGTTTCGCAAAGCGGTCAAGCAGTTACTGCTCGATAACTCATTAAAACATATCGAAATTAACATCGATAACCTACATTACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGTTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg                              <  2:362397/300‑1 (MQ=255)                                                                                                                                                                                                                                                          aTCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg                              <  1:635987/300‑1 (MQ=255)                                                                                                                                                                                                                                                                aaaCTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcgcgtgcg                        >  1:188409/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAAGCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGAGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAAACGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAAc      >  2:538276/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTTAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAAc      <  1:253952/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ttAAGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAAcc     <  1:546000/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      aGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGCCTATGGTCGAGAGGATAACGAAAACCGTGTCGGTCGGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGAGATCAAACCTg  >  2:226831/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      aGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTg  >  1:629543/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      aGCAGTTACTGCTCGATAAATCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGAGAGGGTAACGAAAACCGTGTCGGTCAGGTAAACGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCCCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTg  >  2:590483/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                         GACGTGATGTTTGTCGCGACGTCGAACTCCATGAACATTCCGGCACCGCTGCTGGATCGTATGGAAGTGATTCGCCTCTCCGGTTATACCGAAGATGAAAAACTGAACATCGCCAAACGTCACCTGCTGCCGAAGCAGATTGAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTTAAGCAGTTACTGCTCGATAAGTCATTAAAACATATCGAAATTAACGGCGATAACCTGCATGACTATCTCGGTGTTCAGCGTTTCGACTATGGTCGCGCGGATAACGAAAACCGTGTCGGTCAGGTAACCGGTCTGGCGTGGACGGAAGTGGGCGGTGACTTGCTGACCATTGAAACCGCATGTGTTCCGGGTAAAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTG  >  NC_000913/460280‑460856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑