Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	280,709	C→T	W9* (TGG→TGA)	yagB ←	orphan antitoxin YagB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	280,709	0	C	T	100.0%	178.8 / NA	55	W9* (TGG→TGA)	yagB	orphan antitoxin YagB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

ATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGC  >  NC_000913/280421‑281004                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                         aTTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACATAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGt                                                                                                                                                                                                                                                                                               <  2:592671/300‑1 (MQ=255)             gatgtataCATACCCATGACTGCCCAGCGTATCAGCTTCACCAGTCAGCCCGTTGCGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACATGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGTTTCATCTAGTTGTAGAGTCTGCACCTCACTGAGTGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCCTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTAtt                                                                                                                                                                                                                                                                                   <  2:331338/296‑1 (MQ=255)             gATATATACATACCCATGACTGCCCAGCTTATCGGCTTCACCAGTCAGTCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTGGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGGTGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTAtt                                                                                                                                                                                                                                                                                   <  2:13336/300‑1 (MQ=255)              atatatACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTc                                                                                                                                                                                                                                                                                  >  1:166519/1‑300 (MQ=255)                        acCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTAt                                                                                                                                                                                                                                                                        >  1:16700/1‑300 (MQ=255)                                                            gCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAat                                                                                                                                                                                                                                    >  1:6398/1‑300 (MQ=255)                                                                 ttACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAACATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCAGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGACTATTtg                                                                                                                                                                                                                               >  2:676208/1‑300 (MQ=255)                                                                 ttACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTtg                                                                                                                                                                                                                               >  1:159865/1‑300 (MQ=255)                                                                   acagTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTCTTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTtgcc                                                                                                                                                                                                                             >  1:157279/4‑300 (MQ=255)                                                                           gtgtGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGccgccg                                                                                                                                                                                                                     >  1:87857/1‑300 (MQ=255)                                                                           gtgtGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTAGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTgccgcag                                                                                                                                                                                                                     >  1:408923/1‑298 (MQ=255)                                                                                     gTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAggt                                                                                                                                                                                                           >  1:157994/1‑300 (MQ=255)                                                                                                         gCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAaca                                                                                                                                                                                       >  1:33219/1‑300 (MQ=255)                                                                                                           tCACAGAAGAGCAGCATCAGCTCCCGATGTGCGACACAATGCGAGAAGGCTTCATCCAGTCGTAGAGTCTGCACCTCACCGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGCTGACCTTCCTGCACCCGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGCAGACCACGTTCGGCGAACGAGAATCTTTGCGTGCAGCCGACAACCAAGTGGTCGGGAACATAGTGAACACt                                                                                                                                                                                     >  2:587842/1‑300 (MQ=255)                                                                                                              caGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTAGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTTATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCATGCCGCCGACAATCAGGTGGTCGGGGACATGGTGCACACtttt                                                                                                                                                                                  >  2:31461/1‑300 (MQ=255)                                                                                                                ggAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTa                                                                                                                                                                                >  1:537327/1‑300 (MQ=255)                                                                                                                  agagCAGCATCAGCTCCAGATGTTCGACAAAATGCTGGAATGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAAc                                                                                                                                                                              <  2:341050/300‑1 (MQ=255)                                                                                                                             aGCTCCAGATGTGCGACAAAATGCTGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTAccc                                                                                                                                                                   <  2:85198/300‑1 (MQ=255)                                                                                                                             aGCTCCAGATGTGCGACAAAATGCGGGAATGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAACCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTAccc                                                                                                                                                                   <  2:16847/300‑1 (MQ=255)                                                                                                                             aGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATTCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTTTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTAccc                                                                                                                                                                   <  2:59849/300‑1 (MQ=255)                                                                                                                             aGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATNACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTAccc                                                                                                                                                                   <  1:485471/300‑1 (MQ=255)                                                                                                                                                                     gTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAAAATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑Ctt                                                                                                                          >  1:29032/1‑300 (MQ=255)                                                                                                                                                                      ttGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCCGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTTTGATATCTCNTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCNTGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑Cttt                                                                                                                         <  2:553055/300‑1 (MQ=255)                                                                                                                                                                       tGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTc                                                                                                                        >  1:668732/1‑300 (MQ=255)                                                                                                                                                                                   aCCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCATTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTaccac                                                                                                            <  1:300545/300‑1 (MQ=255)                                                                                                                                                                                   aCCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTTTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTaccac                                                                                                            <  2:128765/300‑1 (MQ=255)                                                                                                                                                                                   aCCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTaccac                                                                                                            <  2:386304/300‑1 (MQ=255)                                                                                                                                                                                     cTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGNCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTAccacca                                                                                                          <  2:610502/300‑1 (MQ=255)                                                                                                                                                                                     cTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTCCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGTGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTAccacca                                                                                                          <  2:204334/300‑1 (MQ=255)                                                                                                                                                                                      tCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACcaccac                                                                                                         <  2:121063/300‑1 (MQ=255)                                                                                                                                                                                      tCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTAGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACcaccac                                                                                                         >  2:623583/1‑300 (MQ=255)                                                                                                                                                                                          tGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAATACGGGGTGATATCTCGGGTAAGCCCTCAAGTTTGAGGAGAGTCTTTAGTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCTGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGGCGGGGACAGGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCt                                                                                                     <  1:522308/300‑1 (MQ=255)                                                                                                                                                                                          tGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCt                                                                                                     <  1:522321/300‑1 (MQ=255)                                                                                                                                                                                           gAATGAGCCGGTGAATCCAGCCCGGTCAGCCGGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCAGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAGAGGAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACCTTTTAAAATCCTTTACCCATGGTGATTGACTTTTTCTCCAGGTCGATC‑CCCCCAGCTTTCGTGCTGTACCACCACACCTc                                                                                                    >  2:36920/1‑300 (MQ=255)                                                                                                                                                                                              tGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTa                                                                                                 <  2:214438/300‑1 (MQ=255)                                                                                                                                                                                               gAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCAACACACCTCGTAg                                                                                                >  1:523972/1‑300 (MQ=255)                                                                                                                                                                                                aGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTTTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACNATGTTCGGCGAATGAGAATATTTGCCNGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGc                                                                                               <  2:466385/300‑1 (MQ=255)                                                                                                                                                                                                aGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGc                                                                                               <  2:526734/300‑1 (MQ=255)                                                                                                                                                                                                aGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATACATACAATGACCTTCCTGCACCATTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCTGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGc                                                                                               <  2:352880/300‑1 (MQ=255)                                                                                                                                                                                                                           taaaaTGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTGGCCTGCCGCCGACAATCAGGTGGTCGGGGANATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCNTAGCTGCCGTCTTCCTGCATCTCCTTCAGcc                                                                    <  2:489531/299‑1 (MQ=255)                                                                                                                                                                                                                                    gACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGtt                                                           >  1:607616/1‑300 (MQ=255)                                                                                                                                                                                                                                          gACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTGTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTCTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTcccc                                                     <  2:134700/300‑1 (MQ=255)                                                                                                                                                                                                                                              ttCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTAGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCCTGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCAGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTAAGGTCGATCACCCCCA‑CCTTCGTGCTGTACCACCACACCCCGTAGCTGCCGTCTTCCTGCATCTACTTCCGCCCGACCCGTTCTCCACTGaa                                                 >  2:266463/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAATCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCNGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGc                                              <  2:165771/300‑1 (MQ=255)                                                                                                                                                                                                                                                   gCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCt                                            >  1:16760/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cGAAACACGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGAc                             <  2:445110/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        acGGTTTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATCCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACNATCAGGTGGTCGTGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTNTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTAccc                       <  2:540186/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        acGGTGTGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTAccc                       <  2:624299/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gtgtGATATCTCGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCtt                    >  1:20897/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gtgATATCTAGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTc                  >  1:410355/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ctcGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTACTTCAGCCCGAACCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCa           >  1:357412/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGc         >  1:352872/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cGTTTAAGCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGNGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGc         >  1:472902/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGANCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGc  <  2:518097/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gCCCTCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTANCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGc  >  1:468516/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                         ATTCAAAATTGCGATATATACATACCCATGACTGCCCAGCGTATCGGCTTCACCAGTCAGCCCGTTACGGTACAGTGTGACACAGTGCGCGTACCTGGGATTCAGCTCACAGGAGAGCAGCATCAGCTCCAGATGTGCGACAAAATGCGGGAAGGCTTCATCCAGTTGTAGAGTCTGCACCTCACTGAATGAGCCGGTGAATCCAGCCCGGTCAGCCAGAAAATGCAGACGATGACCTTCCTGCACCAGTCGTGCTCCGAAACACGGTGTGATATCTCGTTTAAGCCCCCAAGTTTGAGTAGAGTCTTTATTCATAATTTTATTCCTGTTAAAGAAGACCATGTTCGGCGAATGAGAATATTTGCCTGCCGCCGACAATCAGGTGGTCGGGGACATGGTGAACACTTTTTAACATCCTTTACCCATGGTGATCGACTTTTTCTTCAGGTCGATCACCCCCA‑CTTTCGTGCTGTACCACCACACCTCGTAGCTGCCGTCTTCCTGCATCTCCTTCAGCCCGACCCGTTCTCCCCTGAACGCCTTGCCTGCGCTCAGACTTACCCCTTTCACGCTCAGCTTTCCGC  >  NC_000913/280421‑281004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑