Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,647,248	C→T	W26* (TGG→TGA)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,647,248	0	C	T	100.0%	201.7 / NA	57	W26* (TGG→TGA)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/17);  total (40/17)

AGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATC  >  NC_000913/2646954‑2647542                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       caaaaCCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGc                                                                                                                                                                                                                                                                                                   <  2:286565/297‑1 (MQ=255) aGCAACCTGCATAGTGATCGTGCTGCCACCCGCAATAACCCTTCCCGAATTGAGACCTTTCCATGCTACGCCCACCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATNAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGc                                                                                                                                                                                                                                                                                                   <  2:164865/300‑1 (MQ=255) aGCAACCTGCATAGTGAGCTTGCTGCCACCGTAAATAACCCCCCCCTAAGTTATATCTTTCCATGCTACGTTTGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGGGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGc                                                                                                                                                                                                                                                                                                   <  2:673750/300‑1 (MQ=255) aGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTTAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGc                                                                                                                                                                                                                                                                                                   <  2:673747/300‑1 (MQ=255)    aaCCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGcctg                                                                                                                                                                                                                                                                                                >  1:288786/1‑297 (MQ=255)    aaCCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGNCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGc                                                                                                                                                                                                                                                                                                >  1:494001/1‑300 (MQ=255)      ccTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCGGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCaa                                                                                                                                                                                                                                                                                              >  1:228143/1‑300 (MQ=255)      ccTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCaa                                                                                                                                                                                                                                                                                              >  1:671075/1‑300 (MQ=255)        tGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCCTCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGAACACTCGTGCGGGATTGACTTCATGTAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGa                                                                                                                                                                                                                                                                                            >  1:177178/1‑300 (MQ=255)         gCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGaa                                                                                                                                                                                                                                                                                           >  1:102014/1‑300 (MQ=255)                                               gAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCcagcag                                                                                                                                                                                                                                                     >  1:455127/1‑300 (MQ=255)                                                    gagaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCNCTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCcgcg                                                                                                                                                                                                                                                >  1:471039/1‑300 (MQ=255)                                                          ttGCCATGCTGCGCTCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATTAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGt                                                                                                                                                                                                                                          <  2:28291/300‑1 (MQ=255)                                                          ttGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTAACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGt                                                                                                                                                                                                                                          >  1:38539/1‑300 (MQ=255)                                                                           ccACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCAttt                                                                                                                                                                                                                         >  1:600274/1‑300 (MQ=255)                                                                             aCCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTCATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTAc                                                                                                                                                                                                                       >  2:188549/1‑300 (MQ=255)                                                                                  gaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATT‑ACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTg                                                                                                                                                                                                                 >  1:275126/1‑300 (MQ=255)                                                                                      ggATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACa                                                                                                                                                                                                              >  1:359831/1‑300 (MQ=255)                                                                                           cACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGAGAGGCATTTACGGTCTGACAATcag                                                                                                                                                                                                         >  2:425378/1‑300 (MQ=255)                                                                                               cccGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCNGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGa                                                                                                                                                                                                     >  2:610025/1‑300 (MQ=255)                                                                                                       cTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTTGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCAGCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGc                                                                                                                                                                                             <  2:37990/300‑1 (MQ=255)                                                                                                       cTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCNACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGc                                                                                                                                                                                             <  2:150716/300‑1 (MQ=255)                                                                                                                        cTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCAACGACCAATCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGGAAACGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCGTTTACGGTCTGACAATCAGCAGCTCTCCAGCCTCGCCGGTcgcccgcc                                                                                                                                                                            >  2:298181/1‑300 (MQ=255)                                                                                                                                          cTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCNTGCAGCGGTAGAGGCCAGAGTTTATCNGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTg                                                                                                                                                          >  2:468483/1‑300 (MQ=255)                                                                                                                                          cTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTg                                                                                                                                                          >  1:52156/1‑300 (MQ=255)                                                                                                                                          cTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTg                                                                                                                                                          >  2:130741/1‑300 (MQ=255)                                                                                                                                            tCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGNGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAt                                                                                                                                                        <  1:496038/300‑1 (MQ=255)                                                                                                                                                        ggAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATATATTGATTCCACCATCGGt                                                                                                                                            >  2:286962/1‑300 (MQ=255)                                                                                                                                                                       aTTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGGGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGANAAAGGGCGCGGCTGCCAACGTTATCCNGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAc                                                                                                                             <  2:460859/300‑1 (MQ=255)                                                                                                                                                                       aTTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAc                                                                                                                             <  2:450542/300‑1 (MQ=255)                                                                                                                                                                        ttGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAACGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGNAAGGGCGCGGCTGCCAACGTTATCCANCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACg                                                                                                                            <  2:453322/300‑1 (MQ=255)                                                                                                                                                                                     aCGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAc                                                                                                               >  1:59779/1‑300 (MQ=255)                                                                                                                                                                                                cGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCa                                                                                                    >  2:658256/1‑300 (MQ=255)                                                                                                                                                                                                   cAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGNATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGNTACGTTCCCGGCGTCACCGCCTGCGCCAGAt                                                                                                 <  2:487488/300‑1 (MQ=255)                                                                                                                                                                                                        tCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACAcc                                                                                            >  1:550383/1‑300 (MQ=255)                                                                                                                                                                                                          ggCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAg                                                                                          >  1:302505/1‑300 (MQ=255)                                                                                                                                                                                                                        gcggcgTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTNGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCNGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGt                                                                            >  2:634633/1‑300 (MQ=255)                                                                                                                                                                                                                          ggcgTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTNGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCNGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTAt                                                                          <  2:643990/300‑1 (MQ=255)                                                                                                                                                                                                                          ggcgTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTAt                                                                          <  2:108881/300‑1 (MQ=255)                                                                                                                                                                                                                           gcgTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTAtt                                                                         >  1:439024/1‑300 (MQ=255)                                                                                                                                                                                                                                  aTCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGNGTCTGGTTAACAAGCGAGGCATTTACAGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTNGACCGCCATTGGGGAACATACATTGATTCCCCCATCGGTTGCGGTACCTGATACGTTCCCGGCGACACCGCCTGCGCCAGATACACTAGCGTTACCGGTTGGTATTCATCAAc                                                                  >  2:556599/1‑300 (MQ=255)                                                                                                                                                                                                                                  aTCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAAc                                                                  >  1:301402/1‑300 (MQ=255)                                                                                                                                                                                                                                   tCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATCCACCAGCGTTACCGGTTGGTATTCATCAACg                                                                 >  2:311969/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGNATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATNCATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAACGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGc                                              >  2:543934/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGc                                              <  1:401711/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gACTGCATGCAGCGGGAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACg                                    <  2:15995/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         cAGCGGTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTAACGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAAt                           >  1:83021/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gCGGTAGAGGCAAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCCCCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCCGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCAAGGAACTCAAtgt                         >  1:230569/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gTAGAGGCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGCTTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCt                      >  1:276392/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ggCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATNCGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATg                 >  1:508238/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ggCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATg                 >  1:79746/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ggCCAGAGTTTATCTGCTCCTAACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTAACGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATg                 >  2:339542/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    gCCAGAGTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGc                <  2:396842/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gagTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGc            >  1:254435/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gagTTTATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACACTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCAGCCACAAAGCGATCGTCAAAGAACTCAATGTGCTTAATGCTCGc            >  2:318326/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             tATCTGCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCctgct       >  2:377856/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  gCTCCTCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCTGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATc  >  1:614104/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       AGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACCAGCGTTACCGGTTGGTATTCATCAACGGCAACCGCCGCCACAAAGCGATCGTCACGGAACTCAATGTGCTTAATGCTCGCCTGCTGCATC  >  NC_000913/2646954‑2647542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑