Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,557,528	C→T	L184L (CTG→CTA)	rtcB ←	RNA‑splicing ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,557,528	0	C	T	98.9%	316.8 / NA	92	L184L (CTG→CTA)	rtcB	RNA‑splicing ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (41/50);  minor base G (0/1);  total (41/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGCAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACG  >  NC_000913/3557232‑3557823                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        tAGTTGTGGTGACAGTTGATCTCTTCCATCGCCGGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCAAGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCAACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTACTGCATCTCTTTTTGCGCCAGATCGATAAAGTAAGCCCCGATGGCGTTAACACTTCCGCGTGAACCGGAGTgtagc                                                                                                                                                                                                                                                                                                      >  1:342932/1‑300 (MQ=255) tAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAANAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGNACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTgtagc                                                                                                                                                                                                                                                                                                      >  2:542126/1‑300 (MQ=255) tAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCAAGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCAGATGGCGTTACCAATTCCGCGTGAACCGGAGTgtagc                                                                                                                                                                                                                                                                                                      >  2:44987/1‑300 (MQ=255)   gttttggcgACAGTTGATCTCTTCCATCGCCAGCGTTCGTGGCTGGCTGACCGTTTTCTGCGTAATGCTTTGCAATGCCGTTACCACGTTTTCCATCATCTCATCGCGTTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCAGAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCGTCTCTGTTTGTGCCAGATCGATAAAGTAAGTCCCGAGGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    <  2:138836/292‑1 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTCCATCGCTAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAANGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTNCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    <  2:545552/300‑1 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTCCATCGCCNGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAANGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    >  2:492287/1‑300 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    >  1:433855/1‑300 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    <  2:654646/300‑1 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATNGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    >  2:513938/1‑300 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTCCATCGACAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGCTCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    >  1:265830/1‑300 (MQ=255)   gTTGTGGTGACAGTTGATCTCTTACATCGCCAGTGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCATTACCAATTCCACGTGAACCGGAGTGTAGCAt                                                                                                                                                                                                                                                                                                    >  2:197196/1‑300 (MQ=255)     tgtgGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGGCCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATaa                                                                                                                                                                                                                                                                                                  <  1:654235/300‑1 (MQ=255)     tgtgGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATaa                                                                                                                                                                                                                                                                                                  <  2:622366/300‑1 (MQ=255)            aCAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCacac                                                                                                                                                                                                                                                                                           <  1:429752/300‑1 (MQ=255)             cAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTANGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCcacccc                                                                                                                                                                                                                                                                                          >  2:516053/1‑297 (MQ=255)             cAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACAcc                                                                                                                                                                                                                                                                                          <  1:370353/300‑1 (MQ=255)             cAGTTGACCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACAcc                                                                                                                                                                                                                                                                                          <  2:239483/300‑1 (MQ=255)                  gATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTc                                                                                                                                                                                                                                                                                     >  1:213403/1‑300 (MQ=255)                   aTCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTcc                                                                                                                                                                                                                                                                                    >  1:67196/1‑300 (MQ=255)                      tctTCCATCTCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGAc                                                                                                                                                                                                                                                                                 <  2:413366/300‑1 (MQ=255)                      tctTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAATCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGAc                                                                                                                                                                                                                                                                                 <  2:373052/300‑1 (MQ=255)                       ctTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCAATAAAGTACGCCAGATCAAGCGACGGCAACGTCTAAAGCGTTTACTGCATCTATCTTTGTGCCAGATCGATAAAGTACGTCCAGATGGCGTTACCAATTCCGCGTGAACTGGAGTGTAGCATAATCCACACCTGGTCCGAcc                                                                                                                                                                                                                                                                                >  2:58579/1‑299 (MQ=255)                                     gTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATNAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTNCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGAtt                                                                                                                                                                                                                                                                  >  2:540912/1‑300 (MQ=255)                                         gtgGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCaa                                                                                                                                                                                                                                                              >  2:383950/1‑300 (MQ=255)                                            gCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATaa                                                                                                                                                                                                                                                           <  2:405071/300‑1 (MQ=255)                                            gCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATaa                                                                                                                                                                                                                                                           >  1:376215/1‑300 (MQ=255)                                            gCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATaa                                                                                                                                                                                                                                                           >  1:354608/1‑300 (MQ=255)                                            gCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTGTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATaa                                                                                                                                                                                                                                                           <  2:204371/300‑1 (MQ=255)                                             cTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATaaa                                                                                                                                                                                                                                                          >  1:588241/1‑300 (MQ=255)                                               gTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGt                                                                                                                                                                                                                                                        >  1:167700/1‑300 (MQ=255)                                                   gACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGtt                                                                                                                                                                                                                                                    <  2:193295/300‑1 (MQ=255)                                                      cGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTAcc                                                                                                                                                                                                                                                 <  1:208407/300‑1 (MQ=255)                                                       gTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCAAGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCANATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATAAAGGCAGATTTCAATAAAGTGGTTACCg                                                                                                                                                                                                                                                >  1:470917/1‑300 (MQ=255)                                                              gCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCa                                                                                                                                                                                                                                         >  1:191092/1‑300 (MQ=255)                                                                ttAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGc                                                                                                                                                                                                                                       <  2:93998/299‑1 (MQ=255)                                                                     gCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTcc                                                                                                                                                                                                                                  >  1:277728/1‑300 (MQ=255)                                                                      ctctGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTccc                                                                                                                                                                                                                                 >  1:180133/1‑300 (MQ=255)                                                                        ctGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAg                                                                                                                                                                                                                               >  1:298225/1‑300 (MQ=255)                                                                        ctGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTccccg                                                                                                                                                                                                                               >  2:84110/1‑298 (MQ=255)                                                                         tGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAgg                                                                                                                                                                                                                              <  1:51031/300‑1 (MQ=255)                                                                                  gTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTAAATTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAg                                                                                                                                                                                                                     <  2:230895/300‑1 (MQ=255)                                                                                   ttACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGt                                                                                                                                                                                                                    <  2:442606/300‑1 (MQ=255)                                                                                          gTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGt                                                                                                                                                                                                             >  1:232580/1‑300 (MQ=255)                                                                                             ttCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTAtt                                                                                                                                                                                                          >  2:337960/1‑300 (MQ=255)                                                                                             ttCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTAtt                                                                                                                                                                                                          >  1:407408/1‑300 (MQ=255)                                                                                                 atcatcGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAgg                                                                                                                                                                                                      <  2:346076/300‑1 (MQ=255)                                                                                                              ggTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGNATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATAtt                                                                                                                                                                                         >  1:500083/1‑300 (MQ=255)                                                                                                              ggTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTAACGGTTCCCAGTGTTCCAAGGTGCTTATAGCTATTGGTATTTAGGAAACGGGGATAtt                                                                                                                                                                                         >  2:210897/1‑300 (MQ=255)                                                                                                               gTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATAttt                                                                                                                                                                                        >  1:529870/1‑300 (MQ=255)                                                                                                                          cAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACNTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAAcc                                                                                                                                                                             <  2:503045/300‑1 (MQ=255)                                                                                                                            aaaaGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTNACCAATTCCGCGTGAACCGGAGTGTANCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCAc                                                                                                                                                                           <  2:466046/300‑1 (MQ=255)                                                                                                                                 cTGCGCCCATGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATa                                                                                                                                                                      <  2:407554/300‑1 (MQ=255)                                                                                                                                   gcgcCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCNGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATNCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAAc                                                                                                                                                                    <  2:539682/300‑1 (MQ=255)                                                                                                                                    cgcCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAAcc                                                                                                                                                                   <  1:596144/300‑1 (MQ=255)                                                                                                                                     gcCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTACCCACTGATAACCg                                                                                                                                                                  >  1:228210/1‑300 (MQ=255)                                                                                                                                                ggCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTc                                                                                                                                                       <  1:341372/300‑1 (MQ=255)                                                                                                                                                          tAATCATCAAAATATTCCGTACCTTCCATAAAGTACGCCAGATCACGTGACGGCAATGTCTCAAGCGTTTCCTGCATCTCTTCTTGTGCCAGATCGATAAAGGAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCTGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCAttga                                                                                                                                             <  2:592700/300‑3 (MQ=255)                                                                                                                                                             tcatcaAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTNGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCa                                                                                                                                          >  1:462883/1‑300 (MQ=255)                                                                                                                                                              catcaAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCAt                                                                                                                                         <  2:37480/300‑1 (MQ=255)                                                                                                                                                              catcaAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCAt                                                                                                                                         >  1:521552/1‑300 (MQ=255)                                                                                                                                                              catcaAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTAAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCAt                                                                                                                                         >  1:521538/1‑300 (MQ=255)                                                                                                                                                                  aaaGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGAc                                                                                                                                     >  2:383234/1‑300 (MQ=255)                                                                                                                                                                       aTTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTaa                                                                                                                                >  1:97585/1‑300 (MQ=255)                                                                                                                                                                        ttCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAAc                                                                                                                               <  1:33622/300‑1 (MQ=255)                                                                                                                                                                        ttCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGGGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAAc                                                                                                                               <  2:676110/300‑1 (MQ=255)                                                                                                                                                                         tCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACa                                                                                                                              <  2:177851/300‑1 (MQ=255)                                                                                                                                                                         tCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACa                                                                                                                              >  1:524988/1‑300 (MQ=255)                                                                                                                                                                          ccGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAg                                                                                                                             <  1:539827/300‑1 (MQ=255)                                                                                                                                                                                        aaGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTATTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAAACGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAg                                                                                                               >  2:49077/1‑300 (MQ=255)                                                                                                                                                                                          gTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGc                                                                                                             <  1:690675/300‑1 (MQ=255)                                                                                                                                                                                                      aCGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATTAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACg                                                                                                 <  2:595145/300‑1 (MQ=255)                                                                                                                                                                                                             tgCAACGCCTCAAGCGTTTCCTGCGTATCGTTTTGTGCCAGATCGAGAAAGTAANTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAAGCCACACCTGGTCCGACGCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCNATTTAGCATCGACGTTAACAGGTGGATTTTCCCATGCACCTTTATCACGTTTACaa                                                                                          <  2:631918/299‑1 (MQ=255)                                                                                                                                                                                                             ggCAACGTCTCACGCTTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATTGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGTAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACaa                                                                                          <  2:212247/300‑1 (MQ=255)                                                                                                                                                                                                             ggCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGCACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACaa                                                                                          <  2:591818/300‑1 (MQ=255)                                                                                                                                                                                                             ggCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCATGCACCTTTATCACGTTTACaa                                                                                          <  1:159733/300‑1 (MQ=255)                                                                                                                                                                                                              gCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAAc                                                                                         <  1:181460/300‑1 (MQ=255)                                                                                                                                                                                                                  cGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCAAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGcc                                                                                     >  2:624700/1‑300 (MQ=255)                                                                                                                                                                                                                    tctcAAGCGTTTCCTGCATTTATTTTTGTGCCAGATCGATAAAATAAGTCCCGATGGCGTTCCCACTTCCGCGTGAACCGGAGTGTAGCATCATCCACACCTGGTCCGACTCATCAAGGCAGATTTCCATACAATGGTTACCTGTTCCCAGCGTTCCCAGGTGTTCATAGCTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGCTACCCGGCTTCCAGCTCAGCCCCTTTAGCATCGACGTTAACAGGTGGATTTTCCAAGGCACCTTTATCACGTTTACAACGGCCAg                                                                                   >  1:526939/1‑300 (MQ=255)                                                                                                                                                                                                                    tctcAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAg                                                                                   >  1:359287/1‑300 (MQ=255)                                                                                                                                                                                                                       cAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTgg                                                                                <  1:134525/300‑1 (MQ=255)                                                                                                                                                                                                                            gTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTATTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCACGCTCAGCCCATTTCGCATCAACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAATAGTACGc                                                                           >  2:98553/1‑300 (MQ=255)                                                                                                                                                                                                                            gTTTCCTACATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGc                                                                           <  2:311300/300‑1 (MQ=255)                                                                                                                                                                                                                             tttCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGNCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGcc                                                                          <  1:470447/300‑1 (MQ=255)                                                                                                                                                                                                                             tttCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACATGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGcc                                                                          <  2:59625/300‑1 (MQ=255)                                                                                                                                                                                                                             tttCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGcc                                                                          <  1:68330/300‑1 (MQ=255)                                                                                                                                                                                                                             tttCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGcc                                                                          <  2:97268/300‑1 (MQ=255)                                                                                                                                                                                                                             tttCCCGCGTCTCTTTTTTTGTCGGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTGCAATAAGGTTGTTACCGGTTCCCAGCGGTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGTGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTGAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGcc                                                                          <  2:187787/300‑1 (MQ=255)                                                                                                                                                                                                                                            tttGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCg                                                           <  2:676545/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTANCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAAt                                                    <  1:501259/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  agtCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGctc                                     <  1:286007/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     cccGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGc                                  >  1:416766/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cGATGGCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCa                                <  1:187520/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gCGTTACCAATTCCGCGTGAACCGGAGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGttt                           >  1:536466/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  ccAATTCCGCGTGAACCTGAGTGGAGCATAATCCACACCTGGTCCGACGCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGGTTATAGTNATTGGTATTCAGGAAACGGGGATAGTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCGTGATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAAGCGCCTGACGCAGCTCTGCCAGGTTTTcaggc                     <  1:462974/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     aGTGTAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAAcg  <  2:429617/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        TAGTTGTGGTGACAGTTGATCTCTTCCATCGCCAGCGTTTGTGGCTGTCTGACCGTTTTCTGCGTAATGCTCTGCAATGCCGTTACCACGTTTTCCATCATCGCATCGCGGTTAAGGCTGGCAAAAAGCTGCGCCCAGGCCACGGCTTTCAGGTAATCATCAAAGTATTCCGTACCTTCCATAAAGTACGCCAGATCACGCGACGGCAACGTCTCAAGCGTTTCCTGCATCTCTTTTTGTGCCAGATCGATAAAGTAAGTCCCGATGGCGTTACCAATTCCGCGTGAACCGGAGTGCAGCATAATCCACACCTGGTCCGACTCATCAAGGCAGATTTCAATAAAGTGGTTACCGGTTCCCAGCGTTCCCAGGTGTTTATAGTTATTGGTATTCAGGAAACGGGGATATTTTTGCGTTAACCACTGATAACCGGCTTCAAGCTCAGCCCATTTAGCATCGACGTTAACAGGTGGATTTTCCCAGGCACCTTTATCACGTTTACAACGGCCAGTGGTACGCCCGTGCGGCACGGCCGTTTCAATCGCCTGACGCAGCTCTGCCAGGTTTTCAGGCAGGTCTTCCGCCGTTAACG  >  NC_000913/3557232‑3557823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑