HoSeI method - Detail

~Preparation~

Only 2 plasmids needed!

• psgRNA (sgRNA expression vector)：Purchase
• pCas (Cas9 endonuclease and lambda-Red recombinase expression plasmid)：Purchase
     Reference：Jiang et al. (2015) Appl Environ Microbiol, doi: 10.1128/AEM.04023-14.