Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,604,840	C→T	S265F (TCC→TTC)	lsrD →	Autoinducer‑2 ABC transporter membrane subunit LsrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,840	0	C	T	100.0%	197.5 / NA	58	S265F (TCC→TTC)	lsrD	Autoinducer‑2 ABC transporter membrane subunit LsrD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/25);  total (33/25)

ATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGC  >  NC_000913/1604551‑1605127                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                aTCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGc                                                                                                                                                                                                                                                                                       <  1:191415/300‑1 (MQ=255)       cTGATATGTCTCCTCTTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGcc                                                                                                                                                                                                                                                                                 <  1:510496/300‑1 (MQ=255)           tatGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAANCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTg                                                                                                                                                                                                                                                                             <  1:473991/300‑1 (MQ=255)              gTCTACTCGTTTGCTGGCTATGGCTGGAGAAAACCCATGCCGCACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCCTGGCTCTTTATAGCGCGATTCCAGTGAACCGTACCTTATGTGCGCTCTATGAAATGACTGGGCTGGCGTCTGCGGTCGCTGCTGTGTTGCTGGTATCGGATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCgg                                                                                                                                                                                                                                                                          <  2:395816/300‑1 (MQ=255)                      gTTTTCTTGCTCTTGCTGCATAAAACCCATGCCGGACGTAATGTTTTTGTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCGGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTttatta                                                                                                                                                                                                                                                                  <  1:244037/300‑1 (MQ=255)                             ggctctggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGGGTCG‑TTTATGATGCACGCCATCAACGAAGTGGTGCTTGGAGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGat                                                                                                                                                                                                                                                           >  2:356334/1‑300 (MQ=255)                                   ggctGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGc                                                                                                                                                                                                                                                     >  1:442780/1‑300 (MQ=255)                                              aCCCATGCCGGACGTAATGTGTTTTTGATTTGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTg                                                                                                                                                                                                                                          >  1:53873/1‑300 (MQ=255)                                                           gTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAg                                                                                                                                                                                                                             <  1:583282/300‑1 (MQ=255)                                                                                              gcgcTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCTTCA‑CTTCTGATGCCCGCCATCACCGCAGTGGTGCTTGGAGGGGACAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATCAGTGGGATATTTGCAACANGGTTTGCACATGGCAGGAGTGCCACATCCGGTGTCCAGCGCCCTTTCNGGTgcg                                                                                                                                                                                          >  2:563089/1‑300 (MQ=255)                                                                                              gcgcTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTAGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTgcg                                                                                                                                                                                          >  2:431289/1‑300 (MQ=255)                                                                                                gcTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCTTTTCCGGTGCGCt                                                                                                                                                                                        >  2:528622/1‑300 (MQ=255)                                                                                                  tttAGAGCGCGATGCCAGTGAATCGTATCGTATTTGCGCTCTATGTCACGACTGGGCTGGCTTCTGCGGTCGCCGCTGTGCTTTTGGTATCGGCTTTTTTTCCAANACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTGATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGTTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTAc                                                                                                                                                                                      <  2:601568/300‑1 (MQ=255)                                                                                                    tataGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACtt                                                                                                                                                                                    <  1:327874/300‑1 (MQ=255)                                                                                                                   gTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTa                                                                                                                                                                     <  1:24767/300‑1 (MQ=255)                                                                                                                      gatgtgtataagagacagcTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGNCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGt                                                                                                                                                                  <  2:598903/283‑1 (MQ=255)                                                                                                                      cacagTACCTTAGGTGCGCTCTAGGTCATGACGGGGCTGGTGTCTGCGGTCGCCGCGGTGCTATTGGTATCGTATTTTTTCTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGGGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGt                                                                                                                                                                  <  2:445950/296‑1 (MQ=255)                                                                                                                      aaCCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGt                                                                                                                                                                  <  1:25290/300‑1 (MQ=255)                                                                                                                           tACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTc                                                                                                                                                             >  1:355360/1‑300 (MQ=255)                                                                                                                           tACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGAGTGG‑TTTATGATGCCCGCAATCAACGAAGTGGTGCTTGGCGGGGCAGATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTc                                                                                                                                                             >  2:522186/1‑300 (MQ=255)                                                                                                                                       gcTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGGGTAG‑TTTCTGATGCCCGCCATCAACGAAGTGGTGCTTGGCGGGGCCGATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGCCAGGAGTGCCAAATCAGGTGTCCAGCGNCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCa                                                                                                                                                 >  2:505428/1‑300 (MQ=255)                                                                                                                                       gcTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCa                                                                                                                                                 >  1:222638/1‑300 (MQ=255)                                                                                                                                        ctctATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTctgtctcttatacacatc                                                                                                                                                >  1:610340/1‑283 (MQ=255)                                                                                                                                        ctctATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCAt                                                                                                                                                >  1:56849/1‑300 (MQ=255)                                                                                                                                          ctATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCAACGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGANATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCg                                                                                                                                              >  1:506600/1‑300 (MQ=255)                                                                                                                                          ctATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTGTGGTTCAGCACGTTCCGATCTCGGTGATTCG‑TTTCTAATGCCCGCCATCACCGAAGTGGTGCTTGGAGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTACGGTGTGCTACTTATCGTCGTTGTCGCAGGTCGTTCCGTTAGCCTGCATCg                                                                                                                                              >  2:436955/1‑300 (MQ=255)                                                                                                                                                   gACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGGTTAG‑CTTCTGATGCCCGCCATCAACGAAGTGGTGCTTGGCGGGGACGATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAAt                                                                                                                                     >  2:351406/1‑300 (MQ=255)                                                                                                                                                              cGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGtggc                                                                                                                          >  1:405003/1‑300 (MQ=255)                                                                                                                                                                 cTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGtggctgg                                                                                                                       >  2:430708/1‑300 (MQ=255)                                                                                                                                                                               gtgCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGGTTGG‑TTTATGATGCAAGAAATTGGGGAGGGGGTGTTTGGGGGGGAGGAGATTTGTGGTGGTTCCGGTTTCGTTATCGGCACCGTCATTGCGGTTTTCTTAGTGGGATATTTGCAAAAAGGTTTGCAAATGGCAGGAGTGTCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCATGCATCACCAGCAAATTAAAGCGTGGCTGGCGCGTCGGGCCAAt                                                                                                         >  2:448806/1‑300 (MQ=255)                                                                                                                                                                               gtgCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCTTCA‑TTTCTGATTCCCGCCATCACCGCAGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCAATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCACTTTATGGTGCGTTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCAAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAAt                                                                                                         >  2:40293/1‑300 (MQ=255)                                                                                                                                                                               gtgCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCTCCT‑TTTCTGATTCCCGCCATCACCGCAGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGTCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCagt                                                                                                         >  2:96276/1‑298 (MQ=255)                                                                                                                                                                               gtgCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTAG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAAt                                                                                                         >  2:410046/1‑300 (MQ=255)                                                                                                                                                                                    gctgGTATCGTGTTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCATGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAAccc                                                                                                    <  1:225099/300‑1 (MQ=255)                                                                                                                                                                                        gTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTg                                                                                                >  1:343361/1‑300 (MQ=255)                                                                                                                                                                                          aTCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTNTCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGcc                                                                                              <  1:496105/300‑1 (MQ=255)                                                                                                                                                                                             gTATTTTGGTTCAGCACGTTCCGATCTCGGTG‑GTCGGTTTATGATGCCCGCAGTTGACGAAGGGGTGCTTGGGGGGGGAGATATTTATGGTGGTTCAGGTTTCGTTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCACTTTCCGGTGCGCTACTTATCGTCGTTGTTGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCACTAACCCATTGCCATa                                                                                           >  2:31180/1‑300 (MQ=255)                                                                                                                                                                                                ttttGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAg                                                                                        <  1:228171/300‑1 (MQ=255)                                                                                                                                                                                                              gTTCCGATCTCGGTGTTTCG‑TTTCTAATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATANTTGCAACAAGGTTTGCAAATGGCAGGNGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGac                                                                          >  2:473728/1‑300 (MQ=255)                                                                                                                                                                                                              gTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGac                                                                          <  1:267970/300‑1 (MQ=255)                                                                                                                                                                                                                        cGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGc                                                                <  1:427101/300‑1 (MQ=255)                                                                                                                                                                                                                                                   tCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAgcgc                                      <  1:671668/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aCCGCCGGGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCACAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAgcgcgc                                    <  2:436616/300‑3 (MQ=255)                                                                                                                                                                                                                                                      ccgccgTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGctct                                   >  2:370710/1‑300 (MQ=255)                                                                                                                                                                                                                                                          cgTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGc                               <  1:548863/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gtggtgCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGACATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTAATGAAACTTCATCGCTTTAAGAAAATCGCCTTACTTCGCGCTCTTGGCa                              >  2:184965/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ctggtgCTTGGCGGGGCCAATATTTACGGTGGTTCCTGTTTCCTTGTCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAGGTGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCGTGCAAGTGCGCTACTTATCGTCATTGTCGTAGGTCGTTCCGTGAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCACGTCGGGCCAATAACCCATTGCCATAAAGAATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACGTAGCGCTCTTGGCa                              <  2:242449/299‑1 (MQ=255)                                                                                                                                                                                                                                                              gtgCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTg                           >  1:286413/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gtgCTTGGCGGGGCAAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCANCGCCCTTTCCGGTGCGCTACTTATCGNCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTg                           >  2:457827/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gtgCTTGGCGGAGCCAATATTTATGGTGGCTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCGGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGTATTg                           >  2:17366/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         ggCCAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATctct                >  1:160567/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           ccAATATTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTAt              >  2:130614/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              atggttATTGTGGTTCCGGTTTCATTATCGGCATCGCCATTGCGGTGTTATTGTTGGGATATTTGCAATGAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTGGGTCGTTCCGTTAGCTTGCATCGCCAGCAAATTAAAGAGTGGCTGGTGCGTCGGGCCAATAACCCATTGCCATAAGGGATATCTTCATGACACTTCATCGCTTTAAGGAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGaa           <  2:41764/296‑1 (MQ=255)                                                                                                                                                                                                                                                                              atatTTATGGTGGTTCCGTTTTCATTATCGGCACCGCCATTGCGGTTTTAGTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGTTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGaa           <  2:402736/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              atatTTATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGaa           <  1:153790/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               tctttATGGTGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGNATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGNTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAAt          >  2:633711/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                attcaTGGTGGTTCCGGTTTCATTATCGGTACCGCCATTGCGGTTTTATTAGTGGGATAGTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGTTCGTGCCGTTAGCCTGCATCGCCAGCAAATGAAAGAGTGGCTGGCTCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGTTCTTTGCATTTCCGCAATCTCTATGAAtg         <  2:231754/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gtggtTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCAGCAATCTCTATGAATGTGCAGGc  >  1:5667/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                ATCTTCCTGATATGTCTCCTCGTTTTCTGGCTCTGGCTGCATAAAACCCATGCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCG‑TTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGCCAATATTTATGGTGGTTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGC  >  NC_000913/1604551‑1605127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑